##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937352_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1047609 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.63030481792348 33.0 31.0 34.0 30.0 34.0 2 31.737572892176374 33.0 31.0 34.0 30.0 34.0 3 31.78487966407314 33.0 31.0 34.0 30.0 34.0 4 35.38082242516053 37.0 35.0 37.0 33.0 37.0 5 35.38629965951037 37.0 35.0 37.0 33.0 37.0 6 35.40530388723273 37.0 35.0 37.0 33.0 37.0 7 35.269280809920495 37.0 35.0 37.0 33.0 37.0 8 35.28292139529157 37.0 35.0 37.0 33.0 37.0 9 36.897208786866095 39.0 37.0 39.0 33.0 39.0 10-11 36.78435656814708 39.0 37.0 39.0 33.0 39.0 12-13 36.627757111670476 39.0 37.0 39.0 32.0 39.0 14-15 37.79715284996597 40.0 38.0 41.0 32.5 41.0 16-17 37.739276294877186 40.0 38.0 41.0 32.5 41.0 18-19 37.69419506705269 40.0 37.5 41.0 32.0 41.0 20-21 37.57498121913805 40.0 37.0 41.0 32.0 41.0 22-23 37.410909509177564 40.0 37.0 41.0 32.0 41.0 24-25 37.346091910245136 40.0 37.0 41.0 32.0 41.0 26-27 37.359711972692104 40.0 37.0 41.0 32.0 41.0 28-29 37.271915380642966 40.0 37.0 41.0 31.5 41.0 30-31 37.085682253588885 39.5 36.5 41.0 31.0 41.0 32-33 36.86193226671401 39.0 36.0 41.0 30.5 41.0 34-35 36.91606601317858 39.0 36.0 41.0 30.5 41.0 36-37 36.8115656700162 39.5 36.0 41.0 30.0 41.0 38-39 36.7010034278056 39.0 36.0 41.0 30.0 41.0 40-41 36.50578889642987 39.0 35.5 41.0 30.0 41.0 42-43 36.25304574512056 39.0 35.0 41.0 30.0 41.0 44-45 36.04856057937647 39.0 35.0 41.0 29.0 41.0 46-47 35.74252607604555 39.0 35.0 41.0 27.5 41.0 48-49 35.4371850566385 39.0 35.0 40.0 26.5 41.0 50-51 34.458568511725275 38.0 34.0 39.5 25.0 40.5 52-53 34.583715871093126 38.0 34.0 39.5 25.5 40.5 54-55 34.96510816535559 38.0 34.5 40.0 26.0 41.0 56-57 34.73943952371543 38.0 34.0 40.0 24.5 41.0 58-59 34.46250843587636 38.0 34.0 40.0 24.0 41.0 60-61 34.14109701233953 38.0 34.0 40.0 23.0 41.0 62-63 33.82783414422748 37.5 33.0 40.0 22.0 41.0 64-65 33.39212673812463 37.0 33.0 40.0 21.5 41.0 66-67 33.03058345241402 36.0 32.0 39.0 20.0 41.0 68-69 32.539153443698936 36.0 32.0 39.0 18.5 40.5 70-71 32.0318654192547 35.0 31.0 38.5 15.5 40.0 72-73 31.56551871929317 35.0 31.0 38.0 12.0 40.0 74-75 31.065893381977435 35.0 30.5 37.0 8.0 39.0 76-77 30.576684621838872 34.5 30.0 36.5 7.0 39.0 78-79 30.04620855681843 34.0 29.5 36.0 2.0 39.0 80-81 29.528631388237407 34.0 29.0 36.0 2.0 37.5 82-83 29.033239977892514 34.0 29.0 35.0 2.0 37.0 84-85 28.494073170429044 34.0 28.0 35.0 2.0 36.5 86-87 28.03785477215259 34.0 27.0 35.0 2.0 36.0 88-89 27.578104044543338 33.0 26.0 35.0 2.0 36.0 90-91 27.046499695974354 33.0 25.5 35.0 2.0 35.0 92-93 26.510584101511157 32.5 25.0 35.0 2.0 35.0 94-95 25.942597381274883 32.0 23.5 34.5 2.0 35.0 96-97 25.198418016645526 32.0 19.5 34.0 2.0 35.0 98-99 24.249387414579296 31.5 8.5 34.0 2.0 35.0 100-101 21.719402945182793 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.1481157467247769 1101 2 -0.18323773210485683 1101 3 -0.19636021849953522 1101 4 -0.21603286905489938 1101 5 -0.2223531917691588 1101 6 -0.24164538577434058 1101 7 -0.29098562454633026 1101 8 -0.3767634555667314 1101 9 -0.4090560376291634 1101 10-11 -0.4573484130090151 1101 12-13 -0.3061020022246339 1101 14-15 -0.4654715762139645 1101 16-17 -0.5365828447389873 1101 18-19 -0.18978052259195266 1101 20-21 -0.22640647766437638 1101 22-23 -0.2775317021115171 1101 24-25 -0.2609563420976855 1101 26-27 -0.39986327453180337 1101 28-29 -0.4160540306577829 1101 30-31 -0.36546266078778444 1101 32-33 -0.44651972971432485 1101 34-35 -0.35972245197040564 1101 36-37 -0.292327476527916 1101 38-39 -0.429396796822779 1101 40-41 -0.45813159095512646 1101 42-43 -0.37006081471715646 1101 44-45 -0.45595179925758345 1101 46-47 -0.5318822998365746 1101 48-49 -0.2930707408323556 1101 50-51 -0.35330083857575545 1101 52-53 -0.21337666969150604 1101 54-55 -0.30535207454362734 1101 56-57 -0.34196687862268504 1101 58-59 -0.26818123717261244 1101 60-61 -0.1437159374620265 1101 62-63 -0.4891033541934604 1101 64-65 -0.5780116737596117 1101 66-67 -0.5420832125926935 1101 68-69 -0.509781104933829 1101 70-71 -0.6153910123373052 1101 72-73 -0.3464032719556336 1101 74-75 -0.561414150257388 1101 76-77 -0.5694546318721514 1101 78-79 -0.7815135677278917 1101 80-81 -0.7034670616516223 1101 82-83 -0.6544070280305121 1101 84-85 -0.7228542912276836 1101 86-87 -0.6823706971756103 1101 88-89 -0.9654859553690507 1101 90-91 -0.807517817291572 1101 92-93 -0.8628472154478786 1101 94-95 -0.9082957715139628 1101 96-97 -0.6896565498448233 1101 98-99 -0.5660568146746847 1101 100-101 -0.11913481909615342 1102 1 -0.17919763888451357 1102 2 -0.2812040372567921 1102 3 -0.1708713361244243 1102 4 -0.2494736797412216 1102 5 -0.06107784037404684 1102 6 -0.17453136207349473 1102 7 -0.19027794898835992 1102 8 -0.28668766309572646 1102 9 -0.22182769710740757 1102 10-11 -0.31327432342594363 1102 12-13 -0.34619942974720885 1102 14-15 -0.31861877831326524 1102 16-17 -0.2899962975536283 1102 18-19 -0.3495892268617382 1102 20-21 -0.42457133675738135 1102 22-23 -0.4810079663662279 1102 24-25 -0.19803602887797922 1102 26-27 -0.36555531702024524 1102 28-29 -0.4033280839498019 1102 30-31 -0.375114336457429 1102 32-33 -0.46214756495294296 1102 34-35 -0.28495625010839376 1102 36-37 -0.27793981827797865 1102 38-39 -0.14403395607072156 1102 40-41 -0.27882830940633596 1102 42-43 -0.4248526296886155 1102 44-45 -0.07783704973564198 1102 46-47 -0.021794782563425485 1102 48-49 -0.20504923140365605 1102 50-51 -0.18257515707162497 1102 52-53 -0.22025975666441866 1102 54-55 -0.13805450185683554 1102 56-57 -0.05212732695678568 1102 58-59 -0.1486423429331154 1102 60-61 -0.035303740876173606 1102 62-63 -0.37419819946794775 1102 64-65 -0.07282972995491122 1102 66-67 -0.22034522004631896 1102 68-69 -0.19135920747000768 1102 70-71 -0.145115130534478 1102 72-73 -0.1214071104325285 1102 74-75 -0.29127017247867926 1102 76-77 -0.19971299626225303 1102 78-79 -0.10578076759389532 1102 80-81 -0.23538888234589805 1102 82-83 -0.35789829724034306 1102 84-85 -0.00910905442855281 1102 86-87 -0.06884948784601619 1102 88-89 -0.3959875396131487 1102 90-91 -0.38049389311195725 1102 92-93 -0.44641539377654027 1102 94-95 -0.40685599372687875 1102 96-97 -0.3358791396302898 1102 98-99 -0.400638350902927 1102 100-101 -0.1255698511299883 1103 1 -0.13253866325778674 1103 2 -0.19918344541295596 1103 3 -0.15638539974031218 1103 4 -0.13366401466770128 1103 5 -0.12062846389764559 1103 6 -0.30130648155929407 1103 7 -0.20471581637285396 1103 8 -0.1787704699590691 1103 9 -0.159928559602605 1103 10-11 -0.12045468746850219 1103 12-13 -0.23748967036689095 1103 14-15 -0.31665538857896536 1103 16-17 -0.34682816134898076 1103 18-19 -0.39604562599009085 1103 20-21 -0.38430782707948197 1103 22-23 -0.4230573538298259 1103 24-25 -0.4304370525187693 1103 26-27 -0.5095666063681605 1103 28-29 -0.5798694965374693 1103 30-31 -0.5389455043967715 1103 32-33 -0.43970849813245394 1103 34-35 -0.47263879785693774 1103 36-37 -0.39779757981911956 1103 38-39 -0.6304825039291373 1103 40-41 -0.4165225310547811 1103 42-43 -0.30351216840934114 1103 44-45 -0.5524039709391104 1103 46-47 -0.2075042123277484 1103 48-49 -0.18162303334106866 1103 50-51 -0.2414814271003749 1103 52-53 -0.21162507648349305 1103 54-55 -0.0961200221923093 1103 56-57 -0.15312601764879474 1103 58-59 -0.2839783223504284 1103 60-61 -0.23896865874685602 1103 62-63 -0.023595505772767922 1103 64-65 -0.2533454187607944 1103 66-67 -0.34020183708747 1103 68-69 -0.27554290429653605 1103 70-71 -0.21755454070495972 1103 72-73 -0.30890768268252344 1103 74-75 -0.2787539205788079 1103 76-77 -0.28732275381137384 1103 78-79 -0.1199542554805042 1103 80-81 -0.17175754410494903 1103 82-83 -0.34013966313241184 1103 84-85 -0.4485215139132528 1103 86-87 -0.20093852078930752 1103 88-89 -0.12046633431731735 1103 90-91 0.04434564798932428 1103 92-93 -0.07019936253626469 1103 94-95 -0.498436593994235 1103 96-97 -0.585368120384377 1103 98-99 -0.4513700297470713 1103 100-101 0.09477500960725393 1104 1 -0.06032776378260962 1104 2 -0.12617614464679505 1104 3 -0.10319611388168681 1104 4 -0.09267839680175172 1104 5 -0.13871583059875547 1104 6 -0.14411736313618917 1104 7 0.01458105546968369 1104 8 -0.03380450555932413 1104 9 -0.04024247233982692 1104 10-11 -0.08736993333890553 1104 12-13 -0.09117141152311348 1104 14-15 -0.03790032221098727 1104 16-17 -0.16397395583052798 1104 18-19 -0.04498493638667611 1104 20-21 -0.23761626640720124 1104 22-23 -0.3178493873631183 1104 24-25 -0.17718974159100043 1104 26-27 -0.1264889249459955 1104 28-29 -0.2884544642917035 1104 30-31 -0.36575781350584435 1104 32-33 -0.38319612233785705 1104 34-35 -0.29633767228606445 1104 36-37 -0.44886433174318796 1104 38-39 -0.30367596845878353 1104 40-41 -0.2633192080370179 1104 42-43 -0.283039025933256 1104 44-45 -0.26555805203563665 1104 46-47 -0.06585141442242559 1104 48-49 -0.2302466339553817 1104 50-51 -0.17586799242044293 1104 52-53 -0.3046392983990671 1104 54-55 -0.31516032796421456 1104 56-57 -0.293126291896634 1104 58-59 -0.22867155449111465 1104 60-61 -0.2816657996409617 1104 62-63 0.06318914038978818 1104 64-65 0.10842230342326076 1104 66-67 -0.09072795991585991 1104 68-69 -0.09658211596390487 1104 70-71 -0.05485213858256799 1104 72-73 0.07161967426114302 1104 74-75 -0.08869655947812305 1104 76-77 -0.15118450258880856 1104 78-79 0.014237377362260162 1104 80-81 0.1208434296192209 1104 82-83 0.26119689814610325 1104 84-85 0.3106030003793876 1104 86-87 0.49214494870567194 1104 88-89 0.2587632168977443 1104 90-91 0.5087067804308241 1104 92-93 0.5372586991954797 1104 94-95 0.5232224899602471 1104 96-97 0.6271838784493475 1104 98-99 0.5188742601242318 1104 100-101 1.0090801377531662 1105 1 -0.1568031129698504 1105 2 -0.17625236205173067 1105 3 -0.26156336977863504 1105 4 -0.0817792844662435 1105 5 -0.044530518041796086 1105 6 -0.1471538757951194 1105 7 -0.09522308477783525 1105 8 -0.1618717699378891 1105 9 -0.03753210363315418 1105 10-11 -0.14641370363796824 1105 12-13 -0.16066654911978162 1105 14-15 -0.23757071063625546 1105 16-17 -0.32521699539844917 1105 18-19 -0.10746680751577031 1105 20-21 -0.08959330324881876 1105 22-23 0.040343597074283366 1105 24-25 -0.0633753428041075 1105 26-27 -0.10101384895117604 1105 28-29 -0.16615704256273034 1105 30-31 -0.0930189962368928 1105 32-33 -0.011852882538171627 1105 34-35 -0.10570325200928465 1105 36-37 0.0074642432935903 1105 38-39 0.13360622150414514 1105 40-41 0.17673989149640335 1105 42-43 0.18326880428632109 1105 44-45 0.3434337742158604 1105 46-47 0.3224965305660916 1105 48-49 0.38282620907799725 1105 50-51 0.17166275578471613 1105 52-53 0.35632515987629176 1105 54-55 0.29315976358102347 1105 56-57 0.2981499665574532 1105 58-59 0.6371797210550625 1105 60-61 0.8527535399456454 1105 62-63 0.6253354956139674 1105 64-65 0.5850083541152173 1105 66-67 0.4785141349592337 1105 68-69 0.31934982866471984 1105 70-71 0.1263465997651636 1105 72-73 0.6530640514878563 1105 74-75 0.48729685578794246 1105 76-77 0.3870427550794453 1105 78-79 0.6168623675802856 1105 80-81 0.5899825267346728 1105 82-83 0.6271749293518951 1105 84-85 0.6925386553640713 1105 86-87 0.6331433990533029 1105 88-89 0.5329715563423711 1105 90-91 0.6681335355749702 1105 92-93 0.7970602387261394 1105 94-95 0.7697301489542525 1105 96-97 1.0793367799824587 1105 98-99 0.912742519523011 1105 100-101 1.069709173843151 1106 1 -0.20653684370009273 1106 2 -0.16061296731141894 1106 3 -0.1651191703910193 1106 4 -0.021470002202875094 1106 5 -0.18252956158408296 1106 6 -0.1477031833984057 1106 7 -0.45325708802261744 1106 8 -0.3448539995608897 1106 9 -0.5706703269542928 1106 10-11 -0.8751847934959969 1106 12-13 -0.6109100455868912 1106 14-15 -0.6793789539767658 1106 16-17 -0.45834541951360563 1106 18-19 -0.5766903998632813 1106 20-21 -0.44846955514914555 1106 22-23 -0.4694911482878297 1106 24-25 -0.5282717576058786 1106 26-27 -0.6627554024841658 1106 28-29 -0.39006475888538006 1106 30-31 -0.529753390138751 1106 32-33 -0.6010539505136094 1106 34-35 -0.5115352977591598 1106 36-37 -0.7400737062018621 1106 38-39 -0.7128522971457514 1106 40-41 -0.5619370224713975 1106 42-43 -0.5711813808979684 1106 44-45 -0.3546206003360197 1106 46-47 -0.6550519013194247 1106 48-49 -0.275391632791802 1106 50-51 -0.4365232386656217 1106 52-53 -0.37660300159254234 1106 54-55 -0.7154455954805243 1106 56-57 -0.7378890287617068 1106 58-59 -0.5675184461483695 1106 60-61 -0.7164712945547294 1106 62-63 -0.6292696136368292 1106 64-65 -0.5449563756440767 1106 66-67 -0.5651095838118394 1106 68-69 -0.5782564047344252 1106 70-71 -0.35559939832882037 1106 72-73 -0.26883144619104016 1106 74-75 -0.24105017688136599 1106 76-77 -0.1424290470152414 1106 78-79 -0.052623181006179465 1106 80-81 -0.5509942332751798 1106 82-83 -0.6734032928099083 1106 84-85 -0.5694041269341454 1106 86-87 -0.4880268883739731 1106 88-89 -0.16868195615541026 1106 90-91 -0.06635456287530417 1106 92-93 -0.06509115624603723 1106 94-95 0.12500105214430945 1106 96-97 0.27292665008777206 1106 98-99 