FastQCFastQC Report
Thu 26 May 2016
SRR937351_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937351_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1821349
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62390.3425482979923123No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43350.23801039778757394No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32080.17613318479873982No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31190.17124669681648053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38950.046.9947171
GTATCAA67750.038.9474871
ATCAACG85450.030.6961273
TCAACGC86000.030.5542244
TATCAAC87550.030.0157662
CAACGCA87900.029.8946045
AACGCAG90450.029.093936
CGCAGAG105300.024.6758488
ACGCAGA106100.024.6241027
GTACATG75000.024.4693131
TACATGG73950.023.7762852
CCGTCCG1201.7108282E-423.7510649
ACATGGG75000.022.4917723
GCAGAGT112900.022.0891239
GAGTACT60100.020.78540812-13
AGAGTAC99950.019.8409710-11
GTACTTT63150.019.66788714-15
CATGGGA46150.019.1507534
AGTACTT64250.019.07321212-13
CAGAGTA109500.018.97811710-11
Produced by FastQC (version 0.11.5)