##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937357_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 351631 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.653824605907896 33.0 31.0 34.0 30.0 34.0 2 31.759870432356646 33.0 31.0 34.0 30.0 34.0 3 31.79725905850167 33.0 31.0 34.0 30.0 34.0 4 35.42061137954276 37.0 35.0 37.0 33.0 37.0 5 35.393864591005915 37.0 35.0 37.0 33.0 37.0 6 35.46461205070088 37.0 35.0 37.0 33.0 37.0 7 35.40558710693881 37.0 35.0 37.0 33.0 37.0 8 35.39870489234453 37.0 35.0 37.0 33.0 37.0 9 37.01678464071711 39.0 37.0 39.0 33.0 39.0 10-11 36.92707838614912 39.0 37.0 39.0 33.0 39.0 12-13 36.818684928234426 39.0 37.0 39.0 33.0 39.0 14-15 37.972884927665646 40.0 38.0 41.0 33.0 41.0 16-17 37.92268315364686 40.0 38.0 41.0 33.0 41.0 18-19 37.90360633732521 40.0 38.0 41.0 33.0 41.0 20-21 37.848656688403466 40.0 38.0 41.0 33.0 41.0 22-23 37.71751352980824 40.0 37.5 41.0 32.0 41.0 24-25 37.57681347776504 40.0 37.0 41.0 32.0 41.0 26-27 37.40824614439569 40.0 37.0 41.0 32.0 41.0 28-29 37.209209370049855 39.5 37.0 41.0 31.5 41.0 30-31 37.14819228111287 39.0 36.5 41.0 31.0 41.0 32-33 36.95931246107425 39.0 36.0 41.0 31.0 41.0 34-35 36.760413615409334 39.0 36.0 41.0 30.5 41.0 36-37 36.777175505003825 39.0 36.0 41.0 30.5 41.0 38-39 36.74172783400781 39.0 36.0 41.0 30.0 41.0 40-41 36.56301065605706 39.0 35.5 41.0 30.0 41.0 42-43 36.3091820687027 39.0 35.0 41.0 30.0 41.0 44-45 36.034384056013266 39.0 35.0 41.0 29.5 41.0 46-47 35.65566460295025 39.0 35.0 41.0 27.5 41.0 48-49 35.29139353469972 39.0 35.0 41.0 26.0 41.0 50-51 34.33501739038935 38.0 34.0 39.5 24.0 40.5 52-53 34.443865870756554 38.0 34.0 39.5 24.0 40.5 54-55 34.78585363634037 39.0 35.0 40.0 24.0 41.0 56-57 34.561163549288885 38.0 34.0 40.0 23.5 41.0 58-59 34.30327815238133 38.0 34.0 40.0 23.0 41.0 60-61 34.02006506821071 38.0 34.0 40.0 22.0 41.0 62-63 33.69899980377157 37.5 33.5 40.0 21.5 41.0 64-65 33.33726406374865 37.0 33.0 40.0 19.5 41.0 66-67 32.92635603800575 36.5 33.0 39.0 18.0 41.0 68-69 32.44541152514994 36.0 32.0 39.0 14.0 41.0 70-71 32.00912462211807 35.0 31.5 39.0 10.0 40.0 72-73 31.595822040718822 35.0 31.0 38.0 7.5 40.0 74-75 31.148405572887487 35.0 31.0 37.0 6.0 39.5 76-77 30.705998617869298 35.0 31.0 37.0 2.0 39.0 78-79 30.239331856406288 35.0 30.0 36.0 2.0 39.0 80-81 29.80246479974746 34.0 30.0 36.0 2.0 37.5 82-83 29.37665478868473 34.0 29.0 35.0 2.0 37.0 84-85 28.91441312057242 34.0 29.0 35.0 2.0 37.0 86-87 28.507203574201363 34.0 29.0 35.0 2.0 36.0 88-89 28.132093017964856 34.0 28.5 35.0 2.0 36.0 90-91 27.706678307657743 34.0 27.0 35.0 2.0 35.5 92-93 27.27691813292912 33.5 26.0 35.0 2.0 35.0 94-95 26.77126447895663 33.0 25.0 35.0 2.0 35.0 96-97 26.16649129342976 33.0 24.5 35.0 2.0 35.0 98-99 25.484305991223756 32.5 21.5 35.0 2.0 35.0 100-101 23.74337302456268 30.5 9.