##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937351_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1821349 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26826654309526 33.0 31.0 34.0 31.0 34.0 2 32.48069370559953 34.0 31.0 34.0 31.0 34.0 3 32.550146622091646 34.0 31.0 34.0 31.0 34.0 4 36.16609886408371 37.0 35.0 37.0 35.0 37.0 5 36.09303378978988 37.0 35.0 37.0 35.0 37.0 6 36.12796449225272 37.0 35.0 37.0 35.0 37.0 7 36.06995693851096 37.0 35.0 37.0 35.0 37.0 8 36.04012081155231 37.0 35.0 37.0 35.0 37.0 9 37.75113720654306 39.0 37.0 39.0 35.0 39.0 10-11 37.672701662339286 39.0 37.0 39.0 35.0 39.0 12-13 37.56734953048537 39.0 37.0 39.0 35.0 39.0 14-15 38.81673666057411 40.0 38.0 41.0 35.0 41.0 16-17 38.76620927675036 40.0 38.0 41.0 35.0 41.0 18-19 38.74599129546287 40.0 38.0 41.0 35.0 41.0 20-21 38.635624748469404 40.0 38.0 41.0 34.0 41.0 22-23 38.58037311904528 40.0 38.0 41.0 34.0 41.0 24-25 38.619298937216314 40.0 38.0 41.0 34.0 41.0 26-27 38.478526905057734 40.0 38.0 41.0 34.0 41.0 28-29 38.334857844377986 40.0 38.0 41.0 34.0 41.0 30-31 38.40718747477831 40.0 38.0 41.0 34.0 41.0 32-33 38.33118666438997 40.0 38.0 41.0 34.0 41.0 34-35 38.213853852282014 40.0 38.0 41.0 34.0 41.0 36-37 38.06605433664828 40.0 38.0 41.0 33.5 41.0 38-39 37.96467261354084 40.0 37.5 41.0 33.0 41.0 40-41 37.792821694249696 40.0 37.0 41.0 33.0 41.0 42-43 37.60963302475253 40.0 37.0 41.0 33.0 41.0 44-45 37.33090857380985 40.0 37.0 41.0 32.5 41.0 46-47 37.014375608408926 40.0 37.0 41.0 31.5 41.0 48-49 36.72050771159179 39.5 36.0 41.0 31.0 41.0 50-51 36.49143464541942 39.0 36.0 41.0 30.5 41.0 52-53 36.26524954854891 39.0 36.0 40.5 30.0 41.0 54-55 36.06070692656927 39.0 35.0 40.0 29.5 41.0 56-57 35.77530445839869 39.0 35.0 40.0 29.0 41.0 58-59 35.51544267463292 38.0 35.0 40.0 28.0 41.0 60-61 35.18200548055315 38.0 34.0 40.0 28.0 41.0 62-63 34.810664787473456 38.0 34.0 40.0 27.0 41.0 64-65 34.40203332804421 37.0 34.0 40.0 26.0 41.0 66-67 33.973399112416125 36.5 33.0 39.0 26.0 40.5 68-69 33.515961520828796 36.0 33.0 39.0 25.5 40.0 70-71 33.07568730649645 35.5 32.0 38.5 24.5 40.0 72-73 32.590212529284614 35.0 32.0 38.0 23.5 40.0 74-75 32.1000387624777 35.0 31.0 37.0 22.0 39.0 76-77 30.76583839780295 33.5 29.5 35.5 21.5 38.5 78-79 31.425743501108244 34.0 31.0 36.0 22.0 38.5 80-81 31.239081856360315 34.0 31.0 36.0 21.0 37.5 82-83 30.83270394636064 34.0 31.0 35.0 20.0 37.0 84-85 30.392139837010916 34.0 30.5 35.0 18.0 36.5 86-87 30.009251659072476 34.0 30.0 35.0 16.0 36.0 88-89 29.559122386758386 34.0 29.5 35.0 9.0 36.0 90-91 28.999726850812227 34.0 29.0 35.0 4.5 35.0 92-93 28.491311110610873 33.5 29.0 35.0 2.0 35.0 94-95 28.004931509556926 33.0 28.0 35.0 2.0 35.0 96-97 27.342338563339588 33.0 26.5 35.0 2.0 35.0 98-99 26.36922577715748 32.5 25.0 34.0 2.0 35.0 100-101 23.38611161287595 29.5 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18386915248520808 1101 2 0.1594851358384375 1101 3 0.10323989072304585 1101 4 0.09199301091129541 1101 5 0.05226682297153218 1101 6 