##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937355_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 664589 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.27847887942774 33.0 31.0 34.0 31.0 34.0 2 32.48324453158268 34.0 31.0 34.0 31.0 34.0 3 32.5542598508251 34.0 31.0 34.0 31.0 34.0 4 36.15172685674906 37.0 35.0 37.0 35.0 37.0 5 36.08838394857574 37.0 35.0 37.0 35.0 37.0 6 36.127749631727276 37.0 35.0 37.0 35.0 37.0 7 36.0673258209209 37.0 35.0 37.0 35.0 37.0 8 36.0438737324873 37.0 35.0 37.0 35.0 37.0 9 37.75164500164764 39.0 37.0 39.0 35.0 39.0 10-11 37.67590646249035 39.0 37.0 39.0 35.0 39.0 12-13 37.57087538313153 39.0 37.0 39.0 35.0 39.0 14-15 38.8060161994857 40.0 38.0 41.0 35.0 41.0 16-17 38.76913551081947 40.0 38.0 41.0 35.0 41.0 18-19 38.75488309315983 40.0 38.0 41.0 35.0 41.0 20-21 38.63841863166559 40.0 38.0 41.0 34.0 41.0 22-23 38.58050915678713 40.0 38.0 41.0 34.0 41.0 24-25 38.60849036020758 40.0 38.0 41.0 34.0 41.0 26-27 38.448241695243226 40.0 38.0 41.0 34.0 41.0 28-29 38.313401967230874 40.0 38.0 41.0 34.0 41.0 30-31 38.39984336183717 40.0 38.0 41.0 34.0 41.0 32-33 38.32435610580374 40.0 38.0 41.0 34.0 41.0 34-35 38.18620756587906 40.0 38.0 41.0 34.0 41.0 36-37 38.048471310840235 40.0 37.5 41.0 33.5 41.0 38-39 37.9326320477769 40.0 37.0 41.0 33.0 41.0 40-41 37.77741280701305 40.0 37.0 41.0 33.0 41.0 42-43 37.585136828927354 40.0 37.0 41.0 33.0 41.0 44-45 37.296761607549925 40.0 37.0 41.0 32.5 41.0 46-47 36.965158917767226 40.0 36.5 41.0 31.0 41.0 48-49 36.64592402221523 39.5 36.0 41.0 31.0 41.0 50-51 36.39859597435407 39.0 36.0 41.0 30.0 41.0 52-53 36.179018912440625 39.0 35.5 40.5 30.0 41.0 54-55 35.96252721606888 39.0 35.0 40.0 29.5 41.0 56-57 35.65436457720486 38.5 35.0 40.0 28.5 41.0 58-59 35.38312400596459 38.0 35.0 40.0 28.0 41.0 60-61 35.0108089360492 38.0 34.0 40.0 27.5 41.0 62-63 34.65107231687554 37.5 34.0 40.0 26.5 41.0 64-65 34.23148667823271 37.0 34.0 39.5 26.0 41.0 66-67 33.80407815958435 36.5 33.0 39.0 26.0 40.5 68-69 33.32505729104755 36.0 33.0 39.0 25.0 40.0 70-71 32.8691063198458 35.0 32.0 38.5 23.5 40.0 72-73 32.377524304494955 35.0 31.5 37.5 22.5 39.5 74-75 31.89058199879926 35.0 31.0 37.0 21.5 39.0 76-77 30.577457646756116 33.5 29.5 35.5 20.0 38.0 78-79 31.21892628376335 34.0 31.0 36.0 20.5 38.5 80-81 31.030152470173295 34.0 31.0 36.0 20.0 37.5 82-83 30.63391434405324 34.0 31.0 35.0 18.5 37.0 84-85 30.21723200353903 34.0 30.0 35.0 17.0 36.5 86-87 29.875596045074474 34.0 30.0 35.0 11.5 36.0 88-89 29.440239757203322 34.0 29.5 35.0 7.0 36.0 90-91 28.90831476295876 34.0 29.0 35.0 2.0 35.0 92-93 28.39956499430475 33.5 29.0 35.0 2.0 35.0 94-95 27.923709992190663 33.0 27.5 35.0 2.0 35.0 96-97 27.257165706925633 33.0 26.5 35.0 2.0 35.0 98-99 26.337177563877823 32.5 25.0 34.0 2.0 35.0 100-101 23.412173538833777 29.5 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08944056286121338 1101 2 0.14282242858131156 1101 3 0.13596571974593985 1101 4 0.05180091823310562 1101 5 0.04988707134970838 1101 6 0.11886037475996147 1101 7 0.1644778549188075 1101 8 0.11322000966274004 1101 9 0.1178447891668668 1101 10-11 0.11286910944749451 1101 12-13 0.2093897022573401 1101 14-15 0.23399976776913434 1101 16-17 0.12072224649297425 1101 18-19 0.09995755439587128 1101 20-21 0.12654809801411915 1101 22-23 0.17380801383273337 1101 24-25 0.17784471354961795 1101 26-27 