##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937353_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 740608 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.611894551503628 33.0 31.0 34.0 30.0 34.0 2 31.717193171016245 33.0 31.0 34.0 30.0 34.0 3 31.77745582008296 33.0 31.0 34.0 30.0 34.0 4 35.38927880876253 37.0 35.0 37.0 33.0 37.0 5 35.362830269184236 37.0 35.0 37.0 33.0 37.0 6 35.42432433892154 37.0 35.0 37.0 33.0 37.0 7 35.362576423695124 37.0 35.0 37.0 33.0 37.0 8 35.359231874351885 37.0 35.0 37.0 33.0 37.0 9 36.969159123314896 39.0 37.0 39.0 33.0 39.0 10-11 36.87245276853612 39.0 37.0 39.0 33.0 39.0 12-13 36.7628056947805 39.0 37.0 39.0 33.0 39.0 14-15 37.928090163757346 40.0 38.0 41.0 33.0 41.0 16-17 37.8758918348168 40.0 38.0 41.0 33.0 41.0 18-19 37.82664108408227 40.0 38.0 41.0 33.0 41.0 20-21 37.74672431299689 40.0 38.0 41.0 32.0 41.0 22-23 37.616959984229176 40.0 37.0 41.0 32.0 41.0 24-25 37.471498417516415 40.0 37.0 41.0 32.0 41.0 26-27 37.299836755746625 40.0 37.0 41.0 31.5 41.0 28-29 37.11870787245074 39.0 36.5 41.0 31.0 41.0 30-31 37.126899115321464 39.0 37.0 41.0 31.0 41.0 32-33 36.9806085000432 39.0 36.0 41.0 31.0 41.0 34-35 36.78210942900968 39.0 36.0 41.0 30.0 41.0 36-37 36.81483321811268 39.0 36.0 41.0 30.0 41.0 38-39 36.80977993756481 39.0 36.0 41.0 30.0 41.0 40-41 36.671294395955755 39.5 36.0 41.0 30.0 41.0 42-43 36.454762843501555 39.0 36.0 41.0 30.0 41.0 44-45 36.24607957245939 39.0 35.5 41.0 30.0 41.0 46-47 35.946809918337365 39.0 35.0 41.0 28.5 41.0 48-49 35.64929692900968 39.0 35.0 41.0 27.0 41.0 50-51 34.729106355858974 38.0 34.0 39.5 25.5 40.5 52-53 34.88041244491013 38.0 34.5 39.5 26.5 40.5 54-55 35.25824930327515 39.0 35.0 40.0 26.5 41.0 56-57 35.03279670216903 38.5 34.5 40.0 26.0 41.0 58-59 34.780450656757694 38.0 34.0 40.0 26.0 41.0 60-61 34.494689498358106 38.0 34.0 40.0 24.5 41.0 62-63 34.187966508598336 38.0 34.0 40.0 23.5 41.0 64-65 33.82815740580712 37.0 33.5 40.0 23.0 41.0 66-67 33.42866266634981 36.5 33.0 39.5 22.0 41.0 68-69 32.93228063969063 36.0 32.0 39.0 20.5 41.0 70-71 32.486361205928105 35.5 32.0 39.0 19.5 40.0 72-73 32.071053782837886 35.0 31.5 38.0 18.0 40.0 74-75 31.61232055284307 35.0 31.0 37.0 15.5 39.5 76-77 31.171388237772206 35.0 31.0 37.0 12.0 39.0 78-79 30.702893433503284 35.0 30.5 36.0 7.5 39.0 80-81 30.236600873876597 34.0 30.0 36.0 6.5 37.5 82-83 29.77313639604217 34.0 29.5 35.5 2.0 37.0 84-85 29.305320763480815 34.0 29.0 35.0 2.0 37.0 86-87 28.90816734358797 34.0 29.0 35.0 2.0 36.0 88-89 28.529712749524712 34.0 29.0 35.0 2.0 36.0 90-91 28.089972022986522 34.0 27.5 35.0 2.0 35.5 92-93 27.6353442036813 33.5 27.0 35.0 2.0 35.0 94-95 27.094084860006912 33.0 25.5 35.0 2.0 35.0 96-97 26.499128418812653 33.0 25.0 35.0 2.0 35.0 98-99 25.80546658961286 32.5 23.5 35.0 2.0 35.0 100-101 23.979755822243348 30.5 10.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.0392738284838785 1101 2 -0.026079260834858786 1101 3 -1.4893719772146596E-5 1101 4 -0.07678776088030048 1101 5 0.04382996406678075 1101 6 -0.2592535734675252 1101 7 0.004134381228197981 1101 8 -0.03360044535357787 1101 9 0.0378261674571192 1101 10-11 -0.09276132351762811 1101 12-13 0.0554047381267182 1101 14-15 -0.026051735751565275 1101 16-17 0.006720844266958181 1101 18-19 0.06808566523983473 1101 20-21 0.04663618171259287 1101 22-23 0.07978579187052048 1101 24-25 0.1518857258043269 1101 26-27 