##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937357_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 351631 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.128273673254064 33.0 31.0 34.0 31.0 34.0 2 32.3394922518208 34.0 31.0 34.0 31.0 34.0 3 32.410848304046006 34.0 31.0 34.0 31.0 34.0 4 36.03812803763036 37.0 35.0 37.0 35.0 37.0 5 35.92359604244222 37.0 35.0 37.0 35.0 37.0 6 36.011526287500246 37.0 35.0 37.0 35.0 37.0 7 35.96813989665303 37.0 35.0 37.0 35.0 37.0 8 35.93887342128538 37.0 35.0 37.0 35.0 37.0 9 37.64178073036791 39.0 37.0 39.0 35.0 39.0 10-11 37.55230625286166 39.0 37.0 39.0 35.0 39.0 12-13 37.472832315694575 39.0 37.0 39.0 35.0 39.0 14-15 38.65175567569412 40.0 38.0 41.0 34.0 41.0 16-17 38.62397513302297 40.0 38.0 41.0 34.0 41.0 18-19 38.64159872138691 40.0 38.0 41.0 34.0 41.0 20-21 38.548090185450086 40.0 38.0 41.0 34.0 41.0 22-23 38.42226652371378 40.0 38.0 41.0 34.0 41.0 24-25 38.28591620192759 40.0 38.0 41.0 34.0 41.0 26-27 38.199761966379526 40.0 38.0 41.0 34.0 41.0 28-29 38.09748998239632 40.0 38.0 41.0 33.0 41.0 30-31 38.157514553608756 40.0 37.5 41.0 34.0 41.0 32-33 38.07920518953107 40.0 37.0 41.0 33.5 41.0 34-35 37.949793960145726 40.0 37.0 41.0 33.0 41.0 36-37 37.774173778762396 40.0 37.0 41.0 33.0 41.0 38-39 37.630717996991166 40.0 37.0 41.0 33.0 41.0 40-41 37.450972752686766 40.0 36.5 41.0 33.0 41.0 42-43 37.22511809254588 40.0 36.0 41.0 32.5 41.0 44-45 36.86398952310803 40.0 36.0 41.0 31.0 41.0 46-47 36.45209040158575 39.0 35.5 41.0 30.5 41.0 48-49 36.08948727501273 39.0 35.0 41.0 29.5 41.0 50-51 35.83315748611471 39.0 35.0 41.0 29.0 41.0 52-53 35.57880846683028 39.0 35.0 40.0 28.0 41.0 54-55 35.33768922535271 39.0 35.0 40.0 27.5 41.0 56-57 35.06299359271509 38.0 34.5 40.0 26.5 41.0 58-59 34.805298736459534 38.0 34.0 40.0 26.0 41.0 60-61 34.43773728709926 38.0 34.0 40.0 26.0 41.0 62-63 34.056741299828516 37.0 34.0 40.0 25.0 41.0 64-65 33.69995250703152 36.5 33.0 39.5 24.5 41.0 66-67 33.271615699412166 36.0 33.0 39.0 22.5 40.5 68-69 32.86494222636799 35.5 32.5 39.0 22.0 40.0 70-71 32.43298372441565 35.0 32.0 38.0 20.5 40.0 72-73 31.982346550787618 35.0 31.5 37.5 19.5 39.5 74-75 31.534076915857817 35.0 31.0 37.0 18.0 39.0 76-77 30.201710031254354 33.5 29.5 35.5 16.5 38.0 78-79 30.832548893584466 34.0 31.0 36.0 17.0 38.5 80-81 30.700701872133003 34.0 31.0 36.0 14.0 37.0 82-83 30.38773742929378 34.0 31.0 35.0 9.5 37.0 84-85 30.04278632998797 34.0 30.5 35.0 7.0 36.5 86-87 29.661295505800112 34.0 30.0 35.0 2.0 36.0 88-89 29.292815479863833 34.0 30.0 35.0 2.0 36.0 90-91 28.859955464677462 34.0 29.0 35.0 2.0 35.0 92-93 28.49471747371534 34.0 29.0 35.0 2.0 35.0 94-95 28.082794179125276 34.0 29.0 35.0 2.0 35.0 96-97 27.602633158054893 33.0 27.5 35.0 2.0 35.0 98-99 27.101751552053145 33.0 27.0 35.0 2.0 35.0 100-101 25.276091982788778 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2413460623985273 1101 2 0.16254352425748664 1101 3 0.10817086347539373 1101 4 0.07635852379748087 1101 5 0.06643392605832332 1101 6 0.12038179126893311 1101 7 0.03973008146169832 1101 8 0.09042947077578134 1101 9 0.28110742896419794 1101 10-11 0.15955279715561232 1101 12-13 0.08564701802963981 1101 14-15 0.04596783653335024 1101 16-17 0.048450153487543446 