0.062169966045708236 1101 28-29 0.06723285583002081 1101 30-31 0.11851713413587106 1101 32-33 0.2057317444059663 1101 34-35 0.31184766902051564 1101 36-37 0.30900182424432643 1101 38-39 0.39314422004774485 1101 40-41 0.2739309216103081 1101 42-43 0.331488328641214 1101 44-45 0.45556250541250165 1101 46-47 0.5085857518159784 1101 48-49 0.3983367580468169 1101 50-51 0.373671266578917 1101 52-53 0.39697750656701913 1101 54-55 0.4008157663118581 1101 56-57 0.2919306234014911 1101 58-59 0.37771647037912004 1101 60-61 0.5068361657213956 1101 62-63 0.5161487874237451 1101 64-65 0.5884344892559739 1101 66-67 0.4552755870095524 1101 68-69 0.30735111451567576 1101 70-71 0.3426238753482096 1101 72-73 0.25658693806185084 1101 74-75 0.24284785238947748 1101 76-77 0.45275195894217646 1101 78-79 0.6136874286752452 1101 80-81 0.47673560107258695 1101 82-83 0.1954399044830737 1101 84-85 0.26276988408015 1101 86-87 0.4136488578342288 1101 88-89 0.5956373477428052 1101 90-91 0.5600664588154807 1101 92-93 0.39009100237824157 1101 94-95 0.5443013929182463 1101 96-97 0.6495067294584942 1101 98-99 0.9032617534398995 1101 100-101 1.6194869390598932 1102 1 0.0728874773069208 1102 2 0.15264827493250976 1102 3 0.045137341323979285 1102 4 0.04072333682621121 1102 5 0.057441295453493524 1102 6 -0.011450903817113556 1102 7 3.28350166533653E-4 1102 8 0.06206682516234707 1102 9 0.11422589350509327 1102 10-11 0.12430693740420651 1102 12-13 0.06890267569706765 1102 14-15 0.07971383789185893 1102 16-17 0.08517011084554582 1102 18-19 0.1266976456380462 1102 20-21 0.10020537332098911 1102 22-23 0.0601280162181439 1102 24-25 0.02521062363767612 1102 26-27 -0.048756762118451036 1102 28-29 -0.10103970719639221 1102 30-31 -0.04982753905296278 1102 32-33 -0.07873493552653343 1102 34-35 -0.05567044523483844 1102 36-37 0.05207848799616244 1102 38-39 0.031981232216409694 1102 40-41 -0.00803228821079216 1102 42-43 0.06367209819538999 1102 44-45 -0.020922887867804718 1102 46-47 0.07245162326272947 1102 48-49 0.2162245205567146 1102 50-51 0.3302436058508462 1102 52-53 0.21411465401070728 1102 54-55 0.4391400356716204 1102 56-57 0.44245526286589865 1102 58-59 0.33959636101508295 1102 60-61 0.2705335975651266 1102 62-63 0.28737062566695926 1102 64-65 0.264264319009051 1102 66-67 0.34998531774350994 1102 68-69 0.1899527274709243 1102 70-71 0.1527739435942479 1102 72-73 0.35755272741862854 1102 74-75 0.3249268644376535 1102 76-77 0.29415980489296345 1102 78-79 0.2871464466747966 1102 80-81 0.18461276916959335 1102 82-83 0.403160377501397 1102 84-85 0.48684841052571315 1102 86-87 0.41734777447792837 1102 88-89 0.44199310703238837 1102 90-91 0.8488916960335722 1102 92-93 0.9344049791532925 1102 94-95 0.7917150113331743 1102 96-97 1.0787642260248944 1102 98-99 1.183447462970431 1102 100-101 2.1843693508131814 1103 1 -0.037054483140202876 1103 2 0.06477925802222018 1103 3 0.056624336164908584 1103 4 0.016125493604661756 1103 5 0.004869820818925064 1103 6 -0.08976489063282145 1103 7 -0.10627364897361957 1103 8 0.012142957291892742 1103 9 0.015971558452072543 1103 10-11 0.019327000459519184 1103 12-13 0.08043534981572975 1103 14-15 -0.08563708644176415 1103 16-17 -0.06074338514184063 1103 18-19 -0.007354016729394175 1103 20-21 0.10549028661993276 1103 22-23 0.06410569606910599 1103 24-25 -0.0574348335133692 1103 26-27 -0.08621556120127849 1103 28-29 