##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937356_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 595346 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16767560376655 33.0 31.0 34.0 31.0 34.0 2 32.366642255091996 34.0 31.0 34.0 31.0 34.0 3 32.420523527494936 34.0 31.0 34.0 31.0 34.0 4 36.07441051086259 37.0 35.0 37.0 35.0 37.0 5 35.995589119604396 37.0 35.0 37.0 35.0 37.0 6 36.041202930732716 37.0 35.0 37.0 35.0 37.0 7 35.977502158408726 37.0 35.0 37.0 35.0 37.0 8 35.94207570051701 37.0 35.0 37.0 35.0 37.0 9 37.64226684986546 39.0 37.0 39.0 35.0 39.0 10-11 37.554344028514514 39.0 37.0 39.0 35.0 39.0 12-13 37.451551702707334 39.0 37.0 39.0 34.5 39.0 14-15 38.615765454038495 40.0 38.0 41.0 34.0 41.0 16-17 38.577499470895916 40.0 38.0 41.0 34.0 41.0 18-19 38.58896087317291 40.0 38.0 41.0 34.0 41.0 20-21 38.49505833582488 40.0 38.0 41.0 34.0 41.0 22-23 38.33410739301179 40.0 38.0 41.0 34.0 41.0 24-25 38.26441430697443 40.0 38.0 41.0 34.0 41.0 26-27 38.18635297793216 40.0 38.0 41.0 34.0 41.0 28-29 38.03801570851236 40.0 37.0 41.0 33.0 41.0 30-31 38.1291408693432 40.0 37.5 41.0 34.0 41.0 32-33 38.06175652477719 40.0 37.0 41.0 33.5 41.0 34-35 37.92988615023869 40.0 37.0 41.0 33.0 41.0 36-37 37.745243102330406 40.0 37.0 41.0 33.0 41.0 38-39 37.61314596889876 40.0 37.0 41.0 33.0 41.0 40-41 37.42426420938412 40.0 36.5 41.0 33.0 41.0 42-43 37.20518404423646 40.0 36.0 41.0 32.0 41.0 44-45 36.88555058738952 39.5 36.0 41.0 31.0 41.0 46-47 36.48083887352901 39.0 35.5 41.0 30.5 41.0 48-49 36.108722490786874 39.0 35.0 41.0 29.5 41.0 50-51 35.856802598824885 39.0 35.0 40.5 28.5 41.0 52-53 35.58915823739473 39.0 35.0 40.0 28.0 41.0 54-55 35.34755587507097 38.5 35.0 40.0 27.5 41.0 56-57 35.065508964534914 38.0 34.0 40.0 27.0 41.0 58-59 34.768801671632964 38.0 34.0 40.0 26.0 41.0 60-61 34.41614288161842 38.0 34.0 40.0 26.0 41.0 62-63 34.03365689867741 37.0 33.5 40.0 25.5 41.0 64-65 33.643621524290076 36.5 33.0 39.5 24.5 41.0 66-67 33.222085140405746 36.0 32.5 39.0 23.0 40.5 68-69 32.75843627067285 35.5 32.0 39.0 22.0 40.0 70-71 32.3259348345332 35.0 32.0 38.0 20.5 40.0 72-73 31.84707464230884 35.0 31.0 37.5 19.5 39.5 74-75 31.35498012920218 35.0 31.0 37.0 18.0 39.0 76-77 30.09802954920332 33.5 29.5 35.5 17.5 38.0 78-79 30.709465588078196 34.0 30.5 36.0 17.5 38.0 80-81 30.512522969836027 34.0 31.0 35.5 13.5 37.0 82-83 30.177995652948034 34.0 30.0 35.0 9.0 37.0 84-85 29.82953442199998 34.0 30.0 35.0 7.0 36.5 86-87 29.400883687805074 34.0 29.5 35.0 2.0 36.0 88-89 28.942350330732047 34.0 29.0 35.0 2.0 35.5 90-91 28.427549693791512 34.0 29.0 35.0 2.0 35.0 92-93 28.051748898959595 33.0 28.5 35.0 2.0 35.0 94-95 27.599955320099575 33.0 27.0 35.0 2.0 35.0 96-97 27.105762867307412 33.0 26.5 35.0 2.0 35.0 98-99 26.333348170643625 33.0 25.0 35.0 2.0 35.0 100-101 23.905543163135388 30.5 12.