0.12245715757429565 1101 28-29 0.0070097759084433164 1101 30-31 0.07766595310131663 1101 32-33 -0.10096026896562194 1101 34-35 -0.18951503271534165 1101 36-37 -0.1810500795191956 1101 38-39 -0.11759162934823308 1101 40-41 -0.024559662789044978 1101 42-43 -0.10240408351864261 1101 44-45 -0.16672554756886626 1101 46-47 -0.0877033948289565 1101 48-49 -0.16298020152344606 1101 50-51 -0.10125282738882646 1101 52-53 -0.16251228355769598 1101 54-55 -0.0766732574607758 1101 56-57 -0.06788950231935331 1101 58-59 -0.08279028491459428 1101 60-61 0.014103641195958971 1101 62-63 0.12177184667597629 1101 64-65 0.04869647260387211 1101 66-67 -0.010222493429793644 1101 68-69 -0.029695162331933034 1101 70-71 0.020264347199038468 1101 72-73 0.089556717854677 1101 74-75 0.08267284254536733 1101 76-77 0.04060803153019066 1101 78-79 0.2210363955189969 1101 80-81 0.17402256134176142 1101 82-83 -0.08119810395204397 1101 84-85 0.1098579029563247 1101 86-87 0.15789897357166893 1101 88-89 0.11999393825350069 1101 90-91 0.07301740457744543 1101 92-93 0.08994142228634416 1101 94-95 0.1348429425043065 1101 96-97 0.23032560484256237 1101 98-99 0.35015033094052583 1101 100-101 0.5916317350542535 1102 1 0.013376923575670219 1102 2 -0.037003545752849476 1102 3 0.12926970972444352 1102 4 -0.09203962511896435 1102 5 0.14997855014215844 1102 6 0.013100100073813792 1102 7 0.03528037046338284 1102 8 0.15441224770869155 1102 9 0.37326038108270154 1102 10-11 0.012938118929810116 1102 12-13 0.3080919190994109 1102 14-15 0.24882709056444696 1102 16-17 0.284635413345093 1102 18-19 0.3981067471931823 1102 20-21 0.3687379983303245 1102 22-23 0.3652392440586709 1102 24-25 0.4714199623991462 1102 26-27 0.3681833438182096 1102 28-29 0.33858896197826027 1102 30-31 0.29858170867940004 1102 32-33 0.22430676667180194 1102 34-35 0.1997205892022791 1102 36-37 0.2562285489980596 1102 38-39 0.3750750402444538 1102 40-41 0.22704708636204174 1102 42-43 0.009909620628839377 1102 44-45 0.2881918436527897 1102 46-47 0.32682910810071775 1102 48-49 0.3648893800414754 1102 50-51 0.2151951926485438 1102 52-53 0.2616731498512763 1102 54-55 0.5008091388887195 1102 56-57 0.5643123401936307 1102 58-59 0.3659166349695937 1102 60-61 0.5797086025505962 1102 62-63 0.64881629141302 1102 64-65 0.5141438679365891 1102 66-67 0.4897548299375245 1102 68-69 0.48585808167010214 1102 70-71 0.4360089527207336 1102 72-73 0.4165010928062145 1102 74-75 0.4054227719552017 1102 76-77 0.45526196417035436 1102 78-79 0.6564270114894946 1102 80-81 0.5595392154997043 1102 82-83 0.49855268716983403 1102 84-85 0.6164765536820482 1102 86-87 0.5571503520914831 1102 88-89 0.6989920114712262 1102 90-91 0.622821141979113 1102 92-93 0.2628141712647931 1102 94-95 0.17996724702661027 1102 96-97 0.4069743789145406 1102 98-99 0.6337501512830741 1102 100-101 0.8542410400812983 1103 1 -0.04463279496183148 1103 2 -0.0951988568385218 1103 3 0.025763117607372266 1103 4 -0.029578290233160942 1103 5 -0.35716383255358863 1103 6 -0.2729481591983216 1103 7 -0.13338157889654667 1103 8 -0.01895789155734917 1103 9 0.15960233242228838 1103 10-11 -0.07047140502855598 1103 12-13 0.11459215157850622 1103 14-15 -0.04501247412009235 1103 16-17 -0.08225044788898117 1103 18-19 -0.0019887841289758512 1103 20-21 0.04931401247963407 1103 22-23 0.06594716566959136 1103 24-25 -0.08817217681219347 1103 26-27 -0.2081586856656088 1103 28-29 -0.25368309455649296 1103 30-31 -0.20438484168259663 1103 32-33 -0.30833668334389586 1103 34-35 -0.13136674869728182 1103 36-37 -0.28680580450820514 1103 38-39 -0.2298120999502018 1103 40-41 -0.1669512413514127 1103 42-43 -0.17031930289530806 1103 44-45 -0.033221238472897596 1103 46-47 -0.01823595348344753 1103 48-49 -0.09006047089840763 1103 50-51 -0.1964555912032111 1103 52-53 -0.16055217643344832 1103 54-55 -0.005452818806595872 1103 56-57 -0.025258205161939884 1103 58-59 -0.10049046261040928 1103 60-61 0.019608851439969044 1103 62-63 0.17028869803392155 1103 64-65 -0.1361779560539489 1103 66-67 -0.16406818119412492 1103 68-69 -0.2020433029154276 1103 70-71 -0.32912302637308244 1103 72-73 -0.09178610287084155 1103 74-75 -0.06807005375320685 1103 76-77 0.07189483345719339 1103 78-79 0.27873933835118336 1103 80-81 0.12154666264489933 1103 82-83 0.2039788923166732 1103 84-85 0.25098231095825696 1103 86-87 0.501723300382146 1103 88-89 0.43369755749753125 1103 90-91 0.5712602760889389 1103 92-93 0.5009541562350535 1103 94-95 0.37063884459423946 1103 96-97 0.593866400518813 1103 98-99 0.8281944871938656 1103 100-101 0.9639672617686657 1104 1 -0.29916373858130285 1104 2 -0.15768141582171324 1104 3 -0.1883958254018978 1104 4 -0.4572562072083386 1104 5 -0.2860844801129616 1104 6 -0.17859551393092943 1104 7 -0.3644383091756396 1104 8 -0.3644072279615429 1104 9 -0.18697067055803274 1104 10-11 -0.21063950852494884 1104 12-13 -0.1214141067234209 1104 14-15 -0.11187156558729328 1104 16-17 -0.1882648546339638 1104 18-19 -0.16460603427129428 1104 20-21 -0.16326919279787688 1104 22-23 -0.19663641168294532 1104 24-25 -0.2842138566565211 1104 26-27 -0.17814804431988307 1104 28-29 -0.11623671986949091 1104 30-31 -0.23042451232178962 1104 32-33 -0.28678051675846206 1104 34-35 -0.1067316612782534 1104 36-37 -0.11216029898847069 1104 38-39 0.056078256981372476 1104 40-41 -0.08383991278311953 1104 42-43 -0.16638460950373712 1104 44-45 -0.35773856414644456 1104 46-47 -0.02959168325280359 1104 48-49 -0.07295990273984643 1104 50-51 -0.2433063481898543 1104 52-53 -0.10330378286736419 1104 54-55 -0.13852638175982435 1104 56-57 0.2091565022196633 1104 58-59 -0.01679311587741239 1104 60-61 0.1736869797537608 1104 62-63 0.3221046101944509 1104 64-65 0.20239915463464797 1104 66-67 0.15625678913289676 1104 68-69 0.10864585444626584 1104 70-71 0.08005345997362667 1104 72-73 0.3059881443283672 1104 74-75 0.11990689991973014 1104 76-77 0.31133069330397234 1104 78-79 0.2957720028176425 1104 80-81 0.36038441188865633 1104 82-83 0.4656099576046806 1104 84-85 0.5982669074242857 1104 86-87 0.5383419941755605 1104 88-89 0.360416451095233 1104 90-91 0.41358418228138305 1104 92-93 0.15854281241168522 1104 94-95 0.2520687661236174 1104 96-97 0.5498889929143473 1104 98-99 0.7007383269337097 1104 100-101 1.0105640775505975 1105 1 -0.10228446626500087 1105 2 -0.055032807571471665 1105 3 0.00933836588850312 1105 4 -0.09312816987627315 1105 5 0.03203287528906884 1105 6 -0.11121732621332825 1105 7 -0.3927946408623342 1105 8 -0.2082122553955088 1105 9 -0.00913658681607643 1105 10-11 -0.07614835848576007 1105 12-13 0.011273328337367161 1105 14-15 -0.20706834277973485 1105 16-17 -0.040709303129190744 1105 18-19 0.022396098469002368 1105 20-21 -0.058572607005210386 1105 22-23 -0.099735265107185 1105 24-25 -0.18079509218760847 1105 26-27 -0.008055625573639702 1105 28-29 0.016390560977768587 1105 30-31 -0.10936415759421436 