##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937356_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 595346 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.673040551208878 33.0 31.0 34.0 30.0 34.0 2 31.764496612054167 33.0 31.0 34.0 30.0 34.0 3 31.80338156299026 33.0 31.0 34.0 30.0 34.0 4 35.419127700530446 37.0 35.0 37.0 33.0 37.0 5 35.43079990459329 37.0 35.0 37.0 33.0 37.0 6 35.45163820702583 37.0 35.0 37.0 33.0 37.0 7 35.3219119637992 37.0 35.0 37.0 33.0 37.0 8 35.332594827209725 37.0 35.0 37.0 33.0 37.0 9 36.952321843096286 39.0 37.0 39.0 33.0 39.0 10-11 36.83338260440148 39.0 37.0 39.0 33.0 39.0 12-13 36.69404094425762 39.0 37.0 39.0 32.5 39.0 14-15 37.85040211910385 40.0 38.0 41.0 33.0 41.0 16-17 37.79027069972756 40.0 38.0 41.0 33.0 41.0 18-19 37.77197713598479 40.0 38.0 41.0 33.0 41.0 20-21 37.66918732972087 40.0 37.5 41.0 32.0 41.0 22-23 37.511074232463145 40.0 37.0 41.0 32.0 41.0 24-25 37.43543166494778 40.0 37.0 41.0 32.0 41.0 26-27 37.419451041915124 40.0 37.0 41.0 32.0 41.0 28-29 37.32314318060422 40.0 37.0 41.0 32.0 41.0 30-31 37.085468786218435 39.0 36.5 41.0 31.0 41.0 32-33 36.834065400624176 39.0 36.0 41.0 30.5 41.0 34-35 36.88096165927041 39.0 36.0 41.0 31.0 41.0 36-37 36.77161096236475 39.0 36.0 41.0 30.0 41.0 38-39 36.644244691322356 39.0 36.0 41.0 30.0 41.0 40-41 36.41264995481619 39.0 35.0 41.0 30.0 41.0 42-43 36.131836780628404 39.0 35.0 41.0 30.0 41.0 44-45 35.87230702818193 39.0 35.0 41.0 28.5 41.0 46-47 35.50526164616878 39.0 35.0 40.5 26.5 41.0 48-49 35.13587980770846 39.0 35.0 40.0 25.5 41.0 50-51 34.121713255820985 38.0 34.0 39.5 23.5 40.5 52-53 34.218815613105654 38.0 33.5 39.5 24.0 40.5 54-55 34.586894007854255 38.0 34.0 40.0 23.5 41.0 56-57 34.360381190097854 38.0 34.0 40.0 23.0 41.0 58-59 34.074474339291775 38.0 34.0 40.0 22.0 41.0 60-61 33.75732935133519 38.0 33.0 40.0 21.5 41.0 62-63 33.429795446681425 37.0 33.0 40.0 20.0 41.0 64-65 32.99109257473805 36.5 32.5 39.5 18.0 41.0 66-67 32.62784918350002 36.0 32.0 39.0 17.0 41.0 68-69 32.15677605963591 35.5 31.5 39.0 12.5 40.0 70-71 31.66153631669651 35.0 31.0 38.5 8.0 40.0 72-73 31.20086386739812 35.0 31.0 37.5 7.0 40.0 74-75 30.70851656011798 35.0 30.0 37.0 3.5 39.0 76-77 30.230285413860177 34.5 30.0 36.5 2.0 39.0 78-79 29.71203048311402 34.0 29.0 36.0 2.0 38.5 80-81 29.216726071897686 34.0 29.0 35.5 2.0 37.0 82-83 28.736981519990056 34.0 28.5 35.0 2.0 37.0 84-85 28.217369395276023 34.0 27.5 35.0 2.0 36.5 86-87 27.761896443412738 33.5 26.5 35.0 2.0 36.0 88-89 27.29466646286361 33.0 26.0 35.0 2.0 35.5 90-91 26.78809633389659 33.0 25.0 35.0 2.0 35.0 92-93 26.270273924742924 32.5 24.5 35.0 2.0 35.0 94-95 25.70698551766536 32.0 22.5 35.0 2.0 35.0 96-97 24.979527031339757 32.0 19.0 34.0 2.0 35.0 98-99 24.08184148377582 31.5 3.5 34.0 2.0 35.0 100-101 21.67455647640196 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -8.275475239543084E-4 1101 2 -0.01883840096279954 1101 3 -0.0633463218661241 1101 4 -0.004615892067988625 1101 5 -0.035753268631793844 1101 6 -0.07778464772910354 1101 7 0.03326984766100338 1101 8 -0.17180223134131012 1101 9 -0.178849343806327 1101 10-11 -0.18666237935264718 1101 12-13 0.01936631943240741 1101 14-15 0.015895826204712193 1101 16-17 -0.07449588246365124 1101 18-19 0.05271472748251682 1101 20-21 0.19676259056436862 1101 22-23 0.09235071265477757 1101 24-25 0.04830897495550346 1101 26-27 0.04220566539737547 1101 28-29 0.09172498063048096 1101 30-31 0.14920109116741997 1101 32-33 -0.16936413184550503 1101 34-35 