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17457649979655088 1101 2 0.1125220821795807 1101 3 0.2953920511915875 1101 4 0.2630423009056173 1101 5 0.35508044122724414 1101 6 0.03756102404021533 1101 7 0.08321241688824443 1101 8 0.18204123805858785 1101 9 0.009376629027535444 1101 10-11 0.12332453905221286 1101 12-13 0.22702004261301312 1101 14-15 0.22052217333260415 1101 16-17 0.18347702785361975 1101 18-19 0.2973991618314713 1101 20-21 0.3042022865579881 1101 22-23 0.26753416438854316 1101 24-25 0.32947865928687037 1101 26-27 0.42791853069058305 1101 28-29 0.45237683973177667 1101 30-31 0.5862598123121501 1101 32-33 0.5050570915582426 1101 34-35 0.09795888845169998 1101 36-37 0.30893788693287405 1101 38-39 0.6134758786794521 1101 40-41 0.5822835717391044 1101 42-43 0.7107834803502087 1101 44-45 0.5745581595327991 1101 46-47 0.8108754685431094 1101 48-49 0.8461691603483459 1101 50-51 0.6631794941033817 1101 52-53 0.6190528063470779 1101 54-55 0.7151001785621887 1101 56-57 0.7510719923736318 1101 58-59 0.8418477655870404 1101 60-61 0.923392481504834 1101 62-63 0.9736546780895736 1101 64-65 0.7355702736311116 1101 66-67 1.0919283859555264 1101 68-69 1.065206166863753 1101 70-71 0.9038410802032324 1101 72-73 1.0339748732282885 1101 74-75 0.9788215526461563 1101 76-77 1.1389698161203619 1101 78-79 1.1628859340110083 1101 80-81 1.26099431633261 1101 82-83 1.1103039774094086 1101 84-85 1.0988048226561418 1101 86-87 1.0188611794623164 1101 88-89 1.1184782647264555 1101 90-91 1.1208262583351711 1101 92-93 1.1111884775475573 1101 94-95 0.8701478771987219 1101 96-97 0.7826226410830834 1101 98-99 1.221861331723396 1101 100-101 1.8818233500232964 1102 1 0.06761205452693275 1102 2 0.14951426106721755 1102 3 0.14739287507730836 1102 4 -0.25078244351574597 1102 5 0.044484226459964304 1102 6 0.0936693824189021 1102 7 0.08067548708098826 1102 8 0.2249978854238961 1102 9 0.19683383918801667 1102 10-11 0.07119998725764987 1102 12-13 0.1740157607280537 1102 14-15 0.11560438784459137 1102 16-17 0.23763300914793462 1102 18-19 0.027203808082795433 1102 20-21 -0.006300370763575813 1102 22-23 0.13633979117992112 1102 24-25 0.11427767758642204 1102 26-27 0.008282926062896934 1102 28-29 -0.026068685176262818 1102 30-31 0.10381815154419627 1102 32-33 0.1568210482984398 1102 34-35 -0.052466979239213174 1102 36-37 -0.11261890896393822 1102 38-39 0.23602742955728928 1102 40-41 0.2512787792770794 1102 42-43 0.18441333518386216 1102 44-45 0.2351005606375054 1102 46-47 0.48685267243742913 1102 48-49 0.6007795842433552 1102 50-51 0.6355256537515359 1102 52-53 0.40224684303900204 1102 54-55 0.5163181659602145 1102 56-57 0.6835982174685 1102 58-59 0.40370809374883976 1102 60-61 0.7582251514747824 1102 62-63 0.8557635934614254 1102 64-65 0.7132441309248208 1102 66-67 0.7168457072825589 1102 68-69 0.7565985917567062 1102 70-71 0.6251776897394272 1102 72-73 0.6756382520880777 1102 74-75 0.5143552507326525 1102 76-77 0.6199167335113138 1102 78-79 0.7711514805025317 1102 80-81 0.7020734325701206 1102 82-83 0.8655308650940583 1102 84-85 0.8506281049704469 1102 86-87 0.9380304473644223 1102 88-89 1.0480889787220704 1102 90-91 0.896991157322141 1102 92-93 0.5291161148521759 1102 94-95 0.48788013174459977 1102 96-97 0.791210344298559 1102 98-99 1.1433796892898016 1102 100-101 1.2284565874300277 1103 1 0.1569270468170565 1103 2 0.03261938862172187 1103 3 0.16127308900192716 1103 4 0.10568457969194611 1103 5 -0.08343625448005554 1103 6 0.23047085803032274 1103 7 0.3201427249940494 1103 8 0.2294115445888849 1103 9 0.25819077709913074 1103 10-11 0.20390620200205234 1103 12-13 0.24925535239749053 1103 14-15 0.257319688529698 1103 16-17 