0.0418701523622218 1103 30-31 -0.0852392795230088 1103 32-33 -0.15713759388135884 1103 34-35 -0.042166396796893935 1103 36-37 0.06487585113746519 1103 38-39 0.06954527277244438 1103 40-41 0.07079044036473192 1103 42-43 0.17361943267518143 1103 44-45 0.09623505495248708 1103 46-47 0.05091921784570985 1103 48-49 -0.03685211314921588 1103 50-51 -0.07017825571407599 1103 52-53 -0.11313617000764253 1103 54-55 0.042031265250294325 1103 56-57 0.02898166727976559 1103 58-59 0.07371788580728378 1103 60-61 0.03846987945104985 1103 62-63 0.15517479591631655 1103 64-65 0.24314078791131521 1103 66-67 0.11139841963517227 1103 68-69 0.24605091104788102 1103 70-71 0.16370159120101135 1103 72-73 0.012852419307421314 1103 74-75 0.12899545500802034 1103 76-77 0.017189326245784287 1103 78-79 -0.2911814230586529 1103 80-81 -0.024392318248008138 1103 82-83 0.08452009733516164 1103 84-85 0.07566907905538045 1103 86-87 0.19475399760776568 1103 88-89 0.5418278555276572 1103 90-91 0.5563056219435758 1103 92-93 0.14848404908737223 1103 94-95 0.2126665733853379 1103 96-97 0.2205800220982681 1103 98-99 0.41542116603720913 1103 100-101 1.2762997929169337 1104 1 0.060408616490796874 1104 2 0.07003756060524324 1104 3 0.11318568302837662 1104 4 0.05091562275595152 1104 5 0.0583561369320833 1104 6 -0.10909145889850436 1104 7 0.046182531838184104 1104 8 0.041905411166432316 1104 9 -0.04390313404485369 1104 10-11 -0.06286857149619607 1104 12-13 -0.045379533333104405 1104 14-15 -0.05178484166931696 1104 16-17 -0.0034267338503326528 1104 18-19 -0.16002160590528547 1104 20-21 -0.06278500550799748 1104 22-23 -0.1279184429222795 1104 24-25 -0.14605721973723007 1104 26-27 -0.016204798593655312 1104 28-29 -0.15943673570666306 1104 30-31 -0.11110605381575311 1104 32-33 -0.07828227407816257 1104 34-35 -0.2579143980269052 1104 36-37 -0.15658786349968068 1104 38-39 0.04163360303074626 1104 40-41 -0.11305027304240411 1104 42-43 -0.24296267064585209 1104 44-45 -0.23258759264898998 1104 46-47 -0.22307178768935643 1104 48-49 -0.19454353381342315 1104 50-51 0.16971642448272917 1104 52-53 0.12907247082876694 1104 54-55 0.31381785811621654 1104 56-57 0.37943907810387856 1104 58-59 0.27304137904221903 1104 60-61 0.1806765983932408 1104 62-63 0.17461667783513235 1104 64-65 0.05421089861241768 1104 66-67 0.3021560303362847 1104 68-69 0.46014031756694607 1104 70-71 0.39771719698206454 1104 72-73 0.3059297563307908 1104 74-75 0.2526866450449141 1104 76-77 0.25575021185463953 1104 78-79 0.41978682724885275 1104 80-81 0.40714980967327463 1104 82-83 0.1277761705208924 1104 84-85 0.1433947094366843 1104 86-87 0.12278098100259527 1104 88-89 0.4429291009151122 1104 90-91 0.19606769143927139 1104 92-93 0.2259940429373124 1104 94-95 0.41820162873588984 1104 96-97 0.43492311865423616 1104 98-99 0.9191957662832131 1104 100-101 1.702635509399098 1105 1 -0.17477442448917202 1105 2 -0.11163814178227938 1105 3 -0.1622631271781998 1105 4 -0.16239051323795906 1105 5 -0.07618773050072036 1105 6 -0.2684916746015418 1105 7 -0.16574029120236133 1105 8 -0.09986522941387221 1105 9 0.02260951055373539 1105 10-11 -0.14521356650039507 1105 12-13 -0.08272447423119189 1105 14-15 -0.08491311283472669 1105 16-17 -0.15584816275670477 1105 18-19 -0.015229138919032437 1105 20-21 -0.13596713029111385 1105 22-23 -0.07961070178035357 1105 24-25 -0.09609563507739693 1105 26-27 -0.3231595983469262 