##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937351_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1821349 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.713376184355663 33.0 31.0 34.0 30.0 34.0 2 31.844654154695228 33.0 31.0 34.0 30.0 34.0 3 31.881020057111513 33.0 31.0 34.0 30.0 34.0 4 35.50417904531202 37.0 35.0 37.0 33.0 37.0 5 35.509088044081615 37.0 35.0 37.0 33.0 37.0 6 35.54002665057603 37.0 35.0 37.0 33.0 37.0 7 35.47589067224349 37.0 35.0 37.0 33.0 37.0 8 35.45518459120136 37.0 35.0 37.0 33.0 37.0 9 37.06325037101621 39.0 37.0 39.0 33.0 39.0 10-11 36.94610368468646 39.0 37.0 39.0 33.0 39.0 12-13 36.81288100193868 39.0 37.0 39.0 33.0 39.0 14-15 37.93166850504763 40.0 38.0 41.0 33.0 41.0 16-17 37.85099698080928 40.0 38.0 41.0 33.0 41.0 18-19 37.761676098320535 40.0 37.5 41.0 33.0 41.0 20-21 37.63049723034959 40.0 37.0 41.0 32.0 41.0 22-23 37.70361336569762 40.0 37.0 41.0 32.0 41.0 24-25 37.6751125127584 40.0 37.0 41.0 32.0 41.0 26-27 37.59846410545151 40.0 37.0 41.0 32.0 41.0 28-29 37.46056796363574 40.0 37.0 41.0 32.0 41.0 30-31 37.2200245532295 39.5 37.0 41.0 31.5 41.0 32-33 36.989554720155226 39.0 36.0 41.0 31.0 41.0 34-35 37.01405139816696 39.0 36.0 41.0 31.0 41.0 36-37 36.883988461299836 39.0 36.0 41.0 30.5 41.0 38-39 36.74494015150309 39.0 36.0 41.0 30.0 41.0 40-41 36.526032078421 39.0 35.5 41.0 30.0 41.0 42-43 36.27292078563746 39.0 35.0 40.5 30.0 41.0 44-45 36.03944109558355 39.0 35.0 40.0 29.5 41.0 46-47 35.6687482739442 39.0 35.0 40.0 27.5 41.0 48-49 35.35029338144419 39.0 35.0 40.0 26.5 41.0 50-51 34.402693278443614 37.5 34.0 39.5 25.0 40.5 52-53 34.52539024646018 38.0 34.0 39.5 26.0 40.5 54-55 34.83234651898126 38.0 34.0 40.0 26.0 41.0 56-57 34.567846140415696 38.0 34.0 40.0 24.5 41.0 58-59 34.29230971109875 38.0 34.0 40.0 23.5 41.0 60-61 33.955598295549066 38.0 33.0 40.0 22.5 41.0 62-63 33.56232331090857 37.0 33.0 40.0 22.0 41.0 64-65 33.12641371862284 37.0 32.0 39.5 20.5 41.0 66-67 32.68353593957006 36.0 31.5 39.0 18.5 40.5 68-69 32.162733501377275 35.5 31.0 39.0 17.0 40.0 70-71 31.678832283104448 35.0 31.0 38.0 12.5 40.0 72-73 31.164415221904203 35.0 30.0 37.5 8.0 39.5 74-75 30.588250796524996 34.5 29.5 37.0 7.0 39.0 76-77 30.0227732301717 34.0 29.0 36.5 2.0 39.0 78-79 29.439254365857394 34.0 29.0 36.0 2.0 38.5 80-81 28.825921061806387 34.0 28.0 35.5 2.0 37.0 82-83 28.217622762029684 33.5 26.5 35.0 2.0 37.0 84-85 27.586853206057707 33.0 26.0 35.0 2.0 36.0 86-87 26.944196032720804 32.5 25.5 35.0 2.0 36.0 88-89 26.337626122176474 32.0 24.5 35.0 2.0 35.5 90-91 25.71193329779191 32.0 22.0 34.0 2.0 35.0 92-93 24.975931575991204 31.0 19.0 34.0 2.0 35.0 94-95 24.126664631545083 31.0 12.0 34.0 2.0 35.0 96-97 23.199932851968512 31.0 2.0 34.0 2.0 35.0 98-99 21.939446531115124 30.0 2.0 34.0 2.0 35.0 100-101 18.84872641102831 24.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17242260663379128 1101 2 0.24367626998911263 1101 3 0.1920390147080724 1101 4 0.09498225313431163 1101 5 0.05318526245358868 1101 6 0.034002929643676794 1101 7 0.18375036358141017 1101 8 0.11851188842377525 1101 9 0.19817255321721206 1101 10-11 0.292571580388298 1101 12-13 0.24028235178123225 1101 14-15 0.3552105122108884 1101 16-17 0.4362887943515119 1101 18-19 0.3628866849982302 1101 20-21 0.44056826864915877 1101 22-23 0.3904699995565153 1101 24-25 0.2659070029603683 1101 26-27 0.33644801357566934 1101 28-29 0.2688954253504292 1101 30-31 0.29444411847851626 1101 32-33 0.24808817562606578 1101 34-35 0.4569985566769148 1101 36-37 0.5021334216732427 1101 38-39 0.50352947552296 1101 40-41 0.6230252865066959 1101 42-43 