FastQCFastQC Report
Thu 26 May 2016
SRR937354_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937354_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences564624
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22660.40132902604210946No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17470.3094094477032503No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT15910.28178044149735043No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14450.25592252543285443No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT13800.24441043951372948No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11740.20792598260081044No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC10610.1879126640029471No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT9150.16205474793845107No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8440.14948000793448382No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC6160.10909915271047636No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGCC500.001614048838.005119
AGGACCG953.4634446E-530.0307035
GTACATG19000.028.038621
TACATGG18250.027.0937862
ACATGGG18950.025.8420663
GTATAGG1201.6934039E-423.7827591
AGTTAGC1050.002193124322.6220938
GTATCAA34150.022.146171
GAGTACT17900.022.02419312-13
TATGGGA2308.9032255E-820.6733074
CATGGGG10150.020.6122064
ACTGTGC4000.020.1902168
GTACTTT19900.019.57115414-15
CAACGCA38150.019.4432075
TCAACGC38200.019.4177554
ATCAACG38550.019.3630813
AACGCAG38800.019.2246556
GGTATCA26050.019.1722811
GTATATA2502.1359301E-719.0262071
TACACGG1759.8477045E-519.0194425
Produced by FastQC (version 0.11.5)