##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937354_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 564624 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.698225721896343 33.0 31.0 34.0 30.0 34.0 2 31.814047932783588 33.0 31.0 34.0 30.0 34.0 3 31.859290430445746 33.0 31.0 34.0 30.0 34.0 4 35.45587683130721 37.0 35.0 37.0 33.0 37.0 5 35.448948326674035 37.0 35.0 37.0 33.0 37.0 6 35.48859772166964 37.0 35.0 37.0 33.0 37.0 7 35.4253644903511 37.0 35.0 37.0 33.0 37.0 8 35.42734279803905 37.0 35.0 37.0 33.0 37.0 9 37.04944175238743 39.0 37.0 39.0 33.0 39.0 10-11 36.9454070319363 39.0 37.0 39.0 33.0 39.0 12-13 36.81189428717164 39.0 37.0 39.0 33.0 39.0 14-15 38.00753244637139 40.0 38.0 41.0 33.0 41.0 16-17 37.97796321091558 40.0 38.0 41.0 33.0 41.0 18-19 37.95373646887132 40.0 38.0 41.0 33.0 41.0 20-21 37.88294688146448 40.0 38.0 41.0 33.0 41.0 22-23 37.72449364532857 40.0 38.0 41.0 32.5 41.0 24-25 37.56294277253535 40.0 37.0 41.0 32.0 41.0 26-27 37.416805697242765 40.0 37.0 41.0 32.0 41.0 28-29 37.45974134999575 40.0 37.0 41.0 32.0 41.0 30-31 37.32056731559409 40.0 37.0 41.0 32.0 41.0 32-33 37.129162061832304 39.5 36.5 41.0 31.0 41.0 34-35 37.01441136048061 39.5 36.5 41.0 31.0 41.0 36-37 37.047250913882515 40.0 37.0 41.0 31.0 41.0 38-39 36.96811772081952 40.0 36.0 41.0 31.0 41.0 40-41 36.81036229419932 40.0 36.0 41.0 30.5 41.0 42-43 36.59714606534614 39.0 36.0 41.0 30.0 41.0 44-45 36.39032612853864 39.0 36.0 41.0 30.0 41.0 46-47 36.05861777041004 39.0 35.5 41.0 29.5 41.0 48-49 35.762634071523706 39.0 35.0 41.0 28.0 41.0 50-51 34.8761937147553 38.0 34.0 40.0 26.0 40.5 52-53 34.966921349429 38.5 34.5 40.0 26.5 40.5 54-55 35.29771405395449 39.0 35.0 40.0 26.5 41.0 56-57 35.093236383858994 39.0 35.0 40.0 26.0 41.0 58-59 34.855349754881125 38.0 34.5 40.0 26.0 41.0 60-61 34.5636937147553 38.0 34.0 40.0 25.0 41.0 62-63 34.24459020516308 38.0 34.0 40.0 23.5 41.0 64-65 33.87831282410949 37.0 34.0 40.0 23.0 41.0 66-67 33.498960370087 37.0 33.0 39.5 22.0 41.0 68-69 33.032380841055286 36.0 32.5 39.0 20.5 41.0 70-71 32.56806476522429 36.0 32.0 39.0 19.5 40.0 72-73 32.15641825356343 35.0 32.0 38.5 18.0 40.0 74-75 31.694706565785374 35.0 31.0 37.5 15.5 39.5 76-77 31.260133292244042 35.0 31.0 37.0 12.0 39.0 78-79 30.77972420584318 35.0 31.0 36.5 7.5 39.0 80-81 30.277359800504406 34.0 30.0 36.0 6.5 37.5 82-83 29.794677519907054 34.0 30.0 35.5 2.0 37.0 84-85 29.360410644960186 34.0 29.0 35.0 2.0 37.0 86-87 28.9470240372354 34.0 29.0 35.0 2.0 36.0 88-89 28.553929694805745 34.0 29.0 35.0 2.0 36.0 90-91 28.150428426705204 34.0 28.5 35.0 2.0 35.5 92-93 27.710492292215704 34.0 27.0 35.0 2.0 35.0 94-95 27.187571729150726 33.0 26.0 35.0 2.0 35.0 96-97 26.588562476975827 33.0 25.0 35.0 2.0 35.0 98-99 25.8442184533424 32.5 24.0 35.0 2.0 35.0 100-101 23.96192156195982 30.5 10.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.01656405046121634 1101 2 0.0019323492806506692 1101 3 -0.037318943139244 1101 4 -0.047099154745879446 1101 5 -0.07756002364103409 1101 6 -0.0036000656963608435 1101 7 -0.03713290435145211 1101 8 0.08257478590380884 1101 9 0.06949656914221691 1101 10-11 -0.06964898354677729 1101 12-13 0.04474798600871566 1101 14-15 0.08084155696565176 1101 16-17 0.11797329451992766 1101 18-19 0.009262429054508914 1101 20-21 0.020122170281446472 1101 22-23 0.06740278867624028 1101 24-25 0.035946514899322324 1101 26-27 0.16264555100767097 1101 28-29 -0.11279489165492151 1101 30-31 -0.014718915661980247 1101 32-33 -0.11602120947345185 1101 34-35 0.00886217473550488 1101 36-37 0.04139696262876669 1101 38-39 0.06118976482517979 1101 40-41 -0.03748377592130936 1101 42-43 0.15890664790997988 1101 44-45 0.0849088464361003 1101 46-47 0.17660011974787437 1101 48-49 0.23134834336779164 1101 50-51 0.058378117118103034 1101 52-53 0.07378357762522114 1101 54-55 0.20721155931706647 1101 56-57 0.10455301264536132 1101 58-59 0.31646739565483273 1101 60-61 0.2308681883460153 1101 62-63 0.323495754816264 1101 64-65 0.20599335808459074 1101 66-67 0.05360470410412432 1101 68-69 0.11153029830332173 1101 70-71 0.29684745917860056 1101 72-73 0.141402316848513 1101 74-75 0.1614681346264959 1101 76-77 0.13060115987696008 1101 78-79 0.23191441217291242 1101 80-81 0.005637383234930127 1101 82-83 0.0873089023762681 1101 84-85 0.24805115593482796 1101 86-87 0.20089295346483738 1101 88-89 0.24714106546473502 1101 90-91 0.33740259814601714 1101 92-93 0.4007801530520858 1101 94-95 0.5141705327942319 1101 96-97 0.32723396785539194 1101 98-99 0.46915109056832804 1101 100-101 0.849672709325155 1102 1 -0.0015122258323145843 1102 2 -0.03457917846921532 1102 3 0.0216284857275042 1102 4 -0.011342744857572029 1102 5 -0.09291446964866878 1102 6 -0.17751662947941327 1102 7 0.040381810397754236 1102 8 -0.050988049664255186 1102 9 -0.00980516217364169 1102 10-11 0.030447487784584837 1102 12-13 -0.03610762282301039 1102 14-15 0.007633023846700837 1102 16-17 0.03613143859526957 1102 18-19 0.12739157430701908 1102 20-21 0.0557941677548115 1102 22-23 0.04349828380960474 1102 24-25 0.0036088078161427006 1102 26-27 -0.0625492579159257 1102 28-29 0.027558262325960925 1102 30-31 -0.08396006527867428 1102 32-33 -0.03081434163343033 1102 34-35 0.04303398558278815 1102 36-37 0.22461554484473822 1102 38-39 0.23778886327762194 1102 40-41 0.3279625475447645 1102 42-43 0.4390573843652348 1102 44-45 0.45199202424769425 1102 46-47 0.48568680038756895 1102 48-49 0.49931009937571247 1102 50-51 0.5442088903243416 1102 52-53 0.49423234158628304 1102 54-55 0.4666869964527862 1102 56-57 0.578094027317043 1102 58-59 0.49410155110000886 1102 60-61 0.6950661268455391 1102 62-63 0.5573784387865217 1102 64-65 0.46645791348382915 1102 66-67 0.6845177363473667 1102 68-69 0.5077280071092076 1102 70-71 0.5608045064667806 1102 72-73 0.48552790184952244 1102 74-75 0.5752497533924625 1102 76-77 0.6031387004322433 1102 78-79 0.49977510297655314 1102 80-81 0.48133235007290764 1102 82-83 0.7289440340194382 1102 84-85 0.7354731318744214 1102 86-87 0.7179833092028716 1102 88-89 0.5826545743222944 1102 90-91 0.6352721895784477 1102 92-93 0.8881225975667171 1102 94-95 0.6978065832119853 1102 96-97 0.7236573506685602 1102 98-99 0.9995863600276849 1102 100-101 1.2860022048604804 1103 1 -0.27165888626542767 1103 2 -0.3315655633454959 1103 3 -0.399637078637749 1103 4 -0.42750611046725595 1103 5 -0.3968532750134415 1103 6 -0.3827348738880687 1103 7 -0.4048644056170261 1103 8 -0.23822794904901912 1103 9 -0.08161614904587822 1103 10-11 -0.4495266995981879 1103 12-13 -0.5134644330439144 1103 14-15 -0.5549764634807559 1103 16-17 -0.6592868218470542 1103 18-19 -0.6729741838116112 1103 20-21 -0.9652964550102965 1103 22-23 -0.8691190219151324 1103 24-25 -0.8556977440007856 1103 26-27 -0.5942391667859539 1103 28-29 -0.6283223233632782 1103 30-31 -0.9304317456149676 1103 32-33 -0.8080487562339158 1103 34-35 -0.5105379107317773 1103 36-37 -0.5915973505101775 1103 38-39 -0.7005462070363606 1103 40-41 -0.6188298855166536 1103 42-43 -0.6407998048693457 1103 44-45 -0.5252663614794884 1103 46-47 -0.5035773892097524 1103 48-49 -0.609627955899164 1103 50-51 -0.5831524045609981 1103 52-53 -0.6121343737899281 1103 54-55 -0.6809009287668246 1103 56-57 -0.6053664508831247 1103 58-59 -0.7174670575189523 1103 60-61 -0.7405840979036142 1103 62-63 -0.7987027264959892 1103 64-65 -0.7067386362614201 1103 66-67 -0.8607166528532488 1103 68-69 -0.7115744648536975 1103 70-71 -0.4578568569188306 1103 72-73 -0.5608280503320557 1103 74-75 -0.5206133222618377 1103 76-77 -0.6113866117281823 1103 78-79 -0.5734443860257024 1103 80-81 -0.7474601480756782 1103 82-83 -0.7353961369058091 1103 84-85 -0.7503354848725081 1103 86-87 -0.6255999297408437 1103 88-89 -0.4840347670194056 1103 90-91 -0.42664197632556267 1103 92-93 -0.2128962546925628 1103 94-95 -0.5308759811270711 1103 96-97 -0.3511635207215775 1103 98-99 -0.3254490211965724 1103 100-101 0.03691441735578849 1104 1 -0.19901615774090686 1104 2 -0.2794852752404253 1104 3 -0.18099710405878255 1104 4 -0.1707006873129302 1104 5 -0.11817305046095328 1104 6 -0.19919393542816266 1104 7 -0.22920186986869595 1104 8 -0.10983900719963913 1104 9 -0.18172948449763027 1104 10-11 -0.15777158891076226 1104 12-13 -0.24255086456599884 1104 14-15 -0.13944579935618862 1104 16-17 -0.36386578593984353 1104 18-19 -0.12631228358917923 1104 20-21 -0.0802609714810103 1104 22-23 -0.019206406726063108 1104 24-25 -0.0635745578976099 1104 26-27 -0.11032379765133271 1104 28-29 -0.11745006406871283 1104 30-31 -0.3801595286888002 1104 32-33 -0.6368449642627283 1104 34-35 -0.3402757562989862 1104 36-37 -0.20314326725628717 1104 38-39 -0.1783504650598715 1104 40-41 -0.4187864579136473 1104 42-43 -0.17739603408235638 1104 44-45 -0.3545164409202215 1104 46-47 -0.10953014845136266 1104 48-49 -0.0957014650613317 1104 50-51 -0.15639199782442148 1104 52-53 -0.18102485149355374 1104 54-55 -0.10001066290516292 1104 56-57 -0.15688376896383716 1104 58-59 -0.0014253246516773288 1104 60-61 -0.005932577937507233 1104 62-63 -0.3365234022718582 1104 64-65 -0.3119568334862919 1104 66-67 -0.18501598555105403 1104 68-69 0.02681765462516239 1104 70-71 -0.11656250250722167 1104 72-73 -0.20652871763424585 1104 74-75 -0.32088818721258505 1104 76-77 -0.2798777673261057 1104 78-79 -0.2558058943404973 1104 80-81 -0.30133579684170186 1104 82-83 -0.24709722789193123 1104 84-85 0.09086724788885192 1104 86-87 -0.07475838753132891 1104 88-89 -0.20354858970767964 1104 90-91 -0.20470468154746513 1104 92-93 3.5869711338776256E-4 1104 94-95 0.044189689882355054 1104 96-97 0.24844086440711521 1104 98-99 0.274380975625796 1104 100-101 0.23064972081940738 1105 1 0.0555229553632266 1105 2 0.04107775415668513 1105 3 -0.10270113993322383 1105 4 -0.046385789455435145 1105 5 -0.10249791344052994 1105 6 -0.10495875854724801 1105 7 -0.031698132947916235 1105 8 -0.10995858866451158 1105 9 0.1499047955222963 1105 10-11 0.12129168961440229 1105 12-13 -0.003021716583930356 1105 14-15 0.06275898390240542 1105 16-17 0.032800880726824744 1105 18-19 0.08696873285327911 1105 20-21 0.06112418048710566 1105 22-23 -0.07484915709974871 1105 24-25 -0.034050392476579816 1105 26-27 0.18618093578164974 1105 28-29 0.0039176489357757305 1105 30-31 -0.0952766188731502 1105 32-33 0.06766674475571222 1105 34-35 0.20754935580864498 1105 36-37 -0.02684281882579853 1105 38-39 0.22456433314640378 1105 40-41 0.03588718433847049 1105 42-43 0.07404143477663894 1105 44-45 0.1649902855371792 1105 46-47 0.23257249230599086 