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.14547816710839356 1101 2 -0.03322287291451431 1101 3 -0.03693320372797615 1101 4 0.05660728032390239 1101 5 -0.04725642911743222 1101 6 -0.019709480021894876 1101 7 -0.08133811117392042 1101 8 -0.019882752474700283 1101 9 -0.020902643291215384 1101 10-11 -0.04673082139778728 1101 12-13 -0.18000855219273149 1101 14-15 -0.31754683966118336 1101 16-17 -0.13999177351298187 1101 18-19 -0.14590039492546936 1101 20-21 -0.08824080930935452 1101 22-23 -0.05452979493218635 1101 24-25 0.05927242775761954 1101 26-27 -0.06505196254086343 1101 28-29 -0.1420112029548335 1101 30-31 -0.1821572628131065 1101 32-33 -0.010984792973737001 1101 34-35 0.035340289233140254 1101 36-37 0.02471436700322016 1101 38-39 0.06772949457042898 1101 40-41 0.06549321811565534 1101 42-43 0.10330243260797545 1101 44-45 0.10984289591151963 1101 46-47 0.010069564267539022 1101 48-49 -0.24102981770863607 1101 50-51 -0.2025451936161815 1101 52-53 -0.16461581033299666 1101 54-55 -0.3743325404449749 1101 56-57 -0.12951981123251244 1101 58-59 0.0012148413064707597 1101 60-61 -0.13616347992769562 1101 62-63 -0.17337197625848688 1101 64-65 -0.21017567662708103 1101 66-67 0.003380082828464026 1101 68-69 -0.07955281549064352 1101 70-71 -0.04475530440647901 1101 72-73 0.06276486403455905 1101 74-75 0.08802595694552195 1101 76-77 0.07911382586596716 1101 78-79 -0.011511716629900803 1101 80-81 -0.03008232062276406 1101 82-83 -0.00661734008798831 1101 84-85 -0.05220346882279969 1101 86-87 -0.13815254408325828 1101 88-89 -0.07784303459692765 1101 90-91 -0.10880370051038923 1101 92-93 -0.18178999375513172 1101 94-95 0.01890139366799204 1101 96-97 -0.09634846371544015 1101 98-99 0.018553342788969474 1101 100-101 0.5929727019340412 1102 1 -0.08898387214270542 1102 2 -0.024127583422568932 1102 3 -0.01015423525985426 1102 4 -0.04656229025662384 1102 5 0.04746448677186521 1102 6 0.03823709310276513 1102 7 -0.006473027525785824 1102 8 -0.08713566281314655 1102 9 -0.05148432550538473 1102 10-11 -0.1300961170573416 1102 12-13 0.021707776540594637 1102 14-15 0.04572941120613194 1102 16-17 0.009919085325968524 1102 18-19 -2.2301847030092858E-4 1102 20-21 0.055577130710695144 1102 22-23 0.034735681900748716 1102 24-25 -0.023835081301051275 1102 26-27 0.03614831114659012 1102 28-29 -0.02861593659141448 1102 30-31 -1.628136572975336E-4 1102 32-33 0.07605938249461985 1102 34-35 -0.012236998562705992 1102 36-37 -0.013532009529043876 1102 38-39 -0.03011039826743911 1102 40-41 0.17073568207371181 1102 42-43 0.10459425667705347 1102 44-45 0.11495478011473637 1102 46-47 0.2108882174168798 1102 48-49 0.41474893874807606 1102 50-51 0.3266844340146875 1102 52-53 0.376778453794671 1102 54-55 0.38658026504418785 1102 56-57 0.34856832769278867 1102 58-59 0.33095136894504407 1102 60-61 0.45141361513051237 1102 62-63 0.3769277307802881 1102 64-65 0.22506061972632097 1102 66-67 0.3768637194023583 1102 68-69 0.6048142340814806 1102 70-71 0.5996977061555455 1102 72-73 0.5213094057044323 1102 74-75 0.2565596089384279 1102 76-77 0.2845846839339643 1102 78-79 0.3422408853283194 1102 80-81 0.2937766725157722 1102 82-83 0.3275160150889498 1102 84-85 0.2306139700793679 1102 86-87 0.3551575322408489 1102 88-89 0.5709595827803007 1102 90-91 0.44779467278388196 1102 92-93 0.3696468653921947 1102 94-95 0.4916198617891858 1102 96-97 0.3580093344496973 1102 98-99 0.5176474758550391 1102 100-101 1.1106130149399824 1103 1 -0.10558426573735602 1103 2 0.0493573467548174 1103 3 -0.02851030362670315 1103 4 -0.15457908542565235 1103 5 0.0033266208097089134 1103 6 -0.030729586144552457 1103 7 -0.14818192923495843 1103 8 -0.22134941705911615 1103 9 -0.2168493441067767 1103 10-11 -0.11261990796691634 1103 12-13 -0.11371102434364389 1103 14-15 -0.24237412305816264 1103 16-17 -0.3636935609240979 1103 18-19 -0.2682114397783266 1103 20-21 -0.33718787171557807 1103 22-23 -0.3521954929523261 1103 24-25 -0.22089997050145627 1103 26-27 -0.11229180046175458 1103 28-29 -0.2482714064787288 1103 30-31 -0.2790650619993471 1103 32-33 -0.26482324003193725 1103 34-35 -0.3306510277857697 1103 36-37 -0.22277912496550556 1103 38-39 -0.16519255441129133 1103 40-41 -0.05667036742216425 1103 42-43 0.035655546912664704 1103 44-45 -0.08267590139709569 1103 46-47 0.025957029084530348 1103 48-49 -0.1022784106884913 1103 50-51 -0.07614823434137463 1103 52-53 -0.12925891073867746 1103 54-55 -0.18082147108564328 1103 56-57 -0.11617158981408693 1103 58-59 -0.22335458350008963 1103 60-61 -0.18435564674472715 1103 62-63 -0.1088887085360497 1103 64-65 -0.321316152797543 1103 66-67 -0.09892785066288212 1103 68-69 -0.1274996172152214 1103 70-71 -0.1301588744235218 1103 72-73 -0.05375832762171129 1103 74-75 0.1771478442325467 1103 76-77 0.23481854924910905 1103 78-79 -0.007569056980411659 1103 80-81 0.007359731770641531 1103 82-83 -0.02861941112553623 1103 84-85 0.0678935840331043 1103 86-87 0.03525000002539258 1103 88-89 0.09828104986392461 1103 90-91 -0.023256575396413126 1103 92-93 0.29393718188542906 1103 94-95 0.023148882655096514 1103 96-97 0.008353055806246346 1103 98-99 -0.0883669739212003 1103 100-101 0.5715736824333746 1104 1 -0.1926603427309388 1104 2 -0.07297122344267848 1104 3 -0.01940511509896936 1104 4 0.039492259768515225 1104 5 -0.03926251171984063 1104 6 -0.0896538068469539 1104 7 -0.10943934225680607 1104 8 -0.15517864923848634 1104 9 -0.1655114748266513 1104 10-11 -0.1194532060467779 1104 12-13 -0.10283973477162078 1104 14-15 -0.18262340427903467 1104 16-17 -0.3179183958203353 1104 18-19 -0.2756660803104225 1104 20-21 -0.3890432815567806 1104 22-23 -0.4000682396678812 1104 24-25 -0.18207414615775974 1104 26-27 -0.34112355913998726 1104 28-29 -0.4013346394571684 1104 30-31 -0.2610539649896282 1104 32-33 -0.27374579598703264 1104 34-35 -0.16777998498805147 1104 36-37 -0.2395406822288919 1104 38-39 -0.4272100714700571 1104 40-41 -0.397213035875005 1104 42-43 -0.41419345071359714 1104 44-45 -0.32682186644132116 1104 46-47 -0.05299752918945444 1104 48-49 -0.019303977290135776 1104 50-51 -0.13519339403961084 1104 52-53 -0.05887388406355143 1104 54-55 0.0941531659994368 1104 56-57 0.005886703761163403 1104 58-59 0.0621353810114087 1104 60-61 0.08296213197314017 1104 62-63 0.16001596607440405 1104 64-65 0.059374345216511415 1104 66-67 -0.007050936203476965 1104 68-69 0.05889920140174354 1104 70-71 0.44676909881517446 1104 72-73 0.4145189331051391 1104 74-75 0.4912504083351088 1104 76-77 0.3584157548238558 1104 78-79 0.18988417341278208 1104 80-81 0.19213640102256235 1104 82-83 0.17372328004621096 1104 84-85 0.30388571467463876 1104 86-87 0.16513113706739446 1104 88-89 0.29682371048266276 1104 90-91 0.03499929822380565 1104 92-93 0.09457145061089989 1104 94-95 0.13726780045183418 1104 96-97 0.23076574470610467 1104 98-99 0.31789257389425885 