##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937353_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 740608 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.190282848686486 33.0 31.0 34.0 31.0 34.0 2 32.410862426546835 34.0 31.0 34.0 31.0 34.0 3 32.49905753110958 34.0 31.0 34.0 31.0 34.0 4 36.09753607846526 37.0 35.0 37.0 35.0 37.0 5 35.982668294158316 37.0 35.0 37.0 35.0 37.0 6 36.06725960292084 37.0 35.0 37.0 35.0 37.0 7 36.01601521992741 37.0 35.0 37.0 35.0 37.0 8 35.99949635974767 37.0 35.0 37.0 35.0 37.0 9 37.70626566280678 39.0 37.0 39.0 35.0 39.0 10-11 37.6337077104217 39.0 37.0 39.0 35.0 39.0 12-13 37.56191129450397 39.0 37.0 39.0 35.0 39.0 14-15 38.77418823453163 40.0 38.0 41.0 35.0 41.0 16-17 38.75354573539578 40.0 38.0 41.0 35.0 41.0 18-19 38.75876442058417 40.0 38.0 41.0 35.0 41.0 20-21 38.63983780893536 40.0 38.0 41.0 34.0 41.0 22-23 38.50951650535776 40.0 38.0 41.0 34.0 41.0 24-25 38.36815629860871 40.0 38.0 41.0 34.0 41.0 26-27 38.31316026832008 40.0 38.0 41.0 34.0 41.0 28-29 38.227741936354995 40.0 38.0 41.0 34.0 41.0 30-31 38.34101778538714 40.0 38.0 41.0 34.0 41.0 32-33 38.28998066453508 40.0 38.0 41.0 34.0 41.0 34-35 38.175397781282406 40.0 38.0 41.0 34.0 41.0 36-37 38.03952023202558 40.0 37.5 41.0 33.5 41.0 38-39 37.91274398872278 40.0 37.0 41.0 33.0 41.0 40-41 37.77649512292603 40.0 37.0 41.0 33.0 41.0 42-43 37.59110217550985 40.0 37.0 41.0 33.0 41.0 44-45 37.3117040323626 40.0 37.0 41.0 32.5 41.0 46-47 36.97549513372796 40.0 36.5 41.0 31.0 41.0 48-49 36.683273742654684 40.0 36.0 41.0 31.0 41.0 50-51 36.46357452255444 39.0 36.0 41.0 30.0 41.0 52-53 36.25921405115797 39.0 36.0 41.0 30.0 41.0 54-55 36.03527237080885 39.0 35.0 40.0 29.5 41.0 56-57 35.785756432552716 39.0 35.0 40.0 29.0 41.0 58-59 35.53282910797615 38.0 35.0 40.0 28.0 41.0 60-61 35.18714758684756 38.0 34.5 40.0 28.0 41.0 62-63 34.83806143060836 38.0 34.0 40.0 27.0 41.0 64-65 34.47646595769962 37.0 34.0 40.0 26.0 41.0 66-67 34.052487280720705 36.5 33.5 39.0 26.0 41.0 68-69 33.63587012292603 36.0 33.0 39.0 26.0 40.0 70-71 33.207314125691326 35.5 33.0 39.0 25.0 40.0 72-73 32.75399874157449 35.0 32.0 38.0 24.5 40.0 74-75 32.29641254212755 35.0 32.0 37.0 23.0 39.0 76-77 30.948456538411683 34.0 30.5 36.0 21.5 38.5 78-79 31.601204010758728 34.5 31.0 36.0 22.0 39.0 80-81 31.45238911812997 35.0 31.0 36.0 22.0 37.5 82-83 31.119946719452127 34.0 31.0 35.5 20.5 37.0 84-85 30.768680597563083 34.0 31.0 35.0 19.5 37.0 86-87 30.412425331619424 34.0 31.0 35.0 18.0 36.0 88-89 30.038891019270654 34.0 30.5 35.0 16.0 36.0 90-91 29.61692622818009 34.0 30.0 35.0 8.5 35.5 92-93 29.23796326801763 34.0 29.5 35.0 4.5 35.0 94-95 28.828160106291044 34.0 29.0 35.0 2.0 35.0 96-97 28.35997720791566 34.0 29.0 35.0 2.0 35.0 98-99 27.880544768622535 34.0 29.0 35.0 2.0 35.0 100-101 26.00765114608538 31.5 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09005566669113563 1101 2 0.11598984210420582 1101 3 0.1496661641979955 1101 4 0.08644331524958204 1101 5 -0.050162423083790486 1101 6 0.036979518402631584 1101 7 0.08336973086002075 1101 8 0.039642270914967526 1101 9 0.13890689685236168 1101 10-11 0.039154295309224096 1101 12-13 -0.06322636897747458 1101 14-15 -0.04807330745411065 1101 16-17 -0.061456511037107475 1101 18-19 -0.041258981452166665 1101 20-21 -0.004243460913265551 