1105 28-29 -0.3010306296514642 1105 30-31 -0.4713111881251635 1105 32-33 -0.4917386447122567 1105 34-35 -0.49689208105886706 1105 36-37 -0.5375679416289074 1105 38-39 -0.45243817053156477 1105 40-41 -0.6277310650116164 1105 42-43 -0.7269120922515029 1105 44-45 -0.7691470931413491 1105 46-47 -0.8314625636792172 1105 48-49 -0.7079023330905656 1105 50-51 -0.55123580703372 1105 52-53 -0.7508958229890652 1105 54-55 -0.7574921951602676 1105 56-57 -0.564966890882495 1105 58-59 -0.5303388101848867 1105 60-61 -0.7048051083299853 1105 62-63 -0.42193297534468144 1105 64-65 -0.3845229462040365 1105 66-67 -0.24630410969034244 1105 68-69 -0.05675792326297113 1105 70-71 -0.15601828905314363 1105 72-73 -0.44732292282356667 1105 74-75 -0.23166406356480707 1105 76-77 -0.2157530002449306 1105 78-79 -0.11345506675366579 1105 80-81 -0.1819864042695123 1105 82-83 -0.10906341928263075 1105 84-85 -0.17697479924374093 1105 86-87 -0.0902212589904785 1105 88-89 0.14463668983851008 1105 90-91 0.32245145527690866 1105 92-93 0.42431188388697905 1105 94-95 0.5544065538349976 1105 96-97 0.7020026149623924 1105 98-99 0.5085357799179526 1105 100-101 1.2521994141290556 1106 1 -0.06860210218781759 1106 2 0.04739830564126635 1106 3 -0.13483399716842825 1106 4 -0.1477137921096272 1106 5 -0.02182660775250156 1106 6 0.023925585557655893 1106 7 -0.001422458497430057 1106 8 -0.03491061413667751 1106 9 -0.10893915583363878 1106 10-11 -0.2180539519214264 1106 12-13 -0.36563607875569915 1106 14-15 -0.254357324536997 1106 16-17 -0.2985558851418446 1106 18-19 -0.17253258815797068 1106 20-21 0.09261826281040442 1106 22-23 -0.05687644678803849 1106 24-25 -0.12172054779908592 1106 26-27 -0.10865603739176066 1106 28-29 0.04651300950507675 1106 30-31 0.016323220476991196 1106 32-33 0.0659278823091114 1106 34-35 -0.12541044441594096 1106 36-37 0.11297108923270827 1106 38-39 0.09479824896291689 1106 40-41 -0.014090512016224466 1106 42-43 0.14641705172280695 1106 44-45 0.14979160257153268 1106 46-47 0.40367219403618293 1106 48-49 0.4018086011365014 1106 50-51 0.7321729347621186 1106 52-53 0.6211793061828317 1106 54-55 0.5621503128693419 1106 56-57 0.5202614291845293 1106 58-59 0.3875274096168084 1106 60-61 0.48259190326057677 1106 62-63 0.44642479591631457 1106 64-65 0.4355217402922662 1106 66-67 0.45489544344469124 1106 68-69 0.3361550777145439 1106 70-71 0.6519009959629116 1106 72-73 0.6446083716883706 1106 74-75 0.6960639073889716 1106 76-77 0.8338559929124507 1106 78-79 0.529517981703254 1106 80-81 0.08388149127580391 1106 82-83 0.4247433116208761 1106 84-85 0.7722613409601422 1106 86-87 0.7515843547506194 1106 88-89 1.0883903555276575 1106 90-91 1.1476895505150004 1106 92-93 1.0485138109921301 1106 94-95 0.48504752576629073 1106 96-97 1.3333627601935056 1106 98-99 1.3086949755610213 1106 100-101 0.9476539595836009 1107 1 -0.24955448314020146 1107 2 -0.2367883987434567 1107 3 -0.15714422819152674 1107 4 -0.18213151959665907 1107 5 -0.27721557522067997 1107 6 -0.3090305672004803 1107 7 -0.3313045895676794 1107 8 -0.2585975542592607 1107 9 -0.3036963458383539 1107 10-11 -0.33471590383090444 1107 12-13 -0.13330227394665428 1107 14-15 -0.176262086441767 1107 16-17 -0.2806020897623043 1107 18-19 -0.382952201547873 1107 20-21 -0.3862124774064739 1107 22-23 -0.40081798379887346 1107 24-25 -0.5325173417641906 1107 26-27 -0.2871850331484751 1107 28-29 -0.23564634928793993 1107 30-31 -0.2543038422292838 1107 32-33 -0.7274243100529816 1107 34-35 -0.5947550931665262 1107 36-37 -0.534959132360882 1107 38-39 -0.486606748679705 1107 40-41 -0.3331287015494624 1107 42-43 -0.22966027029511338 1107 44-45 -0.4762432866316644 1107 46-47 -0.5002410544315197 1107 48-49 -0.5356454299808959 1107 50-51 -0.5052339487833848 1107 52-53 -0.40497816670730913 1107 54-55 -0.3949736852447572 1107 56-57 -0.2896961380007568 1107 58-59 -0.15383986996829435 1107 60-61 0.07825432664576937 1107 62-63 0.025038657302452805 1107 64-65 0.01706818065058968 1107 66-67 0.2782146325064545 1107 68-69 0.13780524273104788 1107 70-71 0.21222159945182995 1107 72-73 -0.025462349669474804 1107 74-75 0.1764222289354116 1107 76-77 0.05200780809396832 1107 78-79 0.13287488882253484 1107 80-81 0.16828507449126917 1107 82-83 0.34801535311073906 1107 84-85 0.3224357869761718 1107 86-87 0.5427583292909297 1107 88-89 0.6792814446365689 1107 90-91 0.4566098711184914 1107 92-93 0.0500413923216918 1107 94-95 0.1823035370817081 1107 96-97 0.4501850138474417 1107 98-99 0.8872239713177379 1107 100-101 1.4793371279004326 1108 1 4.4551685979854483E-4 1108 2 0.1040860426829866 1108 3 -0.1055714602952662 1108 4 -0.004772367752273965 1108 5 -0.1506888074996624 1108 6 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1.090690825011226 2316 90-91 1.2708082627886448 2316 92-93 1.5951536500263366 2316 94-95 1.627455305779705 2316 96-97 1.5466290244456857 2316 98-99 1.7784199923282884 2316 100-101 2.48544738681365 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 4.0 5 12.0 6 18.0 7 64.0 8 132.0 9 263.0 10 481.0 11 730.0 12 1178.0 13 1672.0 14 2067.0 15 3043.0 16 4296.0 17 6183.0 18 5701.0 19 3516.0 20 3272.0 21 3303.0 22 3423.0 23 3785.0 24 4458.0 25 5256.0 26 6351.0 27 7770.0 28 9419.0 29 12141.0 30 15127.0 31 18879.0 32 24707.0 33 32804.0 34 45237.0 35 65635.0 36 102618.0 37 150460.0 38 111631.0 39 8952.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.400259515831365 19.904292247340866 14.86330259485725 21.832145641970513 2 15.7920158172946 21.198214234662323 39.40209663416036 23.607673313882714 3 20.19910049669796 24.663212903012237 30.099354638731608 25.038331961558196 4 13.11231452822722 17.100945095389783 37.735502694146305 32.05123768223669 5 13.69099119297364 38.0859877547274 34.211528131173985 14.011492921124976 6 32.040403918812984 37.1256520947533 16.754114196894623 14.079829789539097 7 28.498816561814895 33.2671771576117 20.458960349930557 17.77504593064285 8 26.343499516242368 35.331009089828456 19.432762203406917 18.89272919052226 9 26.10094359070042 17.38337528908844 20.44511720777804 36.070563912433094 10-11 25.066996293950094 27.082001056291933 27.308080633293656 20.54292201646431 12-13 26.31464503775154 24.351790893735163 27.846300274248826 21.487263794264475 14-15 22.643769307045407 25.96755287854599 26.57461980261485 24.814058011793755 16-17 21.7669868144071 29.35460863781977 27.176044141567196 21.70236040620594 18-19 21.820102348970565 27.987899288131462 29.338207523747762 20.85379083915021 20-21 22.627970068719165 26.659258579362582 29.55187341349315 21.1608979384251 22-23 22.342414459582578 26.784896104101293 29.486287276166202 21.386402160149927 24-25 22.6627283930369 26.843507792033872 29.380790232760397 21.112973582168827 26-27 22.320354449693376 26.943954786447673 29.776497770394663 