##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937354_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 564624 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24791011363315 33.0 31.0 34.0 31.0 34.0 2 32.466106647963954 34.0 31.0 34.0 31.0 34.0 3 32.553772067783164 34.0 31.0 34.0 31.0 34.0 4 36.145131981637334 37.0 35.0 37.0 35.0 37.0 5 36.00696569752614 37.0 35.0 37.0 35.0 37.0 6 36.09860898580294 37.0 35.0 37.0 35.0 37.0 7 36.05884446994814 37.0 35.0 37.0 35.0 37.0 8 36.04182252259911 37.0 35.0 37.0 35.0 37.0 9 37.75594554960469 39.0 37.0 39.0 35.0 39.0 10-11 37.68320953413245 39.0 37.0 39.0 35.0 39.0 12-13 37.59895523392558 39.0 37.0 39.0 35.0 39.0 14-15 38.83293572359659 40.0 38.0 41.0 35.0 41.0 16-17 38.81705967156904 40.0 38.0 41.0 35.0 41.0 18-19 38.81524253308396 40.0 38.0 41.0 35.0 41.0 20-21 38.72799774717334 40.0 38.0 41.0 34.5 41.0 22-23 38.59601345320071 40.0 38.0 41.0 34.0 41.0 24-25 38.44483142764034 40.0 38.0 41.0 34.0 41.0 26-27 38.21606414180056 40.0 38.0 41.0 34.0 41.0 28-29 38.22261009096319 40.0 38.0 41.0 34.0 41.0 30-31 38.38460462183683 40.0 38.0 41.0 34.0 41.0 32-33 38.36087024285188 40.0 38.0 41.0 34.0 41.0 34-35 38.25837017200827 40.0 38.0 41.0 34.0 41.0 36-37 38.11456208025164 40.0 38.0 41.0 33.5 41.0 38-39 37.975495196803536 40.0 37.5 41.0 33.0 41.0 40-41 37.857039374876024 40.0 37.0 41.0 33.0 41.0 42-43 37.66552519907053 40.0 37.0 41.0 33.0 41.0 44-45 37.39575540536711 40.0 37.0 41.0 32.5 41.0 46-47 37.07904463855593 40.0 37.0 41.0 31.5 41.0 48-49 36.76944745529768 40.0 36.0 41.0 31.0 41.0 50-51 36.557749936240754 39.5 36.0 41.0 31.0 41.0 52-53 36.35204224404205 39.0 36.0 41.0 30.0 41.0 54-55 36.148137521607296 39.0 35.5 41.0 30.0 41.0 56-57 35.88865421944516 39.0 35.0 40.0 29.0 41.0 58-59 35.63486939981297 38.5 35.0 40.0 28.5 41.0 60-61 35.280259075065885 38.0 35.0 40.0 28.0 41.0 62-63 34.94701695287483 38.0 34.0 40.0 27.5 41.0 64-65 34.608215378729916 37.0 34.0 40.0 27.0 41.0 66-67 34.208673382640484 37.0 34.0 39.5 26.0 41.0 68-69 33.75031702513531 36.0 33.0 39.0 26.0 40.5 70-71 33.34382792796622 36.0 33.0 39.0 25.5 40.0 72-73 32.90124844144067 35.0 32.5 38.0 25.0 40.0 74-75 32.461681579245656 35.0 32.0 37.0 24.0 39.0 76-77 31.133974113746493 34.0 30.5 36.0 22.0 38.5 78-79 31.750827099096036 35.0 32.0 36.0 23.0 39.0 80-81 31.574665618181303 35.0 32.0 36.0 22.5 37.5 82-83 31.23802034628354 34.5 31.5 35.5 21.0 37.0 84-85 30.920428462127006 34.0 31.0 35.0 20.0 37.0 86-87 30.6181751041401 34.0 31.0 35.0 19.0 36.0 88-89 30.285066699254727 34.0 31.0 35.0 18.0 36.0 90-91 29.876875584459746 34.0 30.5 35.0 12.5 35.5 92-93 29.494894832667406 34.0 30.0 35.0 6.0 35.0 94-95 29.101731417722235 34.0 29.5 35.0 2.0 35.0 96-97 28.707904729519115 34.0 29.0 35.0 2.0 35.0 98-99 28.133069972229308 34.0 29.0 35.0 2.0 35.0 100-101 26.582279534699197 32.5 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.005586549711352973 1101 2 1.3278836946994943E-4 1101 3 0.04204756481239258 1101 4 0.04259609283688093 1101 5 -0.1002128517500438 1101 6 -0.04300373193088092 1101 7 -0.01382152100072176 1101 8 -0.04950498966502437 1101 9 -0.16135093418130708 1101 10-11 -0.0074976825370498545 1101 12-13 0.06921701604712638 1101 14-15 0.027542602973746 1101 16-17 -0.05681848707957471 1101 18-19 0.004894719646010515 1101 