0.11806524782518579 1101 7 0.15255734756966177 1101 8 0.10657801326627236 1101 9 0.20450010171370536 1101 10-11 0.12933282420593883 1101 12-13 0.23271248109854525 1101 14-15 0.21068095421147603 1101 16-17 0.07857033998809726 1101 18-19 0.0779694010738794 1101 20-21 0.18475026180385612 1101 22-23 0.20246302370458835 1101 24-25 0.18364824935828494 1101 26-27 0.08363750545159832 1101 28-29 -0.008566842511243067 1101 30-31 0.19313492857305192 1101 32-33 0.24818172131364236 1101 34-35 0.19602561799945306 1101 36-37 0.2666422443404173 1101 38-39 0.25442122661075217 1101 40-41 0.18248330492889409 1101 42-43 0.3039779114136465 1101 44-45 0.3366559107333842 1101 46-47 0.24851573775184477 1101 48-49 0.35905942246520084 1101 50-51 0.2572452546657402 1101 52-53 0.18210066708402195 1101 54-55 0.25525365283153434 1101 56-57 0.37550435461934484 1101 58-59 0.4399725644733792 1101 60-61 0.33973974696433373 1101 62-63 0.39277648689375155 1101 64-65 0.4134025327718831 1101 66-67 0.20724881612687085 1101 68-69 0.2926819361856161 1101 70-71 0.3029701195407597 1101 72-73 0.3709297056639258 1101 74-75 0.29484245116822194 1101 76-77 0.3180130864484383 1101 78-79 0.4565670874615968 1101 80-81 0.34452726705728765 1101 82-83 0.37964811519774244 1101 84-85 0.35543928776415257 1101 86-87 0.5396576235405135 1101 88-89 0.7732626406336927 1101 90-91 0.9556789837879371 1101 92-93 0.8355889487820711 1101 94-95 0.8611340432889598 1101 96-97 0.9980456359225016 1101 98-99 1.4979370205922464 1101 100-101 2.1126325744678027 1102 1 0.1911035196950266 1102 2 0.15892681923617857 1102 3 0.11907125819843145 1102 4 0.08293537704451381 1102 5 0.08804643339260565 1102 6 0.11172690557442166 1102 7 0.14842120435144324 1102 8 0.046749564929378096 1102 9 0.14850100625232443 1102 10-11 0.06566083356678121 1102 12-13 0.024998989224002344 1102 14-15 0.1191181516592863 1102 16-17 0.14536004641035305 1102 18-19 0.1402682286090169 1102 20-21 0.20736678911043782 1102 22-23 0.21109064325947458 1102 24-25 0.014263983849311046 1102 26-27 0.05784224782686209 1102 28-29 0.19802381761972043 1102 30-31 0.1070709022948293 1102 32-33 0.04559539209444097 1102 34-35 0.09362769449510466 1102 36-37 0.11671564101733622 1102 38-39 0.1628535697809994 1102 40-41 0.12147153308173131 1102 42-43 0.15748238616139787 1102 44-45 -0.006156830953635506 1102 46-47 0.043613918129622675 1102 48-49 -0.011517770555514062 1102 50-51 -0.07896268227225534 1102 52-53 0.0036441301943455073 1102 54-55 0.1343666822203815 1102 56-57 0.11024683743445252 1102 58-59 0.13687386252213685 1102 60-61 0.20914765653915168 1102 62-63 0.23580804447757941 1102 64-65 0.24219410443161848 1102 66-67 0.172380465628315 1102 68-69 0.09202499559927446 1102 70-71 0.10168996439505662 1102 72-73 0.2776540152586975 1102 74-75 0.045240576610524386 1102 76-77 0.12364377958251893 1102 78-79 0.06849515274371143 1102 80-81 0.06055299980675244 1102 82-83 0.1622147913140708 1102 84-85 0.1489718516023828 1102 86-87 0.223912482971091 1102 88-89 0.29332230792001823 1102 90-91 