0.047666550886333425 1101 36-37 0.05326239449794201 1101 38-39 -0.014783417479591776 1101 40-41 -0.08713497750184018 1101 42-43 0.033513578577284875 1101 44-45 0.0755296837276731 1101 46-47 -0.041743150794843586 1101 48-49 -0.09629404098326688 1101 50-51 0.053886554364574124 1101 52-53 0.04544786291788938 1101 54-55 0.20115930137063742 1101 56-57 0.17187732860811167 1101 58-59 0.1460939082668915 1101 60-61 0.11651891109801937 1101 62-63 0.15367939044253376 1101 64-65 0.2305409520901378 1101 66-67 0.1388895308524596 1101 68-69 0.07592732205479535 1101 70-71 0.2292436164693079 1101 72-73 0.2006125396142444 1101 74-75 0.14324836597930712 1101 76-77 0.4678689101365485 1101 78-79 0.392388101652724 1101 80-81 0.26349331261522835 1101 82-83 0.1624922272701106 1101 84-85 0.20134817918179948 1101 86-87 0.26125298946079667 1101 88-89 0.30963177881536197 1101 90-91 0.22147628993976198 1101 92-93 0.18534932038165053 1101 94-95 0.22336025470664467 1101 96-97 0.29844802363761147 1101 98-99 0.14333289860583776 1101 100-101 0.6723199372603048 1102 1 -0.010876888361210035 1102 2 0.04090641439453435 1102 3 -0.013960191267095468 1102 4 -0.05062525911755955 1102 5 0.0982398699080278 1102 6 0.07578774453055104 1102 7 0.08518084141630311 1102 8 -0.09217652784889907 1102 9 -0.05049185402142342 1102 10-11 0.05696330486599521 1102 12-13 0.17227039390623133 1102 14-15 0.14706776745827455 1102 16-17 0.20404518992860687 1102 18-19 0.06531061446116837 1102 20-21 0.021207506145280774 1102 22-23 0.10280817545640986 1102 24-25 0.13484865963671666 1102 26-27 0.06937733681823488 1102 28-29 0.16450367926589848 1102 30-31 -0.06087956953722795 1102 32-33 0.004731446752906265 1102 34-35 0.2781523461218569 1102 36-37 0.34695360951605636 1102 38-39 0.5323992399870647 1102 40-41 0.492365446520985 1102 42-43 0.49245323242047334 1102 44-45 0.6422144998559602 1102 46-47 0.632072958217563 1102 48-49 0.5084612785757585 1102 50-51 0.5489929455448959 1102 52-53 0.5531414678828028 1102 54-55 0.48418826983876073 1102 56-57 0.6333182352459943 1102 58-59 0.7238423470919599 1102 60-61 1.006708083097088 1102 62-63 0.5954408197849119 1102 64-65 0.706136029570672 1102 66-67 0.5377090127736679 1102 68-69 0.5263253252927882 1102 70-71 0.5833783208097607 1102 72-73 0.6522165986690602 1102 74-75 0.43412320215230693 1102 76-77 0.5469785585830813 1102 78-79 0.48251704313757315 1102 80-81 0.4365803914834743 1102 82-83 0.5678064860011283 1102 84-85 0.6201680465782999 1102 86-87 0.5661186513218759 1102 88-89 0.6927966273235704 1102 90-91 0.7121270685998518 1102 92-93 0.9551656414054683 1102 94-95 0.9053335027214473 1102 96-97 0.9533392232367142 1102 98-99 0.8059297492727104 1102 100-101 0.8791018931619341 1103 1 -0.0964190245557397 1103 2 -0.11041994566369695 1103 3 -0.03655077195400125 1103 4 -0.1325583210682808 1103 5 0.04516255282170789 1103 6 0.01886277014116189 1103 7 -0.0611359483610201 1103 8 0.16031578896783572 1103 9 -0.08560400153854175 1103 10-11 -0.0774475836874089 1103 12-13 -0.1490844748056901 1103 14-15 -0.12369468738616973 1103 16-17 -0.10759584366172703 1103 18-19 -0.3261533015360314 1103 20-21 -0.2270604078030587 1103 22-23 -0.3935628683781687 1103 24-25 -0.1532090451133783 1103 26-27 -0.16578434701203548 1103 28-29 -0.21023145310052627 1103 30-31 -0.10294803074388881 1103 32-33 -0.05854260026277558 1103 34-35 0.19535020830981153 1103 36-37 0.19040706560060272 1103 38-39 0.03990146406643191 1103 40-41 0.04794586383914634 1103 42-43 0.0348371085190351 1103 44-45 -0.15233279889856277 1103 