0.6717689995693732 1101 44-45 0.579091863857002 1101 46-47 0.649341874948064 1101 48-49 0.7207176371349746 1101 50-51 0.7323684255099963 1101 52-53 0.7185835933313811 1101 54-55 0.706080161224584 1101 56-57 0.7321688028885518 1101 58-59 0.7344019111355138 1101 60-61 0.8655019738560341 1101 62-63 0.8868052150552828 1101 64-65 0.8720155226862474 1101 66-67 1.0285956420694333 1101 68-69 1.1332162949237414 1101 70-71 1.0631382794251856 1101 72-73 1.0349167946460476 1101 74-75 1.0123428427313037 1101 76-77 1.081080281042773 1101 78-79 1.2056910662521965 1101 80-81 1.3054613333279086 1101 82-83 1.1265306965595805 1101 84-85 1.4003151115956136 1101 86-87 1.4702963804551246 1101 88-89 1.4787747283657176 1101 90-91 1.6400342360681108 1101 92-93 1.9259277968876383 1101 94-95 2.013107095394602 1101 96-97 1.7840635837693881 1101 98-99 2.1621433784415736 1101 100-101 2.1108802218132148 1102 1 0.01608036602472751 1102 2 0.0661289019485416 1102 3 -0.0471279554131776 1102 4 0.0013823716682992426 1102 5 -0.051007996941379474 1102 6 0.060456800713467373 1102 7 -0.16859774756690626 1102 8 -0.09520746502827393 1102 9 0.0857067086610428 1102 10-11 0.03346958589260396 1102 12-13 0.040433387026872936 1102 14-15 0.2678025231752841 1102 16-17 0.20580261679488387 1102 18-19 0.08363555514645782 1102 20-21 0.11596130830218954 1102 22-23 0.17713547829432486 1102 24-25 0.13519557198804222 1102 26-27 0.2359539377360278 1102 28-29 0.17681254296703486 1102 30-31 0.2657480192564847 1102 32-33 0.27275305870668376 1102 34-35 0.3168180731430752 1102 36-37 0.38763296268262337 1102 38-39 0.39587504153620756 1102 40-41 0.3404657051037461 1102 42-43 0.44959946907808757 1102 44-45 0.36397731634885844 1102 46-47 0.3996456780430364 1102 48-49 0.4212549384493869 1102 50-51 0.33467882265561144 1102 52-53 0.40619253452233295 1102 54-55 0.5619324633321199 1102 56-57 0.5178741055221394 1102 58-59 0.5000535209782555 1102 60-61 0.5591900388752791 1102 62-63 0.634106610129848 1102 64-65 0.4576881448295822 1102 66-67 0.6221728204526329 1102 68-69 0.7044315341888421 1102 70-71 0.7610993036090399 1102 72-73 0.9088061732776076 1102 74-75 0.8773305218280107 1102 76-77 0.8824225560051886 1102 78-79 0.9523951611415242 1102 80-81 1.122979106705138 1102 82-83 1.166002395822595 1102 84-85 1.2169417889725587 1102 86-87 1.149178898872254 1102 88-89 1.3366459618570659 1102 90-91 1.2485003799465062 1102 92-93 1.308848895011856 1102 94-95 1.2482501681313956 1102 96-97 1.2794724080394637 1102 98-99 1.553845864697994 1102 100-101 1.5576715891452118 1103 1 0.12657183984068965 1103 2 0.1813485142971416 1103 3 0.13146596043595693 1103 4 0.019775217398233735 1103 5 0.182510182761213 1103 6 0.1478540478338175 1103 7 0.1570924524053865 1103 8 0.14444584252809278 1103 9 0.11378793423711642 1103 10-11 0.0265348654877684 1103 12-13 -0.035606878371488904 1103 14-15 0.022933095515050184 1103 16-17 0.0704122330669108 1103 18-19 0.06652286787024053 1103 20-21 -0.0019939212425725827 1103 22-23 -0.0899263696046475 1103 24-25 -0.08306169697259946 1103 26-27 -0.013856771590255335 1103 28-29 -0.16982187497026047 1103 30-31 0.031116418967435777 1103 32-33 -0.05514249993474607 1103 34-35 0.11662743694598987 1103 36-37 0.01667201741576463 1103 38-39 0.016797073655688166 1103 40-41 0.03521599278581533 1103 42-43 -0.27768022211207466 1103 44-45 -0.0883361123107207 1103 46-47 -0.12051186745323861 1103 48-49 -0.08875231514475956 1103 50-51 -0.06691999548558414 1103 52-53 -0.11218203961188067 1103 54-55 0.014702299633910343 1103 56-57 -0.03413541833689493 1103 58-59 -0.0955249669164644 1103 60-61 -0.13086758316293157 