1104 100-101 0.5511296061918642 1105 1 -0.19835024315768024 1105 2 -0.20745743029731045 1105 3 -0.17431204632168829 1105 4 -0.05915851651383974 1105 5 -0.36958220388164165 1105 6 -0.3541673082025625 1105 7 -0.29670606241825936 1105 8 -0.24452102805454956 1105 9 -0.398428095901167 1105 10-11 -0.3171134168648848 1105 12-13 -0.2732016452651109 1105 14-15 -0.3155944319869022 1105 16-17 -0.36903403575176696 1105 18-19 -0.4381952592869638 1105 20-21 -0.4257183675739711 1105 22-23 -0.5911836972307043 1105 24-25 -0.5841239692602116 1105 26-27 -0.4179805274443211 1105 28-29 -0.48613831965558774 1105 30-31 -0.4503259799195334 1105 32-33 -0.3547455710657559 1105 34-35 -0.35242819595205077 1105 36-37 -0.2038534261469067 1105 38-39 -0.482963193012651 1105 40-41 -0.49540941038717534 1105 42-43 -0.3786817244123952 1105 44-45 -0.3901645613629583 1105 46-47 -0.7041560257426909 1105 48-49 -0.4189244986211804 1105 50-51 -0.8504043033294622 1105 52-53 -0.6233617018408424 1105 54-55 -0.45320720043986995 1105 56-57 -0.4816681587936955 1105 58-59 -0.5042308333811221 1105 60-61 -0.5686234110597681 1105 62-63 -0.6008304066140724 1105 64-65 -0.20544310381146147 1105 66-67 -0.3567009404587438 1105 68-69 -0.3860589146090092 1105 70-71 -0.42163523669442426 1105 72-73 -0.4262603583320477 1105 74-75 -0.26879810171339713 1105 76-77 -0.2312434894406472 1105 78-79 -0.11801557856592026 1105 80-81 -0.48643209662488474 1105 82-83 -0.5150426620092041 1105 84-85 -0.3874242693984975 1105 86-87 -0.5132823472671468 1105 88-89 -0.09929761327024167 1105 90-91 -0.1732527291071726 1105 92-93 -0.12787949223477924 1105 94-95 0.1304570929832316 1105 96-97 -0.2972893853227063 1105 98-99 -0.37577148490138157 1105 100-101 -0.3963163215172223 1106 1 -0.3118009677309388 1106 2 -0.18318089224609935 1106 3 -0.08889963967161663 1106 4 -0.11864409172721224 1106 5 -0.23447485146343183 1106 6 -0.25553588483840883 1106 7 -0.1602873657610786 1106 8 -0.17185533193079294 1106 9 -0.0875367152112645 1106 10-11 -0.2913782861749823 1106 12-13 -0.334059686694701 1106 14-15 -0.3184824454115116 1106 16-17 -0.17018201761521112 1106 18-19 -0.3627804633232401 1106 20-21 -0.15209750164225255 1106 22-23 -0.14974271883454549 1106 24-25 -0.13267009968340204 1106 26-27 -0.5922754020887027 1106 28-29 -0.3991811939443508 1106 30-31 -0.36127264821612215 1106 32-33 -0.2038923638289134 1106 34-35 -0.12879260518035807 1106 36-37 -0.4512043440878699 1106 38-39 -0.21540451591449994 1106 40-41 -0.3062174429262896 1106 42-43 -0.6877311109700059 1106 44-45 -0.4719707713558492 1106 46-47 -0.5635143561125346 1106 48-49 -0.5648351605166297 1106 50-51 -0.48998606230883723 1106 52-53 -0.35767696899944923 1106 54-55 -0.45702037833816433 1106 56-57 -0.5235019848072113 1106 58-59 -0.3774930244373067 1106 60-61 -0.2492527451636093 1106 62-63 -0.030413721425595952 1106 64-65 0.04505142854984712 1106 66-67 -0.1215508026564649 1106 68-69 0.0765858333461864 1106 70-71 -0.051244389432689275 1106 72-73 0.03320049026325833 1106 74-75 -0.1587896557674533 1106 76-77 -0.037267338124859606 1106 78-79 -9.461551128602252E-4 1106 80-81 -0.12374400763128435 1106 82-83 -0.32267929741105306 1106 84-85 -0.0346893387441618 1106 86-87 0.42502196238790546 1106 88-89 