1105 48-49 0.2097494567124656 1105 50-51 0.11430306944014745 1105 52-53 0.2640000613116271 1105 54-55 0.3506644741152698 1105 56-57 0.21961099934707562 1105 58-59 0.2877874307045758 1105 60-61 0.6229588022318993 1105 62-63 0.5803948837271449 1105 64-65 0.6553351445904454 1105 66-67 0.6294683193816795 1105 68-69 0.5866980731602922 1105 70-71 0.6239357535964203 1105 72-73 0.7016028219771115 1105 74-75 0.6978786289309085 1105 76-77 0.565201953650476 1105 78-79 0.8591542451712115 1105 80-81 0.8400593202484856 1105 82-83 1.1608200615948618 1105 84-85 1.0203814481878055 1105 86-87 1.0797682691702128 1105 88-89 0.7940149379455335 1105 90-91 0.9879360758018088 1105 92-93 1.2488988067297306 1105 94-95 1.4551189375156355 1105 96-97 1.137402000431031 1105 98-99 0.9015035466272927 1105 100-101 1.4668305877850258 1106 1 0.18948057428523413 1106 2 0.04084969207983846 1106 3 -0.061119653078392844 1106 4 -0.10105199450247682 1106 5 -0.1606812203955954 1106 6 -0.08815635372882014 1106 7 0.037121659543075225 1106 8 -0.025427242493755386 1106 9 0.05428358739779071 1106 10-11 -0.25341292878004396 1106 12-13 -0.3703940018208982 1106 14-15 -0.5529261915130519 1106 16-17 -0.4938535310378853 1106 18-19 -0.1026113031970155 1106 20-21 -0.05885982769016351 1106 22-23 -0.07024807339272598 1106 24-25 -0.14687929645970854 1106 26-27 -0.40620206562518035 1106 28-29 0.12762346534305635 1106 30-31 0.17310436020009234 1106 32-33 0.19153330371113242 1106 34-35 0.28034924370101777 1106 36-37 0.2888665366652745 1106 38-39 0.40001963297934395 1106 40-41 0.1718671368575997 1106 42-43 0.3951693253947681 1106 44-45 0.6500178728052717 1106 46-47 0.6694322698492954 1106 48-49 0.6424766395138377 1106 50-51 0.5336570217834193 1106 52-53 0.5589914883757245 1106 54-55 0.49989538284647494 1106 56-57 0.6314127996636145 1106 58-59 0.41893823743982495 1106 60-61 0.44253147435007634 1106 62-63 0.5193058460941486 1106 64-65 0.7620709442914873 1106 66-67 0.9402413673901222 1106 68-69 0.528459811487906 1106 70-71 0.37578635370193325 1106 72-73 0.6740595176598703 1106 74-75 0.5887564206305527 1106 76-77 0.7719196182948096 1106 78-79 0.7680503148098303 1106 80-81 0.6291870789752956 1106 82-83 0.6748431315851917 1106 84-85 0.6485633263202253 1106 86-87 0.9991345863248782 1106 88-89 1.0586205279393788 1106 90-91 1.1761449916551463 1106 92-93 1.416506572930384 1106 94-95 1.595676617986932 1106 96-97 1.3694702761718212 1106 98-99 1.5493564658218766 1106 100-101 1.9893139365056811 1107 1 -0.23293222645149925 1107 2 -0.4812487020723424 1107 3 -0.32277140281938443 1107 4 -0.015993142194425047 1107 5 -0.206622713431166 1107 6 -0.08516697304503396 1107 7 -0.24393768200176424 1107 8 -0.12846914418594224 1107 9 -0.14354074781574155 1107 10-11 -0.08578637473377171 1107 12-13 -0.1838652807434329 1107 14-15 -0.034961128775627515 1107 16-17 -0.26367661438081313 1107 18-19 -0.41819421444370874 1107 20-21 -0.45331598344012747 1107 22-23 -0.3302468503007461 1107 24-25 -0.28665892406417015 1107 26-27 -0.023055913328647648 1107 28-29 -0.10432873334463721 1107 30-31 -0.00990077678620338 1107 32-33 -0.2135061949650492 1107 34-35 -0.306215090936945 1107 36-37 -0.2798784270736405 1107 38-39 -0.11615216108074833 1107 40-41 -0.23598367469990222 1107 42-43 -0.1987713330603924 1107 44-45 -0.17620593863711775 1107 46-47 -0.2914827468423198 1107 48-49 -0.28850859704653686 1107 50-51 -0.14764118242977275 1107 52-53 0.08531674450557603 1107 54-55 0.07204521880824899 1107 56-57 0.015246041864607207 1107 