FastQCFastQC Report
Thu 26 May 2016
SRR937356_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937356_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595346
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38420.6453390129437335No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29500.49551017391567254No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20910.35122433005344794No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10380.17435239339812478No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6800.11421929432632452No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30850.027.4159951
CGCCGAA350.00838656727.14625442-43
GTATCAA42950.026.9939141
GTAAGGT2550.026.0784074
GTACATG28650.023.5506481
GTTGTGC1852.5332884E-723.1119698
GTATAGG1652.4195542E-623.0380231
TAAGGTG3007.2759576E-1222.1666475
GTATTAG1302.9325474E-421.9304261
TATCAAC53150.021.895562
ATCAACG53200.021.696413
TCAACGC53550.021.5546044
CAACGCA53750.021.47445
GAGTACT28700.021.27097512-13
AGTACTT28600.021.1792412-13
TACATGG30550.020.9901622
AACGCAG54850.020.9553786
GGTATAG1150.003713979220.6590981
TAGTAAC1150.003721218320.6521572
GTAAGTA2108.5934516E-720.3571242
Produced by FastQC (version 0.11.5)