FastQCFastQC Report
Thu 26 May 2016
SRR937357_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937357_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences351631
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22500.6398753238480112No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18280.5198631519974063No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12940.36799940847081175No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT7750.22040150043653714No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4240.12058094991624743No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC4220.12001217185060475No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGGT1700.044.707694
AGGTAAG1600.044.533052
TAAGGTG1850.041.082745
CAGTCCG608.277035E-539.584939
GGTAAGG2000.038.0015373
GGTGTGC3900.035.3219388
GGGCGTA550.002576690234.5468529
AAGGTAA2150.030.9667191
AAGGTGT2500.030.3969046
AGGTGTG3000.028.5011527
CTTCGTA350.00837610527.1516890-91
GGTATCA21700.026.2982671
GTATCAA30150.025.8679561
GGTGTAG1551.3916215E-624.5450461
ACGAATC1002.0534571E-823.74758372-73
GTACATG19250.022.7280771
AGGACGA1050.002192697322.619965
GAGTACT20100.022.21482712-13
TTGCGTA1306.2391337E-1021.92084560-61
GTGTGCA3700.021.8252079
Produced by FastQC (version 0.11.5)