58-59 0.08753966773797117 1107 60-61 0.24608851360413553 1107 62-63 0.21740897433174666 1107 64-65 -0.058807235747650566 1107 66-67 0.208154268852077 1107 68-69 0.07275372768452826 1107 70-71 0.026786051526265453 1107 72-73 -0.16688096812348618 1107 74-75 -0.048458311152572264 1107 76-77 -0.04878948726522481 1107 78-79 0.36168160294151974 1107 80-81 0.3331412633887858 1107 82-83 0.12392473340399945 1107 84-85 0.508130783440059 1107 86-87 0.29308136023992404 1107 88-89 0.1683300795682534 1107 90-91 -0.27147789311520043 1107 92-93 0.07988468102293567 1107 94-95 0.018182949286575933 1107 96-97 -0.02340845066137831 1107 98-99 0.6785329651887473 1107 100-101 0.794110255718298 1108 1 0.21313514249549925 1108 2 0.04138942925130351 1108 3 0.10637878246603805 1108 4 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-0.07781158705275715 2316 50-51 0.002663392877856552 2316 52-53 -0.1486690981140626 2316 54-55 -0.13904956827937553 2316 56-57 -0.5509243228708627 2316 58-59 -0.43695120661673315 2316 60-61 -0.19501434516467242 2316 62-63 -0.3519058618828055 2316 64-65 -0.27956481238506825 2316 66-67 -0.3097340864412601 2316 68-69 -0.1075641455726597 2316 70-71 -0.46772636666811707 2316 72-73 -0.3581014179309818 2316 74-75 -0.7639692950266301 2316 76-77 -0.8181484564128212 2316 78-79 -0.624492013694276 2316 80-81 -0.5270792851371162 2316 82-83 -0.8885050749080854 2316 84-85 -0.9956263228332034 2316 86-87 -0.4232367917515738 2316 88-89 -0.9216198063248875 2316 90-91 -1.054698087416245 2316 92-93 -0.7398061561671909 2316 94-95 -1.2012883846313258 2316 96-97 -0.5625532008747811 2316 98-99 -1.1857030995473004 2316 100-101 -1.731669764838358 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2510.0 3 538.0 4 445.0 5 516.0 6 540.0 7 635.0 8 780.0 9 954.0 10 1145.0 11 1470.0 12 1866.0 13 2311.0 14 2589.0 15 3268.0 16 4153.0 17 5334.0 18 4694.0 19 3341.0 20 3428.0 21 3615.0 22 3940.0 23 4373.0 24 4875.0 25 5604.0 26 6385.0 27 7380.0 28 8597.0 29 10117.0 30 12685.0 31 15930.0 32 20113.0 33 26002.0 34 35728.0 35 51853.0 36 77803.0 37 114391.0 38 100062.0 39 14647.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.61839034283916 18.997914915023458 15.008701185814314 23.37499355632307 2 14.67686660898701 19.810119048676537 40.59629156532754 24.91672277700891 3 19.10399187094413 23.832558503088368 30.461753822486003 26.601695803481494 4 12.137981758295641 15.98867266195997 38.17522553412236 33.69812004562203 5 12.994853154108682 36.95620117647268 35.554809378381115 14.494136291037524 6 31.45098874569532 36.22643519011609 17.67050094081727 14.652075123371322 7 27.46770603708082 32.51245079141952 21.39335271503573 18.62649045646394 8 25.537073753454614 34.335267502933206 20.531189594179384 19.596469149432796 9 25.190139207636452 16.56309959300891 21.12750553333937 37.11925566601526 10-11 24.23632124080989 26.240725569158695 28.197465159028916 21.325488031002497 12-13 25.302064529184037 23.63815802319245 28.582306926317024 22.47747052130649 14-15 22.05696769830691 24.992412234849763 27.288354599783105 25.662265467060223 16-17 21.288667296182435 28.56250310616334 27.61396957024899 22.534860027405234 18-19 21.07725593836138 27.366514439673917 29.948811972744682 21.60741764922002 20-21 21.86716074754579 26.074390762249312 29.90875298706853 22.149695503136364 22-23 21.463401215182415 26.17691401878945 29.975712324597637 22.383972441430497 24-25 21.78300488895593 26.13444185159224 29.929726804077976 22.15282645537386 26-27 21.369983833271515 