0.13858833936805937 1106 100-101 0.39028467707033343 1107 1 0.02021316080576696 1107 2 -0.15707563081481624 1107 3 0.06775785380686727 1107 4 -0.04559484580214246 1107 5 0.0662687969551854 1107 6 0.04498916698099009 1107 7 0.26293640020475806 1107 8 0.12019049461137854 1107 9 0.2138025776552439 1107 10-11 0.03784738402915622 1107 12-13 0.1646550128517319 1107 14-15 0.12680128228203102 1107 16-17 0.052042245626125805 1107 18-19 0.11616937875200506 1107 20-21 0.05878819801136359 1107 22-23 0.37176601484229366 1107 24-25 0.2708338056597057 1107 26-27 0.1575869858867307 1107 28-29 0.1277533049612174 1107 30-31 0.06521304358290081 1107 32-33 0.21671962390374233 1107 34-35 0.20648946644212884 1107 36-37 0.22917262010012251 1107 38-39 0.3202733556109294 1107 40-41 0.4050018297013196 1107 42-43 0.28309534335199515 1107 44-45 0.26472015928541026 1107 46-47 0.26536988503075776 1107 48-49 -0.016279125760284785 1107 50-51 0.3151285143981255 1107 52-53 0.24655967509642096 1107 54-55 0.3664268118718965 1107 56-57 0.30331302059784804 1107 58-59 0.17364271846915358 1107 60-61 0.3411733458919457 1107 62-63 0.5017811621772381 1107 64-65 0.6554082669274734 1107 66-67 0.7428141827448229 1107 68-69 0.37755107840654745 1107 70-71 0.6605956076615982 1107 72-73 0.7915622425707802 1107 74-75 0.5686550640492882 1107 76-77 0.6253114686257817 1107 78-79 0.9073069260055995 1107 80-81 0.9589238739225294 1107 82-83 0.7439805959456507 1107 84-85 0.7427081913255869 1107 86-87 0.8952908088379914 1107 88-89 0.8139771267552405 1107 90-91 0.9614299868660581 1107 92-93 0.977972524348317 1107 94-95 1.0752008751351951 1107 96-97 1.0584098157241009 1107 98-99 1.3522476376113453 1107 100-101 1.6451944033702262 1108 1 -0.19784062502108313 1108 2 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0.6455025448185481 2312 8 0.6467605090531237 2312 9 0.7197685624283139 2312 10-11 0.6733824999136786 2312 12-13 0.5818007343082385 2312 14-15 0.5506666234858812 2312 16-17 1.0027264760771857 2312 18-19 0.811578164383171 2312 20-21 0.9977928390006952 2312 22-23 0.7225848480050345 2312 24-25 1.1486678236908574 2312 26-27 0.5970297867448195 2312 28-29 0.8670797305581743 2312 30-31 0.6955217885528313 2312 32-33 1.3099144753500127 2312 34-35 0.5934547361382911 2312 36-37 -0.01835966351901419 2312 38-39 1.0646719198486707 2312 40-41 1.2364049227594762 2312 42-43 1.0600474331993084 2312 44-45 1.2006436600776098 2312 46-47 1.449782610595463 2312 48-49 1.6008287386835676 2312 50-51 1.8581242660000399 2312 52-53 1.8680806857384482 2312 54-55 1.464486990500916 2312 56-57 1.7505515618409575 2312 58-59 1.3476903341784094 2312 60-61 1.311560749321508 2312 62-63 1.711158484424537 2312 64-65 1.838040310785523 2312 66-67 1.4743168257251469 2312 68-69 1.3122021629398546 2312 70-71 1.3862023714478582 2312 72-73 1.190161533102323 2312 74-75 1.3721666525015017 2312 76-77 1.6139473542695661 2312 78-79 1.5198754542090747 2312 80-81 1.7471699725470557 2312 82-83 2.165606063900391 2312 84-85 1.7395990088968496 2312 86-87 0.9825477177428041 2312 88-89 0.9694152165184242 2312 90-91 1.1836962198128766 2312 92-93 1.5506965467260159 2312 94-95 1.6110738999105045 2312 96-97 0.9350345125174719 2312 98-99 1.054933382342913 2312 100-101 1.3425521240976401 2313 1 0.13724007392515958 2313 2 0.0996225448730641 2313 3 -0.05650007863939521 2313 4 0.14294148991945832 2313 5 0.3363332198584317 2313 6 -0.09927599046377367 2313 7 -0.1760123625142498 2313 8 0.012513933710657454 2313 9 0.2466823416386248 2313 10-11 0.1313599374640475 2313 12-13 0.08490307113338247 2313 14-15 0.2555417242110991 2313 16-17 0.6313727290989419 2313 18-19 0.13385858340009094 2313 20-21 0.20162039742132265 2313 22-23 0.13729073035797512 2313 24-25 0.21264848444831586 2313 26-27 0.1894552500808402 2313 28-29 0.054952413878069706 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94-95 1.493185100555145 2313 96-97 2.0747202498260933 2313 98-99 2.5871654532534656 2313 100-101 2.3359042594723363 2314 1 0.3736719306184533 2314 2 0.42242574022528245 2314 3 0.5344464234182205 2314 4 0.4688674158453807 2314 5 0.5586764781494011 2314 6 0.5477586258518912 2314 7 0.6345661898943717 2314 8 0.5672464439987266 2314 9 0.5040292310116641 2314 10-11 0.63781115992834 2314 12-13 0.6446706818813865 2314 14-15 0.7968755416887134 2314 16-17 0.850956365022455 2314 18-19 