1103 62-63 -0.08773334414681955 1103 64-65 -0.21634888091548277 1103 66-67 0.004638296310730539 1103 68-69 0.10083158641960921 1103 70-71 0.07495042695286358 1103 72-73 0.20440473699257566 1103 74-75 0.22906003921629647 1103 76-77 0.20715949854185922 1103 78-79 0.2845195621160457 1103 80-81 0.23491449528463804 1103 82-83 0.46927755259900295 1103 84-85 0.6168497582322452 1103 86-87 0.8305045329577858 1103 88-89 0.894699484941814 1103 90-91 0.7433639209420058 1103 92-93 0.9080595314963951 1103 94-95 0.7383471925982086 1103 96-97 1.1127875207438649 1103 98-99 1.4881868472297342 1103 100-101 1.5888767988893697 1104 1 0.030638164357075226 1104 2 0.057159241272497496 1104 3 -0.10567050488842611 1104 4 0.1018053448854701 1104 5 0.13363425655180805 1104 6 0.10677990005390114 1104 7 0.14847424001975895 1104 8 0.0527984453141741 1104 9 0.15708847593030129 1104 10-11 0.15025549467904398 1104 12-13 0.1355152617597284 1104 14-15 0.36042648213143735 1104 16-17 0.26067894377803924 1104 18-19 0.2889850070883426 1104 20-21 0.1152852051730946 1104 22-23 0.13946068723150518 1104 24-25 0.14505102462610608 1104 26-27 0.17064655994369815 1104 28-29 0.29576973079955593 1104 30-31 0.17447711895213303 1104 32-33 0.2769675252833963 1104 34-35 0.41454794743206236 1104 36-37 0.43679174315840896 1104 38-39 0.4272592451014461 1104 40-41 0.4705471722374668 1104 42-43 0.3566312205858182 1104 44-45 0.33044616570115437 1104 46-47 0.5136961781811209 1104 48-49 0.4939908492350824 1104 50-51 0.374590837035349 1104 52-53 0.36324840102075484 1104 54-55 0.5502614897529128 1104 56-57 0.5467841978317196 1104 58-59 0.6209481995313268 1104 60-61 0.4880470968030153 1104 62-63 0.48482722370447817 1104 64-65 0.6388106325543319 1104 66-67 0.6531701922906024 1104 68-69 0.7623955408604459 1104 70-71 0.701783710601898 1104 72-73 0.8362534496319931 1104 74-75 0.8321227408070015 1104 76-77 0.8596164544785978 1104 78-79 0.8108079298121211 1104 80-81 0.8842238834694456 1104 82-83 1.0687656699936134 1104 84-85 0.8852846489670618 1104 86-87 0.9404196399401741 1104 88-89 1.044317721498178 1104 90-91 1.1028593969847726 1104 92-93 1.1194420710894128 1104 94-95 0.9244390790172083 1104 96-97 1.1177492369739817 1104 98-99 1.5310482871076978 1104 100-101 1.4643612208460723 1105 1 0.0016852038625323473 1105 2 -0.039093052748558677 1105 3 0.07451881221772538 1105 4 0.05856046072491239 1105 5 0.02701574707904797 1105 6 0.09228444670014113 1105 7 0.09561171569968963 1105 8 0.0077390422425978045 1105 9 0.013565549518361308 1105 10-11 0.10134110133549967 1105 12-13 0.19266053366158786 1105 14-15 0.15494083959856653 1105 16-17 0.1063102997687082 1105 18-19 0.32671906622924496 1105 20-21 0.3534201143091593 1105 22-23 0.3324102808620566 1105 24-25 0.2654026135375389 1105 26-27 0.30096937872638563 1105 28-29 0.2069083241679479 1105 30-31 0.3101124075503279 1105 32-33 0.32800690655314213 1105 34-35 0.3617595907100579 1105 36-37 0.45654792149181134 1105 38-39 0.35987711983754167 1105 40-41 0.26333325218668335 1105 42-43 0.3513946431962367 1105 44-45 0.3272690327790144 1105 46-47 0.2277921614019931 1105 48-49 0.21572092057396475 1105 50-51 0.20111282514093176 1105 52-53 0.0979233054652866 1105 54-55 0.34551407354504704 1105 56-57 0.37349591700214546 1105 58-59 0.39618933046645566 1105 60-61 0.45217930140899654 1105 62-63 0.35850083107846586 1105 64-65 0.4346910536883044 1105 66-67 0.42989640001506757 1105 68-69 0.29636775043393015 1105 70-71 0.3615131196068724 1105 72-73 0.4393067399136221 1105 74-75 0.38900411404595303 1105 76-77 0.38052136816821047 1105 78-79 0.3230938786744204 1105 80-81 0.3505034987638389 