1101 22-23 -0.1271320831427616 1101 24-25 0.13451716647404766 1101 26-27 0.027092503215328634 1101 28-29 -0.08696091362465097 1101 30-31 0.13264635704793193 1101 32-33 0.09082708161675868 1101 34-35 -0.0051117360509707055 1101 36-37 -0.09608456444608748 1101 38-39 -0.12322849231674127 1101 40-41 -0.14374426467896484 1101 42-43 0.16848164498034635 1101 44-45 -0.0015371298132933475 1101 46-47 0.11346909731161503 1101 48-49 0.14045742868204059 1101 50-51 0.09898298860864685 1101 52-53 0.0652911713756481 1101 54-55 -0.05258059921856528 1101 56-57 -0.04169048709702139 1101 58-59 -0.18974567094318928 1101 60-61 -0.11733206564835541 1101 62-63 0.09258583065275161 1101 64-65 -0.05783085582697112 1101 66-67 -0.07541997546081092 1101 68-69 0.026154647563892297 1101 70-71 -0.18018232485826502 1101 72-73 -0.12851485676622332 1101 74-75 -0.031876975688376774 1101 76-77 -0.08701291501276032 1101 78-79 -0.2637951203002835 1101 80-81 -0.13318860828808 1101 82-83 -0.09798870008679827 1101 84-85 -0.16484794140346537 1101 86-87 -0.05703815563499859 1101 88-89 -0.10316123407706357 1101 90-91 -0.18359105162294043 1101 92-93 -0.30896761036116516 1101 94-95 -0.3503237630448446 1101 96-97 -0.3242752349401883 1101 98-99 -0.11644579771878938 1101 100-101 0.11723753320585217 1102 1 0.047682549766030036 1102 2 0.17398350142510566 1102 3 0.1394865506370806 1102 4 0.13999820659925177 1102 5 -0.0879226740190191 1102 6 0.12578999003270752 1102 7 0.11260356790720039 1102 8 0.09723799190753368 1102 9 0.17756397203491048 1102 10-11 0.1176790852577696 1102 12-13 0.004431193386920995 1102 14-15 0.10788047903149334 1102 16-17 0.10056290893851383 1102 18-19 0.24155959231930524 1102 20-21 0.1503138839017666 1102 22-23 0.18340538231195325 1102 24-25 0.08439010418060633 1102 26-27 0.1722858617416705 1102 28-29 0.0621401407656208 1102 30-31 0.23837286515372114 1102 32-33 0.2933250224322137 1102 34-35 0.21933425918254557 1102 36-37 0.20804492351554416 1102 38-39 0.05333981628443496 1102 40-41 0.13939178195004587 1102 42-43 0.1709153597200057 1102 44-45 0.08895872767796931 1102 46-47 0.21381978046346006 1102 48-49 0.250833377991718 1102 50-51 0.1338720852447537 1102 52-53 -0.014295031283225512 1102 54-55 0.01656923187709225 1102 56-57 0.017971733486632502 1102 58-59 0.2081560821799684 1102 60-61 0.18243326777861313 1102 62-63 0.2086631243209922 1102 64-65 0.2238085330450872 1102 66-67 0.34694181777804545 1102 68-69 0.38175936020707013 1102 70-71 0.18422165276940916 1102 72-73 0.11826689015377667 1102 74-75 0.3245565313040686 1102 76-77 0.07298243262001947 1102 78-79 0.15000368383651974 1102 80-81 0.4246433885869365 1102 82-83 0.3903913544249029 1102 84-85 0.30645002516093456 1102 86-87 0.2961664914653923 1102 88-89 0.2815449133386032 1102 90-91 0.3872551378981157 1102 92-93 0.33755635445055177 1102 94-95 0.13258975998218148 1102 96-97 0.3171728216723899 1102 98-99 0.6328581759587628 1102 100-101 1.215921118103509 1103 1 -0.013978457955921897 1103 2 0.0530383730015771 1103 3 -0.03551094223551843 1103 4 0.0096992137481422 1103 5 -0.1394796326976504 1103 6 -0.10439881945296037 1103 7 -0.08695661031374158 1103 8 0.03387734716553581 1103 9 -0.0026763475229003575 1103 10-11 0.004115005034897479 1103 12-13 -0.03474381192037157 1103 14-15 -0.09593660616264543 1103 16-17 -0.05811383572911666 1103 