FastQCFastQC Report
Thu 26 May 2016
SRR937351_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937351_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1821349
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68930.3784557490080155No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56530.3103743434124926No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38460.21116216606482338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40750.046.412341
GTATCAA70400.039.555141
ATCAACG88900.030.9359913
TCAACGC90300.030.4037634
TATCAAC91250.030.3474982
CAACGCA92900.029.6039755
AACGCAG95750.028.7220276
GTACATG74400.025.8039651
ACGCAGA108200.025.1119147
CGCAGAG109350.024.630628
TACATGG75500.024.5989762
ACATGGG74700.023.9722023
GGACCGT1401.7808043E-523.7489976
GCAGAGT117600.022.660359
GTACCGT1503.035458E-522.1657336
GAGTACT68100.021.86761912-13
AGAGTAC106050.020.51473210-11
GTACTTT72700.020.48228514-15
AGTACTT73550.019.95661412-13
GTCTACG1707.931194E-519.567131
Produced by FastQC (version 0.11.5)