1105 82-83 0.642738362518898 1105 84-85 0.7420721915703083 1105 86-87 0.8394119955030881 1105 88-89 0.7151791695256655 1105 90-91 0.6939734288043944 1105 92-93 1.046645711166132 1105 94-95 1.1754032577921656 1105 96-97 1.0102113942506818 1105 98-99 1.0399984188845472 1105 100-101 0.8191427886456033 1106 1 -0.003821986176610892 1106 2 -0.6420918367779826 1106 3 -0.182467162026537 1106 4 0.03624546065536549 1106 5 0.016649433197585495 1106 6 -0.05936377542788307 1106 7 0.005985679727167792 1106 8 -0.07608056951065834 1106 9 -0.05410255420444088 1106 10-11 -0.3512079529380756 1106 12-13 -0.1517862839142694 1106 14-15 -0.14278728665738782 1106 16-17 -0.05867974040005208 1106 18-19 0.0964924059462362 1106 20-21 -0.05626268281848468 1106 22-23 0.05757951746533507 1106 24-25 -0.01328159063131551 1106 26-27 0.05510823583117741 1106 28-29 0.17296867100872504 1106 30-31 0.30382016245575727 1106 32-33 0.2712776880466805 1106 34-35 0.3557501081893619 1106 36-37 0.2805120221750528 1106 38-39 0.3230969381813438 1106 40-41 0.568690843437551 1106 42-43 0.3499960208081774 1106 44-45 0.3712729288629433 1106 46-47 0.440775703705107 1106 48-49 0.5995957305099253 1106 50-51 0.5548223589798269 1106 52-53 0.6395579440087005 1106 54-55 0.7114595400831618 1106 56-57 0.43088326768703666 1106 58-59 0.3025273849281689 1106 60-61 0.6723720082209965 1106 62-63 0.6805178048856746 1106 64-65 0.715867045846359 1106 66-67 0.9516571305344712 1106 68-69 0.8691305219683585 1106 70-71 0.8284233098209874 1106 72-73 0.8517137576963698 1106 74-75 0.7567746345560309 1106 76-77 0.835468230766466 1106 78-79 0.8548618420591403 1106 80-81 0.8224155667410606 1106 82-83 0.8892905808050173 1106 84-85 0.570948125235855 1106 86-87 0.8213332180330255 1106 88-89 1.159730984440447 1106 90-91 0.9768742726630855 1106 92-93 1.1359061689889387 1106 94-95 1.0951520365140723 1106 96-97 0.8725998174201592 1106 98-99 1.036298804612965 1106 100-101 0.7656793406040059 1107 1 0.1471565253828757 1107 2 0.20098383938483622 1107 3 0.11799540056344426 1107 4 0.20136484740133653 1107 5 0.030934542127880604 1107 6 0.14632985658269604 1107 7 0.16527185598716443 1107 8 0.07596487386685169 1107 9 0.1627517854308067 1107 10-11 0.10268171282360328 1107 12-13 0.09489151560799058 1107 14-15 0.13187374912391903 1107 16-17 0.2998809699700473 1107 18-19 0.33328144052115505 1107 20-21 0.25099866056864073 1107 22-23 0.30610967614433804 1107 24-25 0.14881585050291335 1107 26-27 0.26004349676394867 1107 28-29 0.3426321169856337 1107 30-31 0.26410654501975017 1107 32-33 0.1715202593623033 1107 34-35 0.3343871012997681 1107 36-37 0.19848062219060836 1107 38-39 0.32734029492431915 1107 40-41 0.2514488233932397 1107 42-43 0.356492209157949 1107 44-45 0.3715397115532255 1107 46-47 0.33948084278323876 1107 48-49 0.13624373432486436 1107 50-51 -0.03992329525746641 1107 52-53 0.11929807658040659 1107 54-55 0.3276511604806345 1107 56-57 0.2679184346589807 1107 58-59 0.3306529649716339 1107 60-61 0.34465533581481367 1107 62-63 0.4202860123069385 1107 64-65 0.25537774132555313 1107 66-67 0.6206174121423942 1107 68-69 0.5629744069450311 1107 70-71 0.5954147908787171 1107 72-73 0.4685339388022278 1107 74-75 0.26434730399238404 1107 76-77 0.5811267453419333 1107 78-79 0.7001931350320945 1107 80-81 0.6067056472593002 1107 82-83 0.6088197103513906 1107 84-85 0.6724983296200442 1107 86-87 0.6046065998647059 1107 88-89 0.7281066319753542 1107 90-91 0.5543165528055987 1107 92-93 0.6064154414281795 1107 94-95 0.7928756102562069 1107 96-97 0.698235246638891 1107 98-99 0.8138730636046425 1107 100-101 0.9126160247756765 1108 