20-21 -0.05595195532014685 1101 22-23 -0.027640932070617907 1101 24-25 0.01783482725850405 1101 26-27 -0.16693456118564143 1101 28-29 -0.22878919398701214 1101 30-31 0.0125654221143634 1101 32-33 0.032183501872552256 1101 34-35 0.09040824313900231 1101 36-37 0.034310536413620696 1101 38-39 -0.03449552186908278 1101 40-41 0.04246718357320134 1101 42-43 -0.12314935211585976 1101 44-45 -0.13880491417113205 1101 46-47 -0.11543449107325898 1101 48-49 -0.0031763792329186913 1101 50-51 0.1369667671940462 1101 52-53 0.24652484692150978 1101 54-55 0.014362045805242474 1101 56-57 -0.15288127047092104 1101 58-59 -0.0712930129179199 1101 60-61 0.040802687562703 1101 62-63 -0.0812177764870512 1101 64-65 -0.09285530798431552 1101 66-67 -0.10473983630947004 1101 68-69 -0.1520767536655896 1101 70-71 -0.0387668003261723 1101 72-73 -0.14161861691201239 1101 74-75 -0.16304652693542465 1101 76-77 -0.3322368947166616 1101 78-79 -0.18587516594068987 1101 80-81 -0.1251911777113186 1101 82-83 -0.25039421697213626 1101 84-85 -0.12448816437117216 1101 86-87 -0.4207193374832663 1101 88-89 -0.2781685410722261 1101 90-91 -0.30327472201858896 1101 92-93 -0.14863357243626396 1101 94-95 -0.3938753127013186 1101 96-97 -0.5599118366873448 1101 98-99 -0.7376342891418766 1101 100-101 -0.3618570206796683 1102 1 0.05574452496779969 1102 2 -0.04226923293461482 1102 3 0.039756370692551 1102 4 0.03194979234016415 1102 5 -0.05757252328812967 1102 6 0.003262884968833646 1102 7 -0.012853936516528108 1102 8 0.04626520184205418 1102 9 0.09033817546869471 1102 10-11 0.10662991228335983 1102 12-13 -0.11185134652429696 1102 14-15 -0.02655915131147424 1102 16-17 0.01693528133180422 1102 18-19 2.0437539266282556E-4 1102 20-21 -0.06098856274498843 1102 22-23 -0.06491992371551447 1102 24-25 -0.02445780244662643 1102 26-27 -0.09566378108859652 1102 28-29 -0.07520511175668787 1102 30-31 -0.07685603701452237 1102 32-33 -0.10750354053462985 1102 34-35 0.014464058695004667 1102 36-37 -0.07550857984007564 1102 38-39 -0.032246533956708845 1102 40-41 0.02000974867699057 1102 42-43 -0.004447982567491238 1102 44-45 -0.06938747468105078 1102 46-47 -0.1713189551692622 1102 48-49 0.03759912393972087 1102 50-51 0.12019281445351027 1102 52-53 0.04407573841996992 1102 54-55 0.01021176873729246 1102 56-57 0.1352338060457825 1102 58-59 0.06685335935804915 1102 60-61 0.05236058471737692 1102 62-63 -0.011942747057560155 1102 64-65 -0.08997150425753375 1102 66-67 -0.006938291619697168 1102 68-69 0.02982109008531353 1102 70-71 -0.07877828500847528 1102 72-73 -0.026604117500603763 1102 74-75 -0.163995161693812 1102 76-77 -0.2870917570440348 1102 78-79 -0.20248201655368447 1102 80-81 -0.23941122422428052 1102 82-83 -0.029829744836849414 1102 84-85 0.014278380749271236 1102 86-87 -0.03597587657425194 1102 88-89 -0.07389078403068083 1102 90-91 0.0032795861728587283 1102 92-93 -0.1813060580086301 1102 94-95 -0.13973393626214303 1102 96-97 0.08518305550019889 1102 98-99 0.08338928316855032 1102 100-101 0.002184439023448448 1103 1 -0.14135131747046614 1103 2 -0.21981987590849883 1103 3 -0.09207688869011577 1103 4 -0.05523764043947921 1103 5 -0.1909107249297577 1103 6 -0.2280870625248781 1103 7 -0.2035490118787351 1103 8 -0.1705917389500513 1103 9 -0.24908900985271742 1103 10-11 -0.1586745011820625 1103 12-13 -0.1531316904385207 1103 14-15 -0.18736453354378568 1103 16-17 -0.32328803114453564 1103 18-19 -0.23111461601028083 1103 20-21 -0.30512456634542673 1103 22-23 -0.26392869381415096 1103 24-25 -0.34975631044242306 1103 26-27 -0.3506165571752575 1103 28-29 -0.3589026024877242 1103 30-31 -0.2662235723302615 1103 32-33 -0.19423377566598532 1103 34-35 -0.33163376731429395 1103 36-37 -0.3027957388567515 1103 38-39 -0.477424090949647 1103 40-41 -0.47544248409058554 1103 42-43 -0.49957517638199533 1103 44-45 -0.474058193156047 1103 46-47 -0.41082583258076255 1103 48-49 -0.41996642558248 1103 50-51 -0.3324221417833044 1103 52-53 -0.3184105595411282 1103 54-55 -0.4727504751506757 1103 56-57 -0.5710055924853137 1103 58-59 -0.6226062504840826 1103 60-61 -0.8470067204120397 1103 62-63 -0.7381087399662363 1103 64-65 -0.6391535887771838 1103 66-67 -0.6778087689546837 1103 68-69 -0.4841440755813764 1103 70-71 -0.4958997019401892 1103 72-73 -0.42026954883403533 1103 74-75 -0.5009696721311698 1103 76-77 -0.810405265250175 1103 78-79 -0.27759141458471603 1103 80-81 -0.2814099081935524 1103 82-83 -0.33956091103216224 1103 84-85 -0.4656866785554534 1103 86-87 -0.43671914025108904 1103 88-89 -0.5519198970434331 1103 90-91 -0.6967193819574469 1103 92-93 -0.6634769372171725 1103 94-95 -0.5941971298670943 1103 96-97 -0.5535514277592988 1103 98-99 -0.6346110486172414 1103 100-101 -0.09553441458519529 1104 1 -0.19903482557342045 1104 2 -0.15193617714777474 1104 3 -0.1383738911262995 1104 4 -0.06958781520909696 1104 5 -0.18373775596460717 1104 6 -0.08415315721823191 1104 7 -0.04531577387428598 1104 8 -0.13617165633169037 1104 9 -0.11702143226559514 1104 10-11 -0.07717201203896451 1104 12-13 -0.12520827130919798 1104 14-15 -0.2096413050722319 1104 16-17 -0.3527954985738333 1104 18-19 -0.3256416788214196 1104 20-21 -0.2728101928680431 1104 22-23 -0.28725779030816767 1104 24-25 -0.3585636401744452 1104 26-27 -0.2619728753618915 1104 28-29 -0.2759156307686226 1104 30-31 -0.16647672347950504 1104 32-33 -0.1548279923803264 1104 34-35 -0.19700554996443742 1104 36-37 -0.20814157086606855 1104 38-39 -0.21135375941697276 1104 40-41 -0.1982033145110904 1104 42-43 -0.13673172759478547 1104 44-45 -0.02183173409449779 1104 46-47 -0.10631571712689691 1104 48-49 -0.3669503255930735 1104 50-51 -0.08349300292089623 1104 52-53 -0.09803454005167112 1104 54-55 -0.042955961857586544 1104 56-57 -0.05569736242494372 1104 58-59 -0.007921365408350312 1104 60-61 -0.21549999442962786 1104 62-63 -0.057386358862530074 1104 64-65 -0.2592346183291383 1104 66-67 -0.31337968305276576 1104 68-69 -0.1738008915966205 1104 70-71 -0.2276940033913064 1104 72-73 -0.08613968970895058 1104 74-75 0.06645538877339163 1104 76-77 0.0032611895745269237 1104 78-79 0.053166979653180846 1104 80-81 0.10523027822738129 1104 82-83 0.02443336923476025 1104 84-85 0.03261911532231565 1104 86-87 0.04374426404930176 1104 88-89 0.1219080872802607 1104 90-91 0.4188134005867745 1104 92-93 0.13611738541814233 1104 94-95 -0.1460592207472935 1104 96-97 -0.2619424880283425 1104 98-99 -0.16213620485069313 1104 100-101 0.03707018238546311 1105 1 -0.013220176843972808 1105 2 -0.11308714874717651 1105 3 -0.06192645714523337 1105 4 -0.025761208333165087 1105 5 -0.12117671959190801 1105 6 -0.11542371028250642 1105 7 -0.024269436056648885 