0.565720612192063 1106 90-91 0.4385115845485892 1106 92-93 0.5514023800980787 1106 94-95 0.30930138752448144 1106 96-97 0.31953277194969587 1106 98-99 0.35467642806092314 1106 100-101 0.4165643017046854 1107 1 -0.10580588263704271 1107 2 -0.11325351548553897 1107 3 -0.12320311678429618 1107 4 -0.12773116625773184 1107 5 0.0194680769403206 1107 6 -0.1905325837820726 1107 7 -0.05958680824934248 1107 8 0.131025756684231 1107 9 -0.12772707612676015 1107 10-11 -0.1719992515505666 1107 12-13 -0.1601031872815568 1107 14-15 -0.12369719746550345 1107 16-17 -0.14951373709877913 1107 18-19 -0.11642537969648004 1107 20-21 -0.08536114600844513 1107 22-23 0.12663363826639795 1107 24-25 0.09591429732363821 1107 26-27 -0.19023424891968688 1107 28-29 -0.04355656072838343 1107 30-31 0.207127989988102 1107 32-33 0.09385833306075853 1107 34-35 0.003400946756265455 1107 36-37 0.013506446698514196 1107 38-39 -0.07118503234642048 1107 40-41 0.09890469615822184 1107 42-43 0.4730853502976018 1107 44-45 0.4719021012967346 1107 46-47 0.2477202389578892 1107 48-49 0.5867887758096586 1107 50-51 0.8103871404493788 1107 52-53 0.5903451480780149 1107 54-55 0.7933601601008036 1107 56-57 0.7466023652514764 1107 58-59 0.43661624052748493 1107 60-61 0.6656258493199587 1107 62-63 0.4974572806283888 1107 64-65 0.5405950024935109 1107 66-67 0.6942777624843828 1107 68-69 0.5479445848133224 1107 70-71 0.7038263998865588 1107 72-73 1.0867005269416588 1107 74-75 1.069166623575267 1107 76-77 0.9764412152788928 1107 78-79 0.7991519596171877 1107 80-81 0.9125905360893718 1107 82-83 0.8626979399715786 1107 84-85 0.7736476624295463 1107 86-87 0.8302403822822733 1107 88-89 1.006631703741359 1107 90-91 0.7635189202293411 1107 92-93 0.5865586300980787 1107 94-95 0.7642465533108656 1107 96-97 0.945215907219648 1107 98-99 1.3545709776501234 1107 100-101 2.0318536943103176 1108 1 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68-69 0.3117378064301306 2316 70-71 0.0734847392169442 2316 72-73 0.4552536950990209 2316 74-75 0.14430112890407898 2316 76-77 0.19749258432039696 2316 78-79 0.17157751048933179 2316 80-81 0.4933365694672567 2316 82-83 0.360864555234933 2316 84-85 0.42884282366459914 2316 86-87 0.2942837666762266 2316 88-89 1.204919973505934 2316 90-91 1.1146635006069836 2316 92-93 1.3694053454265465 2316 94-95 1.1513477492945547 2316 96-97 0.7534472906540763 2316 98-99 1.4357453719477853 2316 100-101 1.1444526456827866 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 0.0 4 3.0 5 11.0 6 31.0 7 70.0 8 174.0 9 338.0 10 570.0 11 863.0 12 1278.0 13 1917.0 14 2377.0 15 3372.0 16 4702.0 17 6725.0 18 6142.0 19 3858.0 20 3331.0 21 3487.0 22 3580.0 23 3861.0 24 4414.0 25 5184.0 26 6383.0 27 7636.0 28 9390.0 29 11556.0 30 14298.0 31 17829.0 32 22559.0 33 29802.0 34 40884.0 35 57761.0 36 87310.0 37 127148.0 38 97542.0 39 8956.