46-47 0.03854469219071177 1103 48-49 0.07944142361269257 1103 50-51 0.045910236751019795 1103 52-53 -0.07612438373740105 1103 54-55 0.19652078992287159 1103 56-57 0.20453190861635306 1103 58-59 0.06641392200973684 1103 60-61 0.24576594959841458 1103 62-63 0.21475998191355927 1103 64-65 0.3621022909606495 1103 66-67 0.4444735838633278 1103 68-69 0.434711452429255 1103 70-71 0.43031145015682526 1103 72-73 0.25113522432280533 1103 74-75 0.2524664652137183 1103 76-77 0.38718283690302613 1103 78-79 0.4722020326237448 1103 80-81 0.17344282206524042 1103 82-83 0.14150405164516755 1103 84-85 0.17191698171605907 1103 86-87 0.22041516827879448 1103 88-89 0.3721770527292989 1103 90-91 0.6173715151410697 1103 92-93 0.5340254300505336 1103 94-95 0.04106300075347491 1103 96-97 -0.20473721769712583 1103 98-99 0.16672967648466042 1103 100-101 0.5797344078543389 1104 1 0.03350471043215464 1104 2 -0.12371148404689336 1104 3 -0.10183726517358238 1104 4 -0.09545306203359871 1104 5 -0.19630512757814955 1104 6 -0.08768007849609916 1104 7 -0.09394811785971768 1104 8 0.046247303221989 1104 9 -0.012130868601616385 1104 10-11 -0.06407427683335953 1104 12-13 -0.01045019432906713 1104 14-15 -0.1878398494346314 1104 16-17 -0.3197953078089455 1104 18-19 -0.07634034079273277 1104 20-21 -0.2522024988823617 1104 22-23 -0.3335511759764671 1104 24-25 -0.4003417626860539 1104 26-27 -0.12138749475541744 1104 28-29 -0.2923779195274605 1104 30-31 -0.09246893605305928 1104 32-33 -0.12922091624357535 1104 34-35 -0.09426783990127063 1104 36-37 -0.3081651687644822 1104 38-39 -0.3438859535775407 1104 40-41 -0.42929116484150853 1104 42-43 -0.3124267323859655 1104 44-45 -0.1398839164336394 1104 46-47 -0.0508047441454309 1104 48-49 0.028838980938751035 1104 50-51 -0.2864017251438966 1104 52-53 -0.2570740927003996 1104 54-55 -0.38921859290633165 1104 56-57 -0.4151169080420871 1104 58-59 -0.2406624291775188 1104 60-61 -0.15976061976866873 1104 62-63 0.2644453446717847 1104 64-65 0.19734078070189298 1104 66-67 -0.05063940351592322 1104 68-69 -0.05415481548146772 1104 70-71 -0.25907705877797227 1104 72-73 -0.014761153970457741 1104 74-75 0.04149499702410253 1104 76-77 -0.16144590094431877 1104 78-79 0.19229582644576837 1104 80-81 0.21208944125722695 1104 82-83 0.23272290128518947 1104 84-85 0.2944453215908709 1104 86-87 0.24496480516802777 1104 88-89 0.2122002220948147 1104 90-91 0.40675062314987187 1104 92-93 0.3823463854979785 1104 94-95 0.30889685113773524 1104 96-97 0.18201883610750613 1104 98-99 0.5357468684280668 1104 100-101 1.2336990525385048 1105 1 -0.15213285020540468 1105 2 -0.2779330132684237 1105 3 -0.34149240614451415 1105 4 -0.3772475083017284 1105 5 -0.23901433476103762 1105 6 -0.20383216175344643 1105 7 -0.012224187451572277 1105 8 -0.31427718888144796 1105 9 -0.12487265897498645 1105 10-11 -0.3470776749683324 1105 12-13 -0.28534350869606584 1105 14-15 -0.5197849017744218 1105 16-17 -0.5804775487543452 1105 18-19 -0.5180720522612816 1105 20-21 -0.2925842267640917 1105 22-23 -0.1755173830742507 1105 24-25 -0.2803158663443668 1105 26-27 -0.1799171782851019 1105 28-29 -0.3110433460876294 1105 30-31 -0.23303409635506256 1105 32-33 -0.37036844028583005 1105 34-35 -0.41298280993202496 1105 36-37 -0.3087943404462834 1105 38-39 -0.28509563491879675 1105 40-41 -0.26250443884425323 1105 42-43 -0.18924965855099174 1105 44-45 -0.40417021690414145 1105 46-47 -0.4299255147135739 1105 48-49 -0.20592716172212988 1105 50-51 -0.19531924590351935 1105 52-53 -0.21576228494122773 1105 54-55 