1105 8 -0.15954983271434742 1105 9 -0.051226713780295086 1105 10-11 -0.13001456311021542 1105 12-13 -0.11642650748258632 1105 14-15 -0.15682366680616155 1105 16-17 -0.26053839842435167 1105 18-19 -0.29170545560268835 1105 20-21 -0.10597411912613808 1105 22-23 -0.17054129803113938 1105 24-25 -0.23048690873448408 1105 26-27 -0.12938443490349272 1105 28-29 -0.2502355111871566 1105 30-31 -0.20542540310083268 1105 32-33 -0.1313152868496772 1105 34-35 -0.44397864413485166 1105 36-37 -0.20665178511620041 1105 38-39 -0.24483158702036434 1105 40-41 -0.19980022532325137 1105 42-43 -0.5494546972011634 1105 44-45 -0.36871265088573324 1105 46-47 -0.2626485522041193 1105 48-49 -0.24745605553826522 1105 50-51 -0.2042104917101355 1105 52-53 -0.1889114707940749 1105 54-55 -0.18402721248040876 1105 56-57 -0.2839858527089447 1105 58-59 -0.38733342320605857 1105 60-61 -0.30866641196824673 1105 62-63 -0.23222442562885703 1105 64-65 -0.3127996407506686 1105 66-67 -0.3990921892809638 1105 68-69 -0.45339730415268065 1105 70-71 -0.2558504558078525 1105 72-73 -0.14893988901139465 1105 74-75 -0.19718188078315535 1105 76-77 -0.26070243772990764 1105 78-79 -0.3512326217419357 1105 80-81 -0.502786662480144 1105 82-83 -0.43181476230485316 1105 84-85 -0.49385572274445266 1105 86-87 -0.47891891482463933 1105 88-89 -0.32401617779198233 1105 90-91 -0.5461168435587176 1105 92-93 -0.542352968343689 1105 94-95 -0.8894523448130656 1105 96-97 -0.99196740083352 1105 98-99 -1.0797470668337503 1105 100-101 -0.9563727672906701 1106 1 -0.17594659027930248 1106 2 0.0029167640286971164 1106 3 -0.1034392506034223 1106 4 0.0314906161634525 1106 5 -0.06431782132408159 1106 6 0.012807627095490659 1106 7 -0.03659028367820838 1106 8 -0.047887342606195205 1106 9 -0.08854921004337513 1106 10-11 -0.08626923426118793 1106 12-13 -0.5725121928778165 1106 14-15 -0.2856952266408541 1106 16-17 -0.061643537789521474 1106 18-19 -0.042128606925992074 1106 20-21 -0.28832898371771165 1106 22-23 -0.29869569880489877 1106 24-25 -0.08611674474961006 1106 26-27 0.17806797431131827 1106 28-29 0.032013290800001926 1106 30-31 -0.19073325942721908 1106 32-33 -0.3108083845371823 1106 34-35 -0.28266731467032713 1106 36-37 -0.3059316035458082 1106 38-39 -0.32385784438429965 1106 40-41 -0.32682668052415664 1106 42-43 -0.4814784596209378 1106 44-45 -0.5373178452056138 1106 46-47 -0.689604115819705 1106 48-49 -1.0006104563120246 1106 50-51 -0.9025983950777601 1106 52-53 -0.9701587230582049 1106 54-55 -1.1964772036876496 1106 56-57 -1.1683199114445486 1106 58-59 -1.0643837837090047 1106 60-61 -0.604405223187797 1106 62-63 -0.7008095614769161 1106 64-65 -0.8336096183291417 1106 66-67 -0.5885430817455699 1106 68-69 -0.5309700092436813 1106 70-71 -0.4503819118880372 1106 72-73 -0.4877164870945663 1106 74-75 -0.6277562125337965 1106 76-77 -0.7176742679418133 1106 78-79 -0.6451867785167522 1106 80-81 -0.5705499505307898 1106 82-83 -0.7613754542946545 1106 84-85 -0.5071643814097122 1106 86-87 -0.45075736993762305 1106 88-89 -0.5723647885367313 1106 90-91 -0.44367025954394634 1106 92-93 -0.4163376799413321 1106 94-95 -0.27181902466886143 1106 96-97 -0.2066892200544821 1106 98-99 -0.65244666236703 1106 100-101 -0.7249845561766257 1107 1 -0.2501307159843762 1107 2 -0.24819840806753746 1107 3 -0.15957197766685027 