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.57397591333371 19.86225626787761 15.116349421362044 21.44741839742663 2 16.07518988957682 21.11209279981725 39.34132420474816 23.47139310585777 3 19.778246599456452 25.21273343568278 30.13726471665217 24.871755248208604 4 13.132195395618682 17.139276991866915 36.997813036452754 32.73071457606165 5 13.54579598747598 38.26880954620584 33.57599741994437 14.609397046373813 6 31.85441743120807 36.90190242312873 16.83021301898392 14.413467126679274 7 28.657956885575782 33.76473512881585 19.91262223984036 17.664685745768008 8 27.370302311596955 34.73391943508481 18.968633366143386 18.927144887174855 9 27.169242759672525 16.654852808282914 19.94352863712866 36.2323757949159 10-11 25.367077381247327 27.185100391454025 26.523886590033165 20.92393563726548 12-13 26.51895464910697 24.41145468060356 27.573796515217055 21.49579415507242 14-15 23.014515928552473 25.65919650085832 26.43857521508501 24.887712355504192 16-17 22.055829719188505 29.241315134392437 26.854551806848452 21.848303339570602 18-19 21.997460300396742 27.205692152126666 29.89152526430009 20.905322283176506 20-21 22.793719954446658 26.124388170912376 30.189503246851412 20.892388627789554 22-23 22.36228236157688 26.12862072817275 30.31136015668371 21.197736753566662 24-25 22.48226861008579 26.390439116978882 30.239483994507367 20.887808278427965 26-27 22.1086835656084 26.60046393423623 30.23215188555169 21.05870061460368 28-29 21.80503541351887 26.912930532727163 30.19148412585762 21.090549927896348 30-31 22.004859379501077 26.51595255512154 30.164350496814023 21.31483756856336 32-33 21.84116463367521 26.746127462013686 30.03404742788227 21.37866047642883 34-35 22.38546996200529 26.603101389780058 29.867001709930026 21.144426938284628 36-37 22.46218579143186 26.41552377193056 29.835893473532156 21.286396963105425 38-39 22.213889003202365 26.66360399871334 29.95683989777296 21.16566710031133 40-41 22.12147756467054 26.802128851446515 29.822816873255736 21.253576710627208 42-43 22.151280974380512 26.791432171356572 29.465350692986142 21.591936161276777 44-45 22.325691552216316 26.985506735164705 29.275185585512954 21.413616127106025 46-47 22.492466565660976 27.386175434117305 28.79921927752937 21.322138722692348 48-49 22.69614870692732 27.450446841371946 28.433489461161628 21.41991499053911 50-51 22.476929382241586 27.509213129843822 28.393404843569957 21.620452644344635 52-53 22.854692901270855 27.745546287369027 27.956851981872727 21.44290882948739 54-55 22.446190954503766 27.72219851985232 28.119026582861057 21.71258394278285 56-57 22.518417861210118 27.86102535332395 28.061161072720736 21.559395712745193 58-59 22.66690294383434 27.456806630094093 28.16639399609639 21.709896429975174 60-61 22.470966463199552 27.502830286925583 28.61487269587769 21.41133055399717 62-63 22.360946407635225 27.641321181296252 28.650986149230867 21.346746261837655 64-65 22.162406398967995 27.577576736889135 28.429434312147894 21.830582551994972 66-67 22.225310995622714 27.74563027214427 28.137587218189086 21.891471514043932 68-69 22.23429736657339 27.61360620546707 28.261632731218484 21.890463696741055 70-71 22.41192516620587 27.642664937700097 28.045623889301346 21.899786006792688 72-73 22.360694453309506 27.516183866188737 28.033446096891556 22.0896755836102 74-75 22.238160666234425 27.972725104393074 27.70808907761201 22.08102515176049 76-77 22.26469985521025 27.69885075233562 27.938123376994216 22.098326015459918 78-79 22.401846993177077 27.54515861364652 27.861949185851586 22.191045207324816 80-81 22.28949408368754 27.886916084861646 