0.45085891020007907 1102 92-93 0.40929271477542173 1102 94-95 0.4887147639505294 1102 96-97 0.5383114949967016 1102 98-99 0.8357871969742874 1102 100-101 1.5368932978656886 1103 1 0.07748248084701004 1103 2 0.06622545784175315 1103 3 0.08528042653349388 1103 4 0.08024952892290571 1103 5 0.026030496891522148 1103 6 0.01556602012218633 1103 7 0.027668738756325695 1103 8 0.036032012607634556 1103 9 0.0812405866264001 1103 10-11 0.028820862471071962 1103 12-13 0.06309778917005815 1103 14-15 0.02886602504431579 1103 16-17 0.09093660776025558 1103 18-19 0.05637568760953826 1103 20-21 0.13126544000920148 1103 22-23 0.07954341064937154 1103 24-25 0.10515668661739141 1103 26-27 0.024681136387869174 1103 28-29 0.06116811307463621 1103 30-31 0.014401879331337852 1103 32-33 0.07183049845041722 1103 34-35 0.14802338172574991 1103 36-37 0.194391252458324 1103 38-39 0.23085372145817473 1103 40-41 0.1446214919668165 1103 42-43 0.22844452405123405 1103 44-45 0.2454220183975977 1103 46-47 0.33273531583483873 1103 48-49 0.39890370546527265 1103 50-51 0.2970890047846879 1103 52-53 0.2987791763993144 1103 54-55 0.17793572270625901 1103 56-57 0.34886707355546065 1103 58-59 0.22757436950922028 1103 60-61 0.29807780644573967 1103 62-63 0.23932742903519255 1103 64-65 0.265962927615476 1103 66-67 0.22600828713729726 1103 68-69 0.224319834630343 1103 70-71 0.3216033738503583 1103 72-73 0.07560005854846708 1103 74-75 0.056181057690174896 1103 76-77 0.09060781710672217 1103 78-79 0.0891406033610167 1103 80-81 0.26625882925616295 1103 82-83 0.1522041739114961 1103 84-85 0.2334877674619129 1103 86-87 0.2628483360619569 1103 88-89 0.47340776700188414 1103 90-91 0.6527063749435449 1103 92-93 0.7006676347702943 1103 94-95 0.7673108815651766 1103 96-97 0.9195360313492245 1103 98-99 1.140895399988075 1103 100-101 1.913227315479201 1104 1 0.059013348227523466 1104 2 0.08947502612544156 1104 3 0.028715518691164732 1104 4 0.0649145358652845 1104 5 0.04579142139554904 1104 6 -0.009380871386092338 1104 7 0.1040697689670651 1104 8 0.018548509139129976 1104 9 0.12765378277734385 1104 10-11 0.058697242553300555 1104 12-13 0.10034221714259672 1104 14-15 0.011891449979387403 1104 16-17 0.0813676795178182 1104 18-19 0.06063242386108669 1104 20-21 0.11392964663144767 1104 22-23 0.018279979102253208 1104 24-25 -0.06688528453349107 1104 26-27 0.03517392410697795 1104 28-29 -0.05850660244915673 1104 30-31 -0.0364815612932361 1104 32-33 0.05474715504594485 1104 34-35 0.04640958184337762 1104 36-37 0.0053236791202664335 1104 38-39 0.07583332403746823 1104 40-41 0.14985042917928837 1104 42-43 0.13103106202213866 1104 44-45 -0.014044184190815656 1104 46-47 0.07819994463937974 1104 48-49 0.09893539599383416 1104 50-51 0.06870234810859444 1104 52-53 0.07910204380130637 1104 54-55 0.1384165593362141 1104 56-57 0.03714641213652925 1104 58-59 0.014639531144347018 1104 60-61 0.09739753433689913 1104 62-63 0.14871534527551233 1104 64-65 0.09912572293495003 1104 66-67 0.07742133557815123 1104 68-69 0.24999050558328406 1104 70-71 