FastQCFastQC Report
Thu 26 May 2016
SRR937352_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937352_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047609
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT46250.4414815069362711No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45820.43737692211502577No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36720.3505124526421594No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26490.25286151608090424No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15000.14318319143879063No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT13070.12476028747366623No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT11930.11387836492431813No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC11090.10586010620374586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG2409.094947E-1225.741791
GTACATG36000.025.2137551
TACATGG36650.023.9759752
ACATGGG37650.022.7083763
GAGTACT39700.022.55597312-13
CATGGGG19600.021.5681294
GTACTTT42250.021.07814614-15
AGTACTT41100.020.97855412-13
AGAGTAC61750.019.96370510-11
GTATCAA99450.018.6843761
TATGACG1300.00756420218.2685994
ACTTTTT50600.017.92836416-17
GTTACGT1203.094996E-617.81103118-19
TACTTTT49950.017.1632514-15
TCAACGC109900.016.7692094
CAACGCA110000.016.7539655
ATCAACG110700.016.6480223
AACGCAG111800.016.525926
TATCAAC112150.016.3904252
TAGACAG6100.016.3518925
Produced by FastQC (version 0.11.5)