1107 4 -0.04072936772051605 1107 5 -0.37227439436426124 1107 6 -0.20707553164745462 1107 7 -0.1584534124913759 1107 8 -0.1841038154967265 1107 9 -0.3260125172840773 1107 10-11 -0.1614588135175481 1107 12-13 -0.2498821134487912 1107 14-15 -0.33971632138012353 1107 16-17 -0.2366572076410236 1107 18-19 -0.19261928264834438 1107 20-21 -0.5889919715155756 1107 22-23 -0.07128692707703266 1107 24-25 0.07410650550939835 1107 26-27 -0.08182827871044651 1107 28-29 -0.2235607710165013 1107 30-31 -0.24305641471673312 1107 32-33 -0.34360163882519856 1107 34-35 -0.25233823098205477 1107 36-37 -0.32524746345141153 1107 38-39 -0.2940521721153928 1107 40-41 -0.10017874395227011 1107 42-43 -0.07478239078243831 1107 44-45 -0.12877454774965003 1107 46-47 -0.010494886511544621 1107 48-49 0.023031192128172506 1107 50-51 0.3151893193230677 1107 52-53 0.29884956518859696 1107 54-55 0.026100354732975006 1107 56-57 -0.08787283535384205 1107 58-59 -0.06909988247727483 1107 60-61 0.053222553950455165 1107 62-63 0.11269409340970071 1107 64-65 0.018916066602365333 1107 66-67 0.22305758591875957 1107 68-69 -0.03378132212500162 1107 70-71 -0.0782202047394307 1107 72-73 -0.11457630636474647 1107 74-75 -0.23629303479695096 1107 76-77 -0.32869684478076877 1107 78-79 -0.053032194080241624 1107 80-81 0.0505379538090267 1107 82-83 -0.1152339497476298 1107 84-85 -0.17040219365789966 1107 86-87 -0.0853370579772239 1107 88-89 -0.21094470680540667 1107 90-91 -0.0055299349209469995 1107 92-93 0.2924926843961835 1107 94-95 0.020079070185516912 1107 96-97 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96-97 -0.5802873610913117 2316 98-99 -0.801851008675289 2316 100-101 -0.8582347961836092 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 5.0 5 7.0 6 22.0 7 32.0 8 136.0 9 206.0 10 382.0 11 618.0 12 919.0 13 1459.0 14 1818.0 15 2490.0 16 3579.0 17 5107.0 18 4639.0 19 2804.0 20 2481.0 21 2515.0 22 2597.0 23 2794.0 24 3227.0 25 3750.0 26 4519.0 27 5542.0 28 6851.0 29 8904.0 30 11140.0 31 14109.0 32 18354.0 33 24376.0 34 34060.0 35 49843.0 36 80768.0 37 131173.0 38 119849.0 39 13544.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.44638304130351 19.862752776040384 14.849490505560938 21.841373677095163 2 15.765713111734536 21.30656861911644 39.40498455609397 23.52273371305506 3 20.25011334976905 24.76037150386806 30.139526481339797 24.849988665023094 4 13.05877893961291 17.099343988211622 37.743702003457166 32.0981750687183 5 13.699712338278847 38.23314769941476 34.18817044311241 13.878969519193982 6 31.983762645583607 37.16880614355748 16.736801836266256 14.110629374592648 7 28.472753549264645 33.4406613959024 20.39640539544901 17.690179659383944 8 26.40323471903426 35.32191334410156 19.356421264416674 18.918430672447506 9 26.046182946527246 17.47233557199127 20.540217914931 35.94126356655048 10-11 25.184459038227207 27.082182124741422 27.158604664342995 20.574754172688372 12-13 26.302547130459736 24.446465625119004 27.805266425364856 21.445720819056408 14-15 22.646132647567228 25.907772251976535 26.715920683499107 24.730174416957126 16-17 21.775110516024824 29.36635708010995 27.17330471251665 21.68522769134858 18-19 21.817351015897305 27.97401456544532 29.321637054039503 20.88699736461787 20-21 22.586889682337272 26.706091133214315 29.513800334381816 