1101 18-19 -0.04164295614239677 1101 20-21 0.10330627729421593 1101 22-23 0.049615964222553544 1101 24-25 0.16320899689764445 1101 26-27 -0.03741557280304875 1101 28-29 0.019944817329488274 1101 30-31 0.031446319125571165 1101 32-33 0.020505693304315287 1101 34-35 -0.040106893514362696 1101 36-37 0.04151059444908611 1101 38-39 -0.1874319047057469 1101 40-41 0.009046199289464596 1101 42-43 0.17881557389297598 1101 44-45 0.054400518516665386 1101 46-47 0.0477409737761576 1101 48-49 0.1741306364465416 1101 50-51 0.34190783806440095 1101 52-53 0.2517601014707509 1101 54-55 0.32460410204495105 1101 56-57 0.36045041117419885 1101 58-59 0.3035041873690929 1101 60-61 0.21808866359429402 1101 62-63 0.2966888966958834 1101 64-65 0.1845498299139834 1101 66-67 0.1548934855598887 1101 68-69 0.3319206546260034 1101 70-71 0.14571747052740136 1101 72-73 0.15707878716090207 1101 74-75 0.2371177830536979 1101 76-77 0.21551972804210706 1101 78-79 0.1061625603094889 1101 80-81 0.14486772915660495 1101 82-83 0.24914083434051904 1101 84-85 0.14458508108540968 1101 86-87 0.03665867383305965 1101 88-89 0.20179369993925889 1101 90-91 0.15630236292276933 1101 92-93 0.028242413881937267 1101 94-95 0.0953068160367998 1101 96-97 -0.011111429374754067 1101 98-99 0.12434179551098268 1101 100-101 0.6496894131290034 1102 1 0.12321709527272873 1102 2 0.1158271730268794 1102 3 0.09908346196454687 1102 4 0.09786513228930716 1102 5 0.026899015351290245 1102 6 0.13472001376449327 1102 7 0.04480394107621066 1102 8 0.07437530319046459 1102 9 -0.04641760671817963 1102 10-11 0.02965227265370629 1102 12-13 0.14051084480477272 1102 14-15 0.025042879758331082 1102 16-17 0.016317159765321776 1102 18-19 0.2675266888904986 1102 20-21 0.19685139374059446 1102 22-23 0.0675412807223168 1102 24-25 0.10901022576381791 1102 26-27 0.12844219667140777 1102 28-29 0.24568805508434366 1102 30-31 0.3109887144282695 1102 32-33 0.08179960003116804 1102 34-35 -0.002977642089966537 1102 36-37 0.11560228944891549 1102 38-39 0.10726969781001117 1102 40-41 0.2374576373761883 1102 42-43 0.24214236062260852 1102 44-45 0.19833320937384968 1102 46-47 0.049293737274538785 1102 48-49 0.22813213916768404 1102 50-51 0.24933383339958937 1102 52-53 0.24169941242379167 1102 54-55 0.1717921568623595 1102 56-57 0.31980151413669944 1102 58-59 0.4611180228472378 1102 60-61 0.4453070629351785 1102 62-63 0.3118473786574256 1102 64-65 0.18693808316022142 1102 66-67 0.14069523660715078 1102 68-69 0.38546162263370576 1102 70-71 0.2635171734643791 1102 72-73 0.521517674567054 1102 74-75 0.4197027535214559 1102 76-77 0.43936643307941736 1102 78-79 0.23877915174340103 1102 80-81 0.17350863682338158 1102 82-83 0.3731443387558535 1102 84-85 0.33237228068622926 1102 86-87 0.20455004856181347 1102 88-89 0.5641155781666285 1102 90-91 0.6469212345474524 1102 92-93 0.3799705431971816 1102 94-95 0.45423959392417146 1102 96-97 0.33571980169618243 1102 98-99 0.14792552434814255 1102 100-101 0.7092637485085156 1103 1 -0.03617236074195063 1103 2 -0.022016111574842512 1103 3 -0.025404172958253923 1103 4 0.059425552331077824 1103 5 -0.04215803771997173 1103 6 -0.011760282138538969 1103 7 -0.09310033936803563 1103 8 0.003493254510900101 1103 9 -0.06099561308126056 1103 