0.10526180366523619 1104 72-73 0.2582207578348914 1104 74-75 0.14535095383655516 1104 76-77 0.19094459611362424 1104 78-79 0.10429297303371854 1104 80-81 0.0892860267123261 1104 82-83 0.05265160120455903 1104 84-85 0.14356186421470696 1104 86-87 0.27879431176767255 1104 88-89 0.2901290864021284 1104 90-91 0.5233835048559108 1104 92-93 0.49765354322763855 1104 94-95 0.48656099771902106 1104 96-97 0.4505846924243926 1104 98-99 0.6622005663499237 1104 100-101 1.4844893773509291 1105 1 -0.020653001070819244 1105 2 0.05382283421419487 1105 3 0.006420393744633657 1105 4 0.0305387165720461 1105 5 0.0024878930876752747 1105 6 -0.07668020491260563 1105 7 -0.07399456021288131 1105 8 0.05638778277962331 1105 9 0.1263325646349145 1105 10-11 -0.006861739660273258 1105 12-13 -0.02716395774115199 1105 14-15 0.05251632061729339 1105 16-17 0.059176044243017145 1105 18-19 0.0364641358562281 1105 20-21 0.11700328988185049 1105 22-23 0.022302277676850224 1105 24-25 0.014295463627846061 1105 26-27 -0.11477743574730681 1105 28-29 0.03895824555900873 1105 30-31 0.042137817492758245 1105 32-33 0.08953003371465229 1105 34-35 0.18417848250709312 1105 36-37 0.19516340811530597 1105 38-39 0.16455156995652231 1105 40-41 0.06031633968141392 1105 42-43 -0.07424932599514023 1105 44-45 0.11369829211004401 1105 46-47 0.0889818436058647 1105 48-49 0.22150683836388652 1105 50-51 0.24483831973862635 1105 52-53 0.27356431146699123 1105 54-55 0.27514711362689326 1105 56-57 0.319787054600404 1105 58-59 0.47067366893739404 1105 60-61 0.5106369680414176 1105 62-63 0.4298743606057869 1105 64-65 0.46285224802774394 1105 66-67 0.5354424750684288 1105 68-69 0.4047282627551283 1105 70-71 0.44527712013665877 1105 72-73 0.31881223580538887 1105 74-75 0.3668826699712753 1105 76-77 0.4539377243994238 1105 78-79 0.5372592629979813 1105 80-81 0.4057384697585107 1105 82-83 0.34179484470886123 1105 84-85 0.5142118483462887 1105 86-87 0.6958611384983691 1105 88-89 0.7888682372348761 1105 90-91 0.9881676943937912 1105 92-93 0.9114431970201871 1105 94-95 0.9686279624359422 1105 96-97 1.2760062403539116 1105 98-99 1.2244513287238377 1105 100-101 1.794815231830654 1106 1 -0.08011586565152129 1106 2 0.030495928467651368 1106 3 -0.014102678583292061 1106 4 0.10409596858103498 1106 5 0.06029316502657167 1106 6 0.01767373110581616 1106 7 -0.03209115996090617 1106 8 -0.10275886356474473 1106 9 -0.08429260968959085 1106 10-11 -0.1976390860039814 1106 12-13 -0.2800821773142559 1106 14-15 -0.06207893175332657 1106 16-17 -0.09316160611529511 1106 18-19 -0.02043977318536605 1106 20-21 -0.09097398222981212 1106 22-23 0.11902281747700982 1106 24-25 0.16992633712811056 1106 26-27 0.10932654547652731 1106 28-29 0.17031207615915633 1106 30-31 0.07650701353927758 1106 32-33 0.138921975219219 1106 34-35 -0.2543859035375746 1106 36-37 0.23581212951602737 1106 38-39 0.09166471232170892 1106 40-41 -0.024801509170728764 1106 42-43 -0.13107279869290522 1106 44-45 -0.017248140762298192 1106 46-47 0.05959366128787735 1106 48-49 