21.193218850066593 22-23 22.335042462562985 26.779664018844702 29.561205423164456 21.324088095427857 24-25 22.8085260883426 26.732226984520562 29.485140448443204 20.974106478693635 26-27 22.280403924475365 26.956193694591228 29.919121234904416 20.844281146028987 28-29 21.852083084266503 27.650624548912294 29.537599128598185 20.95969323822301 30-31 22.11848406704734 27.029343837227426 29.42309463422928 21.429077461495954 32-33 21.99879920088413 27.109988239961464 29.509726827056586 21.381485732097822 34-35 22.55793235852532 26.94864192807957 29.442602510697384 21.050823202697725 36-37 22.47319664589464 26.71701307867306 29.68864983001887 21.12114044541343 38-39 22.040822109717944 27.36767439368567 29.688348191395814 20.90315530520057 40-41 22.031426279127842 27.48417970929275 29.212323975466813 21.27207003611259 42-43 21.984769739289398 27.43705427839599 28.817042020498324 21.761133961816295 44-45 22.50258579160645 27.149660659128905 28.805010059792004 21.54274348947264 46-47 22.777281872538186 27.811694153985663 28.42413712488311 20.986886848593045 48-49 22.77280592536967 27.844154418080734 28.297642851956084 21.085396804593508 50-51 22.18327788340372 28.033725128337732 28.56151045785377 21.221486530404775 52-53 22.719447701293962 28.44371986922224 27.589520068293478 21.247312361190318 54-55 22.36948836747995 27.941869279378846 27.93540480036272 21.753237552778486 56-57 22.60442347473717 28.060089546317553 27.922652951344613 21.41283402760067 58-59 23.103428122077702 27.68745218056618 27.79566578820596 21.41345390915016 60-61 22.46495012610162 27.768656663549546 28.676074697497807 21.09031851285103 62-63 22.107101363030974 28.007930941653207 28.631797443962707 21.25317025135311 64-65 22.167406982345774 28.29263368188387 28.043264189974217 21.496695145796142 66-67 22.244626512510983 27.823471903425993 27.94479157811216 21.987110005950864 68-69 22.18432089319618 27.842953894981438 28.086655898438607 21.886069313383775 70-71 22.699708124344696 28.034674402788408 27.845521975686477 21.420095497180426 72-73 22.408275241576696 27.549395704043754 28.30644818498682 21.73588086939273 74-75 22.210533027288957 28.362326078948115 27.758561449743546 21.668579444019382 76-77 22.370816685086005 27.893872736546797 27.679393720422794 22.055916857944403 78-79 22.33982260761144 27.8092146277877 27.638835756184648 22.21212700841622 80-81 22.449364532857263 27.739965003258803 27.929205984867806 21.881464479016124 82-83 22.254495477513313 27.784911347925963 28.141963752804973 21.818629421755755 84-85 21.87553132704242 27.89431550908215 28.058672674204427 22.171480489671 86-87 21.992954603417495 27.823826121454275 27.769896426648533 22.413322848479698 88-89 22.009868514267904 27.726593272691208 27.494757573181445 22.768780639859447 90-91 22.096168254592406 26.95762992562521 28.04331769319468 22.902884126587704 92-93 22.431953270564563 27.0502575219797 28.13418772537601 22.38360148207973 94-95 21.88403255972116 27.474744254583584 28.08807277055173 22.55315041514353 96-97 21.26273413811669 27.62307305392615 28.467174615319223 22.647018192637933 98-99 21.442930138235788 28.374556113457842 27.401680791335686 22.780832956970677 100-101 21.120791584630634 28.388586429446356 27.529743321314665 22.960878664608348 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 