10-11 -0.06909389680737377 1103 12-13 -8.53119827404214E-4 1103 14-15 -0.10796221461443167 1103 16-17 -0.029393124621691413 1103 18-19 0.01575902937416629 1103 20-21 -0.10290535483941454 1103 22-23 0.009282931611387824 1103 24-25 -0.14048350874323745 1103 26-27 -0.3395691436756394 1103 28-29 -0.25665174543485136 1103 30-31 -0.1973091599886132 1103 32-33 -0.1980127279605597 1103 34-35 -0.12174264706722937 1103 36-37 -0.22839474371330226 1103 38-39 -0.08959560385103771 1103 40-41 -0.13767777339420917 1103 42-43 -0.43080891101871543 1103 44-45 -0.6685921609929082 1103 46-47 -0.408737463318829 1103 48-49 -0.2228601411984883 1103 50-51 0.15859641806181202 1103 52-53 0.09950590433329864 1103 54-55 -0.11959474220659416 1103 56-57 0.17417272019529406 1103 58-59 0.4759192739939522 1103 60-61 -0.05584453857165528 1103 62-63 0.01504038902184135 1103 64-65 0.00258790846812218 1103 66-67 0.5299865625103735 1103 68-69 0.28671667308947235 1103 70-71 0.17379716877989893 1103 72-73 0.39007407376288583 1103 74-75 0.3312319436918507 1103 76-77 0.31153012640207933 1103 78-79 0.4833188039207066 1103 80-81 0.41162298694927557 1103 82-83 0.5722786857587607 1103 84-85 0.5556963589170607 1103 86-87 0.5564350640791531 1103 88-89 0.6545687528782409 1103 90-91 0.2600111863363388 1103 92-93 0.22685877929501075 1103 94-95 0.20302943640851012 1103 96-97 0.39970444050320353 1103 98-99 0.7372551556404794 1103 100-101 0.7135200383997962 1104 1 -0.10656312450749184 1104 2 -0.1018651346654238 1104 3 -0.2113011534200666 1104 4 -0.19839860397443232 1104 5 -0.09216691871464633 1104 6 -0.14077449173001355 1104 7 -0.1209378171655473 1104 8 -0.06513019131503484 1104 9 -0.18273628803685682 1104 10-11 -0.1561032218517795 1104 12-13 -0.3591457486018257 1104 14-15 -0.5400670103515495 1104 16-17 -0.29669108199291827 1104 18-19 -0.3131463880325782 1104 20-21 -0.6418573974681934 1104 22-23 -0.4749999280688897 1104 24-25 -0.5076793346757427 1104 26-27 -0.5616539571747481 1104 28-29 -0.4386388679925801 1104 30-31 -0.1755085383189865 1104 32-33 -0.4412498505182896 1104 34-35 -0.33406280692513945 1104 36-37 -0.7241229852763595 1104 38-39 -0.7675929395526282 1104 40-41 -0.7427621428435955 1104 42-43 -0.8328026943224387 1104 44-45 -0.9279305268898881 1104 46-47 -0.8056513176705096 1104 48-49 -0.9346563223707776 1104 50-51 -0.6442788039630472 1104 52-53 -0.611679708455334 1104 54-55 -0.4900210299508245 1104 56-57 -0.7747726616874786 1104 58-59 -0.6773023068231012 1104 60-61 -0.8460665634384412 1104 62-63 -0.7620399839799319 1104 64-65 -1.0804382904661551 1104 66-67 -0.7818184996565876 1104 68-69 -0.49128288286080135 1104 70-71 -0.6386806287334252 1104 72-73 -0.5307762814768999 1104 74-75 -0.6777423014235993 1104 76-77 -0.6299330174700337 1104 78-79 -0.47565766144341026 1104 80-81 -0.47805729724255386 1104 82-83 -0.6715534634419491 1104 84-85 -0.6246744226104717 1104 86-87 -0.5406697316579674 1104 88-89 -0.29183222403117526 1104 90-91 -0.2161144797382626 1104 92-93 -0.16176022603358575 1104 94-95 -0.21091150497692723 1104 96-97 -0.061656571930189585 1104 98-99 -0.2546431569705341 1104 100-101 0.43985440784917174 1105 1 -0.09656312450749027 1105 2 -0.07818200709341028 1105 3 -0.012700330374798341 1105 4 -0.12450971508554431 1105 5 -0.20346321501094167 1105 6 -0.13157696086581439 1105 7 -0.20623617107501246 1105 8 -0.08062401847552536 1105 9 0.022778115255320586 1105 10-11 0.14548628432105914 1105 12-13 0.012124827529866877 1105 14-15 -0.06156906796472583 1105 16-17 -0.04488552643736199 1105 18-19 -0.10543548268278613 1105 20-21 -0.2643676855340331 1105 22-23 -0.3747890227191064 1105 24-25 -0.17133674208314886 1105 26-27 -0.06604181725705871 1105 28-29 -0.40218310667570734 1105 30-31 -0.03589434078812559 1105 32-33 0.059748091868549125 1105 34-35 -0.1454362637152613 1105 36-37 0.18861364023803873 1105 38-39 0.12834327443913907 1105 40-41 0.5603757172387134 1105 42-43 0.08868907522487746 1105 44-45 0.3301250286656696 1105 46-47 0.27649888809079926 1105 48-49 -0.23366352401686896 1105 50-51 0.11094238945258894 1105 52-53 0.003536340927382753 1105 54-55 0.09866209762119382 1105 56-57 0.10282507493803905 1105 58-59 0.48715756972011093 1105 60-61 -0.026745575784126174 1105 62-63 0.0770392341270636 1105 64-65 0.035791133402149455 1105 66-67 0.0554088665985617 1105 68-69 -0.14266662771676408 1105 70-71 0.013274103776858226 1105 72-73 -0.05489665184727244 1105 74-75 0.46403753396734615 1105 76-77 0.31263385495794793 1105 78-79 0.2856746430833361 1105 80-81 0.3276628673665023 1105 82-83 0.3476955077514674 1105 84-85 0.2677072646323282 1105 86-87 -0.010340513550971764 1105 88-89 -0.04235177135628021 1105 90-91 0.09030012931523501 1105 92-93 0.5135175517441937 1105 94-95 0.29258129337698335 1105 96-97 -0.32237159250632175 1105 98-99 0.08543400352328945 1105 100-101 0.5092937082606959 1106 1 -0.4733747187103887 1106 2 -0.37966706703257813 1106 3 -0.4347794142896326 1106 4 -0.7670217923802269 1106 5 -0.5206934887629515 1106 6 -0.5513300472855676 1106 7 -0.514162454846705 1106 8 -0.4128838145034379 1106 9 -0.897422278375025 1106 10-11 -0.8486092604991313 1106 12-13 -0.9045865698578623 1106 14-15 -0.9798013098684635 1106 16-17 -0.49899180663059894 1106 18-19 -0.952814262428717 1106 20-21 -0.2299250303184337 1106 22-23 -0.6521194932862855 1106 24-25 -0.5320452767047286 1106 26-27 -0.9450899475129191 1106 28-29 -0.7214709935964407 1106 30-31 -0.7455024996716446 1106 32-33 -0.307759512354032 1106 34-35 -0.44710024653866043 1106 36-37 -0.8740988306869895 1106 38-39 -0.6363731327893447 1106 40-41 -0.9470495824571188 1106 42-43 -1.009360665336935 1106 44-45 -1.0635947780976167 1106 46-47 -0.5910981775738975 1106 48-49 -0.7276696073949296 1106 50-51 -1.2104925720789979 1106 52-53 -0.5071990321268274 1106 54-55 -0.8888133004822194 1106 56-57 -0.6796941592720174 1106 58-59 -0.9956658333931401 1106 60-61 -1.0288926503949583 1106 62-63 -1.1410677617577107 1106 64-65 -0.834937082736694 1106 66-67 -0.3953873402362902 1106 68-69 -0.8712406123293945 1106 70-71 -0.7916999524049189 1106 72-73 -0.764236426404441 1106 74-75 -0.46314085214823564 1106 76-77 -0.045301374957958274 1106 78-79 0.0825971694744645 1106 80-81 -0.07607662091405132 1106 82-83 -0.36987471948059536 1106 84-85 -0.6224340844462191 1106 86-87 -0.5327953355226995 1106 88-89 -0.6206305332099191 1106 90-91 -0.6733427406078292 1106 92-93 -1.3270621584007358 1106 94-95 -1.711086625749875 1106 96-97 -1.3211191323166638 1106 98-99 -1.4808569250864778 1106 100-101 -1.7935332733102491 1107 1 -0.1276742356186027 1107 2 0.043134865334572225 1107 3 0.008698846579932251 1107 4 0.15993472935890196 1107 5 0.01283308128535765 1107 6 0.08422550826998787 1107 7 0.1787844050566747 1107 8 0.16875869757385686 1107 9 0.1350414897409209 1107 10-11 0.22153566703710226 1107 12-13 0.13821536250928546 1107 14-15 0.138266322981778 1107 16-17 0.09511447356263858 1107 18-19 0.39588138974519893 1107 20-21 0.061475935865139775 1107 22-23 0.09986118304221492 1107 24-25 0.3582928875464759 1107 26-27 0.07626270949191394 1107 28-29 -0.26794442354812986 1107 30-31 0.024352572792125216 1107 32-33 -0.35819429496272903 1107 34-35 0.1491316375193108 1107 36-37 0.029765903612528177 1107 38-39 -0.08120405066374303 1107 40-41 -0.2397065872880333 1107 42-43 -0.30085824987799725 1107 44-45 -0.16543052688988524 1107 46-47 -0.7270402065594013 1107 48-49 -0.6297952112596619 1107 50-51 -0.6846954706297197 1107 52-53 -0.4880685973442169 1107 54-55 -0.36002102995082197 1107 56-57 -0.8515782172430306 1107 58-59 -0.7493856401564258 1107 60-61 -0.2943998967717718 1107 62-63 -0.4410677617577079 1107 64-65 -0.19918829046616082 1107 66-67 -0.21084627743436357 1107 68-69 -0.6511439939719068 1107 70-71 -0.24812507317786725 1107 72-73 -0.37785961481023733 1107 74-75 -0.7380200792013767 1107 76-77 -0.7175719063589234 1107 78-79 -0.578852105887858 1107 80-81 -0.8902795194647766 1107 82-83 -0.5923867967752834 1107 84-85 -0.6150910892771364 1107 86-87 -0.9306697316579644 1107 88-89 -0.7728044462533958 1107 90-91 -0.5231978130715937 1107 92-93 -0.4953713371446966 1107 94-95 -0.3591059494213731 1107 96-97 -0.7973510163746376 1107 98-99 -0.7395042680816495 1107 100-101 -0.24222892548415942 1108 1 -0.09656312450749027 1108 2 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-1.0340030649912997 2316 88-89 -0.9069652027545771 2316 90-91 -0.7078786641354249 2316 92-93 -0.9025580983740085 2316 94-95 -1.0896969659698321 2316 96-97 -0.9324101180294804 2316 98-99 -0.9686768449137979 2316 100-101 -0.976697010590545 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 4.0 5 9.0 6 15.0 7 44.0 8 119.0 9 193.0 10 327.0 11 551.0 12 821.0 13 1132.0 14 1439.0 15 2115.0 16 2944.0 17 4236.0 18 3761.0 19 2289.0 20 1956.0 21 1879.0 22 1952.0 23 2167.0 24 2441.0 25 2840.0 26 3480.0 27 4185.0 28 5090.0 29 6181.0 30 7846.0 31 9746.0 32 12237.0 33 16348.0 34 22603.0 35 32759.0 36 50737.0 37 77240.0 38 63732.0 39 6212.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.87647098991646 20.039605763321422 15.152618760385131 20.931304486376984 2 16.149600006825338 21.32946185063319 39.50021471371978 23.02072342882169 3 20.029803970639676 25.32569653983864 30.336346909117793 24.30815258040389 4 13.28750878051139 17.343180777576496 37.218561503394184 32.150748938517935 5 13.456691793485993 38.54473047930593 33.583274071967004 14.415303655241074 6 31.632023342651816 37.15542713810785 16.793172388100025 14.419377131140315 7 28.55009939396697 34.11274887595235 19.842391598010416 17.494760132070265 8 27.305044208275152 35.00828994030674 18.98211477372587 18.704551077692237 9 27.295090592126403 16.78776899647642 19.969797884714374 35.9473425266828 10-11 25.427650008105086 27.340024059312178 26.499085689259477 20.733240243323255 