FastQCFastQC Report
Thu 26 May 2016
SRR937354_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937354_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences564624
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33390.5913669982147411No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26100.46225452690640145No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20580.3644903511009096No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14600.25857916064496017No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT10160.17994275836662985No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT9520.16860778146164526No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA8180.14487517356683385No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA7800.1381450310294993No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC7540.1335401966618493No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT6050.10715095355493212No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC5730.10148346510243986No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT23750.023.70450812-13
GTACATG19800.023.289071
ACATGGG20650.022.318573
TATATAC3007.2759576E-1222.1728483
TACATGG20900.022.0516012
GTACTTT25800.021.53907214-15
AGTACTT24500.021.52450212-13
CTATTAC1553.877575E-521.4690071
TCTAGAC1805.1486168E-621.1169993
GTGCTAC1856.496659E-620.5571941
CTACACT2151.0739259E-619.8892654
AGAGTAC36600.019.79543110-11
TCTTACC1951.0286436E-519.4926132
ACTTTTT29150.019.06374216-17
TCTATAC1250.00601772619.0052993
CCTATGC1507.6999556E-419.0052973
AAGTATC1801.2301153E-418.4757377
TACTTTT29200.018.21780214-15
TACTGGA2905.1824827E-818.0222642
TATACTG2905.1824827E-818.0222645
Produced by FastQC (version 0.11.5)