26.2927615292199 30.22219934942671 22.115055288081873 28-29 21.019925113310713 26.953439436122224 30.05868993555911 21.967945515007955 30-31 21.22978960598164 26.22762388962657 29.97191669447778 22.57066980991401 32-33 21.01702286282306 26.425642573132635 30.029288554387957 22.528046009656347 34-35 21.710147911977106 26.391512281025655 29.7269877225248 22.17135208447244 36-37 21.54353590101266 26.08764326507722 30.057448009285388 22.311372824624733 38-39 21.273610897904025 26.64039560981713 29.95459502435293 22.131398467925916 40-41 21.341192358301974 26.63316850439253 29.90895515369197 22.116683983613523 42-43 21.110802495702803 26.5908951749805 29.496040898147747 22.80226143116895 44-45 21.343093779978187 26.564944567253633 29.358279326376547 22.73368232639163 46-47 21.63209630239691 26.97340395319777 29.11303319890946 22.281466545495853 48-49 21.648221831501573 26.8056160817543 28.930684756184693 22.615477330559433 50-51 21.15534142591876 27.15616867324441 29.322633557838078 22.365856342998754 52-53 21.627092640864827 27.324633676868547 28.513097428102647 22.535176254163975 54-55 21.381093418136537 26.966051796106637 28.851491141727912 22.80136364402891 56-57 21.569250261293284 27.106982075901925 28.646083794847954 22.677683867956837 58-59 21.93190337821352 26.924206463633745 28.58434443138212 22.559545726770622 60-61 21.574211590021488 26.789969675515596 29.435510343860845 22.20030839060207 62-63 21.360629205826402 26.955798006757714 29.24193972003748 22.4416330673784 64-65 21.16676544384074 27.274300072042273 29.021325222958417 22.53760926115857 66-67 21.131962644682652 27.037800699480623 28.68198828520781 23.148248370628913 68-69 21.03645715968793 26.938677378888663 29.125982757060502 22.8988827043629 70-71 21.44830682469518 26.82249475180426 28.763839177182284 22.96535924631828 72-73 21.39802462300679 26.39280758351699 29.14816747051663 23.061000322959586 74-75 21.06330740297411 27.133797636720015 28.89041901768689 22.912475942618986 76-77 21.181912808285244 26.80166010156897 28.811653721341106 23.20477336880468 78-79 21.102855768727366 26.67845071684826 28.836671112306078 23.382022402118288 80-81 21.320993296094343 26.701154881960683 28.839927060086694 23.13792476185828 82-83 21.235133108860467 26.533934885124015 29.23115825337042 22.999773752645098 84-85 21.133466822926973 26.421358098403182 28.90046745015654 23.544707628513308 86-87 20.986344343974903 26.73774255823527 29.106012321319575 23.169900776470254 88-89 21.05296779912003 26.35054623403287 28.67522387477031 23.921262092076788 90-91 20.78736426666288 25.870476812424915 29.317424797653292 24.024734123258913 92-93 21.191325586821762 25.886786895223356 29.4182308888674 23.50365662908748 94-95 20.747381635740172 25.917392326562478 29.33996371330852 23.99526232438883 96-97 20.111257996113956 26.01919954574088 29.80516542307317 24.064377035072 98-99 20.4479267416169 26.742770437544923 28.9741519295101 23.835150891328073 100-101 20.23438816880916 26.53270522272199 29.180262422484233 24.05264418598462 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 785.0 1 444.0 2 113.0 3 120.5 4 111.5 5 110.0 6 173.5 7 361.0 8 589.0 9 706.5 10 686.0 11 685.0 12 701.5 13 707.5 14 747.5 15 786.0 16 830.0 17 874.0 18 927.0 19 990.0 20 1097.5 21 1279.0 22 1492.5 23 1770.0 24 2139.0 25 2683.0 26 3486.5 27 4359.0 28 5008.0 29 5638.5 30 6666.0 31 7759.0 32 8702.0 33 9722.0 34 11147.5 35 12419.0 36 13594.5 37 14892.5 38 15596.5 39 15965.5 40 16497.5 41 17761.0 42 18892.0 43 19709.0 44 20665.5 45 21264.0 46 21728.0 47 23641.5 48 27181.5 49 26483.0 50 22641.5 51 20446.0 52 18963.0 53 18183.0 54 17337.5 55 15835.5 56 14155.0 57 12572.0 58 11245.5 59 9782.0 60 8088.0 61 6434.0 62 4910.5 63 3703.5 64 2792.0 65 2073.0 66 1445.0 67 1012.5 68 759.5 69 590.0 70 419.0 71 268.0 72 186.5 73 129.5 74 87.5 75 70.5 76 54.5 77 33.0 78 22.0 79 15.5 80 12.0 81 8.0 82 3.0 83 4.0 84 5.0 85 4.0 86 3.0 87 3.0 88 3.0 89 2.0 90 1.0 91 2.0 92 2.5 93 1.0 94 3.0 95 3.5 96 2.0 97 3.0 98 4.0 99 2.