1105 32-33 -0.2634716704433657 1105 34-35 -0.3454910261955817 1105 36-37 -0.45617677759727115 1105 38-39 -0.006359082379539416 1105 40-41 -0.08133713292931333 1105 42-43 -0.21364850421562664 1105 44-45 -0.05823529212180034 1105 46-47 -0.28463187720456773 1105 48-49 -0.6130608889043785 1105 50-51 -0.6732130180161349 1105 52-53 -0.5911927345434833 1105 54-55 -0.35924586261074154 1105 56-57 -0.36327008229701363 1105 58-59 -0.18353239293313806 1105 60-61 -0.5514182901199121 1105 62-63 -0.28918706482696876 1105 64-65 -0.3771547927577572 1105 66-67 -0.19075281731271332 1105 68-69 -0.2024735607818684 1105 70-71 -0.4408610663575132 1105 72-73 -0.2086821923529456 1105 74-75 -0.35259806736002375 1105 76-77 -0.4095428187906691 1105 78-79 -0.30292101017931117 1105 80-81 -0.4637164649045751 1105 82-83 -0.1703886934826535 1105 84-85 -0.36133589248092335 1105 86-87 -0.26263232179811524 1105 88-89 -0.2685149913298943 1105 90-91 -0.05707332150103994 1105 92-93 -0.1384421854920319 1105 94-95 -0.016295586124876138 1105 96-97 -0.16566269413791446 1105 98-99 -0.4781274927130177 1105 100-101 -0.02802576185587924 1106 1 -0.02419611000119204 1106 2 0.10957952488550049 1106 3 -0.1519545581909192 1106 4 -0.08758291914642058 1106 5 0.03271675728716872 1106 6 -0.005873691638576872 1106 7 0.0302057301113976 1106 8 -0.004147673708708055 1106 9 0.14277231494040876 1106 10-11 0.17135885937259587 1106 12-13 0.21838795921851073 1106 14-15 0.23581621987450774 1106 16-17 0.17295543339881903 1106 18-19 -0.06613758755818822 1106 20-21 0.013875335703545488 1106 22-23 0.11545530248261571 1106 24-25 0.016091954377657203 1106 26-27 0.3924008334015028 1106 28-29 0.3381247824974736 1106 30-31 0.6827211986212092 1106 32-33 0.49283568935276634 1106 34-35 0.4220632891965934 1106 36-37 0.3300520325448275 1106 38-39 0.2525644486451526 1106 40-41 0.3537134324023796 1106 42-43 0.413000312700774 1106 44-45 0.5075286718130982 1106 46-47 0.8181117154340285 1106 48-49 0.6852890844822994 1106 50-51 0.7100673574737897 1106 52-53 0.7914360175331652 1106 54-55 0.9040841910998054 1106 56-57 1.0380428421049928 1106 58-59 0.6102287967634723 1106 60-61 0.7244539013670419 1106 62-63 0.8047354177937152 1106 64-65 0.9063901031276558 1106 66-67 0.9401218742145616 1106 68-69 0.8396029243227332 1106 70-71 0.7480548838997478 1106 72-73 0.8422024993356274 1106 74-75 0.920291817960706 1106 76-77 0.5874474552460072 1106 78-79 0.61686317988827 1106 80-81 0.7178929158918876 1106 82-83 0.6495035628440604 1106 84-85 0.7264171357937244 1106 86-87 0.7093494843600467 1106 88-89 1.3576612373189576 1106 90-91 1.23348459072335 1106 92-93 1.121768463066818 1106 94-95 1.343689252340262 1106 96-97 1.512536954243508 1106 98-99 1.3597392470731045 1106 100-101 1.1315332632896897 1107 1 -0.33662041466650194 1107 2 -0.41021281315304137 1107 3 -0.5864475499538493 1107 4 -0.23763497628887364 1107 5 -0.21831907360856917 1107 6 -0.36695548863696814 1107 7 -0.3395805877133924 1107 8 -0.09178073813916399 1107 9 -0.3075892520149921 1107 10-11 -0.24548074088521332 1107 12-13 -0.014218934988626586 1107 14-15 -0.030236503032767814 1107 16-17 0.12294693427352144 1107 18-19 -0.1144763626925851 1107 20-21 -0.13332066120843677 1107 22-23 0.04235751296345569 1107 24-25 -0.05265003342609731 1107 26-27 -0.09872820456833864 1107 28-29 -0.10253715771118266 1107 30-31 -0.07327776731855096 1107 32-33 -0.16557561165083712 1107 34-35 -0.06216906272384648 1107 36-37 -0.05371003444244593 1107 38-39 -0.22637365647485552 1107 40-41 -7.160283989406935E-4 1107 42-43 0.09371296435703869 1107 44-45 0.13271016355124488 1107 46-47 -0.001095953610033007 1107 48-49 0.2063102543868922 1107 50-51 -0.13026594988999562 1107 52-53 0.19095864999557222 1107 54-55 0.28948588100922024 1107 56-57 -0.11827023734057462 1107 58-59 -0.11282012278522302 1107 60-61 -0.2689893775954886 1107 62-63 -0.06646387961192346 1107 64-65 -0.1254338091613718 1107 66-67 -0.3022182891102929 1107 68-69 -0.1415042283994694 1107 70-71 -0.05666000585951281 1107 72-73 -0.058086825054736835 1107 74-75 -0.05829746889185472 1107 76-77 0.0408633953555082 1107 78-79 -0.1574761901848909 1107 80-81 0.11977759692679868 1107 82-83 0.06271155768792269 1107 84-85 0.001213333851673326 1107 86-87 -0.1354299279254363 1107 88-89 -0.2582650610995003 1107 90-91 -0.11943804598862329 1107 92-93 -0.02501851344018391 1107 94-95 0.11372239892892466 1107 96-97 0.06587781874620546 1107 98-99 0.3154676575241986 1107 100-101 0.5161151417380303 1108 1 -0.09267446841970894 1108 2 0.020425067886403525 1108 3 0.00416706659955679 1108 4 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-1.4678269007515254 2316 94-95 -1.614842129205627 2316 96-97 -1.6026879763538062 2316 98-99 -2.0646694914867787 2316 100-101 -2.3677397590427347 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3234.0 3 649.0 4 561.0 5 610.0 6 734.0 7 839.0 8 997.0 9 1302.0 10 1595.0 11 1964.0 12 2566.0 13 3110.0 14 3646.0 15 4228.0 16 5375.0 17 6990.0 18 6124.0 19 4451.0 20 4600.0 21 4819.0 22 5234.0 23 5865.0 24 6434.0 25 7558.0 26 8574.0 27 10005.0 28 11822.0 29 14349.0 30 17561.0 31 21539.0 32 27510.0 33 35626.0 34 48929.0 35 68934.0 36 102240.0 37 146124.0 38 125515.0 39 18384.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.697707322824726 19.05482123569025 14.89474447430513 23.352726967179898 2 14.75949476832711 19.846897030057058 40.698405301573025 24.695202900042805 3 19.201180185562233 23.8101624380748 30.355098761970172 26.63355861439279 4 12.081827947812398 16.02813936381629 38.112636026567934 33.77739666180337 5 13.161591110111786 37.01266597767505 35.284994656477174 14.540748255735991 6 31.575378985744564 36.2397252302604 17.661101081811122 14.52379470218392 7 27.701294412533766 32.46154928891223 21.26076646978892 18.576389828765084 8 25.64537390678248 34.43225773271221 20.381282870963112 19.541085489542194 9 25.2553699413204 16.62692440941472 20.99446766126064 37.12323798800424 10-11 24.341916041636072 26.32444112688905 28.11483824774424 21.218804583730638 12-13 25.428022107792746 23.681893576978336 28.408933259072228 22.48115105615669 14-15 22.114899942422152 25.05191140183451 27.13601294723474 25.697175708508595 16-17 21.429794329355893 28.58167580329839 27.496242601087733 22.492287266257982 18-19 21.19675202490989 27.346821681404254 29.823399304585287 21.633026989100568 20-21 21.957911460067177 26.17129881483045 29.776471447942125 22.094318277160248 22-23 21.564232126618453 26.159036288052658 29.84616550469839 22.43056608063049 24-25 21.760956250769567 26.2057660276056 29.723713996154537 22.309563725470298 26-27 21.550800727817503 26.2741035414452 29.989112510598215 22.18598322013908 28-29 21.17149670193445 26.906165501449415 29.925918708165185 21.996419088450953 30-31 21.423302637063983 26.29444056455149 29.81375160383947 22.468505194545052 32-33 