20.95919299346429 28-29 21.749257976052093 27.761664541224178 29.45163583834599 21.037441644377736 30-31 22.11095880310989 27.042126788135224 29.335574317360052 21.511340091394832 32-33 22.015185325065566 27.212833796526876 29.38131687403794 21.390664004369615 34-35 22.506541637011747 26.994352900815393 29.380489294887518 21.118616167285346 36-37 22.544384574526514 26.764662069339096 29.544650904544014 21.146302451590383 38-39 21.967787610086837 27.37676970277871 29.606719340825684 21.04872334630877 40-41 22.16121542788099 27.429208127128195 29.170660363021355 21.238916081969457 42-43 22.046691927780103 27.43225998681767 28.838525505397833 21.68252258000439 44-45 22.544986450272273 27.22080865015822 28.786889340630072 21.447315558939433 46-47 22.62044662189714 27.852176307462205 28.38468587352484 21.142691197115813 48-49 22.79995606307056 27.799888352049162 28.19584735829212 21.20430822658816 50-51 22.179873575999604 28.09856919088339 28.4143282540036 21.307228979113408 52-53 22.7318859715448 28.458211359359964 27.546067980411937 21.263834688683296 54-55 22.399106816393292 28.017767372014884 27.83607613126308 21.747049680328743 56-57 22.63722390831025 28.089465820228742 27.88934213476299 21.38396813669802 58-59 23.090812517209887 27.805455702697458 27.68681094631419 21.416920833778473 60-61 22.546942546446 27.771901129871246 28.556972805749115 21.12418351793364 62-63 22.22245628501224 27.88655845943884 28.558101322772423 21.332883932776497 64-65 22.205603764130913 28.317050086594875 28.02664503926487 21.450701110009344 66-67 22.25796695401218 27.847135598091455 27.865568042805407 22.029329405090966 68-69 22.249164521230412 27.903335745851948 28.02799925969283 21.819500473224803 70-71 22.515870711071052 28.054030385697025 27.884677597733337 21.545421305498586 72-73 22.375065454085842 27.56391930037858 28.16496837138197 21.896046874153612 74-75 22.154970967018713 28.343231681535507 27.734208661292918 21.767588690152863 76-77 22.33019204350358 27.86842695259777 27.75316774728441 22.04821325661424 78-79 22.39970869213905 27.81027070866355 27.64558245772951 22.144438141467884 80-81 22.38082484061578 27.793493422250442 27.89829503648119 21.927386700652583 82-83 22.384277754455024 27.806893604599548 27.9620275449264 21.846801096019032 84-85 21.915123482332692 28.01325330392167 27.89242674796002 22.179196465785623 86-87 21.993216860345267 27.89415714072908 27.73789515023571 22.374730848689943 88-89 22.063862026004042 27.729393655326827 27.449822371420534 22.7569219472486 90-91 22.190269165626436 27.067047996550876 27.952052356863977 22.79063048095871 92-93 22.32143327367128 27.18641119570132 28.063511123249167 22.428644407378233 94-95 21.879462344396313 27.567338610780496 27.970971532781913 22.58222751204128 96-97 21.328219395746846 27.737218040021727 28.302680303164813 22.631882261066615 98-99 21.614626828391167 28.20977539434265 27.344350702014935 22.831247075251245 100-101 21.43282813917896 28.097794271456454 27.64504580156118 22.824331787803406 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 91.0 1 76.0 2 73.5 3 113.5 4 110.5 5 103.0 6 181.5 7 389.5 8 662.5 9 811.5 10 784.0 11 761.5 12 824.0 13 892.5 14 942.0 15 997.0 16 1073.5 17 1122.5 18 1194.5 19 1260.0 20 1324.5 21 1485.0 22 1721.0 23 2024.0 24 2491.5 25 3162.5 26 4029.5 27 4981.0 28 5873.0 29 6876.5 30 8080.0 31 9279.0 32 10402.5 33 11617.0 34 13105.0 35 14390.5 36 15880.0 37 17576.0 38 18714.0 39 19148.0 40 19666.0 41 21020.0 42 22327.5 43 23460.0 44 24859.0 45 25946.5 46 26084.5 47 27953.0 48 32121.0 49 31049.0 50 26523.0 51 23810.0 52 21911.0 53 21050.0 54 20217.5 55 18605.5 56 16457.0 57 14566.0 58 12982.0 59 11367.0 60 9355.5 61 7264.0 62 5615.0 63 4335.5 64 3214.5 65 2373.5 66 1679.5 67 1260.5 68 967.5 69 611.5 70 413.0 71 278.5 72 196.5 73 152.0 74 120.0 75 85.5 76 49.5 77 27.0 78 15.0 79 9.0 80 4.5 81 1.5 82 1.5 83 1.5 84 1.