FastQCFastQC Report
Thu 26 May 2016
SRR937353_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937353_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740608
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28230.38117330625648116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20940.28274066712754925No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19330.26100177151745596No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18370.24803944866920152No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15430.2083423349464224No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14960.20199619771863117No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC13380.1806623746975458No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11250.15190222087798133No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT10070.1359693657103353No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC7540.10180824403733149No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCC500.001608817138.0317963
GTACATG23100.025.1228921
GGACCGT950.001225971225.0005876
TACATGG23050.024.7512192
ACATGGG23700.023.4689873
GTATAGG1852.5038935E-723.1415581
GTATCAA46500.022.8125421
CATGGGG11450.022.0038054
GAGTACT23700.021.64534612-13
TAGCCCT4500.021.1273444
AGTACTT26200.021.12096612-13
TACCTGG4750.021.0189952
AGAGTAC37500.020.70978510-11
CTATACT3302.7284841E-1120.167014
ATCAACG53050.019.9837193
CAACGCA53650.019.9333975
TCAACGC53500.019.9031414
CCCTATA1456.1058876E-419.672952
GTACCTG5850.019.5153851
GTACTTT26200.019.48996214-15
Produced by FastQC (version 0.11.5)