21.20714822752678 26.39615722202559 29.870655439164473 22.526039111283154 34-35 21.90457760106963 26.40517710360432 29.495118723154327 22.195126572171713 36-37 21.569706036180683 26.1811632298604 29.894294272350404 22.354836461608514 38-39 21.433142953019228 26.561368898053843 29.857370370220025 22.1481177787069 40-41 21.511635380324073 26.617915583365637 29.68126425373836 22.189184782571928 42-43 21.235384098088154 26.606269268837877 29.33599170285617 22.8223549302178 44-45 21.43625159270364 26.591028891627133 29.240448683985264 22.73227083168396 46-47 21.748570361489477 26.984646778475717 28.962595297957506 22.3041875620773 48-49 21.704143837632532 26.893701791745073 28.78917983290399 22.612974537718404 50-51 21.313072936616106 27.028396948217964 29.207061813880276 22.45146830128565 52-53 21.639443898853536 27.4123672335924 28.31761196777532 22.63057689977875 54-55 21.463129734364628 26.963570644012325 28.762468471407193 22.81083115021585 56-57 21.618043505467778 27.189462895877092 28.55693222523263 22.635561373422494 58-59 21.99901922654176 26.99359115274701 28.47482963288635 22.532559987824882 60-61 21.71239238272279 26.858539469420695 29.205673384650037 22.223394763206475 62-63 21.493613805895688 27.06047013157975 29.0513148166062 22.394601245918363 64-65 21.325083271775807 27.31936586276595 28.79911411277339 22.556436752684856 66-67 21.35508916164108 27.153403486362915 28.4255078472257 23.065999504770307 68-69 21.17893768433585 27.042847092567037 28.899193657493843 22.87902156560327 70-71 21.565624346760167 26.996250191955262 28.552119490545653 22.886005970738914 72-73 21.51421944605325 26.5682257357359 28.952026284594606 22.965528533616244 74-75 21.28096000627809 27.218759576959688 28.667252531361687 22.833027885400536 76-77 21.36307663161883 26.898334212912218 28.65444728616084 23.08414186930811 78-79 21.40381335262044 26.86619977984015 28.55549022778493 23.174496639754484 80-81 21.45546153226914 26.86399154669847 28.58441699887501 23.096129922157377 82-83 21.385631634463994 26.746586395107386 28.98903437996242 22.878747590466197 84-85 21.267799040796586 26.705562432777967 28.65109483126002 23.375543695165426 86-87 21.24936494696673 26.917521916517334 28.772790032356415 23.060323104159522 88-89 21.25757314362841 26.561057043404134 28.420354715730152 23.761015097237305 90-91 21.104165398172015 26.17401717034361 29.093584461447907 23.628232970036464 92-93 21.418959466709605 26.206692651019676 29.00899755018203 23.365350332088692 94-95 20.957244417743066 26.18938403708605 29.052430321580214 23.80094122359067 96-97 20.43074837405579 26.3202044624 29.459998457602328 23.789048705941884 98-99 20.73620229596393 26.707021038217356 28.782698001179913 23.7740786646388 100-101 20.586253733927876 26.464822611368454 29.008425689423785 23.94049796527988 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1021.0 1 594.5 2 169.0 3 171.0 4 144.5 5 127.5 6 212.5 7 447.5 8 770.0 9 937.5 10 890.5 11 802.5 12 839.0 13 894.0 14 932.5 15 1023.0 16 1051.0 17 1094.0 18 1205.5 19 1277.5 20 1350.0 21 1552.5 22 1828.0 23 2223.0 24 2707.5 25 3408.5 26 4422.0 27 5597.0 28 6508.0 29 7417.0 30 8720.0 31 10097.0 32 11392.0 33 12745.0 34 14474.5 35 15956.0 36 17684.5 37 19476.0 38 20438.0 39 20967.0 40 21736.5 41 23266.0 42 24575.5 43 25930.0 44 27444.0 45 28329.5 46 28704.5 47 30639.5 48 35173.5 49 34565.0 50 29537.0 51 26945.5 52 25146.5 53 23884.0 54 22927.0 