0.14717373485207474 1103 18-19 0.46037432318048843 1103 20-21 0.3677590733447218 1103 22-23 0.021695665323534286 1103 24-25 -0.1161231382939718 1103 26-27 -0.039412163039820314 1103 28-29 -0.30524421657204215 1103 30-31 -0.42614544613698513 1103 32-33 -0.053874450696341114 1103 34-35 0.020967135491524402 1103 36-37 0.29425751833877456 1103 38-39 -0.001117086672472567 1103 40-41 0.24164631582578977 1103 42-43 0.4029833974484234 1103 44-45 0.4482529721691293 1103 46-47 0.45419427843356175 1103 48-49 0.3906013299931672 1103 50-51 0.3952923080563906 1103 52-53 0.22295200377524083 1103 54-55 0.45316429052836327 1103 56-57 0.5679537597947331 1103 58-59 0.6270224126448696 1103 60-61 0.5234082365956922 1103 62-63 0.3916681862043845 1103 64-65 0.6257028977352732 1103 66-67 0.991434351320585 1103 68-69 0.5716469980573322 1103 70-71 0.7221342411808784 1103 72-73 0.5800528871865538 1103 74-75 0.425746590298953 1103 76-77 0.7049095604002353 1103 78-79 0.8094373509376034 1103 80-81 0.8468848822217119 1103 82-83 0.18917602543993084 1103 84-85 0.3330051861485934 1103 86-87 0.740262407234038 1103 88-89 0.7570058389488779 1103 90-91 0.8614561993848717 1103 92-93 0.7747732108645096 1103 94-95 0.6684705227035508 1103 96-97 1.0571119604444021 1103 98-99 1.0175684445842421 1103 100-101 1.5787422919564058 1104 1 -0.15183849492362 1104 2 -0.2414747499217924 1104 3 -0.3605741578897259 1104 4 -0.25072749846080455 1104 5 -0.2103234658477291 1104 6 -0.39902292527340677 1104 7 -0.2527036337981343 1104 8 -0.26074387281786215 1104 9 -0.3689628641086813 1104 10-11 -0.2563824303247628 1104 12-13 -0.3342259975137054 1104 14-15 -0.41457418358398 1104 16-17 -0.2964741337092036 1104 18-19 -0.3527961919172 1104 20-21 -0.023772898236096296 1104 22-23 -0.27937449453436614 1104 24-25 -0.22017287186412915 1104 26-27 -0.026854436574460294 1104 28-29 -0.3866318719894437 1104 30-31 -0.6990939363678947 1104 32-33 -0.46187400664661027 1104 34-35 -0.3657224737447038 1104 36-37 -0.6754760518210858 1104 38-39 -0.24492037264050026 1104 40-41 -0.30324045149214385 1104 42-43 -0.13470754393701156 1104 44-45 -0.45432251628557196 1104 46-47 -0.13508414074939168 1104 48-49 -0.26475613004236465 1104 50-51 -0.4012463242704385 1104 52-53 -0.36109106904891064 1104 54-55 -0.5585719439298984 1104 56-57 -0.5886270572567796 1104 58-59 -0.5216765216357757 1104 60-61 -0.41100561775599687 1104 62-63 -0.33905783511000465 1104 64-65 -0.286577297646609 1104 66-67 -0.9026597872229303 1104 68-69 -0.7694453642872503 1104 70-71 -0.8256876948759562 1104 72-73 -0.5076447149448882 1104 74-75 -0.3563315624541552 1104 76-77 -0.6517178818732994 1104 78-79 -0.6242193986183473 1104 80-81 -0.2607150289683382 1104 82-83 -0.4938235305103369 1104 84-85 -0.6083691477768021 1104 86-87 -0.7512140581300812 1104 88-89 -0.9974879443548552 1104 90-91 -0.5066077437767582 1104 92-93 -0.7887547642687025 1104 94-95 -0.9853616264971592 1104 96-97 -1.355547897459683 1104 98-99 -0.9889280030178895 1104 100-101 -1.026419786196346 1105 1 -0.1418179187919293 1105 2 -0.11096034662961074 1105 3 -0.01983341714898401 1105 4 -0.3484229717118339 1105 5 -0.25379054403703094 1105 6 -0.32822045613760054 1105 7 -0.35759046507385506 1105 8 -0.24775004565736936 1105 9 -0.27395257604283785 1105 10-11 -0.17225691592147285 1105 12-13 -0.24986385759601148 1105 14-15 -0.4599394099214251 1105 16-17 -0.4772405946145497 1105 18-19 -0.18112952525054027 1105 20-21 -0.29689018218672203 1105 22-23 -0.11516667560432836 1105 24-25 -0.15688069079417488 1105 26-27 -0.09998200859092066 1105 28-29 -0.387778991331011 1105 30-31 -0.5082708911004019 1105 32-33 -0.20547997372479898 1105 34-35 -0.13236342024676873 1105 36-37 -0.08263654564824918 1105 38-39 -0.6068082327228126 1105 40-41 -0.18730938153330356 1105 42-43 -0.12269108303166831 1105 44-45 -0.14300564385759174 1105 46-47 0.29892306089670484 1105 48-49 0.20465230617163144 1105 50-51 0.10554894321927577 1105 52-53 -0.09806123365796537 1105 54-55 0.5949568626544632 1105 56-57 1.0490478398625598 1105 58-59 0.5010292396810954 1105 60-61 0.21070220117405114 1105 62-63 0.2029123294990498 1105 64-65 0.07068093280606291 1105 66-67 0.3828340399375634 1105 68-69 0.5520978455892944 1105 70-71 0.7493071610911208 1105 72-73 0.2729056965777481 1105 74-75 0.19885362273102558 1105 76-77 0.6169292374682662 1105 78-79 0.6656931528219836 1105 80-81 0.515740732348533 1105 82-83 0.6322207081727882 1105 84-85 1.0294652143631104 1105 86-87 1.3965277114172459 1105 88-89 1.391730162641032 1105 90-91 0.9663140669228305 1105 92-93 1.3650158118629818 1105 94-95 1.429484052515189 1105 96-97 1.1289274111822927 1105 98-99 0.8294156183812831 1105 100-101 0.5402726006349319 1106 1 -0.36529260120381224 1106 2 -0.48526702045319325 1106 3 -0.5625065250394812 1106 4 -0.19401252261539526 1106 5 -0.306036026234203 1106 6 -0.23696978517678957 1106 7 0.08721182513906456 1106 8 -0.20051440421882916 1106 9 -0.3736126225627885 1106 10-11 -0.5320708361218678 1106 12-13 -0.8100714081417308 1106 14-15 -0.44133142996079044 1106 16-17 -0.6805864525497825 1106 18-19 -0.6907913609993344 1106 20-21 -0.6578671011346486 1106 22-23 -0.7843382626503086 1106 24-25 -0.9732163501250071 1106 26-27 -0.4844872868160124 1106 28-29 -0.4616741425208417 1106 30-31 -0.32890069965291246 1106 32-33 -0.14419888587366358 1106 34-35 -0.22692416456596476 1106 36-37 -0.5839784672800263 1106 38-39 -0.4375471842347096 1106 40-41 -0.3587175046322457 1106 42-43 -0.3251423265457163 1106 44-45 -0.44296985928073696 1106 46-47 -0.2408872808460032 1106 48-49 -0.07697231361724022 1106 50-51 -0.05324511654097108 1106 52-53 0.10277970389794433 1106 54-55 -0.1439946492439006 1106 56-57 -0.6234338205417984 1106 58-59 -0.6081137197034039 1106 60-61 -0.40774474819077255 1106 62-63 -0.29744551626942695 1106 64-65 -0.15010990634226573 1106 66-67 -0.45398828963838866 1106 68-69 -0.6891796638041576 1106 70-71 -0.18637006202571982 1106 72-73 -0.10963142992073216 1106 74-75 -0.22063107936237003 1106 76-77 -0.2970983166559087 1106 78-79 0.06594364485991022 1106 80-81 -0.37842638162534215 1106 82-83 -0.23689720200792053 1106 84-85 -0.21190175647245724 1106 86-87 -0.1483819325262168 1106 88-89 -0.2563285240649975 1106 90-91 -0.35163189836613284 1106 92-93 -0.4429999333508299 1106 94-95 -0.17706452504788572 1106 96-97 -0.4623232597785254 1106 98-99 -0.8233845247570208 1106 100-101 -0.5905200277422402 1107 1 -0.055727383812506304 1107 2 0.05019191674487189 1107 3 -0.04501860233416721 1107 4 -0.3062830540163617 1107 5 0.03328764526338546 1107 6 0.2737548525043749 