0.7664657003209285 2314 20-21 0.7196063572373532 2314 22-23 0.8450128315441319 2314 24-25 1.230464993768095 2314 26-27 1.2151875182967657 2314 28-29 1.1569495090124207 2314 30-31 1.1398957667928613 2314 32-33 1.292537509342914 2314 34-35 1.2130076985375169 2314 36-37 1.111143126942686 2314 38-39 1.4383260153444724 2314 40-41 1.4611639283403974 2314 42-43 1.3672237114562549 2314 44-45 1.3156445056720614 2314 46-47 1.262229760942155 2314 48-49 1.448720389847665 2314 50-51 1.7331735923431282 2314 52-53 1.7138442575294164 2314 54-55 1.718996827583048 2314 56-57 1.5202680058344242 2314 58-59 1.6918895562315157 2314 60-61 1.2822186218058675 2314 62-63 1.2594419276851525 2314 64-65 1.2891931378896402 2314 66-67 1.3943235904807736 2314 68-69 1.3423757916675143 2314 70-71 0.918532266030418 2314 72-73 0.9869933725521172 2314 74-75 1.4518639296873594 2314 76-77 1.418248611386641 2314 78-79 1.637525829089281 2314 80-81 1.8081937055646975 2314 82-83 1.4384934870922237 2314 84-85 2.0749758621580234 2314 86-87 2.275757594286013 2314 88-89 2.291473957146419 2314 90-91 2.3743636757010336 2314 92-93 2.2637722556160327 2314 94-95 2.4586414065343263 2314 96-97 3.1942796785358496 2314 98-99 2.981803555407911 2314 100-101 2.896543329915332 2315 1 0.5006534213268985 2315 2 0.5466022336095442 2315 3 0.506728620912174 2315 4 0.3904751222064533 2315 5 0.3807805952211325 2315 6 0.42588887368816586 2315 7 0.6494954190737232 2315 8 0.3838143821411535 2315 9 0.6215438557509998 2315 10-11 0.4995081833094659 2315 12-13 0.7787569355491044 2315 14-15 0.6652621146094191 2315 16-17 0.7597293632324238 2315 18-19 0.94968554198622 2315 20-21 0.7239891655563753 2315 22-23 0.7371588390364252 2315 24-25 0.8436429450128031 2315 26-27 1.0340345167650966 2315 28-29 1.0206211028783372 2315 30-31 0.8634835798176468 2315 32-33 0.3588886138106062 2315 34-35 0.8384482861106406 2315 36-37 0.9350926369908521 2315 38-39 0.5654551374910355 2315 40-41 0.6225158632349306 2315 42-43 1.016432960565858 2315 44-45 0.7013500916765949 2315 46-47 0.8641508150306265 2315 48-49 0.3017163853449105 2315 50-51 0.5346093080787924 2315 52-53 0.573252993619775 2315 54-55 -0.05524702264485626 2315 56-57 -0.09919350849505548 2315 58-59 0.38834086553783465 2315 60-61 0.4239448023960293 2315 62-63 0.20086915461313026 2315 64-65 0.012651773406069822 2315 66-67 0.6100131215664177 2315 68-69 0.8021739056758932 2315 70-71 0.6136733463234663 2315 72-73 0.6741900360816153 2315 74-75 0.5374255750397374 2315 76-77 0.3029945930434401 2315 78-79 0.32898535039434407 2315 80-81 0.06804349057641801 2315 82-83 0.5064593104143036 2315 84-85 -0.05057544899368693 2315 86-87 0.5374804531688113 2315 88-89 0.6684139265128941 2315 90-91 0.5167172899031769 2315 92-93 0.43823570760813624 2315 94-95 0.8957965487218544 2315 96-97 0.8444116241336665 2315 98-99 1.0078328233405145 2315 100-101 1.032290437261839 2316 1 0.32506968852962004 2316 2 0.3818233562320934 2316 3 0.4452240592916361 2316 4 0.5429414899194569 2316 5 0.24911212452374087 2316 6 0.17902015558085083 2316 7 0.46724321557906023 2316 8 0.31078979577962684 2316 9 0.5842584065473488 2316 10-11 0.1436824526770195 2316 12-13 0.5513330914173551 2316 14-15 0.5234169777608031 2316 16-17 0.5655512280847432 2316 18-19 0.7998575692013006 2316 20-21 0.6276345962042882 2316 22-23 0.6622400204188281 2316 24-25 1.1176687684239752 2316 26-27 0.8544400370990957 2316 28-29 1.0304595132695553 2316 30-31 0.8654261343251477 2316 32-33 0.8721192462522396 2316 34-35 0.8186554919247797 2316 36-37 0.7378991933539183 2316 38-39 1.1600604413413507 2316 40-41 0.8720036473697661 2316 42-43 0.8268164459532059 2316 44-45 0.46416515275591763 2316 46-47 0.7318444906143569 2316 48-49 0.6365219366051491 2316 50-51 0.8135446722352739 2316 52-53 0.763333401846154 2316 54-55 0.4699192059000623 2316 56-57 1.0389018216425612 2316 58-59 1.1053367678109112 2316 60-61 1.4756786047773431 2316 62-63 1.3120973129554798 2316 64-65 1.4368534898124423 2316 66-67 1.3281784223241075 2316 68-69 1.4320061308189267 2316 70-71 1.4707264621422382 2316 72-73 1.3471643673016551 2316 74-75 1.1349205495255852 2316 76-77 1.2330722952237423 2316 78-79 1.5803383734343939 2316 80-81 1.6401789475116288 2316 82-83 1.2414504191200422 2316 84-85 1.724010912534073 2316 86-87 1.6118580625703913 2316 88-89 1.768334678020551 2316 90-91 1.732379498036778 2316 92-93 2.203418559007549 2316 94-95 3.0317753642468297 2316 96-97 2.7254808989133146 2316 98-99 3.2049392869248656 2316 100-101 3.084256186450027 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4907.0 3 1174.0 4 914.0 5 1005.0 6 1182.0 7 1340.0 8 1599.0 9 2069.0 10 2446.0 11 3027.0 12 3881.0 13 5051.0 14 5358.0 15 6451.0 16 7843.0 17 10292.0 18 9336.0 19 7092.0 20 7251.0 21 7680.0 22 8450.0 23 9532.0 24 10661.0 25 12080.0 26 13706.0 27 16008.0 28 19095.0 29 22347.0 30 27306.0 31 33656.0 32 41909.0 33 54472.0 34 72383.0 35 100761.0 36 145232.0 37 197667.0 38 153999.0 39 18441.