55 20964.0 56 18624.5 57 16620.0 58 14900.5 59 12919.5 60 10695.0 61 8570.0 62 6620.5 63 5034.5 64 3785.5 65 2841.5 66 2066.5 67 1487.5 68 1045.5 69 743.5 70 538.5 71 377.0 72 265.5 73 185.5 74 133.5 75 100.0 76 73.0 77 50.0 78 28.0 79 19.0 80 14.0 81 7.5 82 3.5 83 3.0 84 5.5 85 4.5 86 3.0 87 2.5 88 3.5 89 4.0 90 1.0 91 0.5 92 1.0 93 1.0 94 0.5 95 0.0 96 1.0 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3695612253715866 2 0.325678361562392 3 0.32662353093674384 4 0.32743367611475976 5 0.31474140165917736 6 0.22441021431040442 7 0.23494210162461113 8 0.23413195644659524 9 0.2268406498444521 10-11 0.2400055089872105 12-13 0.23946541220186657 14-15 0.21718641980642933 16-17 0.23392942015209123 18-19 0.21934680694780506 20-21 0.22170973038368474 22-23 0.22035948842032493 24-25 0.20996262530245421 26-27 0.227718307120636 28-29 0.22819089180781196 30-31 0.23710248876598686 32-33 0.23865526702385068 34-35 0.22441021431040442 36-37 0.21907675855513306 38-39 0.2107727704804701 40-41 0.20894994382993434 42-43 0.21043520998963017 44-45 0.22839342810231592 46-47 0.21738895610093328 48-49 0.22839342810231592 50-51 0.23777760974766676 52-53 0.25080744469408917 54-55 0.24230092032492223 56-57 0.20327892758382304 58-59 0.18842626598686485 60-61 0.19882312910473554 62-63 0.19909317749740754 64-65 0.19740537504320776 66-67 0.21023267369512616 68-69 0.19821552022122363 70-71 0.20300887919115104 72-73 0.21873919806429312 74-75 0.2065195082958866 76-77 0.21846914967162115 78-79 0.21597120203940548 80-81 0.2009835162461113 82-83 0.2182666133771172 84-85 0.1961901572761839 86-87 0.20206370981679916 88-89 0.21138037936398202 90-91 0.2083423349464224 92-93 0.21246057293466988 94-95 0.21198798824749393 96-97 0.20240127030763913 98-99 0.21225803664016593 100-101 0.19443484272381611 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 740608.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.94562523465429 #Duplication Level Percentage of deduplicated Percentage of total 1 83.57988185908228 55.95307448094804 2 9.956177221781967 13.330450181184341 3 2.773468235586709 5.57014695299417 4 1.2036175074235587 3.2230770631138514 5 0.6391142346540247 2.1392951017640627 6 0.3913934173597465 1.5721246222725735 7 0.2691531859489336 1.261303982207537 8 0.19658358256253156 1.0528328676413568 9 0.13553422489923037 0.8166081083915904 >10 0.801499348090024 9.420963844299507 >50 0.038471154206661744 1.709379101000562 >100 0.012487650150303547 1.571128516870206 >500 8.056548473943546E-4 0.33062196366554925 >1k 0.001812723406637298 2.048993213646691 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2823 0.38117330625648116 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2094 0.28274066712754925 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1933 0.26100177151745596 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1837 0.24803944866920152 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1543 0.2083423349464224 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1496 0.20199619771863117 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1338 0.1806623746975458 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1125 0.15190222087798133 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1007 0.1359693657103353 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 754 0.10180824403733149 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.3502419633598341E-4 