FastQCFastQC Report
Thu 26 May 2016
SRR937357_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937357_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences351631
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21630.6151334779925547No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16030.45587561961260525No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11790.33529466969635785No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5340.15186374352659462No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT4120.11716828152239137No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3990.11347122409571399No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAACGT301.3008884E-463.3243036
GGTATCA11050.052.1175581
ACCGTTG459.622666E-442.216217
GTATCAA18850.038.378961
CCGTTGG500.001615416837.9945838
GTACATG16200.031.7299731
CTAGACC1201.4783836E-731.7209113
ATCAACG23600.030.645633
TACATGG16900.030.1254832
TATCAAC24100.030.0098272
TCAACGC23800.029.988264
CAACGCA24000.029.7213845
CAATTCG404.495627E-429.68327178-79
AATTCGC404.495627E-429.68327178-79
ACATGGG16900.029.2808443
AACGCAG24700.028.8420436
TACCTGG2450.027.1893542
CGCGGAA350.00840105427.13512482-83
CGCGCTT458.951504E-426.3851362-63
TCGCGCT458.951504E-426.3851360-61
Produced by FastQC (version 0.11.5)