0.053467514253455306 1105 56-57 -0.045503620665648725 1105 58-59 0.14065056397758013 1105 60-61 0.22440796176832833 1105 62-63 0.21264126326244082 1105 64-65 0.07020022921923896 1105 66-67 0.0808779944488407 1105 68-69 -0.41875144652546936 1105 70-71 -0.6702285949295081 1105 72-73 -0.25849223322785164 1105 74-75 -0.3196996996969119 1105 76-77 -0.17971847513794614 1105 78-79 -0.262781467447315 1105 80-81 -0.11449169453443275 1105 82-83 -0.3846624496527973 1105 84-85 -0.14117096270962293 1105 86-87 -0.37714237286020236 1105 88-89 -0.48456562269734604 1105 90-91 -0.46148654113992293 1105 92-93 -0.08194736169050643 1105 94-95 -0.4797493355347662 1105 96-97 -0.32824547678838556 1105 98-99 -0.4081041181860776 1105 100-101 0.017329342747743226 1106 1 -0.2392316677729731 1106 2 -0.23285277678193594 1106 3 -0.24541699273768458 1106 4 0.11555121147067382 1106 5 0.04084097284920318 1106 6 -0.1449383584106272 1106 7 -0.5873375409366375 1106 8 -0.3575487704959599 1106 9 -0.7266407510802466 1106 10-11 -0.9236662907222453 1106 12-13 -0.42939550682906713 1106 14-15 -0.520797581806427 1106 16-17 -0.3215981924243323 1106 18-19 -0.624475690685891 1106 20-21 -0.6444634497370672 1106 22-23 -0.6732947657200583 1106 24-25 -0.5904960094809297 1106 26-27 -0.4454893911229405 1106 28-29 -0.1866337288223363 1106 30-31 -0.4830188158607527 1106 32-33 -0.2799537554529721 1106 34-35 -0.2749152090252025 1106 36-37 -0.5180509860721756 1106 38-39 -0.29847162398352367 1106 40-41 -0.6479493512731338 1106 42-43 -0.5373032014030557 1106 44-45 -0.2270984597028658 1106 46-47 -0.24751114104714134 1106 48-49 -0.07816555965953853 1106 50-51 -0.39995006916098674 1106 52-53 -0.18752948799954794 1106 54-55 -0.20822900957300305 1106 56-57 -0.02228838240106512 1106 58-59 -0.10731574114333142 1106 60-61 -0.057997799255851135 1106 62-63 -0.11769107359744169 1106 64-65 0.12026746339419958 1106 66-67 0.4305054282148433 1106 68-69 0.440077623086907 1106 70-71 0.3625258391921129 1106 72-73 0.09932137808082686 1106 74-75 0.31791226465230693 1106 76-77 0.1179344409360219 1106 78-79 0.18506759460816014 1106 80-81 -0.10882402028123295 1106 82-83 -0.12902255811652097 1106 84-85 -0.7211462916569928 1106 86-87 -0.41767041117812553 1106 88-89 -0.06243085429407458 1106 90-91 0.4881725649233779 1106 92-93 0.49458429949370597 1106 94-95 0.1425807453685053 1106 96-97 0.2279256202955402 1106 98-99 0.12373765368447565 1106 100-101 0.3875118196325218 1107 1 0.045557798189467036 1107 2 0.023107463828392127 1107 3 -0.04771122689261276 1107 4 0.0788728077148022 1107 5 -0.14928153301934088 1107 6 -0.07710165066414731 1107 7 0.13144913746711495 1107 8 -0.08322181920487992 1107 9 0.015601766525385585 1107 10-11 0.12714246808057084 1107 12-13 0.08579393712633276 1107 14-15 0.02110290968418127 1107 16-17 -0.03480975345719628 1107 18-19 0.2688956047953255 1107 20-21 0.13467754204697258 1107 22-23 0.14037894320013322 1107 24-25 -0.028005179081873166 1107 26-27 0.05618864584889138 1107 28-29 -0.03036997442092826 1107 30-31 -0.09991747343116941 1107 32-33 -0.11132937898583606 1107 34-35 -0.14032839124350716 1107 36-37 0.13685108258979284 1107 38-39 0.2456845893380759 1107 40-41 0.36755185911419375 1107 42-43 0.6349294130335679 1107 44-45 0.8244759607783578 1107 46-47 0.761213734246283 1107 48-49 0.5480998452700447 1107 50-51 0.29622437332727714 1107 52-53 0.36200469627275567 1107 54-55 0.5162978390185486 1107 56-57 0.8203245870825029 1107 58-59 0.9068788011101887 1107 60-61 1.3180291960493165 1107 62-63 0.8831479448884778 