12-13 26.54848883870803 24.773972817013966 27.47520739895966 21.202330945318344 14-15 23.160927222002613 25.87513615124946 26.50861272185899 24.45532390488893 16-17 22.107834633465195 29.439241705082885 26.879029437108787 21.57389422434313 18-19 21.940329493133426 27.32822191445009 30.277620573840192 20.453828018576292 20-21 22.588025515384025 26.366560399964733 30.595283123501627 20.450130961149615 22-23 22.335298943916822 26.26443458242269 30.632810955849838 20.767455517810653 24-25 22.43011119642806 26.632397690754484 30.37980263344993 20.557688479367517 26-27 22.069141054918678 26.831404690739387 30.46719696043273 20.632257293909205 28-29 21.75433956307884 27.119514133886202 30.351416801400376 20.774729501634578 30-31 21.75177956437288 26.845044947686635 30.414696087660076 20.988479400280408 32-33 21.76244415310368 26.975152930202402 30.218894238562584 21.043508678131335 34-35 22.167840719390497 26.8965193626273 30.16343837716242 20.77220154081978 36-37 22.47270576257497 26.523685340598526 30.080823363127823 20.92278553369868 38-39 22.003606052936174 26.987239464097307 30.16329618264601 20.845858300320508 40-41 21.99606974356641 27.160432385085503 29.961806552892096 20.881691318455996 42-43 22.005594799424877 27.201192343629337 29.540512520034756 21.25270033691103 44-45 22.170968998751533 27.320543410563914 29.37880903560835 21.129678555076204 46-47 22.44000102380052 27.784808506644747 28.781023288617895 20.994167180936834 48-49 22.66125569133552 27.82974197383052 28.40989560078605 21.099106734047908 50-51 22.494461523585805 27.916907212390264 28.407762683039888 21.180868580984043 52-53 22.760080880240935 28.201011856178777 27.920462075300527 21.118445188279757 54-55 22.375444713350074 28.220919088476272 28.041327414249594 21.362308783924057 56-57 22.45962386706519 28.255472355964063 28.02682357357571 21.258080203395036 58-59 22.624569506101565 27.795188706342728 28.09976936049438 21.480472427061322 60-61 22.495599079717092 27.758218132075957 28.67267106711297 21.073511721093986 62-63 22.087785206651294 28.117685869562127 28.736658599497765 21.057870324288814 64-65 22.02351328523367 27.96752846023246 28.423972857910705 21.584985396623164 66-67 22.113522414121622 28.008196091925914 28.16702736675663 21.71125412719584 68-69 22.143383262567863 27.743714291402068 28.27182472535129 21.841077720678783 70-71 22.170258026169478 28.09920058242874 28.10815883696261 21.62238255443917 72-73 22.254073884480846 27.694451554190486 28.09401928163126 21.95745527969741 74-75 22.131581117705775 28.0281033242234 27.915627461742567 21.924688096328254 76-77 22.098876378931323 27.777698780824217 28.055120282341434 22.06830455790303 78-79 22.214907104322428 27.683708205476766 27.945772699221628 22.155611990979178 80-81 22.109825356694945 27.971225517659136 27.694372794207567 22.224576331438353 82-83 21.86730369991184 27.844467195631772 27.88982737536615 22.398401729090235 84-85 21.644081052257377 27.766797013864203 28.0501955207963 22.53892641308212 86-87 21.703291234276843 27.67560311804135 27.87040960552397 22.75069604215783 88-89 21.57360983531031 27.445248001456076 27.56369603362616 23.41744612960746 90-91 21.799650445617843 27.427220018743398 27.860252196802815 22.912877338835948 92-93 