27.68879583367977 22.134793997771045 82-83 22.269897739794136 27.685926120024725 27.686094090139484 22.358082050041656 84-85 22.06628089595109 27.643467233284902 27.78632557592656 22.50392629483745 86-87 22.152832134590643 27.63821374461238 27.594289707161884 22.614664413635097 88-89 21.979151619394436 27.46570901626953 27.516183866188737 23.038955498147295 90-91 22.096036271671323 27.598858026946733 27.610533097144142 22.6945726042378 92-93 21.97486921020517 27.34470470356133 27.74944127204361 22.930984814189884 94-95 21.67453883959916 27.372233961427472 27.658538060220444 23.29468913875293 96-97 21.7318164563128 27.402384495738612 27.74033923130415 23.12545981664444 98-99 21.693807792120534 27.63590859081709 27.460800040312762 23.209483576749616 100-101 21.682096821069006 27.441315855344612 27.451143444158298 23.425443879428084 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 184.0 1 139.5 2 122.0 3 183.5 4 168.5 5 151.5 6 287.5 7 636.5 8 1033.5 9 1190.5 10 1053.0 11 897.5 12 916.5 13 951.5 14 1032.5 15 1142.0 16 1187.0 17 1187.5 18 1234.5 19 1322.5 20 1370.0 21 1534.5 22 1840.5 23 2117.5 24 2434.5 25 2917.0 26 3499.5 27 4226.0 28 5056.5 29 6016.0 30 7033.5 31 8015.5 32 9244.5 33 10221.5 34 11375.0 35 12562.0 36 13716.5 37 14949.0 38 15937.0 39 16581.0 40 16918.0 41 18085.0 42 19412.0 43 20363.5 44 21016.0 45 21688.5 46 21781.0 47 22445.0 48 24650.5 49 24655.0 50 22925.5 51 21725.5 52 20742.0 53 20172.5 54 19748.5 55 18347.0 56 16106.5 57 14116.5 58 12884.5 59 11459.0 60 9435.0 61 7595.5 62 6174.0 63 4724.0 64 3305.5 65 2487.0 66 1835.0 67 1486.0 68 1072.0 69 627.0 70 491.0 71 354.5 72 266.0 73 193.5 74 140.5 75 118.0 76 75.5 77 44.5 78 31.0 79 23.5 80 13.0 81 7.0 82 6.5 83 7.0 84 6.5 85 3.5 86 0.5 87 1.0 88 2.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05509401255740359 2 0.0 3 0.0 4 0.0 5 0.0016796955047988901 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.519543257198335E-4 12-13 0.007306675445875172 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010918020781192786 24-25 5.878934266796115E-4 26-27 5.03908651439667E-4 28-29 0.003779314885797503 30-31 4.1992387619972253E-4 32-33 0.0 34-35 0.0 36-37 1.67969550479889E-4 38-39 7.558629771595006E-4 40-41 2.519543257198335E-4 42-43 0.016125076846069345 44-45 8.39847752399445E-5 46-47 0.0 48-49 8.39847752399445E-5 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 8.39847752399445E-5 82-83 3.35939100959778E-4 84-85 1.67969550479889E-4 86-87 0.0 88-89 0.0 90-91 0.010162157804033286 92-93 0.0034433757848377246 94-95 0.0 96-97 0.0 98-99 1.67969550479889E-4 100-101 0.013941472689830788 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 595346.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.95947443902284 #Duplication Level Percentage of deduplicated Percentage of total 1 83.65517399838667 56.0150648503698 2 9.945526807013023 13.318944960336099 3 2.795879278527566 5.6163182125548055 4 1.1818924031744682 3.1655557664014426 5 0.6757953171538457 2.262544963248713 6 0.38174515397467734 1.5336872927872947 7 0.2584100912826617 1.2112102731418861 8 0.17177796468102716 0.9201729788197284 9 0.1427809271349942 0.8604482256788065 >10 0.7287299884560503 8.807937424151136 >50 