1 -0.052135522834447556 1108 2 0.032511693119811724 1108 3 -0.06626602232511303 1108 4 -0.05682702140354934 1108 5 -0.05377660875718249 1108 6 0.003333294451550728 1108 7 0.06548588649403086 1108 8 -0.09728689831433712 1108 9 -0.07010996878458542 1108 10-11 0.03653572891854395 1108 12-13 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2316 16-17 -0.3259557100835053 2316 18-19 -0.16195042326357623 2316 20-21 -0.28173652486724166 2316 22-23 -0.40579411881076766 2316 24-25 -0.3862858546949042 2316 26-27 -0.524914061308543 2316 28-29 -0.2595168032897348 2316 30-31 -0.42616013969664124 2316 32-33 -0.22767702916759447 2316 34-35 -0.1848408717660277 2316 36-37 -0.18782364606844482 2316 38-39 -0.35137205760690193 2316 40-41 -0.2756082187578883 2316 42-43 -0.26387690775915473 2316 44-45 -0.16049103314842483 2316 46-47 -0.22062976193274864 2316 48-49 -0.4292241296403603 2316 50-51 -0.3850441030830254 2316 52-53 -0.211382949646854 2316 54-55 0.06525325424897233 2316 56-57 -0.05316364647453753 2316 58-59 0.14736550802775383 2316 60-61 -0.07209531222051879 2316 62-63 -0.011593816621761732 2316 64-65 0.1470041721424522 2316 66-67 0.24058793092163455 2316 68-69 0.280977505481502 2316 70-71 0.3389381978649624 2316 72-73 0.30301543532514685 2316 74-75 0.39051238880103156 2316 76-77 0.14501525819483874 2316 78-79 0.4763790271649171 2316 80-81 0.4233204209608452 2316 82-83 0.5205089141077366 2316 84-85 0.7024834385952694 2316 86-87 1.0892267283185966 2316 88-89 1.0407604933634822 2316 90-91 0.8641601719764189 2316 92-93 0.8675323184243027 2316 94-95 0.7593708045163972 2316 96-97 0.9250676699651557 2316 98-99 1.3230182586169015 2316 100-101 2.112825100780068 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7396.0 3 1256.0 4 1195.0 5 1353.0 6 1539.0 7 1857.0 8 2409.0 9 3013.0 10 3987.0 11 5199.0 12 6527.0 13 8462.0 14 9615.0 15 11886.0 16 14636.0 17 19043.0 18 16769.0 19 12830.0 20 13531.0 21 14598.0 22 16310.0 23 18260.0 24 21120.0 25 24002.0 26 27712.0 27 32720.0 28 38630.0 29 46048.0 30 55631.0 31 68046.0 32 84743.0 33 108201.0 34 141465.0 35 189002.0 36 257015.0 37 311706.0 38 205391.0 39 18242.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.53643036418245 19.53219512087631 14.91722324932626 23.01415126561498 2 14.688996453523872 20.18776886952701 41.26240454495149 23.86083013199763 3 18.680235634452757 24.89606062758403 30.295457060692005 26.12824667727121 4 12.110989367969562 16.577928763744282 38.42788003589893 32.88320183238723 5 13.138378268255014 37.67034019860749 35.07153889144314 14.119742641694359 6 31.298771634543936 36.70422910408248 18.10707991585603 13.889919345517551 7 27.733029950771886 32.93924422956681 21.45132669326218 17.876399126399125 8 25.870909647320943 35.225634014297384 20.299080379296754 18.604375959084916 9 25.8419655606333 16.694703744601302 20.900210388177747 36.56312030658765 10-11 24.03346753782604 27.013143085367435 28.312725553142805 20.64066382366372 12-13 25.08901670021278 24.293928368838635 28.727382519561377 21.889672411387203 14-15 22.12966010552283 25.67473627548218 26.915119398434 25.28048422056099 16-17 21.38917630741797 29.10550155756382 27.445898048367035 22.059424086651184 18-19 21.328830743738983 27.569088638335355 29.924445974290336 21.177634643635333 20-21 22.000376983116933 26.751299835035493 29.953702620858174 21.2946205609894 22-23 21.722245106464005 26.793218816735198 29.83716233934368 21.64737373745712 24-25 21.48067983386633 26.83049445514837 30.029371121204168 21.659454589781138 26-27 21.38381323895087 27.15368692270377 29.68397412315084 21.77852571519452 28-29 21.27201618875654 