18-19 -0.06262985013463407 1103 20-21 -0.12467892031698824 1103 22-23 -0.22392011836464576 1103 24-25 -0.09163270612037167 1103 26-27 -0.05531214089179315 1103 28-29 -0.1474485470755269 1103 30-31 -0.14585189849253766 1103 32-33 -0.19588065448567704 1103 34-35 0.07397557065112181 1103 36-37 -0.08109361448403973 1103 38-39 -0.10998227730370758 1103 40-41 -0.10575806612454386 1103 42-43 -0.21909077460210113 1103 44-45 -0.40084898541809366 1103 46-47 -0.3155357510410326 1103 48-49 -0.14492970693412843 1103 50-51 0.18331166086948514 1103 52-53 -0.0015744529117256434 1103 54-55 -0.08660361925424098 1103 56-57 -0.27054132506552975 1103 58-59 -0.02921353091492307 1103 60-61 -0.1102038525543847 1103 62-63 -0.05649881006129931 1103 64-65 -0.2071077341091936 1103 66-67 -0.002492808712069916 1103 68-69 0.006077388992792976 1103 70-71 -0.11427954088372161 1103 72-73 -0.056650504882235 1103 74-75 -0.0052551020275686255 1103 76-77 -0.05097580710303973 1103 78-79 -0.054548933930089305 1103 80-81 -0.08432170829237506 1103 82-83 -0.07998253967143398 1103 84-85 -0.06876991828868739 1103 86-87 -0.18964902823932306 1103 88-89 -0.3048344169913868 1103 90-91 -0.11063138625952007 1103 92-93 0.1037667239294997 1103 94-95 0.2585683536725547 1103 96-97 0.06298624450691648 1103 98-99 -0.02477436642471531 1103 100-101 0.19744163552250882 1104 1 -0.04127760255543933 1104 2 -0.07715998019436654 1104 3 -0.06274869445587683 1104 4 -0.15145578154510275 1104 5 -0.11979686386413846 1104 6 -0.00915319412506932 1104 7 -0.08339585232249647 1104 8 -0.09788930226165604 1104 9 -0.13434997819378935 1104 10-11 0.01277319087149209 1104 12-13 -0.10256936826555574 1104 14-15 -0.17680897326675193 1104 16-17 -0.21774704416926483 1104 18-19 -0.1591423909233569 1104 20-21 -0.3425567532032048 1104 22-23 -0.24036676466641183 1104 24-25 -0.0041578676939408865 1104 26-27 -0.28974824258733634 1104 28-29 -0.3002541788031081 1104 30-31 -0.13223953369529795 1104 32-33 -0.18064790295365896 1104 34-35 -0.14144098594334764 1104 36-37 -0.20217426918725323 1104 38-39 -0.25988479364710315 1104 40-41 -0.26948875077980006 1104 42-43 -0.167663494805403 1104 44-45 -0.06118448546832411 1104 46-47 -0.09909970887749608 1104 48-49 -0.1128670718243896 1104 50-51 -0.28315693785877016 1104 52-53 -0.35644844970444467 1104 54-55 -0.38120425252593293 1104 56-57 -0.1681695328173447 1104 58-59 -0.17163175812625298 1104 60-61 -0.06585995105009346 1104 62-63 -0.35559131907481856 1104 64-65 -0.4475695908379862 1104 66-67 -0.4406176622075222 1104 68-69 -0.17598543215446227 1104 70-71 -0.3776844738544014 1104 72-73 -0.1926085794683985 1104 74-75 -0.3359665466832702 1104 76-77 -0.3408957365576164 1104 78-79 -0.24204498063295077 1104 80-81 -0.2847743375605418 1104 82-83 -0.15938241986327384 1104 84-85 -0.27516458570942604 1104 86-87 -0.3860069718550747 1104 88-89 -0.3739027039207592 1104 90-91 -0.4567141803941297 1104 92-93 -0.5419992483211438 1104 94-95 -0.42287589274529225 1104 96-97 -0.15382466324452437 1104 98-99 -0.3641560893553262 1104 100-101 0.3152043111884275 1105 1 -0.19517395502267476 1105 2 -8.49803195144716E-4 1105 3 -0.17199421478981947 1105 4 -0.06073046755833644 1105 5 -0.1797177519590747 1105 6 -0.1713763480287156 1105 7 -0.16507616002041914 1105 8 -0.08397727324180693 1105 9 -0.0035109180578061228 1105 10-11 -0.1541629545194212 1105 12-13 -0.2239405197630262 1105 14-15 -0.27256718978546246 1105 16-17 -0.09199466078641194 1105 18-19 -0.13240657729205196 1105 20-21 -0.21443921551568224 1105 22-23 -0.21847883378907085 1105 24-25 -0.2632046968377537 1105 26-27 -0.06787033741225201 1105 28-29 -0.18155350981533758 1105 30-31 -0.34323864049636654 1105 32-33 -0.28074451426629565 1105 34-35 -0.1435518519817336 1105 36-37 -0.5003067542854112 1105 38-39 -0.5178998868861271 1105 40-41 -0.47693511327273796 1105 42-43 -0.600913280619956 1105 44-45 -0.5272648004576581 1105 46-47 -0.5248803465121554 1105 48-49 -0.45218787606798827 1105 50-51 -0.36951924850971096 1105 52-53 -0.40746833705200913 1105 54-55 -0.48268805645965784 1105 56-57 -0.42046997577110545 1105 58-59 -0.47438700339139217 1105 60-61 -0.35583424879521885 1105 62-63 -0.43243103543858297 1105 64-65 -0.34131719834085317 1105 66-67 -0.20692583406628984 1105 68-69 -0.43951192735123357 1105 70-71 -0.6591733817819971 1105 72-73 -0.4527226901886081 1105 74-75 -0.2914998229004837 1105 76-77 -0.24508192296276476 1105 78-79 -0.3378423885332005 1105 80-81 -0.370657237358202 1105 82-83 -0.3036395153261857 1105 84-85 -0.3819428718611988 1105 86-87 -0.17173675183648385 1105 88-89 -0.07489798272642645 1105 90-91 -0.2779267435104842 1105 92-93 -0.4121600241282124 1105 94-95 0.06280102444240754 1105 96-97 -0.26871374252048597 1105 98-99 -0.2184999527141187 1105 100-101 -0.057744521272972804 1106 1 -0.2589717582130504 1106 2 -0.11363871914540624 1106 3 -0.04528894534999495 1106 4 -0.08880511392360546 1106 5 -0.12150390980033166 1106 6 -0.290291169235509 1106 7 -0.33794346433177935 1106 8 -0.09925493901061344 1106 9 -0.25638919028800444 1106 10-11 -0.2979684997701497 1106 12-13 -0.373760678152685 1106 14-15 -0.4131307472621941 1106 16-17 -0.18745426497357442 1106 18-19 -0.22496774763350658 1106 20-21 -0.22924429301693294 1106 22-23 -0.32304503646975746 1106 24-25 -0.1350123066379112 1106 26-27 -0.3167049960357815 1106 28-29 -0.28162447870193574 1106 30-31 -0.10352993551725831 1106 32-33 -0.1545134460232589 1106 34-35 -0.3616311758418007 1106 36-37 -0.10504231453639079 1106 38-39 -0.11172881961085324 1106 40-41 -0.25639445939122396 1106 42-43 -0.48965503451920966 1106 44-45 -0.4675509341049775 1106 46-47 -0.18506196234466898 1106 48-49 -0.12681488618105874 1106 50-51 0.05334241054931255 1106 52-53 -0.035415562007244716 1106 54-55 0.016252249029143684 1106 56-57 -0.1517204798114875 1106 58-59 -0.29753576385527225 1106 60-61 0.028360657574239667 1106 62-63 0.2702190073040427 1106 64-65 0.30656583203059995 1106 66-67 0.12915055426255861 1106 68-69 0.2454071842473411 1106 70-71 -0.18431572278750252 1106 72-73 0.1257076070742542 1106 74-75 0.16070263197782708 1106 76-77 0.4310418854857616 1106 78-79 0.5304151640079731 1106 80-81 0.6325557176894563 1106 82-83 0.7295513425908737 1106 84-85 0.6345170710411061 1106 86-87 0.5186388993836957 1106 88-89 0.5698688683518149 1106 90-91 0.23198309374495807 1106 92-93 0.23221165928603682 1106 94-95 -0.5323214775334328 1106 96-97 -0.04163427349678983 1106 98-99 0.5452091393680121 1106 100-101 1.0033145280665323 1107 1 -0.09850118997569979 1107 2 -0.12168455776117781 1107 3 -0.24253593701376985 1107 4 -0.15064333307355326 1107 5 -0.34802684681473295 1107 6 -0.24962993728729543 1107 7 -0.2068479979068627 