95.0 1 80.5 2 75.0 3 90.5 4 94.0 5 101.0 6 172.0 7 347.5 8 588.0 9 739.0 10 733.5 11 707.5 12 729.5 13 771.5 14 830.5 15 873.5 16 893.5 17 947.5 18 1009.0 19 1054.0 20 1158.0 21 1321.0 22 1512.5 23 1807.0 24 2192.5 25 2707.0 26 3384.5 27 4222.0 28 5080.5 29 5950.5 30 6915.5 31 7945.0 32 8900.0 33 9854.5 34 11140.0 35 12383.5 36 13560.0 37 14769.0 38 15621.0 39 16063.5 40 16529.0 41 17670.0 42 18921.5 43 19782.0 44 21063.0 45 21921.0 46 22068.5 47 24016.5 48 27591.5 49 26383.0 50 22330.5 51 20261.0 52 18668.0 53 17907.5 54 17168.0 55 15644.5 56 13929.5 57 12369.5 58 10959.0 59 9664.5 60 7989.5 61 6179.5 62 4828.0 63 3708.0 64 2694.0 65 1967.5 66 1457.5 67 1141.5 68 818.0 69 509.5 70 339.5 71 229.0 72 171.5 73 124.5 74 89.0 75 60.0 76 36.0 77 20.0 78 12.0 79 9.0 80 5.0 81 4.0 82 3.5 83 2.0 84 1.0 85 1.5 86 1.0 87 0.5 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04268327240783247 2 0.0 3 0.0 4 0.0 5 0.012751849018107626 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.008412678171668226 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0015939811272634532 24-25 7.084360565615348E-4 26-27 0.0023909716908951796 28-29 0.002922298733316331 30-31 0.0014168721131230695 32-33 0.0 34-35 0.0 36-37 4.4277253535095917E-4 38-39 6.198815494913429E-4 40-41 1.771090141403837E-4 42-43 0.01593981127263453 44-45 0.0 46-47 0.0 48-49 1.771090141403837E-4 50-51 8.855450707019184E-5 52-53 3.542180282807674E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.771090141403837E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.009032559721159569 92-93 0.0021253081696846043 94-95 0.0 96-97 0.0 98-99 0.0015939811272634532 100-101 0.015142820709002804 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 564624.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.08444716643079 #Duplication Level Percentage of deduplicated Percentage of total 1 83.28205188703971 55.036483578410234 2 10.046292454282018 13.278073658270245 3 2.896773425766567 5.742950112245742 4 1.1972901158785867 3.1648902162267305 5 0.6783787007826212 2.2415140705350547 6 0.4179967341912087 1.6573849857839729 7 0.2742697624695148 1.268747593908632 8 0.19697792434637657 1.0413741787537059 9 0.1559304727216958 0.9274121177592135 >10 0.7954661494191295 9.266143375737181 >50 0.040118070126136406 1.8183561307319542 >100 0.015244858980574788 1.8137600307037753 >500 0.001872175664279814 0.8900892140538423 >1k 0.0013372683316284384 1.8528207368797518 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3339 0.5913669982147411 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2610 0.46225452690640145 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2058 0.3644903511009096 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1460 0.25857916064496017 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1016 0.17994275836662985 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 952 0.16860778146164526 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 818 0.14487517356683385 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 780 0.1381450310294993 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 754 0.1335401966618493 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 605 0.10715095355493212 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 573 0.10148346510243986 