0.0 10-11 0.0 0.0 0.0 2.7004839267196683E-4 0.0 12-13 0.0 0.0 0.0 2.7004839267196683E-4 0.0 14-15 1.3502419633598341E-4 0.0 0.0 2.7004839267196683E-4 0.0 16-17 1.3502419633598341E-4 0.0 0.0 2.7004839267196683E-4 0.0 18-19 1.3502419633598341E-4 0.0 0.0 2.7004839267196683E-4 0.0 20-21 1.3502419633598341E-4 0.0 0.0 2.7004839267196683E-4 0.0 22-23 1.3502419633598341E-4 0.0 0.0 3.375604908399585E-4 0.0 24-25 1.3502419633598341E-4 0.0 0.0 4.0507258900795024E-4 0.0 26-27 1.3502419633598341E-4 0.0 0.0 9.451693743518838E-4 0.0 28-29 4.0507258900795024E-4 0.0 0.0 0.0022954113377117178 0.0 30-31 4.0507258900795024E-4 0.0 0.0 0.004388286380919461 0.0 32-33 4.0507258900795024E-4 0.0 0.0 0.007696379191151055 0.0 34-35 5.400967853439337E-4 0.0 0.0 0.011342032492222605 0.0 36-37 6.75120981679917E-4 0.0 0.0 0.016270415658486 0.0 38-39 6.75120981679917E-4 0.0 0.0 0.02153635931558935 0.0 40-41 6.75120981679917E-4 0.0 0.0 0.026464742481852748 0.0 42-43 8.101451780159005E-4 0.0 0.0 0.03841438385758728 0.0 44-45 8.101451780159005E-4 0.0 0.0 0.05427972692706533 0.0 46-47 0.0010126814725198755 0.0 0.0 0.0723729692360871 0.0 48-49 0.0010801935706878673 0.0 0.0 0.09505703422053233 0.0 50-51 0.0010801935706878673 0.0 0.0 0.12415474853093675 0.0 52-53 0.0010801935706878673 0.0 0.0 0.18451056429312135 6.751209816799171E-5 54-55 0.0010801935706878673 0.0 0.0 0.2302162547528517 1.3502419633598341E-4 56-57 0.0010801935706878673 0.0 0.0 0.3205474421016246 1.3502419633598341E-4 58-59 0.0010801935706878673 0.0 0.0 0.4199252506049084 2.0253629450397512E-4 60-61 0.0010801935706878673 0.0 0.0 0.5192355470100242 2.7004839267196683E-4 62-63 0.0010801935706878673 0.0 0.0 0.6706651832008296 2.7004839267196683E-4 64-65 0.0010801935706878673 0.0 0.0 0.7454685879709644 2.7004839267196683E-4 66-67 0.0010801935706878673 0.0 0.0 0.952325656757691 2.7004839267196683E-4 68-69 0.0010801935706878673 0.0 0.0 1.1990148634635327 2.7004839267196683E-4 70-71 0.0011477056688558589 0.0 0.0 1.3798122623574145 2.7004839267196683E-4 72-73 0.0012152177670238507 0.0 0.0 1.7142671966816454 4.0507258900795024E-4 74-75 0.0012152177670238507 0.0 0.0 2.056080949706187 4.0507258900795024E-4 76-77 0.0012152177670238507 0.0 0.0 2.2571319780504666 4.0507258900795024E-4 78-79 0.0012152177670238507 0.0 0.0 2.5076018622537157 4.0507258900795024E-4 80-81 0.0017553145523677843 0.0 0.0 2.961890770826132 4.72584687175942E-4 82-83 0.0017553145523677843 0.0 0.0 3.335840282578638 5.400967853439337E-4 84-85 0.0017553145523677843 0.0 0.0 3.884646128586243 5.400967853439337E-4 86-87 0.0017553145523677843 0.0 0.0 4.5389733840304185 5.400967853439337E-4 88-89 0.0017553145523677843 0.0 0.0 5.464159177324577 5.400967853439337E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTACGCC 50 0.0016088171 38.031796 3 GTACATG 2310 0.0 25.122892 1 GGACCGT 95 0.0012259712 25.000587 6 TACATGG 2305 0.0 24.751219 2 ACATGGG 2370 0.0 23.468987 3 GTATAGG 185 2.5038935E-7 23.141558 1 GTATCAA 4650 0.0 22.812542 1 CATGGGG 1145 0.0 22.003805 4 GAGTACT 2370 0.0 21.645346 12-13 TAGCCCT 450 0.0 21.127344 4 AGTACTT 2620 0.0 21.120966 12-13 TACCTGG 475 0.0 21.018995 2 AGAGTAC 3750 0.0 20.709785 10-11 CTATACT 330 2.7284841E-11 20.16701 4 ATCAACG 5305 0.0 19.983719 3 CAACGCA 5365 0.0 19.933397 5 TCAACGC 5350 0.0 19.903141 4 CCCTATA 145 6.1058876E-4 19.67295 2 GTACCTG 585 0.0 19.515385 1 GTACTTT 2620 0.0 19.489962 14-15 >>END_MODULE