1107 8 -0.12955077939703585 1107 9 -0.11555160148984811 1107 10-11 -0.10913210084954272 1107 12-13 -0.16344096938937724 1107 14-15 -0.41798410193777613 1107 16-17 -0.3878689514620888 1107 18-19 -0.25194361720026137 1107 20-21 -0.25625911690797665 1107 22-23 -0.11110341129534618 1107 24-25 -0.2227445275301747 1107 26-27 -0.3033737638750864 1107 28-29 -0.2716043641053929 1107 30-31 -0.2922618660227414 1107 32-33 -0.4925820906252554 1107 34-35 -0.5149571668752273 1107 36-37 -0.3625427926121816 1107 38-39 -0.4458294350891805 1107 40-41 -0.5158727547499922 1107 42-43 -0.599001593435851 1107 44-45 -0.6189203556686778 1107 46-47 -0.7230601350327319 1107 48-49 -0.8680519338682373 1107 50-51 -0.8091452834255151 1107 52-53 -0.7444586313246901 1107 54-55 -0.7566174806278454 1107 56-57 -0.7718467626482166 1107 58-59 -0.8536282201733059 1107 60-61 -1.0598266206517124 1107 62-63 -1.2754230661992239 1107 64-65 -1.0845231892242992 1107 66-67 -0.8333824683805346 1107 68-69 -0.7936763975673671 1107 70-71 -0.7756901730003349 1107 72-73 -0.6157401480942823 1107 74-75 -0.6050490802417983 1107 76-77 -0.8283868316421028 1107 78-79 -0.9307491453318626 1107 80-81 -0.9233968571913778 1107 82-83 -0.7129219736969894 1107 84-85 -0.7337677346476426 1107 86-87 -0.7850472420842429 1107 88-89 -0.7213164763231283 1107 90-91 -0.6565384834093955 1107 92-93 -0.4529240788275821 1107 94-95 -0.5725365054034803 1107 96-97 -0.6993798096853574 1107 98-99 -0.5148672819336326 1107 100-101 0.013787929345649985 1108 1 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88-89 -0.4798209819690449 2316 90-91 -0.3478423492377516 2316 92-93 -0.33999390446146194 2316 94-95 -0.45922624139955204 2316 96-97 -0.6873634341567971 2316 98-99 -0.7099126551341435 2316 100-101 -0.8769442183191742 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 4.0 5 11.0 6 27.0 7 79.0 8 156.0 9 320.0 10 510.0 11 826.0 12 1296.0 13 1861.0 14 2406.0 15 3275.0 16 4669.0 17 6860.0 18 6074.0 19 3666.0 20 3234.0 21 3400.0 22 3496.0 23 3775.0 24 4455.0 25 5204.0 26 6265.0 27 7839.0 28 9688.0 29 12448.0 30 15516.0 31 19646.0 32 25222.0 33 33344.0 34 46677.0 35 67369.0 36 107885.0 37 170031.0 38 147324.0 39 15745.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.325970806568186 19.886117246550082 14.873925172411465 21.91398677447027 2 15.751247623574145 21.212706316972003 39.454205193570694 23.581840865883166 3 20.23202557898375 24.72671102661597 30.06259721742136 24.978666176978916 4 13.202665917732459 16.98914945558244 37.62408723643277 32.18409739025233 5 13.707604771738 38.101244795345494 34.18737161402026 14.003778818896256 6 32.12941259073626 37.11113031455236 16.744620636017974 14.014836458693397 7 28.389647424818527 33.361643406498445 20.52259764949879 17.726111519184236 8 26.50903041825095 35.18892585551331 19.38974464223989 18.91229908399585 9 26.092615796750778 17.389496197718632 20.504639431386103 36.013248574144484 10-11 24.996016786208088 27.037650146906326 27.35542959298306 20.61090347390252 12-13 26.25161190140227 24.2839782064179 27.904426905756935 21.559982986422895 14-15 22.609126555478742 25.88339580452817 26.69023288973384 24.817244750259245 16-17 21.799318937953682 29.31496824230902 27.116369253370205 21.769343566367095 18-19 21.788111929657795 27.946498012443833 29.42589061959903 20.839499438299345 