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.803443641668295 19.160473548589653 14.699609843133201 23.336472966608852 2 14.798803557178369 19.897559846062283 40.520314841287615 24.783321755471736 3 19.27320530581941 23.82385245964378 30.19703556858453 26.705906665952284 4 12.180344652715982 15.960042483566323 38.00499468955421 33.854618174163484 5 13.291092004064659 37.147236739622585 35.00525305567091 14.556418200641849 6 31.77611776022051 36.09077031301593 17.598951030545493 14.534160896218062 7 28.11326966771755 32.48458606821754 21.08784744753712 18.314296816527794 8 25.96937540252082 34.584971726650444 20.137381014127584 19.308271856701154 9 25.506631157588917 16.6164110504301 20.864964387253096 37.01199340472789 10-11 24.44304188753748 26.407360584620598 27.98317952526373 21.166418002578194 12-13 25.536754037737868 23.78571182476275 28.27966909498781 22.39786504251157 14-15 22.191883458319257 25.13575892468406 26.85782799116981 25.81452962582687 16-17 21.62308948582579 28.670261558787203 27.30133786833967 22.405311087047338 18-19 21.545292520424397 27.363739668185787 29.55261300702145 21.538354804368367 20-21 22.179041010296828 26.385182364204045 29.390170503856645 22.04560612164248 22-23 21.832864882967147 26.283677594517336 29.529643937853578 22.35381358466194 24-25 21.999765588933883 26.322353487103804 29.480540293587897 22.197340630374413 26-27 21.785390830240654 26.404765584146233 29.69533809247457 22.11450549313854 28-29 21.397704832216757 27.018009262781543 29.61262165236194 21.97166425263976 30-31 21.613170110578743 26.45271872306889 29.47847736387244 22.455633802479923 32-33 21.467340463354052 26.557134032171703 29.535243933891874 22.44028157058237 34-35 22.076089297250736 26.481420333548684 29.23082812911253 22.211662240088053 36-37 21.867633838974616 26.25655613747281 29.527747597498248 22.348062426054327 38-39 21.590827543776893 26.669961315780156 29.59151454031034 22.147696600132615 40-41 21.770224369707698 26.696742094436203 29.41343347844807 22.11960005740803 42-43 21.515412474464043 26.684942517187434 29.14558006688387 22.654064941464654 44-45 21.699170450108333 26.64086844341681 29.016924042782875 22.643037063691978 46-47 21.94642360657495 26.96779281510311 28.756384323217475 22.32939925510447 48-49 21.95490211467524 26.96909630032332 28.54170295771224 22.534298627289207 50-51 21.588768587574084 27.106751251933414 28.92731894822279 22.377161212269712 52-53 21.948033422813136 27.390250020104233 28.138319056134854 22.52339750094777 54-55 21.75374536092095 27.00665443138231 28.428467853664724 22.811132354032022 56-57 21.917949777823704 27.243722227963215 28.311307070219954 22.527020923993128 58-59 22.31783429253658 27.042548062205352 28.16053962835976 22.479078016898306 60-61 21.924011301732044 26.9638720533728 28.927564736950607 22.184551907944552 62-63 21.805168023672703 27.072258478676726 28.844410619614646 22.27816287803592 64-65 21.641465149393905 27.257960444467617 28.607759863891243 22.49281454224724 66-67 21.60244596243523 27.184306732567087 28.27020002114196 22.94304728385572 68-69 21.445723541021497 27.085707124194826 28.62784603771852 22.840723297065157 70-71 21.766951280763436 27.090526416493265 28.397933558154552 22.744588744588746 72-73 21.75921917506136 26.709214558956273 28.690360759375594 22.84120550660677 74-75 21.528663090706697 27.364560874954137 28.348420261562033 22.75835577277713 76-77 21.64384295680073 27.060867851174386 28.278578916068742 23.01671027595614 78-79 21.661685720559596 26.989392426633703 28.3285309900641 23.020390862742605 80-81 21.79570405316905 27.024300261817295 28.304588284764225 22.87540740024943 82-83 21.72097152714063 26.927282798895185 28.592161999003103 22.759583674961085 