FastQCFastQC Report
Thu 26 May 2016
SRR937355_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937355_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences664589
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37710.5674183593168108No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29820.4486983684653222No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23040.34668042955871975No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16530.24872515193600855No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT10900.16401114072005404No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT10450.1572400385802353No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA8130.12233124532605867No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC8090.12172936958029698No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC7520.11315264020319325No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA7040.10593013125405326No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTA650.00583956229.2335939
GTATTAC1000.001651261223.7540821
AGTACTT28350.021.61584512-13
GAGTACT26750.021.48805812-13
GTACTAG1553.898059E-521.4553011
GTACATG22600.020.8110981
AGAGTAC39350.020.76439510-11
ACACCGT1150.003722811820.651066
ACATGGG22300.020.022853
TACATGG22700.019.8792822
TCGTTAC600.00476536819.79060252-53
GTACTTT28900.019.3934214-15
TACACTA2451.7528328E-719.388175
GCTAGAC1507.715264E-419.0004063
GAGTACA19800.018.7153381
AGTACAT18100.018.6329962
TACTTTT33000.018.27931814-15
CCTAGAC1300.00755892218.269623
GTATAGG1600.001188925217.8155611
GTATCAA67000.017.3723891
Produced by FastQC (version 0.11.5)