27.184198294104 29.84170388148721 21.702081635652252 30-31 21.460789049587063 26.950418443727887 29.9110815679701 21.677710938714956 32-33 21.20541833037746 27.03393804885943 29.808330608645683 21.95231301211743 34-35 21.597293681615138 27.0301860070988 29.70691917125054 21.665601140035516 36-37 21.59205138301962 26.880791933827936 29.599390870934037 21.927765812218407 38-39 21.420293619318407 27.100356527524266 29.71774399904031 21.761605854117015 40-41 21.65471683578016 26.977362269715844 29.64648493385865 21.721435960645348 42-43 21.401886054691786 27.009832018352586 29.538265788367944 22.05001613858768 44-45 21.499949783380636 27.192946009567297 29.36863263584971 21.93847157120235 46-47 21.587600194727337 27.28753021287088 28.986550989749293 22.138318602652486 48-49 21.67761825291284 27.445364996609396 28.789883662559777 22.08713308791799 50-51 21.707774328857443 27.49386805085905 28.72386369360375 22.074493926679754 52-53 21.79539415509406 27.545239712941317 28.550903142471096 22.10846298949353 54-55 21.68684314745945 27.42436331040883 28.63456685677611 22.25422668535561 56-57 21.753563160706925 27.705159972860493 28.48324718796309 22.05802967846949 58-59 21.81156282513809 27.5021912003156 28.553692248185282 22.132553726361035 60-61 21.70910947680951 27.432561215697365 28.83371867685397 22.024610630639156 62-63 21.660934783087182 27.457189707921554 28.922671846455223 21.95920366253604 64-65 21.617981311558214 27.44242462256952 28.821086478197643 22.118507587674628 66-67 21.550761459724686 27.570798353139587 28.571872691582335 22.306567495553395 68-69 21.59422554616966 27.52315966245989 28.683151166731964 22.19946362463849 70-71 21.548929005242517 27.37308804978671 28.77204614954996 22.305936795420813 72-73 21.55317688393406 27.363173265944262 28.63106546360481 22.452584386516865 74-75 21.573891843154495 27.556946920344334 28.45781832399179 22.41134291250938 76-77 21.577169286769983 27.42009737433809 28.509415338453287 22.493318000438634 78-79 21.530220172575056 27.474144974164926 28.4830571602248 22.512577693035222 80-81 21.486697797636587 27.586403299871805 28.42493636366388 22.501962538827726 82-83 21.617487198774672 27.437061387278373 28.530767924948268 22.41468348899869 84-85 21.584266219622165 27.36011932434953 28.405142699003665 22.650471757024636 86-87 21.58459700339562 27.30193087666758 28.49954373901817 22.613928380918637 88-89 21.750396535524494 27.035427508628864 28.549127071579996 22.665048884266653 90-91 21.58511259974842 26.995903862936782 28.659285476101648 22.75969806121315 92-93 21.59586694294485 26.850646072184325 28.733793167205075 22.819693817665755 94-95 21.39868070481265 26.94042482009361 28.581482545310877 23.079411929782857 96-97 21.34503133156539 26.900378994451284 28.728599360436547 23.02599031354678 98-99 21.278452820365786 26.92339442195118 28.57906638482362 23.21908637285942 100-101 21.693143236486577 26.24873825570196 29.025002139015953 23.03311636879551 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2784.0 1 1558.5 2 315.0 3 289.0 4 239.5 5 219.0 6 352.0 7 726.5 8 1234.5 9 1519.0 10 1465.5 11 1450.0 12 1582.5 13 1671.0 14 1832.0 15 2051.0 16 2113.0 17 2230.5 18 2434.0 19 2571.5 20 2850.5 21 3411.0 22 4169.5 23 5037.5 24 6200.5 25 7796.0 26 10003.0 27 12961.0 28 15991.0 29 19212.5 30 23118.0 31 27252.5 32 31549.5 33 35756.0 34 40606.0 35 45098.5 36 49266.5 37 53689.0 38 56768.0 39 59140.0 40 61103.0 41 63213.5 42 65813.5 43 68919.0 44 71890.5 45 73312.0 46 72746.0 47 72483.0 48 73285.5 49 71820.5 50 67957.5 51 63343.0 52 59470.0 53 56621.0 54 53307.5 55 48313.5 56 42603.5 57 37344.0 