1107 64-65 1.03056125740828 1107 66-67 1.2623120229918356 1107 68-69 1.0528407906338515 1107 70-71 1.169702885847041 1107 72-73 1.0049661844188584 1107 74-75 1.0195105260959707 1107 76-77 1.133192656898462 1107 78-79 1.4707281726598005 1107 80-81 1.447147223850223 1107 82-83 1.064694431320099 1107 84-85 0.6219251578735232 1107 86-87 0.7621867264392428 1107 88-89 1.2455531172434853 1107 90-91 1.149873892681594 1107 92-93 1.304264647204974 1107 94-95 1.7315900616830575 1107 96-97 1.9873336308589202 1107 98-99 2.0466488185905796 1107 100-101 2.589408093106698 1108 1 -0.1938148591898674 1108 2 -0.20834025975196369 1108 3 -0.15637684457692202 1108 4 0.057138404931166065 1108 5 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0.20855347321806406 2316 3 0.3603796541601234 2316 4 0.1661898976020595 2316 5 0.6897061644550391 2316 6 0.8212740047280604 2316 7 0.5349822583334358 2316 8 0.29825928151134207 2316 9 0.4548430536642769 2316 10-11 0.4708507028908926 2316 12-13 0.5096227983716659 2316 14-15 1.0724890856205818 2316 16-17 0.6851878477216502 2316 18-19 1.0778723362301648 2316 20-21 0.5884573988030795 2316 22-23 0.4074534094624198 2316 24-25 1.0727538764679778 2316 26-27 1.077135494825967 2316 28-29 0.7756082305754788 2316 30-31 1.1796394300334114 2316 32-33 0.9882302659886335 2316 34-35 0.8764804912485218 2316 36-37 0.8736147301687041 2316 38-39 1.0906350708157433 2316 40-41 1.6155728316648208 2316 42-43 1.0228444947648256 2316 44-45 0.8889271447679405 2316 46-47 0.7749212836791344 2316 48-49 0.7984596684426464 2316 50-51 0.6916614728098196 2316 52-53 0.731294645212131 2316 54-55 0.4121804338576567 2316 56-57 0.44035988402229265 2316 58-59 0.7658024157909793 2316 60-61 0.7684164343207911 2316 62-63 0.5824899825156962 2316 64-65 0.9954527964233932 2316 66-67 0.45444902994842096 2316 68-69 0.8013843635066138 2316 70-71 0.9915390120935719 2316 72-73 0.2499543616574691 2316 74-75 0.37527283718880255 2316 76-77 0.8058906453155821 2316 78-79 1.1351844969804255 2316 80-81 1.1237261622005192 2316 82-83 0.985456228379828 2316 84-85 0.9740806700218414 2316 86-87 0.8114653666503422 2316 88-89 1.100227391600086 2316 90-91 1.8188708427518456 2316 92-93 1.5075647397309346 2316 94-95 1.2035838247480655 2316 96-97 1.9297105199305769 2316 98-99 1.9345981691954215 2316 100-101 1.474333215617925 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2718.0 3 664.0 4 511.0 5 523.0 6 606.0 7 691.0 8 898.0 9 1083.0 10 1439.0 11 1820.0 12 2290.0 13 2971.0 14 3379.0 15 4277.0 16 5399.0 17 7174.0 18 6260.0 19 4425.0 20 4359.0 21 4514.0 22 5132.0 23 5333.0 24 5990.0 25 6976.0 26 7807.0 27 9104.0 28 10569.0 29 12730.0 30 15536.0 31 18726.0 32 23794.0 33 30784.0 34 41399.0 35 57665.0 36 82197.0 37 110508.0 38 84521.0 39 10574.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.19443508724651 18.893249343124705 15.132048777201373 22.780266792427405 2 14.850684949958918 19.896550330688722 40.85554762321691 24.397217096135453 3 18.84568178718805 24.594039002560773 30.33633521392819 26.22394399632299 4 12.167832403325802 16.11882095025779 37.58094769844183 34.13239894797458 5 13.252054559261047 37.117101038350086 34.49891652621636 15.131927876172501 6 31.43191559234403 35.985752418804786 17.75625857844169 14.82607341040949 7 28.078808608387096 32.948350687865975 20.737361336357957 18.235479367388972 8 26.5986252513244 34.09171277788817 19.894452171745915 19.415209799041516 9 26.1991638224394 16.365574270112628 20.45671526689151 36.978546640556466 10-11 24.684541922707087 