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 1.771090141403837E-4 0.0 0.0 0.0 0.0 6 1.771090141403837E-4 0.0 0.0 0.0 0.0 7 1.771090141403837E-4 0.0 0.0 0.0 0.0 8 1.771090141403837E-4 0.0 0.0 0.0 0.0 9 1.771090141403837E-4 0.0 0.0 0.0 0.0 10-11 1.771090141403837E-4 0.0 0.0 0.0 0.0 12-13 1.771090141403837E-4 0.0 0.0 0.0 0.0 14-15 1.771090141403837E-4 0.0 0.0 0.0 0.0 16-17 2.6566352121057553E-4 0.0 0.0 0.0 0.0 18-19 4.427725353509592E-4 0.0 0.0 0.0 0.0 20-21 5.313270424211511E-4 0.0 0.0 2.6566352121057553E-4 0.0 22-23 5.313270424211511E-4 0.0 0.0 4.427725353509592E-4 0.0 24-25 5.313270424211511E-4 0.0 0.0 6.198815494913429E-4 0.0 26-27 5.313270424211511E-4 0.0 0.0 0.0010626540848423021 0.0 28-29 5.313270424211511E-4 0.0 0.0 0.0027451897191759474 0.0 30-31 5.313270424211511E-4 0.0 0.0 0.00531327042421151 0.0 32-33 5.313270424211511E-4 0.0 0.0 0.009918104791861487 0.0 34-35 6.198815494913429E-4 0.0 0.0 0.013548839581739351 0.0 36-37 7.084360565615348E-4 0.0 0.0 0.017268128878687408 0.0 38-39 8.855450707019184E-4 0.0 0.0 0.023821162401881604 0.0 40-41 8.855450707019184E-4 0.0 0.0 0.0303741959250758 0.0 42-43 8.855450707019184E-4 0.0 0.0 0.04622545269064014 0.0 44-45 8.855450707019184E-4 0.0 0.0 0.06464479016124004 0.0 46-47 0.0010626540848423021 0.0 0.0 0.08076171044801497 0.0 48-49 0.0010626540848423021 0.0 0.0 0.10183768313072061 0.0 50-51 0.0010626540848423021 0.0 0.0 0.13292031511235797 0.0 52-53 0.0010626540848423021 0.0 0.0 0.2061548924594066 0.0 54-55 0.0010626540848423021 0.0 0.0 0.2553911983904333 0.0 56-57 0.0010626540848423021 0.0 0.0 0.36289636997364616 0.0 58-59 0.0010626540848423021 0.0 0.0 0.47748590212247444 0.0 60-61 0.0010626540848423021 0.0 0.0 0.5982742497662161 0.0 62-63 0.0010626540848423021 0.0 0.0 0.7915001841933746 0.0 64-65 0.0010626540848423021 0.0 0.0 0.885279407180708 0.0 66-67 0.0012397630989826858 0.0 0.0 1.1474007481084758 0.0 68-69 0.0012397630989826858 0.0 0.0 1.4409589390461617 0.0 70-71 0.0012397630989826858 0.0 0.0 1.6594229079883251 0.0 72-73 0.0012397630989826858 0.0 0.0 2.0623459151576977 0.0 74-75 0.0012397630989826858 0.0 0.0 2.468191221060387 0.0 76-77 0.0012397630989826858 0.0 0.0 2.7120703335316954 0.0 78-79 0.0012397630989826858 0.0 0.0 3.022896653348069 0.0 80-81 0.0012397630989826858 0.0 0.0 3.5708719430984157 0.0 82-83 0.0012397630989826858 0.0 0.0 4.002663719572672 0.0 84-85 0.0012397630989826858 0.0 0.0 4.670896029924339 0.0 86-87 0.0013283176060528777 0.0 0.0 5.49268185553572 0.0 88-89 0.0014168721131230695 0.0 0.0 6.6701025815409904 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 2375 0.0 23.704508 12-13 GTACATG 1980 0.0 23.28907 1 ACATGGG 2065 0.0 22.31857 3 TATATAC 300 7.2759576E-12 22.172848 3 TACATGG 2090 0.0 22.051601 2 GTACTTT 2580 0.0 21.539072 14-15 AGTACTT 2450 0.0 21.524502 12-13 CTATTAC 155 3.877575E-5 21.469007 1 TCTAGAC 180 5.1486168E-6 21.116999 3 GTGCTAC 185 6.496659E-6 20.557194 1 CTACACT 215 1.0739259E-6 19.889265 4 AGAGTAC 3660 0.0 19.795431 10-11 TCTTACC 195 1.0286436E-5 19.492613 2 ACTTTTT 2915 0.0 19.063742 16-17 TCTATAC 125 0.006017726 19.005299 3 CCTATGC 150 7.6999556E-4 19.005297 3 AAGTATC 180 1.2301153E-4 18.475737 7 TACTTTT 2920 0.0 18.217802 14-15 TACTGGA 290 5.1824827E-8 18.022264 2 TATACTG 290 5.1824827E-8 18.022264 5 >>END_MODULE