58 33260.5 59 29115.5 60 24041.0 61 19210.0 62 14800.5 63 11131.5 64 8174.0 65 6076.5 66 4408.5 67 3110.0 68 2173.0 69 1492.0 70 1021.0 71 679.0 72 521.5 73 406.5 74 304.5 75 224.5 76 161.5 77 106.5 78 66.0 79 54.5 80 41.0 81 26.5 82 16.5 83 11.0 84 6.5 85 5.5 86 8.0 87 6.0 88 3.5 89 2.5 90 2.5 91 4.5 92 3.5 93 1.0 94 0.0 95 0.0 96 1.5 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3351911138392477 2 0.2844045814393617 3 0.28253783322141995 4 0.28215350270596135 5 0.2857222860637912 6 0.24503815578453114 7 0.24712452143987781 8 0.24778337375209256 9 0.2584348194662308 10-11 0.26587436015832216 12-13 0.25780341933369166 14-15 0.24657547784636552 16-17 0.24207332037956483 18-19 0.22908843939299936 20-21 0.2356220581557955 22-23 0.23238270095407307 24-25 0.23378276211752938 26-27 0.23079047453288745 28-29 0.24885400875944147 30-31 0.26334875962816573 32-33 0.2540424707181326 34-35 0.25055604389932956 36-37 0.23765351945179095 38-39 0.2249980646213329 40-41 0.2212096638260981 42-43 0.23474358840617585 44-45 0.23177875300120954 46-47 0.24429694693328957 48-49 0.25063840043835645 50-51 0.26565474272091727 52-53 0.28193388526855645 54-55 0.2519286528831102 56-57 0.22357055127820094 58-59 0.21149159222093072 60-61 0.21538980173486794 62-63 0.2212096638260981 64-65 0.21204063581444305 66-67 0.21695457597637793 68-69 0.2176957848276195 70-71 0.21942527214718321 72-73 0.2218959683179885 74-75 0.22557456039452076 76-77 0.2383123717640057 78-79 0.23842218048270813 80-81 0.2299120047832678 82-83 0.2219508726773397 84-85 0.20764828706634478 86-87 0.2118484705567137 88-89 0.22217049011474463 90-91 0.2210998551073957 92-93 0.22502551680100846 94-95 0.22044100279518092 96-97 0.22906098721332374 98-99 0.23400237955493428 100-101 0.2154721582738948 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1821349.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.42913142497199 #Duplication Level Percentage of deduplicated Percentage of total 1 78.25998375012568 47.291828433525176 2 12.587738011377581 15.213321492653028 3 4.034594574769352 7.314211374156486 4 1.7856518364231553 4.316215580098298 5 0.9056618758369818 2.736418026076982 6 0.5367711961460967 1.946197029423114 7 0.3678347898971131 1.5559555802958727 8 0.25940758076220094 1.254061983241046 9 0.1814091412407099 0.9866157153953546 >10 0.9832077824853274 10.805754489084945 >50 0.06974299762882319 2.8680599096188777 >100 0.027269523230689787 2.620619272020728 >500 2.726025285998317E-4 0.10345128169294433 >1k 3.634700381331081E-4 0.6447035553911132 >5k 9.086750953327703E-5 0.3425862773260502 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6239 0.3425482979923123 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4335 0.23801039778757394 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3208 0.17613318479873982 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3119 0.17124669681648053 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 5.490435935122813E-5 0.0 0.0 0.0 0.0 3 5.490435935122813E-5 0.0 0.0 0.0 0.0 4 5.490435935122813E-5 0.0 0.0 5.490435935122813E-5 0.0 5 5.490435935122813E-5 0.0 0.0 5.490435935122813E-5 5.490435935122813E-5 6 5.490435935122813E-5 0.0 0.0 5.490435935122813E-5 5.490435935122813E-5 7 5.490435935122813E-5 0.0 0.0 5.490435935122813E-5 5.490435935122813E-5 8 5.490435935122813E-5 0.0 0.0 5.490435935122813E-5 5.490435935122813E-5 9 5.490435935122813E-5 0.0 0.0 5.490435935122813E-5 5.490435935122813E-5 10-11 5.490435935122813E-5 0.0 0.0 5.490435935122813E-5 5.490435935122813E-5 12-13 5.490435935122813E-5 0.0 0.0 5.490435935122813E-5 5.490435935122813E-5 