FastQCFastQC Report
Thu 26 May 2016
SRR937352_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937352_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047609
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38350.36607169277850804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27740.26479344870080346No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25530.24369779182882162No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT22450.21429750985338994No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20250.19329730844236734No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18630.17783352376697795No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16680.15921970887993517No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC16040.15311055937854676No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT12090.11540565229966523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC2151.8189894E-1228.723523
GTACATG35100.027.0744461
GTATAGA3450.026.168041
TACATGG34700.025.735962
GTATCAA61200.025.1553461
ACATGGG36150.023.259363
GGTATCA45550.022.4278071
GAGTACT32250.021.87040112-13
GTGTAGG3500.021.7214411
TCAACGC70400.021.6624074
ATCAACG70450.021.6449623
CAACGCA70450.021.5795975
AACGCAG71100.021.3823176
AGAGTAC51300.021.15678210-11
TATCAAC72600.021.1338142
CATGGGG20350.020.544364
AGTACTT34800.020.54079612-13
GTACTTT34750.020.36581814-15
ACCGCGT600.00476559219.79108210-11
GTGTAGC5700.019.1729831
Produced by FastQC (version 0.11.5)