26.460924961983366 27.375513414020098 21.479019701289452 12-13 25.793696569412084 23.84024101599897 28.155777976304247 22.210284438284695 14-15 22.50211109361806 24.924712045146673 26.89352258386072 25.679654277374546 16-17 21.58922988708019 28.591504112719413 27.297612020979123 22.521653979221274 18-19 21.455356451281567 26.75346280911023 30.309135484826953 21.482045254781255 20-21 22.219387926912223 25.691596117969546 30.432249678405697 21.65676627671253 22-23 21.71256113884516 25.573842255465667 30.54568260836624 22.167913997322938 24-25 21.61611808073351 25.90331272891653 30.502982912335963 21.977586278013998 26-27 21.360758294796447 26.070129977277762 30.519530266714824 22.04958146121097 28-29 21.142884566486543 26.299681573173338 30.610315277920442 21.947118582419673 30-31 21.298477579619334 26.00541881294655 30.49496764372568 22.201135963708435 32-33 21.147240090256965 26.153302125079986 30.333664500050517 22.365793284612533 34-35 21.681291116793798 26.20503307867901 30.09877713718916 22.014898667338038 36-37 21.644337959587762 25.90924167703547 30.193676348519773 22.252744014856994 38-39 21.335030491603444 26.23018719251634 30.26699936356565 22.167782952314568 40-41 21.628649742569188 26.06087085357928 30.2647041986982 22.04577520515333 42-43 21.38085588752049 26.219710854043843 29.84106485453044 22.55836840390523 44-45 21.42655668110108 26.392157424067776 29.753862812176102 22.427423082655046 46-47 21.65020675579072 26.599126161027858 29.306864999541105 22.443802083640314 48-49 21.755711692169854 26.779356733616037 28.881222401487417 22.58370917272669 50-51 21.62369037534293 26.976962731799986 28.944789036961687 22.454557855895395 52-53 21.87338394651421 26.937369136515777 28.708521644799234 22.48072527217078 54-55 21.548130895422098 26.878830287628393 28.864222355827934 22.708816461121582 56-57 21.644134986358086 27.160334814118897 28.633628059329077 22.561902140193943 58-59 21.851200937316932 26.833558908887678 28.695819781966076 22.619420371829317 60-61 21.698769879513 26.746766883119616 29.136727281909273 22.417735955458106 62-63 21.585460703968323 26.709331923690748 29.243489942828667 22.46171742951226 64-65 21.352760364412543 26.823493566219792 29.081545049928287 22.742201019439374 66-67 21.38304749207119 27.037250267660546 28.75212950056899 22.827572739699278 68-69 21.45329418736196 26.69953334590314 29.068324758929055 22.77884770780585 70-71 21.49230163192609 26.809613111972286 28.835969038183773 22.862116217917855 72-73 21.463798968174004 26.741280026532188 28.868989748238832 22.925931257054977 74-75 21.314752773615716 27.045320628293407 28.662687924039126 22.97723867405175 76-77 21.407981869642594 26.793462430188526 28.701544481505113 23.097011218663763 78-79 21.453194858459472 26.90132865502196 28.596599188647463 23.048877297871105 80-81 21.454534740811827 26.951277000521912 28.438810040911157 23.155378217755104 82-83 21.455279160129024 26.73098808746187 28.658694671344588 23.155038081064518 84-85 21.354762898816176 26.72261989737512 28.489010249020215 23.433606954788488 86-87 21.47898972909769 26.682458989207174 28.572234302170436 23.2663169795247 88-89 21.37098776811697 26.65171668093481 28.33965088079491 23.637644670153314 90-91 21.242438069196822 26.623973572598747 28.655445347844612 23.478143010359826 92-93 21.31048322606387 26.3571424963846 28.729164106285154 23.603210171266376 94-95 21.11214366402524 26.34575595499641 28.60578420429733 23.93631617668103 96-97 21.045205587854483 26.27662135837328 