14-15 5.490435935122813E-5 0.0 0.0 5.490435935122813E-5 5.490435935122813E-5 16-17 5.490435935122813E-5 0.0 0.0 5.490435935122813E-5 5.490435935122813E-5 18-19 1.372608983780703E-4 0.0 0.0 8.23565390268422E-5 5.490435935122813E-5 20-21 1.6471307805368437E-4 0.0 0.0 1.9216525772929843E-4 1.6471307805368437E-4 22-23 1.6471307805368437E-4 0.0 0.0 3.2942615610736874E-4 1.6471307805368437E-4 24-25 2.1961743740491252E-4 0.0 0.0 3.843305154585969E-4 1.6471307805368437E-4 26-27 2.1961743740491252E-4 0.0 0.0 5.215914138366673E-4 1.6471307805368437E-4 28-29 3.2942615610736874E-4 0.0 0.0 9.333741089708782E-4 2.1961743740491252E-4 30-31 3.843305154585969E-4 0.0 0.0 0.002251078733400353 2.470696170805266E-4 32-33 3.843305154585969E-4 0.0 0.0 0.0041452791310177236 2.745217967561406E-4 34-35 3.843305154585969E-4 0.0 0.0 0.006341453505066849 2.745217967561406E-4 36-37 3.843305154585969E-4 0.0 0.0 0.008674888777494043 2.745217967561406E-4 38-39 3.843305154585969E-4 0.0 0.0 0.011118132768623695 3.2942615610736874E-4 40-41 3.843305154585969E-4 0.0 0.0 0.014384942150021769 3.2942615610736874E-4 42-43 3.843305154585969E-4 0.0 0.0 0.021495056686005813 3.2942615610736874E-4 44-45 3.843305154585969E-4 0.0 0.0 0.029977780205770556 3.2942615610736874E-4 46-47 3.843305154585969E-4 0.0 0.0 0.05070417586085918 3.2942615610736874E-4 48-49 3.843305154585969E-4 0.0 0.0 0.09248639332714378 3.2942615610736874E-4 50-51 3.843305154585969E-4 0.0 0.0 0.13026059256078873 3.2942615610736874E-4 52-53 3.843305154585969E-4 0.0 0.0 0.17149376643356107 3.843305154585969E-4 54-55 3.843305154585969E-4 0.0 0.0 0.22450392538717182 3.843305154585969E-4 56-57 4.3923487480982505E-4 0.0 0.0 0.26815289107139817 3.843305154585969E-4 58-59 4.3923487480982505E-4 0.0 0.0 0.31633146640210086 3.843305154585969E-4 60-61 4.3923487480982505E-4 0.0 0.0 0.3936917087279813 3.843305154585969E-4 62-63 4.3923487480982505E-4 0.0 0.0 0.4515059991248245 4.3923487480982505E-4 64-65 4.3923487480982505E-4 0.0 0.0 0.5212345355008843 4.3923487480982505E-4 66-67 4.3923487480982505E-4 0.0 0.0 0.6534167806389659 4.3923487480982505E-4 68-69 4.3923487480982505E-4 0.0 0.0 0.8029488033320358 4.3923487480982505E-4 70-71 4.3923487480982505E-4 0.0 0.0 0.9347741701343345 4.666870544854391E-4 72-73 4.3923487480982505E-4 0.0 0.0 1.1009971180701776 5.215914138366673E-4 74-75 4.3923487480982505E-4 0.0 0.0 1.2602746645480905 5.490435935122812E-4 76-77 4.3923487480982505E-4 0.0 0.0 1.3986061979335096 5.490435935122812E-4 78-79 4.3923487480982505E-4 0.0 0.0 1.5708137210386368 5.490435935122812E-4 80-81 6.039479528635094E-4 0.0 0.0 1.804047439562654 5.490435935122812E-4 82-83 6.039479528635094E-4 0.0 0.0 2.0266297123725328 5.490435935122812E-4 84-85 6.039479528635094E-4 0.0 0.0 2.2765818083189986 5.490435935122812E-4 86-87 6.039479528635094E-4 0.0 0.0 2.7241346935705346 5.490435935122812E-4 88-89 6.039479528635094E-4 0.0 0.0 3.3020305279218864 5.490435935122812E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3895 0.0 46.994717 1 GTATCAA 6775 0.0 38.947487 1 ATCAACG 8545 0.0 30.696127 3 TCAACGC 8600 0.0 30.554224 4 TATCAAC 8755 0.0 30.015766 2 CAACGCA 8790 0.0 29.894604 5 AACGCAG 9045 0.0 29.09393 6 CGCAGAG 10530 0.0 24.675848 8 ACGCAGA 10610 0.0 24.624102 7 GTACATG 7500 0.0 24.469313 1 TACATGG 7395 0.0 23.776285 2 CCGTCCG 120 1.7108282E-4 23.751064 9 ACATGGG 7500 0.0 22.491772 3 GCAGAGT 11290 0.0 22.089123 9 GAGTACT 6010 0.0 20.785408 12-13 AGAGTAC 9995 0.0 19.84097 10-11 GTACTTT 6315 0.0 19.667887 14-15 CATGGGA 4615 0.0 19.150753 4 AGTACTT 6425 0.0 19.073212 12-13 CAGAGTA 10950 0.0 18.978117 10-11 >>END_MODULE