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.041378957521114555 2 0.0 3 0.0 4 0.0 5 9.028136186424993E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.00699680554447937 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.275791504222911E-4 24-25 0.0 26-27 0.0014294548961839573 28-29 0.0032350821334689557 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.009479542995746243 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 7.523446822020827E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.5046893644041654E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.009378728808331E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.010908997891930202 92-93 0.0018808617055052072 94-95 0.0 96-97 0.0 98-99 3.009378728808331E-4 100-101 0.013843142152518322 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 664589.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.79943740414939 #Duplication Level Percentage of deduplicated Percentage of total 1 82.54366490331742 53.487830470115995 2 10.319144157498199 13.373494717963968 3 3.0269774815038577 5.88439313509437 4 1.3548074388202132 3.511630393060254 5 0.7246614810561537 2.3478828140448207 6 0.4701044612529259 1.8277502766222204 7 0.28213269258376983 1.2797427826922279 8 0.20958493921425042 1.0864788919573019 9 0.15803614329947546 0.9216587857794788 >10 0.8437093847703412 9.658638648151783 >50 0.04834886086831873 2.1296702016252054 >100 0.015573823946070335 1.760332623047639 >500 0.0018595610680115298 0.7957420421866683 >1k 0.0013946708010086474 1.9347542176581027 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3771 0.5674183593168108 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2982 0.4486983684653222 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2304 0.34668042955871975 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1653 0.24872515193600855 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1090 0.16401114072005404 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1045 0.1572400385802353 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 813 0.12233124532605867 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 809 0.12172936958029698 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 752 0.11315264020319325 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 704 0.10593013125405326 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.5046893644041657E-4 0.0 4 0.0 0.0 0.0 3.0093787288083313E-4 0.0 5 0.0 0.0 0.0 3.0093787288083313E-4 0.0 6 0.0 0.0 0.0 3.0093787288083313E-4 0.0 7 0.0 0.0 0.0 4.514068093212497E-4 0.0 8 0.0 0.0 0.0 4.514068093212497E-4 0.0 9 0.0 0.0 0.0 4.514068093212497E-4 0.0 10-11 0.0 0.0 0.0 4.514068093212497E-4 0.0 12-13 0.0 0.0 0.0 4.514068093212497E-4 0.0 14-15 0.0 0.0 0.0 4.514068093212497E-4 0.0 16-17 7.523446822020828E-5 0.0 0.0 4.514068093212497E-4 0.0 18-19 2.2570340466062484E-4 