20-21 22.610341773245764 26.66829145782924 29.524053210335293 21.197313558589702 22-23 22.304895206872246 26.667764872360593 29.599974075144274 21.427365845622884 24-25 22.653310055130454 26.746439069574013 29.560077071915337 21.0401738033802 26-27 22.24711940226964 26.814217902610242 29.92081436508028 21.01784833003984 28-29 21.78169960234139 27.60992998777993 29.54218624465794 21.06618416522074 30-31 22.127630271344625 26.834573755617008 29.50157168164535 21.536224291393015 32-33 21.962900751814725 27.107862729001038 29.522230383684757 21.407006135499483 34-35 22.565716276356724 26.82296167473211 29.406919720013825 21.204402328897338 36-37 22.484229173867956 26.725879277566538 29.633152760974767 21.156738787590736 38-39 22.034463575872795 27.249975695644657 29.703567879363984 21.011992849118563 40-41 22.119326283269963 27.307631027480124 29.233076067231245 21.339966622018665 42-43 22.0258299667858 27.390811435300506 28.885889399882377 21.69746919803132 44-45 22.485309367438646 27.090039535084685 28.857641289319048 21.56700980815762 46-47 22.769332764431386 27.664297442101628 28.492940935015554 21.073428858451436 48-49 22.737197005703422 27.70649250345662 28.267585551330797 21.28872493950916 50-51 22.29992114586934 27.960540528862772 28.480046124265467 21.25949220100242 52-53 22.752927324576564 28.317679528171446 27.629191151054265 21.300201996197718 54-55 22.458101991876944 27.815186981507082 27.936506221914968 21.790204804701002 56-57 22.619658442792947 28.02359682855168 27.956152242481853 21.400592486173522 58-59 23.089340109747667 27.664770026788798 27.755236238333907 21.490653625129625 60-61 22.54998595748358 27.6717237729001 28.665771906325617 21.112518363290704 62-63 22.243278495506395 27.833820320601454 28.585972606291048 21.336928577601107 64-65 22.234501922744556 28.134451693743518 28.09644238247494 21.534604001036985 66-67 22.33347465865883 27.786089267196683 27.899374567922568 21.981061506221916 68-69 22.26386968544763 27.87453011579675 27.98484488420325 21.87675531455237 70-71 22.581109034739026 27.95486951261666 27.882091470791565 21.58192998185275 72-73 22.40142207675596 27.511487199013786 28.224888503619326 21.862202220610932 74-75 22.198788022813687 28.309105491704113 27.734915096785347 21.757191388696853 76-77 22.30518708952644 27.808233235395786 27.769616315243688 22.11696335983408 78-79 22.435417926892498 27.787709557552713 27.62790842118908 22.148964094365713 80-81 22.456144141030073 27.852116099204977 27.838006070687864 21.85373368907708 82-83 22.32462334898718 27.765775594580656 28.103472021560723 21.806129034871443 84-85 21.982746398523357 27.86710487051697 28.00375101775725 22.146397713202422 86-87 22.089283399585206 27.864875885758728 27.734847584687177 22.31099312996889 88-89 22.001180111475975 27.723100479605943 27.520024088316624 22.75569532060145 90-91 22.250775461259344 26.998272851631338 27.99215963766345 22.758792049445866 92-93 22.438360788549826 27.194504455846612 28.072306238185256 22.29482851741831 94-95 21.947710529726926 27.528530612685792 27.98977326736951 22.533985590217767 96-97 21.492611475976496 27.532176265986863 28.42077050207397 22.554441755962667 98-99 21.614162437028007 28.04348325090095 27.577446607985074 22.764907704085974 100-101 21.36571387286945 28.059713182801232 27.731626933727117 