1107 7 0.31812969953519854 1107 8 0.058422793848805554 1107 9 0.2157593581135373 1107 10-11 0.18000645856412234 1107 12-13 0.439107335819628 1107 14-15 0.4263980386382471 1107 16-17 0.6281091996241273 1107 18-19 0.6433149191939052 1107 20-21 0.5559493239861197 1107 22-23 0.33034772768785103 1107 24-25 0.18649379480253714 1107 26-27 0.30759000786998314 1107 28-29 0.25378479467722315 1107 30-31 0.5997949525210018 1107 32-33 0.4504871044645 1107 34-35 0.4719164151441859 1107 36-37 0.022301725956687335 1107 38-39 -0.23117037264051277 1107 40-41 -0.09949045149215152 1107 42-43 0.0919591227296479 1107 44-45 0.22456637260332002 1107 46-47 0.3639436370283917 1107 48-49 0.7306605366243062 1107 50-51 0.6269481201740135 1107 52-53 0.6465478198399737 1107 54-55 0.39948361162566215 1107 56-57 0.4185951649654456 1107 58-59 0.6641568116975591 1107 60-61 0.3384388266884528 1107 62-63 0.5116366093344382 1107 64-65 0.22467270235338788 1107 66-67 0.129006879443736 1107 68-69 0.19333241349053054 1107 70-71 -0.08221547265373985 1107 72-73 -0.1830613816115516 1107 74-75 -0.17744267356527033 1107 76-77 -0.07796788187329895 1107 78-79 -0.3568582875072366 1107 80-81 -0.0789094734127822 1107 82-83 -0.3042401971770019 1107 84-85 -0.36823025888791605 1107 86-87 -0.15524183590785867 1107 88-89 -0.1574879443548518 1107 90-91 0.19117003400101495 1107 92-93 -0.17556031982426035 1107 94-95 -0.5584171820527146 1107 96-97 -0.6730478974596821 1107 98-99 -0.5119835585734478 1107 100-101 0.17788576935921085 1108 1 -0.3918214300772007 1108 2 -0.17674778746445696 1108 3 -0.14623968689996403 1108 4 -0.1781677714983445 1108 5 -0.07359138393646703 1108 6 0.030857620801683083 1108 7 0.031895342311081265 1108 8 -0.26831213220352623 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-1.2869199643795035 2316 72-73 -1.6628486156541058 2316 74-75 -1.5944875908229506 2316 76-77 -1.8168331301002496 2316 78-79 -1.6452034411715388 2316 80-81 -2.010351553791036 2316 82-83 -1.6767933886663648 2316 84-85 -1.4278283676349623 2316 86-87 -1.939804483661998 2316 88-89 -1.8842964549931516 2316 90-91 -1.6978134175356239 2316 92-93 -2.073409019587853 2316 94-95 -1.8754148179865204 2316 96-97 -1.2772322946228023 2316 98-99 -1.2382010526632818 2316 100-101 -1.5711449635013075 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1558.0 3 278.0 4 234.0 5 290.0 6 337.0 7 405.0 8 495.0 9 544.0 10 778.0 11 951.0 12 1285.0 13 1598.0 14 1990.0 15 2460.0 16 3210.0 17 4387.0 18 3685.0 19 2525.0 20 2369.0 21 2568.0 22 2614.0 23 2932.0 24 3200.0 25 3601.0 26 4189.0 27 4678.0 28 5533.0 29 6683.0 30 8064.0 31 10094.0 32 12606.0 33 16644.0 34 22463.0 35 32089.0 36 48019.0 37 68557.0 38 58504.0 39 9210.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.835004766008936 18.911066843988834 15.383078670540357 22.87084971946187 2 14.73298339704456 19.667370342899527 41.124265419067726 24.47538084098819 3 18.52021967049426 24.73974752157478 30.553027601454964 26.187005206475998 4 12.035787545683043 16.231067036030048 37.86450145928967 33.86864395899724 5 13.104036465390113 37.10747078404582 34.789305691441875 14.999187059122196 6 30.953724604966137 36.19918143055841 17.894235811843036 14.95285815263242 7 27.604214174952112 32.95431223205327 21.086267445042413 