FastQCFastQC Report
Thu 26 May 2016
SRR937355_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937355_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences664589
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26050.3919715794272851No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19330.2908564541393252No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18260.27475627794020063No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15580.234430602974169No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT14620.219985585075889No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13800.20764713228777487No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12460.18748429480475903No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC12150.18281975777510612No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT9670.14550346153788282No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC7250.109089978919302No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGCA550.002573739534.5594029
TACATGG23500.029.7225192
ACATGGG24200.029.2554663
ACTGCGT650.00583079529.242578
GTACATG24050.029.058151
GGTATAC908.92641E-426.3975493
CATGGGA12700.024.3208394
CATGGGG11050.023.222044
ATGGGAG6500.022.6647035
GTATCAA43150.022.585971
GAGTACT22250.021.7742112-13
GTATTAC1100.00286182821.6094151
TATACAG3600.021.121235
CTGTTCG1150.003712942620.6605137
AGTACTT24500.020.55000912-13
AGAGTAC35750.019.93209610-11
GTACTTT24600.019.88498114-15
ATAGGGG1456.128709E-419.6616233
ATCAACG49400.019.5256393
TCAACGC49700.019.5048524
Produced by FastQC (version 0.11.5)