22.8429460106022 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 165.0 1 116.0 2 105.5 3 154.5 4 132.0 5 126.5 6 216.5 7 477.0 8 818.5 9 993.0 10 950.0 11 881.0 12 913.5 13 962.0 14 1041.0 15 1156.0 16 1215.0 17 1218.5 18 1258.5 19 1334.5 20 1464.0 21 1683.0 22 1946.0 23 2319.0 24 2855.5 25 3552.0 26 4477.0 27 5579.0 28 6482.0 29 7609.5 30 9105.0 31 10313.5 32 11424.0 33 12863.0 34 14549.5 35 15990.5 36 17666.5 37 19293.0 38 20506.0 39 21164.0 40 21722.0 41 23167.0 42 24615.0 43 26188.5 44 27808.0 45 28619.5 46 28793.5 47 31116.5 48 35766.5 49 34375.5 50 29487.5 51 26834.0 52 24769.0 53 23755.0 54 22810.0 55 20869.5 56 18204.0 57 16179.5 58 14655.0 59 12882.5 60 10640.5 61 8229.5 62 6364.5 63 4914.0 64 3631.0 65 2674.5 66 1894.0 67 1441.5 68 1045.5 69 650.0 70 476.0 71 337.0 72 221.0 73 164.0 74 115.5 75 74.0 76 52.0 77 29.5 78 14.0 79 12.0 80 9.0 81 3.0 82 0.0 83 0.5 84 0.5 85 0.0 86 0.5 87 1.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.050364025233321806 2 0.0 3 0.0 4 0.0 5 0.022278992395437264 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0031055565157276184 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.101451780159005E-4 24-25 1.3502419633598341E-4 26-27 8.776572761838923E-4 28-29 0.0031055565157276184 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.01505519789146215 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.3502419633598341E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.7004839267196683E-4 84-85 0.0012152177670238507 86-87 0.0 88-89 0.0 90-91 0.010936959903214655 92-93 0.0010801935706878673 94-95 0.0 96-97 0.0 98-99 1.3502419633598341E-4 100-101 0.013704955928102317 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 740608.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.44293708448563 #Duplication Level Percentage of deduplicated Percentage of total 1 81.7326538156368 51.85359613773489 2 10.792424906667149 13.69406268685439 3 3.151367258106627 5.997959840585002 4 1.4422809786949253 3.6601016555797 5 0.7496486129710949 2.3779954894098543 6 0.4529710986405837 1.7242690147286934 7 0.3252888167007315 1.4446094552561901 8 0.22512777203554496 1.1426213661777203 9 0.1687698203416827 0.9636527784327588 >10 0.886515085685243 10.056409768125532 >50 0.050554651716705416 2.1655522661093585 >100 0.018984278759641846 2.014355946203149 >500 0.0021330650270766018 0.9662412796129536 >1k 0.001279839016245961 1.9385723151898544 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3983 0.5378013740062219 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3409 0.46029748530936737 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2634 0.3556537331489803 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1974 0.26653776356723125 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1164 0.1571681645350847 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1161 0.15676309194607674 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 959 0.12948820428620808 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 922 0.1244923090217767 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 840 0.11342032492222605 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 796 0.10747926028344279 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 1.3502419633598341E-4 0.0 0.0 0.0 0.0 8 1.3502419633598341E-4 