18.355206147952202 8 26.149824989453762 34.10453887286368 20.025596004970982 19.72004013271158 9 25.860579924124742 16.333417892537604 20.56470346797249 37.24129871536517 10-11 24.322617885367748 26.423140236732774 27.704005643960176 21.550236233939298 12-13 25.58421587448177 23.715950728764497 28.439986489317583 22.25984690743615 14-15 22.135980711794076 25.004844850778596 27.316123663391167 25.543050774036157 16-17 21.08376041090658 28.604553720563004 27.773248886937985 22.538436981592433 18-19 20.99528605710312 26.5686542747539 30.912806306537387 21.52325336160559 20-21 21.70235202747476 25.565491246695686 31.023534525141255 21.708622200688293 22-23 21.313776455365744 25.376203423451155 31.185411287242715 22.12460883394039 24-25 21.245919671493482 25.760604579656704 31.037425749063534 21.956049999786277 26-27 20.893816533895766 25.979250687605994 31.052999102193212 22.07393367630503 28-29 20.626589061555826 26.187876956754724 31.21372949184234 21.97180448984711 30-31 20.85802569430786 25.860063793943848 31.08743304913958 22.19447746260871 32-33 20.63360850132263 26.001379640609322 30.989036988050717 22.37597487001733 34-35 21.301840920945 26.110210142593704 30.562736385478985 22.02521255098231 36-37 21.09807397442986 25.89988543026351 30.889653953795904 22.112386641510724 38-39 20.8695838023453 26.248806691079036 30.67566219739823 22.205947309177436 40-41 21.04002576056354 26.176750521480436 30.84343048637312 21.939793231582907 42-43 20.906162147682373 26.193437890504217 30.35791381513984 22.542486146673564 44-45 20.9403475569262 26.399676213983792 30.218413049488525 22.44156317960148 46-47 21.057259055115864 26.673440131783323 29.703259528213426 22.566041284887383 48-49 21.161369210576193 26.812305736587007 29.470605923706945 22.555719129129855 50-51 21.144949389856308 27.018348493097584 29.39436005461506 22.442342062431052 52-53 21.3812390157546 26.95499969354579 29.194253484669453 22.469507806030165 54-55 20.98377245807707 26.88669227534796 29.363512617858134 22.766022648716834 56-57 21.143215364449762 26.994642958910354 29.23063771584886 22.63150396079102 58-59 21.313953256462636 26.79191632547816 29.17140341729847 22.722727000760727 60-61 21.070575278920305 26.753269326388402 29.706953809856916 22.469201584834373 62-63 21.067823534859205 26.688179855961362 29.730230305102616 22.51376630407682 64-65 20.851171743104103 26.734035899547376 29.693989055467778 22.720803301880743 66-67 20.90591093348152 26.81855421858414 29.329400785084815 22.94613406284953 68-69 20.86967547806364 26.460405702285527 29.71303359196688 22.95688522768395 70-71 20.9489601246967 26.5989231516692 29.464867075198438 22.98724964843566 72-73 20.844821960127163 26.439017300268887 29.530295063559713 23.185865676044237 74-75 20.641506423043165 26.777877831097328 29.31346958315761 23.267146162701895 76-77 20.672554870593466 26.4940676202935 29.458119935816867 23.375257573296167 78-79 20.828702199574792 26.467855656823012 29.281732528513533 23.421709615088663 80-81 20.805752566685378 26.530394171066767 29.090810653703354 23.573042608544505 82-83 20.74686904641463 26.313689559308216 29.401111169692058 23.538330224585092 84-85 20.660359885452138 26.26593910726752 29.283078545071167 23.79062246220918 86-87 20.773569770738735 