FastQCFastQC Report
Thu 26 May 2016
SRR937353_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937353_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740608
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39830.5378013740062219No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34090.46029748530936737No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26340.3556537331489803No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19740.26653776356723125No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT11640.1571681645350847No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT11610.15676309194607674No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC9590.12948820428620808No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA9220.1244923090217767No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA8400.11342032492222605No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT7960.10747926028344279No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG559.483663E-525.90699266-67
TATTCCG950.001225274325.0030385
ACGGATC950.001225756825.001358
GTACATG24800.024.1474111
GAGTACT29250.023.5482812-13
ACATGGG25100.023.4689873
GTGTAGG2653.092282E-1123.315741
TACATGG25850.023.1556192
GTACTTT30650.022.31466914-15
AGTACTT30600.021.96605712-13
CATGGGG13000.021.5603144
AGAGTAC44300.021.4994710-11
TAGACCC1604.981544E-520.7837774
GTAAGGT2108.589159E-720.3596174
TAAGGTG2651.8493665E-819.7193785
TAGCGAC857.960787E-519.5572468-69
AGGCGTG2502.168199E-719.0010287
GCGACGA757.7267055E-418.9984670-71
ACTTTTT37150.018.60338416-17
TACTTTT36000.018.6026614-15
Produced by FastQC (version 0.11.5)