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.36431324208676924 2 0.30905522967496957 3 0.3019708691093542 4 0.30781546657598685 5 0.3108263198163734 6 0.22740797415625266 7 0.21359347105330273 8 0.22085494063305844 9 0.2162501062654085 10-11 0.23280979908753435 12-13 0.22156337668962 14-15 0.21554167020884696 16-17 0.21748986936439116 18-19 0.21447901612400466 20-21 0.20429524781093258 22-23 0.20712899203717872 24-25 0.19641389668168552 26-27 0.1985392048513701 28-29 0.2195266230270056 30-31 0.2302417183824988 32-33 0.223865793873445 34-35 0.23307546260874493 36-37 0.2050922383745643 38-39 0.20181572161296724 40-41 0.1966795602028961 42-43 0.208191646122021 44-45 0.21137960837654793 46-47 0.21819830542095273 48-49 0.22625676556434016 50-51 0.23201280852390263 52-53 0.25857916064496017 54-55 0.2267880926067613 56-57 0.19145484428575477 58-59 0.19242894386352688 60-61 0.18649579188982401 62-63 0.19836209583722975 64-65 0.18879820907364903 66-67 0.18826688203122788 68-69 0.18259939357873559 70-71 0.1942885885120009 72-73 0.1923403893564567 74-75 0.19969041344328262 76-77 0.2062434469664768 78-79 0.20376392076851144 80-81 0.2030554847119499 82-83 0.19154339879282498 84-85 0.18321927512822694 86-87 0.1856102468191221 88-89 0.19189761682110573 90-91 0.1967681147099663 92-93 0.18746989146759616 94-95 0.18711567343931537 96-97 0.18932953611607017 98-99 0.20172716710589703 100-101 0.1822451755504548 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 564624.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.43242993633358 #Duplication Level Percentage of deduplicated Percentage of total 1 85.03013163622673 59.03848657309533 2 9.118830637496838 12.662851386785826 3 2.5452733865625405 5.301735482439542 4 1.0656646578299063 2.959667467616074 5 0.5644979910999173 1.959723360812303 6 0.36320197113398306 1.5130797248099117 7 0.25524088709347637 1.2405396507003799 8 0.18549205128948432 1.0303331083923128 9 0.1386585293045695 0.8664658758913121 >10 0.6915255028792264 8.342863701504184 >50 0.027485512886409897 1.2574140377346565 >100 0.011452284161096244 1.5228195933536997 >500 7.634856107368651E-4 0.41966773430118265 >1k 0.0017814664250526853 1.8843523025632893 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2266 0.40132902604210946 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1747 0.3094094477032503 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1591 0.28178044149735043 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1445 0.25592252543285443 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1380 0.24441043951372948 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1174 0.20792598260081044 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1061 0.1879126640029471 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 915 0.16205474793845107 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 844 0.14948000793448382 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 616 0.10909915271047636 