FastQCFastQC Report
Thu 26 May 2016
SRR937356_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937356_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595346
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33920.5697527152277835No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24480.41118945957476827No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17310.2907552918806878No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9730.163434372616932No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6030.10128563893937308No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20900.041.1570661
GTATCAA34050.034.7531661
TATCAAC42450.027.4191532
TCAACGC42700.027.0383824
ATCAACG42750.027.004483
CAACGCA43250.026.694545
AACGCAG44000.026.1315386
GTACATG28100.023.8465181
GAGTACT27500.023.74993912-13
CCGACGC500.001654108623.74893694-95
CTACACT1852.5312147E-723.113894
ACGCAGA49750.023.1093627
CGCAGAG49700.023.0350788
TACATGG29450.022.9070242
GTATAGA1903.2655043E-722.5113221
AGTACTT28650.022.13344812-13
GTACTTT29650.021.86756514-15
ACATGGG28600.021.5944923
AGAGTAC44450.021.3722710-11
GCAGAGT53600.020.9158889
Produced by FastQC (version 0.11.5)