0.037925416621009955 1.732890755888136 >100 0.022604523953174825 2.5914291954040656 >500 7.534841317698855E-4 0.2953176498877164 >1k 0.001004645509026514 1.6684774513303156 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3842 0.6453390129437335 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2950 0.49551017391567254 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2091 0.35122433005344794 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1038 0.17435239339812478 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 680 0.11421929432632452 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 8.39847752399445E-5 0.0 24-25 0.0 0.0 0.0 3.35939100959778E-4 0.0 26-27 0.0 0.0 0.0 0.001007817302879334 0.0 28-29 1.67969550479889E-4 0.0 0.0 0.0012597716285991675 0.0 30-31 1.67969550479889E-4 0.0 0.0 0.003863299661037447 0.0 32-33 1.67969550479889E-4 0.0 0.0 0.006634797243955616 0.0 34-35 1.67969550479889E-4 0.0 0.0 0.009742233927833563 0.0 36-37 1.67969550479889E-4 0.0 0.0 0.013437564038391121 0.0 38-39 1.67969550479889E-4 0.0 0.0 0.022087995888105404 0.0 40-41 1.67969550479889E-4 0.0 0.0 0.035357590376016636 0.0 42-43 2.519543257198335E-4 0.0 0.0 0.06063700772323993 0.0 44-45 3.35939100959778E-4 0.0 0.0 0.08625236417142301 0.0 46-47 4.1992387619972253E-4 0.0 0.0 0.1413463767288266 0.0 48-49 5.03908651439667E-4 0.0 0.0 0.20357909518162548 0.0 50-51 5.03908651439667E-4 0.0 0.0 0.25262620392175306 0.0 52-53 5.03908651439667E-4 0.0 0.0 0.3125073486678335 0.0 54-55 5.03908651439667E-4 0.0 0.0 0.38204674256650756 0.0 56-57 5.03908651439667E-4 0.0 0.0 0.5217134237905352 0.0 58-59 5.03908651439667E-4 0.0 0.0 0.6605402572621635 0.0 60-61 5.03908651439667E-4 0.0 0.0 0.8240586146543354 0.0 62-63 5.03908651439667E-4 0.0 0.0 0.9952195865933424 0.0 64-65 5.03908651439667E-4 0.0 0.0 1.1802380464469402 0.0 66-67 5.03908651439667E-4 0.0 0.0 1.4365595804792508 0.0 68-69 5.03908651439667E-4 0.0 0.0 1.762924417061675 0.0 70-71 5.03908651439667E-4 0.0 0.0 2.041585901307811 0.0 72-73 5.03908651439667E-4 0.0 0.0 2.323522791788305 0.0 74-75 5.03908651439667E-4 0.0 0.0 2.646696206911611 0.0 76-77 5.03908651439667E-4 0.0 0.0 3.0005240649974976 0.0 78-79 5.03908651439667E-4 0.0 0.0 3.4125533723246653 0.0 80-81 0.001007817302879334 0.0 0.0 3.915454206461453 0.0 82-83 0.001007817302879334 0.0 0.0 4.420622629529719 0.0 84-85 0.001007817302879334 0.0 0.0 4.930830139112381 0.0 86-87 0.001007817302879334 0.0 0.0 5.456490847339195 0.0 88-89 0.001007817302879334 0.0 0.0 6.225371464660887 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3085 0.0 27.415995 1 CGCCGAA 35 0.008386567 27.146254 42-43 GTATCAA 4295 0.0 26.993914 1 GTAAGGT 255 0.0 26.078407 4 GTACATG 2865 0.0 23.550648 1 GTTGTGC 185 2.5332884E-7 23.111969 8 GTATAGG 165 2.4195542E-6 23.038023 1 TAAGGTG 300 7.2759576E-12 22.166647 5 GTATTAG 130 2.9325474E-4 21.930426 1 TATCAAC 5315 0.0 21.89556 2 ATCAACG 5320 0.0 21.69641 3 TCAACGC 5355 0.0 21.554604 4 CAACGCA 5375 0.0 21.4744 5 GAGTACT 2870 0.0 21.270975 12-13 AGTACTT 2860 0.0 21.17924 12-13 TACATGG 3055 0.0 20.990162 2 AACGCAG 5485 0.0 20.955378 6 GGTATAG 115 0.0037139792 20.659098 1 TAGTAAC 115 0.0037212183 20.652157 2 GTAAGTA 210 8.5934516E-7 20.357124 2 >>END_MODULE