26.356283206266752 29.164381082917888 23.705765940076624 88-89 20.702729814222167 26.03426018016375 29.054753022915165 24.208256982698924 90-91 20.491711014697188 25.93971517521925 29.501805980038043 24.066767830045524 92-93 20.52065002885376 25.74040167280551 29.497944615033877 24.241003683306854 94-95 20.309031270750037 25.72662530670869 29.320068508961167 24.644274913580105 96-97 20.115009674376722 25.844691346063424 29.455556283785516 24.584742695774334 98-99 20.322318642328902 26.220521808518214 29.099445703130566 24.357713846022314 100-101 20.30071546003425 26.15617600715745 29.090530910665795 24.452577622142506 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 553.0 1 323.0 2 103.5 3 124.5 4 112.5 5 101.0 6 169.5 7 378.5 8 637.5 9 739.0 10 655.0 11 561.5 12 574.5 13 618.0 14 652.0 15 695.0 16 712.0 17 715.5 18 763.5 19 839.5 20 916.5 21 1010.0 22 1170.5 23 1376.5 24 1598.0 25 1833.5 26 2167.0 27 2700.0 28 3194.5 29 3652.5 30 4239.0 31 4853.5 32 5671.0 33 6359.0 34 6947.5 35 7553.5 36 8229.0 37 8953.0 38 9565.0 39 9890.0 40 10189.5 41 10808.0 42 11283.5 43 11741.0 44 12273.0 45 12694.0 46 12826.5 47 13388.5 48 14571.0 49 14641.0 50 13510.0 51 12587.5 52 11950.5 53 11685.0 54 11497.5 55 10598.0 56 9403.0 57 8256.5 58 7289.0 59 6367.0 60 5277.0 61 4337.0 62 3415.0 63 2538.0 64 1883.5 65 1372.0 66 996.5 67 803.5 68 576.5 69 341.5 70 257.0 71 184.0 72 130.0 73 99.5 74 66.0 75 49.0 76 33.5 77 23.5 78 16.5 79 11.5 80 6.5 81 5.0 82 3.5 83 2.0 84 2.5 85 3.0 86 1.5 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35065167746871007 2 0.3102684348080801 3 0.31453427030040015 4 0.3179469386942562 5 0.2991772625280479 6 0.22040150043653714 7 0.2317770617493907 8 0.22608928109296395 9 0.22523611399449991 10-11 0.2313504782001587 12-13 0.22680025367501727 14-15 0.21130105138625435 16-17 0.2266580591586066 18-19 0.2152824978457531 20-21 0.21713102655909178 22-23 0.2152824978457531 24-25 0.20177401878673948 26-27 0.22068588946935852 28-29 0.22608928109296395 30-31 0.23149267271656937 32-33 0.2317770617493907 34-35 0.22011711140371581 36-37 0.21414494171446774 38-39 0.20362254750007822 40-41 0.20106304620468615 42-43 0.2054710762134169 44-45 0.22239222366628653 46-47 0.2138605526816464 48-49 0.22367197431398256 50-51 0.23092389465092666 52-53 0.24016653821762016 54-55 0.23846020402069215 56-57 0.19651282167954476 58-59 0.18542164939951256 60-61 0.19324234780209937 62-63 0.19437990393338472 64-65 0.19324234780209937 66-67 0.2029115749180249 68-69 0.19452209844979537 70-71 0.19893012845852615 72-73 0.210590078804201 74-75 0.20106304620468615 76-77 0.21613566494421707 78-79 0.211869829451897 80-81 0.19679721071236608 82-83 0.21030568977137964 84-85 0.19509087651543805 86-87 0.19893012845852615 88-89 0.20703521589393428 90-91 0.2029115749180249 92-93 0.20575546524623822 94-95 0.20703521589393428 96-97 0.20020987910622212 98-99 0.20931032815650497 100-101 0.18997187392465398 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 351631.