0.0 0.0 0.0 0.0 9 1.3502419633598341E-4 0.0 0.0 0.0 0.0 10-11 1.3502419633598341E-4 0.0 0.0 0.0 0.0 12-13 1.3502419633598341E-4 0.0 0.0 0.0 0.0 14-15 2.7004839267196683E-4 0.0 0.0 0.0 0.0 16-17 3.375604908399585E-4 0.0 0.0 6.751209816799171E-5 0.0 18-19 4.0507258900795024E-4 0.0 0.0 1.3502419633598341E-4 0.0 20-21 4.0507258900795024E-4 0.0 0.0 1.3502419633598341E-4 0.0 22-23 4.0507258900795024E-4 0.0 0.0 2.0253629450397512E-4 0.0 24-25 4.0507258900795024E-4 0.0 0.0 2.7004839267196683E-4 0.0 26-27 4.0507258900795024E-4 0.0 0.0 8.101451780159005E-4 0.0 28-29 4.0507258900795024E-4 0.0 0.0 0.002227899239543726 0.0 30-31 4.0507258900795024E-4 0.0 0.0 0.004455798479087452 0.0 32-33 4.0507258900795024E-4 0.0 0.0 0.007831403387487038 0.0 34-35 4.0507258900795024E-4 0.0 0.0 0.011544568786726581 0.0 36-37 4.0507258900795024E-4 0.0 0.0 0.01633792775665399 0.0 38-39 4.0507258900795024E-4 0.0 0.0 0.021536359315589355 0.0 40-41 4.72584687175942E-4 0.0 0.0 0.026667278776356725 0.0 42-43 5.400967853439337E-4 0.0 0.0 0.038616920152091255 0.0 44-45 5.400967853439337E-4 0.0 0.0 0.05515738420324923 0.0 46-47 5.400967853439337E-4 0.0 0.0 0.07392574749395092 0.0 48-49 5.400967853439337E-4 0.0 0.0 0.09647478828206014 0.0 50-51 5.400967853439337E-4 0.0 0.0 0.1261801114759765 0.0 52-53 5.400967853439337E-4 0.0 0.0 0.187278560318009 0.0 54-55 5.400967853439337E-4 0.0 0.0 0.23291673867957138 0.0 56-57 5.400967853439337E-4 0.0 0.0 0.3230453897338403 0.0 58-59 5.400967853439337E-4 0.0 0.0 0.4216130530591082 0.0 60-61 5.400967853439337E-4 0.0 0.0 0.5222735914275838 0.0 62-63 5.400967853439337E-4 0.0 0.0 0.675863614759765 0.0 64-65 5.400967853439337E-4 0.0 0.0 0.7522873098859315 0.0 66-67 6.75120981679917E-4 0.0 0.0 0.9611022295195298 0.0 68-69 6.75120981679917E-4 0.0 0.0 1.2110995290356033 6.751209816799171E-5 70-71 6.75120981679917E-4 0.0 0.0 1.3929096094020048 1.3502419633598341E-4 72-73 8.776572761838922E-4 0.0 0.0 1.7279721526097478 2.7004839267196683E-4 74-75 9.451693743518838E-4 0.0 0.0 2.072351365364673 2.7004839267196683E-4 76-77 9.451693743518838E-4 0.0 0.0 2.277318095402696 2.7004839267196683E-4 78-79 9.451693743518838E-4 0.0 0.0 2.5307585119253373 2.7004839267196683E-4 80-81 9.451693743518838E-4 0.0 0.0 2.9884905375043207 2.7004839267196683E-4 82-83 9.451693743518838E-4 0.0 0.0 3.3647354605945385 2.7004839267196683E-4 84-85 9.451693743518838E-4 0.0 0.0 3.919954955928102 2.7004839267196683E-4 86-87 9.451693743518838E-4 0.0 0.0 4.583126296232285 2.7004839267196683E-4 88-89 9.451693743518838E-4 0.0 0.0 5.520734315589353 2.7004839267196683E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAGCG 55 9.483663E-5 25.906992 66-67 TATTCCG 95 0.0012252743 25.003038 5 ACGGATC 95 0.0012257568 25.00135 8 GTACATG 2480 0.0 24.147411 1 GAGTACT 2925 0.0 23.54828 12-13 ACATGGG 2510 0.0 23.468987 3 GTGTAGG 265 3.092282E-11 23.31574 1 TACATGG 2585 0.0 23.155619 2 GTACTTT 3065 0.0 22.314669 14-15 AGTACTT 3060 0.0 21.966057 12-13 CATGGGG 1300 0.0 21.560314 4 AGAGTAC 4430 0.0 21.49947 10-11 TAGACCC 160 4.981544E-5 20.783777 4 GTAAGGT 210 8.589159E-7 20.359617 4 TAAGGTG 265 1.8493665E-8 19.719378 5 TAGCGAC 85 7.960787E-5 19.55724 68-69 AGGCGTG 250 2.168199E-7 19.001028 7 GCGACGA 75 7.7267055E-4 18.99846 70-71 ACTTTTT 3715 0.0 18.603384 16-17 TACTTTT 3600 0.0 18.60266 14-15 >>END_MODULE