84-85 21.592101725822083 26.878633731359564 28.385941615378655 23.143322927439698 86-87 21.55022709376432 27.025353940007445 28.47436237218501 22.950056594043225 88-89 21.601924214903192 26.742970640701756 28.197086601630016 23.458018542765036 90-91 21.44974837468562 26.50416632579199 28.60208849728568 23.44399680223671 92-93 21.770103868532374 26.503673165964344 28.603814237351056 23.122408728152234 94-95 21.42543818736974 26.488612462072236 28.589366975255047 23.496582375302978 96-97 21.029594687691365 26.554510486834047 28.94150910900184 23.47438571647275 98-99 21.26106686888646 26.769467043994766 28.332811038521434 23.636655048597337 100-101 21.27921226262122 26.31535898473486 28.762688661782054 23.642740090861867 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1606.0 1 936.5 2 267.0 3 252.5 4 196.0 5 179.0 6 295.0 7 592.0 8 1006.0 9 1174.5 10 1083.5 11 1060.5 12 1143.5 13 1188.5 14 1223.0 15 1313.0 16 1393.5 17 1468.5 18 1525.5 19 1616.0 20 1767.0 21 2043.0 22 2446.0 23 3024.0 24 3714.0 25 4712.5 26 6118.0 27 7633.0 28 8884.5 29 10298.0 30 12334.5 31 14250.0 32 16026.0 33 18008.5 34 20356.5 35 22433.5 36 24728.5 37 27240.5 38 28792.5 39 29672.0 40 30741.5 41 32716.0 42 34527.0 43 36628.5 44 38721.5 45 39820.0 46 40425.5 47 43022.0 48 48520.5 49 47839.0 50 41960.5 51 38288.0 52 35860.0 53 34620.5 54 33147.5 55 30096.0 56 26824.5 57 24060.0 58 21664.0 59 18959.0 60 15788.0 61 12657.0 62 9684.0 63 7313.0 64 5538.0 65 4169.0 66 3076.0 67 2238.0 68 1616.0 69 1163.5 70 842.5 71 561.5 72 364.5 73 277.5 74 211.0 75 163.5 76 104.5 77 56.0 78 36.5 79 23.0 80 18.0 81 17.0 82 12.0 83 5.0 84 5.0 85 5.0 86 4.5 87 4.0 88 2.0 89 3.0 90 3.0 91 2.0 92 1.5 93 0.5 94 0.5 95 1.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27691629224262104 2 0.2396886624685355 3 0.2307158491383713 4 0.23854319693702516 5 0.23892501878086192 6 0.26479344870080346 7 0.26488890416176264 8 0.2512387732445979 9 0.2499023967911692 10-11 0.25696610090214955 12-13 0.25935248742612943 14-15 0.2441750691336176 16-17 0.25777747232030274 18-19 0.24785010438054655 20-21 0.2415023162267602 22-23 0.2383045582846272 24-25 0.23257723062707555 26-27 0.24174095487915817 28-29 0.2552956303353637 30-31 0.25749110593742514 32-33 0.2562501849449556 34-35 0.2657480033103954 36-37 0.23868638012846397 38-39 0.2367295431788005 40-41 0.2347249784986574 42-43 0.23997502885141309 44-45 0.2582070218946191 46-47 0.26388662182169115 48-49 0.2687071226001304 50-51 0.26966167720972234 52-53 0.2914255223084185 54-55 0.2688503057915692 56-57 0.23797046417127002 58-59 0.21926119382326806 60-61 0.21787708963935973 62-63 0.2247498828284217 64-65 0.21654071318593102 66-67 0.21902255517087005 68-69 0.22336577864451337 70-71 0.2227930458787582 72-73 0.22484533828938089 74-75 0.22799536850103427 76-77 0.24183641034011735 78-79 0.23477270622913698 80-81 0.2304772104859733 82-83 0.22603853155137077 84-85 0.22603853155137077 86-87 0.23348405750618792 88-89 0.22942720041542217 90-91 0.23897274651134157 92-93 0.2284726458058302 94-95 0.22579989289897281 96-97 0.23758864232743324 98-99 0.2426000540277909 100-101 0.21821118375271692 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1047609.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.27828327390513 #Duplication Level Percentage of deduplicated Percentage of total 1 81.47984833702857 51.55904924185323 2 10.920384389789001 13.82046353754 3 3.26234624371107 6.193070092413284 4 1.4020129699155655 3.548678954560247 5 0.7565394210965956 2.393625789801329 6 0.4693121489283728 1.7818360262264834 7 0.3119121221581429 1.3816084535741509 8 0.220670870002018 1.117093905782943 9 0.17182828615872306 0.9785699069419179 >10 0.9324133357626728 10.63602760234626 >50 0.05256260181857231 2.281781895153292 >100 0.01776100400316103 1.9508937184912478 >500 0.0010536179620885449 0.4737460425785893 >1k 0.0013546516655424147 1.8835548327370693 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3835 0.36607169277850804 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2774 0.26479344870080346 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2553 0.24369779182882162 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2245 0.21429750985338994 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2025 0.19329730844236734 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1863 