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.00771626597957 #Duplication Level Percentage of deduplicated Percentage of total 1 87.57007713798346 64.80861422217824 2 7.850207751323358 11.619518957778654 3 1.9849713048432198 4.407095793748447 4 0.8626487753289567 2.553706632069607 5 0.46722046391714006 1.7288959763619525 6 0.28083323759568624 1.2470295939622784 7 0.1843965557685669 0.9552737585820783 8 0.13046503904156578 0.7724335673614517 9 0.10139089612738526 0.6753337805294042 >10 0.5239873254050958 6.664214880975829 >50 0.03035367983022465 1.5298312786908306 >100 0.011910937654162177 1.4800691087745452 >500 3.8422379529555413E-4 0.15184570683681214 >1k 0.0011526713858866623 1.40613674214988 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2163 0.6151334779925547 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1603 0.45587561961260525 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1179 0.33529466969635785 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 534 0.15186374352659462 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 412 0.11716828152239137 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 399 0.11347122409571399 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.843890328213383E-4 0.0 16-17 0.0 0.0 0.0 2.843890328213383E-4 0.0 18-19 0.0 0.0 0.0 2.843890328213383E-4 0.0 20-21 0.0 0.0 0.0 2.843890328213383E-4 0.0 22-23 0.0 0.0 0.0 2.843890328213383E-4 0.0 24-25 0.0 0.0 0.0 8.531670984640149E-4 0.0 26-27 0.0 0.0 0.0 0.001137556131285353 0.0 28-29 0.0 0.0 0.0 0.0027016958118027136 0.0 30-31 0.0 0.0 0.0 0.00654094775489078 0.0 32-33 0.0 0.0 0.0 0.009527032599514831 0.0 34-35 0.0 0.0 0.0 0.012939700993370891 0.0 36-37 0.0 0.0 0.0 0.017916509067744314 0.0 38-39 0.0 0.0 0.0 0.02630598553597379 0.0 40-41 0.0 0.0 0.0 0.04308493847243275 0.0 42-43 0.0 0.0 0.0 0.07934454015715338 0.0 44-45 0.0 0.0 0.0 0.11404000216135665 0.0 46-47 0.0 0.0 0.0 0.19338454231851004 0.0 48-49 0.0 0.0 0.0 0.2718759153771994 0.0 50-51 0.0 0.0 0.0 0.3364322258276432 0.0 52-53 0.0 0.0 0.0 0.4261569656827754 0.0 54-55 0.0 0.0 0.0 0.5130378152096943 0.0 56-57 0.0 0.0 0.0 0.7139586668979698 0.0 58-59 0.0 0.0 0.0 0.9123200172908532 0.0 60-61 0.0 0.0 0.0 1.147936330983332 0.0 62-63 0.0 0.0 0.0 1.3940750388902003 0.0 64-65 0.0 0.0 0.0 1.6642446200704715 0.0 66-67 0.0 0.0 0.0 2.0463212856659396 0.0 68-69 0.0 0.0 0.0 2.5397362576109614 0.0 70-71 0.0 0.0 0.0 2.9427155171187978 0.0 72-73 0.0 0.0 0.0 3.349534028569722 0.0 74-75 0.0 0.0 0.0 3.8304358830706056 0.0 76-77 0.0 0.0 0.0 4.366082626389597 0.0 78-79 0.0 0.0 0.0 4.958180592723622 0.0 80-81 0.0 0.0 0.0 5.73328290167818 0.0 82-83 0.0 0.0 0.0 6.4654424666767145 0.0 84-85 0.0 0.0 0.0 7.21665609687428 0.0 86-87 0.0 0.0 0.0 7.9965930193868004 0.0 88-89 0.0 0.0 0.0 9.233685312159622 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCAACGT 30 1.3008884E-4 63.324303 6 GGTATCA 1105 0.0 52.117558 1 ACCGTTG 45 9.622666E-4 42.21621 7 GTATCAA 1885 0.0 38.37896 1 CCGTTGG 50 0.0016154168 37.994583 8 GTACATG 1620 0.0 31.729973 1 CTAGACC 120 1.4783836E-7 31.720911 3 ATCAACG 2360 0.0 30.64563 3 TACATGG 1690 0.0 30.125483 2 TATCAAC 2410 0.0 30.009827 2 TCAACGC 2380 0.0 29.98826 4 CAACGCA 2400 0.0 29.721384 5 CAATTCG 40 4.495627E-4 29.683271 78-79 AATTCGC 40 4.495627E-4 29.683271 78-79 ACATGGG 1690 0.0 29.280844 3 AACGCAG 2470 0.0 28.842043 6 TACCTGG 245 0.0 27.189354 2 CGCGGAA 35 0.008401054 27.135124 82-83 CGCGCTT 45 8.951504E-4 26.38513 62-63 TCGCGCT 45 8.951504E-4 26.38513 60-61 >>END_MODULE