21.53199924351334 27.209488504104506 27.968961295469185 23.289550956912965 94-95 21.18684075067329 27.380833885522037 27.830737335445395 23.601588028359274 96-97 21.07393830464322 27.459467453097137 28.070477290113782 23.396116952145857 98-99 21.25156947420659 27.94311073698101 27.407889816156448 23.397429972655956 100-101 21.11570782710596 27.904641766655907 27.175788121889926 23.803862284348206 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 165.0 1 110.0 2 75.0 3 111.0 4 101.0 5 94.0 6 180.5 7 384.0 8 609.5 9 718.5 10 661.5 11 591.5 12 594.5 13 626.0 14 675.5 15 684.5 16 718.5 17 761.0 18 813.5 19 878.5 20 918.0 21 1002.0 22 1111.5 23 1298.0 24 1576.5 25 1863.0 26 2191.5 27 2635.5 28 3106.0 29 3692.0 30 4367.5 31 4978.5 32 5689.5 33 6295.0 34 6893.5 35 7566.0 36 8301.5 37 8967.5 38 9492.5 39 9905.5 40 10057.0 41 10645.5 42 11456.0 43 12039.0 44 12546.0 45 13008.0 46 13052.5 47 13409.5 48 14685.5 49 14452.0 50 13386.5 51 12687.0 52 11990.0 53 11701.5 54 11379.5 55 10458.0 56 9110.5 57 8022.5 58 7231.0 59 6507.5 60 5442.5 61 4279.0 62 3434.0 63 2614.5 64 1826.0 65 1322.0 66 944.5 67 810.5 68 605.5 69 323.0 70 239.5 71 181.5 72 141.5 73 107.0 74 72.0 75 49.0 76 33.5 77 20.5 78 10.0 79 9.0 80 7.5 81 4.0 82 3.5 83 2.0 84 1.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.047492968481163494 2 0.0 3 0.0 4 0.0 5 0.0230355116585284 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003981446459498736 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.2658354923200744E-4 24-25 2.843890328213383E-4 26-27 5.687780656426766E-4 28-29 0.00327047387744539 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.015499202288762936 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.843890328213383E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.843890328213383E-4 84-85 0.0017063341969280298 86-87 0.0 88-89 0.0 90-91 0.0115177558292642 92-93 0.0015641396805173603 94-95 0.0 96-97 0.0 98-99 1.4219451641066914E-4 100-101 0.01464603519029892 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 351631.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.90090530155425 #Duplication Level Percentage of deduplicated Percentage of total 1 86.14140405457306 61.075035314162065 2 8.527707187544484 12.092443194869503 3 2.3535228835772513 5.006007092806548 4 0.9656856799470604 2.7387195577997425 5 0.5504281736832717 1.9512927908812552 6 0.31916453708116316 1.3577432771523572 7 0.21515096290749902 1.067807863264996 8 0.1560784749708922 0.8852884138817786 9 0.11100126554522442 0.7083081195097171 >10 0.6047964116828752 7.460220329463365 >50 0.0345634429922909 1.6490119743025462 >100 0.01888932349535026 2.256527704210495 >500 4.019004999010694E-4 0.22083709695203102 >1k 0.001205701499703208 1.5307572707436266 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2250 0.6398753238480112 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1828 0.5198631519974063 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1294 0.36799940847081175 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 775 0.22040150043653714 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 424 0.12058094991624743 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 422 0.12001217185060475 