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 1.771090141403837E-4 0.0 0.0 0.0 0.0 6 1.771090141403837E-4 0.0 0.0 0.0 0.0 7 1.771090141403837E-4 0.0 0.0 0.0 0.0 8 1.771090141403837E-4 0.0 0.0 0.0 0.0 9 1.771090141403837E-4 0.0 0.0 0.0 0.0 10-11 1.771090141403837E-4 0.0 0.0 0.0 0.0 12-13 1.771090141403837E-4 0.0 0.0 0.0 0.0 14-15 1.771090141403837E-4 0.0 0.0 0.0 0.0 16-17 2.6566352121057553E-4 0.0 0.0 0.0 0.0 18-19 3.542180282807674E-4 0.0 0.0 0.0 0.0 20-21 3.542180282807674E-4 0.0 0.0 2.6566352121057553E-4 0.0 22-23 3.542180282807674E-4 0.0 0.0 4.427725353509592E-4 0.0 24-25 3.542180282807674E-4 0.0 0.0 6.198815494913429E-4 0.0 26-27 3.542180282807674E-4 0.0 0.0 8.855450707019184E-4 0.0 28-29 3.542180282807674E-4 0.0 0.0 0.0025680807050355633 0.0 30-31 3.542180282807674E-4 0.0 0.0 0.0051361614100711266 0.0 32-33 3.542180282807674E-4 0.0 0.0 0.009740995777721103 0.0 34-35 4.427725353509592E-4 0.0 0.0 0.013548839581739351 0.0 36-37 5.313270424211511E-4 0.0 0.0 0.017268128878687408 0.0 38-39 7.084360565615348E-4 0.0 0.0 0.024086825923092182 0.0 40-41 7.084360565615348E-4 0.0 0.0 0.03108263198163734 0.0 42-43 8.855450707019184E-4 0.0 0.0 0.04711099776134206 0.0 44-45 0.0010626540848423021 0.0 0.0 0.06499900818952081 0.0 46-47 0.0012397630989826858 0.0 0.0 0.08067315594094476 0.0 48-49 0.0012397630989826858 0.0 0.0 0.10166057411658024 0.0 50-51 0.0012397630989826858 0.0 0.0 0.13292031511235797 0.0 52-53 0.0012397630989826858 0.0 0.0 0.20580067443112585 0.0 54-55 0.0012397630989826858 0.0 0.0 0.2547713168409419 0.0 56-57 0.0012397630989826858 0.0 0.0 0.3617451613817337 0.0 58-59 0.0012397630989826858 0.0 0.0 0.47633469353056196 0.0 60-61 0.0012397630989826858 0.0 0.0 0.5973001501884441 0.0 62-63 0.0012397630989826858 0.0 0.0 0.7904375301085325 0.0 64-65 0.0012397630989826858 0.0 0.0 0.883065544503953 0.0 66-67 0.0012397630989826858 0.0 0.0 1.145629657967072 0.0 68-69 0.0012397630989826858 0.0 0.0 1.4383908583411262 0.0 70-71 0.0013283176060528777 8.855450707019184E-5 0.0 1.6578289268610615 0.0 72-73 0.0014168721131230695 1.771090141403837E-4 0.0 2.0586266258607497 0.0 74-75 0.0014168721131230695 1.771090141403837E-4 0.0 2.459335770353368 0.0 76-77 0.0014168721131230695 1.771090141403837E-4 0.0 2.7027721102893256 0.0 78-79 0.0014168721131230695 1.771090141403837E-4 0.0 3.0103219133441015 0.0 80-81 0.0015939811272634532 1.771090141403837E-4 0.0 3.5539580322480093 0.0 82-83 0.0015939811272634532 1.771090141403837E-4 0.0 3.9837130550596505 3.542180282807674E-4 84-85 0.0015939811272634532 1.771090141403837E-4 0.0 4.643709796253789 3.542180282807674E-4 86-87 0.0015939811272634532 1.771090141403837E-4 0.0 5.454780526509677 3.542180282807674E-4 88-89 0.0015939811272634532 1.771090141403837E-4 0.0 6.61466746011505 3.542180282807674E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTAGCC 50 0.0016140488 38.00511 9 AGGACCG 95 3.4634446E-5 30.030703 5 GTACATG 1900 0.0 28.03862 1 TACATGG 1825 0.0 27.093786 2 ACATGGG 1895 0.0 25.842066 3 GTATAGG 120 1.6934039E-4 23.782759 1 AGTTAGC 105 0.0021931243 22.622093 8 GTATCAA 3415 0.0 22.14617 1 GAGTACT 1790 0.0 22.024193 12-13 TATGGGA 230 8.9032255E-8 20.673307 4 CATGGGG 1015 0.0 20.612206 4 ACTGTGC 400 0.0 20.190216 8 GTACTTT 1990 0.0 19.571154 14-15 CAACGCA 3815 0.0 19.443207 5 TCAACGC 3820 0.0 19.417755 4 ATCAACG 3855 0.0 19.363081 3 AACGCAG 3880 0.0 19.224655 6 GGTATCA 2605 0.0 19.172281 1 GTATATA 250 2.1359301E-7 19.026207 1 TACACGG 175 9.8477045E-5 19.019442 5 >>END_MODULE