0.0 0.0 5.266412775414579E-4 0.0 20-21 3.0093787288083313E-4 0.0 0.0 6.018757457616663E-4 0.0 22-23 3.0093787288083313E-4 0.0 0.0 6.018757457616663E-4 0.0 24-25 3.0093787288083313E-4 0.0 0.0 8.275791504222911E-4 0.0 26-27 3.0093787288083313E-4 0.0 0.0 0.0012037514915233325 0.0 28-29 3.0093787288083313E-4 0.0 0.0 0.0021817995783860403 0.0 30-31 3.0093787288083313E-4 0.0 0.0 0.0033103166016891644 0.0 32-33 3.0093787288083313E-4 0.0 0.0 0.0067711021398187445 1.5046893644041657E-4 34-35 3.0093787288083313E-4 0.0 0.0 0.009780480868627078 1.5046893644041657E-4 36-37 4.514068093212497E-4 0.0 0.0 0.013843142152518324 1.5046893644041657E-4 38-39 9.028136186424993E-4 0.0 0.0 0.01963619620547436 1.5046893644041657E-4 40-41 9.028136186424993E-4 0.0 0.0 0.026031126004192065 1.5046893644041657E-4 42-43 9.028136186424993E-4 0.0 0.0 0.04017520602959122 1.5046893644041657E-4 44-45 9.028136186424993E-4 0.0 0.0 0.058758119679982665 1.5046893644041657E-4 46-47 9.028136186424993E-4 0.0 0.0 0.07568587502952953 1.5046893644041657E-4 48-49 9.028136186424993E-4 0.0 0.0 0.09667629166296764 1.5046893644041657E-4 50-51 9.028136186424993E-4 0.0 0.0 0.1317355538535847 1.5046893644041657E-4 52-53 9.028136186424993E-4 0.0 0.0 0.20147790589371778 1.5046893644041657E-4 54-55 9.028136186424993E-4 0.0 0.0 0.2600103221690398 1.5046893644041657E-4 56-57 0.0010532825550829158 0.0 0.0 0.3573637240459894 1.5046893644041657E-4 58-59 0.0010532825550829158 0.0 0.0 0.4727733982957888 1.5046893644041657E-4 60-61 0.0010532825550829158 0.0 0.0 0.5859260384989821 1.5046893644041657E-4 62-63 0.0010532825550829158 0.0 0.0 0.7544512473122487 1.5046893644041657E-4 64-65 0.0010532825550829158 0.0 0.0 0.8545883245133459 1.5046893644041657E-4 66-67 0.0012037514915233325 0.0 0.0 1.080743135983292 1.5046893644041657E-4 68-69 0.0012037514915233325 0.0 0.0 1.3774678786437935 1.5046893644041657E-4 70-71 0.0012037514915233325 0.0 0.0 1.594745022863755 1.5046893644041657E-4 72-73 0.0012037514915233325 0.0 0.0 1.969036502259291 1.5046893644041657E-4 74-75 0.0012037514915233325 0.0 0.0 2.360255737004374 1.5046893644041657E-4 76-77 0.0012037514915233325 0.0 0.0 2.597996656580232 1.5046893644041657E-4 78-79 0.0012037514915233325 0.0 0.0 2.882157243047959 1.5046893644041657E-4 80-81 0.0012037514915233325 0.0 0.0 3.3934506890724943 1.5046893644041657E-4 82-83 0.0015046893644041655 0.0 0.0 3.8114533945039715 1.5046893644041657E-4 84-85 0.001579923832624374 0.0 0.0 4.419347897723254 1.5046893644041657E-4 86-87 0.0016551583008445822 0.0 0.0 5.151981149251643 1.5046893644041657E-4 88-89 0.0016551583008445822 0.0 0.0 6.17690030981554 1.5046893644041657E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTCTA 65 0.005839562 29.233593 9 GTATTAC 100 0.0016512612 23.754082 1 AGTACTT 2835 0.0 21.615845 12-13 GAGTACT 2675 0.0 21.488058 12-13 GTACTAG 155 3.898059E-5 21.455301 1 GTACATG 2260 0.0 20.811098 1 AGAGTAC 3935 0.0 20.764395 10-11 ACACCGT 115 0.0037228118 20.65106 6 ACATGGG 2230 0.0 20.02285 3 TACATGG 2270 0.0 19.879282 2 TCGTTAC 60 0.004765368 19.790602 52-53 GTACTTT 2890 0.0 19.39342 14-15 TACACTA 245 1.7528328E-7 19.38817 5 GCTAGAC 150 7.715264E-4 19.000406 3 GAGTACA 1980 0.0 18.715338 1 AGTACAT 1810 0.0 18.632996 2 TACTTTT 3300 0.0 18.279318 14-15 CCTAGAC 130 0.007558922 18.26962 3 GTATAGG 160 0.0011889252 17.815561 1 GTATCAA 6700 0.0 17.372389 1 >>END_MODULE