28.74702616970658 23.931146884065658 98-99 21.11741579182206 26.477610432469294 28.480759031174497 23.92421474453415 100-101 21.264862189646845 26.003508175361635 28.80617667804074 23.92545295695078 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 977.0 1 576.0 2 195.0 3 214.5 4 182.0 5 155.5 6 273.5 7 562.5 8 937.5 9 1147.5 10 1008.0 11 844.5 12 867.5 13 919.0 14 982.5 15 1054.0 16 1072.0 17 1107.5 18 1200.5 19 1286.0 20 1388.5 21 1578.0 22 1790.0 23 2035.0 24 2408.5 25 2965.0 26 3610.0 27 4346.0 28 5188.0 29 6033.5 30 6900.0 31 7939.0 32 9256.0 33 10345.5 34 11430.5 35 12445.5 36 13635.0 37 14949.5 38 15887.0 39 16529.0 40 17037.5 41 18106.0 42 19092.0 43 19960.5 44 20650.0 45 21217.5 46 21555.0 47 22429.0 48 24389.0 49 24488.0 50 22799.0 51 21562.0 52 20865.5 53 20479.5 54 20056.0 55 18512.0 56 16474.0 57 14542.5 58 12967.5 59 11336.0 60 9306.5 61 7780.5 62 6348.5 63 4739.5 64 3455.5 65 2645.0 66 1884.0 67 1415.0 68 1048.0 69 641.5 70 469.0 71 363.0 72 273.5 73 201.0 74 152.0 75 117.5 76 78.0 77 53.5 78 35.5 79 26.0 80 18.0 81 10.5 82 8.5 83 7.0 84 4.5 85 2.5 86 2.0 87 1.5 88 1.0 89 0.5 90 0.5 91 1.0 92 1.0 93 0.5 94 0.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27311848908029956 2 0.24086833538816085 3 0.23263782741464625 4 0.24254803089295973 5 0.23902067033288207 6 0.2622004682991067 7 0.2608567118952676 8 0.2506105693159944 9 0.2440597568472787 10-11 0.2564895035827905 12-13 0.2564055188075506 14-15 0.24515155892539797 16-17 0.25489379285323155 18-19 0.2459074219025575 20-21 0.24019645718624127 22-23 0.23700503572712336 24-25 0.23171399488700686 26-27 0.2409523201634008 28-29 0.2501066606645547 30-31 0.2501066606645547 32-33 0.24960275201311505 34-35 0.2577492752113897 36-37 0.2380128530300027 38-39 0.2371730052776033 40-41 0.23515737067184458 42-43 0.23927262465860188 44-45 0.2537180059998723 46-47 0.25690942745899026 48-49 0.2627043769505464 50-51 0.26354422470294586 52-53 0.28437244896245206 54-55 0.26186452919814696 56-57 0.2359132336490041 58-59 0.22004011112865457 60-61 0.21810846129813585 62-63 0.22541513674401104 64-65 0.21735259832097636 66-67 0.22004011112865457 68-69 0.22339950213825238 70-71 0.22239168483537303 72-73 0.2268428779230901 74-75 0.2277667104507295 76-77 0.23935660943384182 78-79 0.23507338589660465 80-81 0.23112610146032728 82-83 0.22675889314785017 84-85 0.2247432585420915 86-87 0.23288978174036612 88-89 0.22936242118028843 90-91 0.2380128530300027 92-93 0.22827061910216917 94-95 0.2265909235973703 96-97 0.23767691392904294 98-99 0.24036442673672115 100-101 0.21920026337625517 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 595346.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.60009546814643 #Duplication Level Percentage of deduplicated Percentage of total 1 85.20978197072125 59.30608959982142 2 9.142082217095641 12.725795901750011 3 2.4319036742785314 5.077821836973657 4 1.0755569982903879 2.994354790497761 5 0.5558473556934882 1.9343514510991766 6 0.34782301537679594 1.4525109045746138 7 0.22559043927411798 1.0990781277125794 8 0.1667034904634651 0.9282063080904328 9 0.1308877947237631 0.8198822707550159 >10 0.6580204445697084 8.144972906245629 >50 0.037684891703188554 1.8889715423412612 >100 0.016668291184874867 2.0022906462598735 >500 7.247083123793343E-4 0.352742108978939 >1k 7.247083123793343E-4 1.2729316048996888 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3392 0.5697527152277835 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2448 0.41118945957476827 