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 2.843890328213383E-4 0.0 0.0 0.0 0.0 8 2.843890328213383E-4 0.0 0.0 0.0 0.0 9 2.843890328213383E-4 0.0 0.0 0.0 0.0 10-11 2.843890328213383E-4 0.0 0.0 0.0 0.0 12-13 2.843890328213383E-4 0.0 0.0 0.0 0.0 14-15 2.843890328213383E-4 0.0 0.0 2.843890328213383E-4 0.0 16-17 2.843890328213383E-4 0.0 0.0 2.843890328213383E-4 0.0 18-19 2.843890328213383E-4 0.0 0.0 2.843890328213383E-4 0.0 20-21 2.843890328213383E-4 0.0 0.0 2.843890328213383E-4 0.0 22-23 2.843890328213383E-4 0.0 0.0 2.843890328213383E-4 0.0 24-25 2.843890328213383E-4 0.0 0.0 8.531670984640149E-4 0.0 26-27 2.843890328213383E-4 0.0 0.0 0.001137556131285353 0.0 28-29 2.843890328213383E-4 0.0 0.0 0.0027016958118027136 0.0 30-31 2.843890328213383E-4 0.0 0.0 0.00654094775489078 0.0 32-33 2.843890328213383E-4 0.0 0.0 0.009384838083104162 0.0 34-35 2.843890328213383E-4 0.0 0.0 0.012655311960549554 0.0 36-37 2.843890328213383E-4 0.0 0.0 0.017632120034922975 0.0 38-39 5.687780656426766E-4 0.0 0.0 0.02602159650315245 0.0 40-41 5.687780656426766E-4 0.0 0.0 0.04308493847243275 0.0 42-43 5.687780656426766E-4 0.0 0.0 0.0797711237063854 0.0 44-45 5.687780656426766E-4 0.0 0.0 0.11460878022699933 0.0 46-47 5.687780656426766E-4 0.0 0.0 0.1939533203841527 0.0 48-49 5.687780656426766E-4 0.0 0.0 0.27344005505771674 0.0 50-51 5.687780656426766E-4 0.0 0.0 0.3389917271230352 0.0 52-53 5.687780656426766E-4 0.0 0.0 0.4285742724617568 0.0 54-55 5.687780656426766E-4 0.0 0.0 0.5160239000543183 0.0 56-57 5.687780656426766E-4 0.0 0.0 0.7190776694887537 0.0 58-59 5.687780656426766E-4 0.0 0.0 0.9171546308488159 0.0 60-61 5.687780656426766E-4 0.0 0.0 1.1550460568038654 0.0 62-63 5.687780656426766E-4 0.0 0.0 1.4030332934240723 0.0 64-65 5.687780656426766E-4 0.0 0.0 1.675335792350504 0.0 66-67 5.687780656426766E-4 0.0 0.0 2.058550014077257 0.0 68-69 5.687780656426766E-4 0.0 0.0 2.5552354598997242 0.0 70-71 5.687780656426766E-4 0.0 0.0 2.962764943932702 0.0 72-73 5.687780656426766E-4 0.0 0.0 3.37242734571184 0.0 74-75 5.687780656426766E-4 0.0 0.0 3.8565996740901687 0.0 76-77 5.687780656426766E-4 0.0 0.0 4.397792003549175 0.0 78-79 5.687780656426766E-4 0.0 0.0 4.9974262792529665 0.0 80-81 5.687780656426766E-4 2.843890328213383E-4 0.0 5.783761955003968 0.0 82-83 5.687780656426766E-4 2.843890328213383E-4 0.0 6.518196632265074 0.0 84-85 5.687780656426766E-4 2.843890328213383E-4 0.0 7.275240237635476 0.0 86-87 5.687780656426766E-4 2.843890328213383E-4 0.0 8.062286885968529 0.0 88-89 5.687780656426766E-4 2.843890328213383E-4 0.0 9.313456435866007 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGGT 170 0.0 44.70769 4 AGGTAAG 160 0.0 44.53305 2 TAAGGTG 185 0.0 41.08274 5 CAGTCCG 60 8.277035E-5 39.58493 9 GGTAAGG 200 0.0 38.001537 3 GGTGTGC 390 0.0 35.321938 8 GGGCGTA 55 0.0025766902 34.546852 9 AAGGTAA 215 0.0 30.966719 1 AAGGTGT 250 0.0 30.396904 6 AGGTGTG 300 0.0 28.501152 7 CTTCGTA 35 0.008376105 27.15168 90-91 GGTATCA 2170 0.0 26.298267 1 GTATCAA 3015 0.0 25.867956 1 GGTGTAG 155 1.3916215E-6 24.545046 1 ACGAATC 100 2.0534571E-8 23.747583 72-73 GTACATG 1925 0.0 22.728077 1 AGGACGA 105 0.0021926973 22.61996 5 GAGTACT 2010 0.0 22.214827 12-13 TTGCGTA 130 6.2391337E-10 21.920845 60-61 GTGTGCA 370 0.0 21.825207 9 >>END_MODULE