0.17783352376697795 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1668 0.15921970887993517 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1604 0.15311055937854676 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1209 0.11540565229966523 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.545546095919375E-5 2 0.0 0.0 0.0 0.0 9.545546095919375E-5 3 0.0 0.0 0.0 0.0 9.545546095919375E-5 4 0.0 0.0 0.0 0.0 9.545546095919375E-5 5 0.0 0.0 0.0 0.0 9.545546095919375E-5 6 0.0 0.0 0.0 0.0 9.545546095919375E-5 7 0.0 0.0 0.0 0.0 9.545546095919375E-5 8 0.0 0.0 0.0 0.0 9.545546095919375E-5 9 0.0 0.0 0.0 0.0 9.545546095919375E-5 10-11 0.0 0.0 0.0 4.7727730479596874E-5 9.545546095919375E-5 12-13 0.0 0.0 0.0 9.545546095919375E-5 9.545546095919375E-5 14-15 9.545546095919375E-5 0.0 0.0 9.545546095919375E-5 1.4318319143879062E-4 16-17 9.545546095919375E-5 0.0 0.0 1.4318319143879062E-4 1.909109219183875E-4 18-19 9.545546095919375E-5 0.0 0.0 1.909109219183875E-4 1.909109219183875E-4 20-21 9.545546095919375E-5 0.0 0.0 1.909109219183875E-4 1.909109219183875E-4 22-23 9.545546095919375E-5 0.0 0.0 2.8636638287758123E-4 1.909109219183875E-4 24-25 9.545546095919375E-5 0.0 0.0 4.772773047959687E-4 1.909109219183875E-4 26-27 9.545546095919375E-5 0.0 0.0 5.727327657551625E-4 1.909109219183875E-4 28-29 1.4318319143879062E-4 0.0 0.0 0.0016704705667858906 1.909109219183875E-4 30-31 1.909109219183875E-4 0.0 0.0 0.0037704907078881526 1.909109219183875E-4 32-33 1.909109219183875E-4 0.0 0.0 0.0061091495013884 1.909109219183875E-4 34-35 1.909109219183875E-4 0.0 0.0 0.00902054106064381 1.909109219183875E-4 36-37 3.81821843836775E-4 0.0 0.0 0.012647848577093172 1.909109219183875E-4 38-39 3.81821843836775E-4 0.0 0.0 0.017659260277450843 1.909109219183875E-4 40-41 3.81821843836775E-4 0.0 0.0 0.021907028290134963 2.3863865239798438E-4 42-43 4.2954957431637185E-4 0.0 0.0 0.033457139066197406 2.8636638287758123E-4 44-45 4.772773047959687E-4 0.0 0.0 0.047345908635760096 2.8636638287758123E-4 46-47 5.250050352755656E-4 0.0 0.0 0.0610437672834044 2.8636638287758123E-4 48-49 5.727327657551625E-4 0.0 0.0 0.08170987458106985 2.8636638287758123E-4 50-51 5.727327657551625E-4 0.0 0.0 0.106910116274297 2.8636638287758123E-4 52-53 6.204604962347593E-4 0.0 0.0 0.1554969459025266 3.340941133571781E-4 54-55 6.681882267143562E-4 0.0 0.0 0.1938700412081225 3.81821843836775E-4 56-57 6.681882267143562E-4 0.0 0.0 0.2653661814665586 3.81821843836775E-4 58-59 6.681882267143562E-4 0.0 0.0 0.35471249292436396 3.81821843836775E-4 60-61 6.681882267143562E-4 0.0 0.0 0.44477472033936327 3.81821843836775E-4 62-63 6.681882267143562E-4 0.0 0.0 0.57182593887605 3.81821843836775E-4 64-65 6.681882267143562E-4 0.0 0.0 0.6482380353738847 3.81821843836775E-4 66-67 6.681882267143562E-4 0.0 0.0 0.8192942214127599 3.81821843836775E-4 68-69 6.681882267143562E-4 0.0 0.0 1.0101096878701883 3.81821843836775E-4 70-71 6.681882267143562E-4 0.0 0.0 1.1615975044124287 3.81821843836775E-4 72-73 6.681882267143562E-4 0.0 0.0 1.4231454674406194 3.81821843836775E-4 74-75 6.681882267143562E-4 0.0 0.0 1.689848025360607 3.81821843836775E-4 76-77 6.681882267143562E-4 0.0 0.0 1.870402029764922 3.81821843836775E-4 78-79 6.681882267143562E-4 0.0 0.0 2.0832199799734443 3.81821843836775E-4 80-81 6.681882267143562E-4 0.0 0.0 2.4444234442430335 3.81821843836775E-4 82-83 6.681882267143562E-4 0.0 0.0 2.747064983214157 4.772773047959687E-4 84-85 6.681882267143562E-4 0.0 0.0 3.171364507177773 4.772773047959687E-4 86-87 7.159159571939531E-4 0.0 0.0 3.6778511830272556 4.772773047959687E-4 88-89 7.6364368767355E-4 0.0 0.0 4.366085056543042 4.772773047959687E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTATAC 215 1.8189894E-12 28.72352 3 GTACATG 3510 0.0 27.074446 1 GTATAGA 345 0.0 26.16804 1 TACATGG 3470 0.0 25.73596 2 GTATCAA 6120 0.0 25.155346 1 ACATGGG 3615 0.0 23.25936 3 GGTATCA 4555 0.0 22.427807 1 GAGTACT 3225 0.0 21.870401 12-13 GTGTAGG 350 0.0 21.721441 1 TCAACGC 7040 0.0 21.662407 4 ATCAACG 7045 0.0 21.644962 3 CAACGCA 7045 0.0 21.579597 5 AACGCAG 7110 0.0 21.382317 6 AGAGTAC 5130 0.0 21.156782 10-11 TATCAAC 7260 0.0 21.133814 2 CATGGGG 2035 0.0 20.54436 4 AGTACTT 3480 0.0 20.540796 12-13 GTACTTT 3475 0.0 20.365818 14-15 ACCGCGT 60 0.004765592 19.791082 10-11 GTGTAGC 570 0.0 19.172983 1 >>END_MODULE