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1731 0.2907552918806878 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 973 0.163434372616932 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 603 0.10128563893937308 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.67969550479889E-4 0.0 12-13 0.0 0.0 0.0 1.67969550479889E-4 0.0 14-15 0.0 0.0 0.0 1.67969550479889E-4 0.0 16-17 0.0 0.0 0.0 1.67969550479889E-4 0.0 18-19 0.0 0.0 0.0 1.67969550479889E-4 0.0 20-21 0.0 0.0 0.0 1.67969550479889E-4 0.0 22-23 0.0 0.0 0.0 2.519543257198335E-4 0.0 24-25 0.0 0.0 0.0 5.03908651439667E-4 0.0 26-27 0.0 0.0 0.0 0.001175786853359223 0.0 28-29 8.39847752399445E-5 0.0 0.0 0.0014277411790790564 0.0 30-31 1.67969550479889E-4 0.0 0.0 0.004031269211517336 0.0 32-33 1.67969550479889E-4 0.0 0.0 0.006886751569675449 0.0 34-35 1.67969550479889E-4 0.0 0.0 0.01007817302879334 0.0 36-37 1.67969550479889E-4 0.0 0.0 0.013773503139350898 0.0 38-39 1.67969550479889E-4 0.0 0.0 0.02233995021382524 0.0 40-41 1.67969550479889E-4 0.0 0.0 0.03544157515125658 0.0 42-43 1.67969550479889E-4 0.0 0.0 0.06072099249847987 0.0 44-45 1.67969550479889E-4 0.0 0.0 0.08675627282286266 0.0 46-47 2.519543257198335E-4 0.0 0.0 0.1411784071783467 0.0 48-49 5.03908651439667E-4 1.67969550479889E-4 0.0 0.20324315608066568 0.0 50-51 5.03908651439667E-4 1.67969550479889E-4 0.0 0.2528781582474729 0.0 52-53 5.03908651439667E-4 1.67969550479889E-4 0.0 0.31301125731927315 0.0 54-55 5.03908651439667E-4 1.67969550479889E-4 0.0 0.38196275779126765 0.0 56-57 5.03908651439667E-4 1.67969550479889E-4 0.0 0.5221333476667349 0.0 58-59 5.03908651439667E-4 1.67969550479889E-4 0.0 0.6612121354640831 0.0 60-61 5.03908651439667E-4 1.67969550479889E-4 0.0 0.8242265842048153 0.0 62-63 5.03908651439667E-4 1.67969550479889E-4 0.0 0.9937078606390234 0.0 64-65 5.03908651439667E-4 1.67969550479889E-4 0.0 1.1765427163363826 0.0 66-67 5.03908651439667E-4 1.67969550479889E-4 0.0 1.4291689202581357 0.0 68-69 5.03908651439667E-4 1.67969550479889E-4 0.0 1.7530981983586016 0.0 70-71 5.03908651439667E-4 1.67969550479889E-4 0.0 2.0319276521552174 0.0 72-73 5.03908651439667E-4 1.67969550479889E-4 0.0 2.3121848471309123 0.0 74-75 5.03908651439667E-4 1.67969550479889E-4 0.0 2.634938338378019 0.0 76-77 5.03908651439667E-4 1.67969550479889E-4 0.0 2.9864986075324262 0.0 78-79 5.03908651439667E-4 1.67969550479889E-4 0.0 3.3947485999737967 0.0 80-81 6.71878201919556E-4 1.67969550479889E-4 0.0 3.8950459060781464 8.39847752399445E-5 82-83 6.71878201919556E-4 1.67969550479889E-4 0.0 4.395343212182496 1.67969550479889E-4 84-85 6.71878201919556E-4 1.67969550479889E-4 0.0 4.897068259465923 1.67969550479889E-4 86-87 6.71878201919556E-4 1.67969550479889E-4 0.0 5.415506277022102 1.67969550479889E-4 88-89 6.71878201919556E-4 1.67969550479889E-4 0.0 6.171285269406361 1.67969550479889E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2090 0.0 41.157066 1 GTATCAA 3405 0.0 34.753166 1 TATCAAC 4245 0.0 27.419153 2 TCAACGC 4270 0.0 27.038382 4 ATCAACG 4275 0.0 27.00448 3 CAACGCA 4325 0.0 26.69454 5 AACGCAG 4400 0.0 26.131538 6 GTACATG 2810 0.0 23.846518 1 GAGTACT 2750 0.0 23.749939 12-13 CCGACGC 50 0.0016541086 23.748936 94-95 CTACACT 185 2.5312147E-7 23.11389 4 ACGCAGA 4975 0.0 23.109362 7 CGCAGAG 4970 0.0 23.035078 8 TACATGG 2945 0.0 22.907024 2 GTATAGA 190 3.2655043E-7 22.511322 1 AGTACTT 2865 0.0 22.133448 12-13 GTACTTT 2965 0.0 21.867565 14-15 ACATGGG 2860 0.0 21.594492 3 AGAGTAC 4445 0.0 21.37227 10-11 GCAGAGT 5360 0.0 20.915888 9 >>END_MODULE