0.1985151298044343 1106 50-51 -0.005435108925041732 1106 52-53 -0.030365283031343893 1106 54-55 -0.01790142929481675 1106 56-57 0.06973617563890144 1106 58-59 0.09510988245919094 1106 60-61 0.15560842457797008 1106 62-63 0.07593696954715057 1106 64-65 0.07860106188908134 1106 66-67 0.008751335255240633 1106 68-69 0.08643716628056097 1106 70-71 0.2192601448652951 1106 72-73 0.22975346365016946 1106 74-75 0.21577974951888734 1106 76-77 0.18083712022090737 1106 78-79 0.24377953002833763 1106 80-81 0.25748696736500065 1106 82-83 0.11991096148010172 1106 84-85 0.29342323542391213 1106 86-87 0.30663433765765546 1106 88-89 0.5906104024238559 1106 90-91 0.5407557680723869 1106 92-93 0.7600242962546631 1106 94-95 0.41397306820927326 1106 96-97 0.768533896180795 1106 98-99 0.9637176065141801 1106 100-101 1.3645442080214956 1107 1 0.038725179412473665 1107 2 0.0581329042700105 1107 3 0.01892525720504068 1107 4 0.04630896780137306 1107 5 -0.06185431427488908 1107 6 -0.011207036925938496 1107 7 0.024157214922688297 1107 8 -0.02160838360457973 1107 9 -0.03372803902768595 1107 10-11 -0.08545278693640768 1107 12-13 -0.005404231369880108 1107 14-15 -0.0297407316750693 1107 16-17 -0.05867521849814494 1107 18-19 -0.016239288679351205 1107 20-21 0.012803787988119097 1107 22-23 0.004715785017658902 1107 24-25 -0.0779338806787706 1107 26-27 -0.08294320226163876 1107 28-29 -0.09408817108731427 1107 30-31 -0.0654345079337375 1107 32-33 -0.0010341936381053074 1107 34-35 -0.02464641753093133 1107 36-37 -0.006912272824102672 1107 38-39 -0.07118612631295917 1107 40-41 -0.07768476160385518 1107 42-43 -0.06150458677554127 1107 44-45 -0.09844447710584348 1107 46-47 -0.09968057838643318 1107 48-49 -0.20210523444843886 1107 50-51 -0.09542970364115178 1107 52-53 -0.05609339983799799 1107 54-55 -0.0487021971355901 1107 56-57 -0.06476208888101098 1107 58-59 -0.13598778805642553 1107 60-61 0.07042208552283569 1107 62-63 0.0394250428091496 1107 64-65 0.24386876071231 1107 66-67 0.13140651733492348 1107 68-69 0.28372207049841336 1107 70-71 0.2522911642715968 1107 72-73 0.2476317832892505 1107 74-75 0.13125240946565953 1107 76-77 0.18679059180775326 1107 78-79 0.30928661707768157 1107 80-81 0.2452011370670455 1107 82-83 0.09285161695383337 1107 84-85 0.19427666151247536 1107 86-87 0.4313905577627253 1107 88-89 0.5570243317827455 1107 90-91 0.4671677119791866 1107 92-93 0.569502363076861 1107 94-95 0.6477156871717717 1107 96-97 0.5970756850731256 1107 98-99 0.45979912384520816 1107 100-101 1.0602532839845331 1108 1 -0.1076233201950032 1108 2 -0.06305366072756868 1108 3 -0.06064842496057565 1108 4 -0.011227612878670357 1108 5 -0.14406608969692059 1108 6 -0.13563935277039008 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2316 54-55 -0.17043507120364865 2316 56-57 -0.05829176313357465 2316 58-59 -0.3881650363755398 2316 60-61 -0.010139862644365394 2316 62-63 -0.04709331526735383 2316 64-65 -0.05735110000396304 2316 66-67 0.06675339799112834 2316 68-69 -0.11174744802512038 2316 70-71 -0.16059884771141952 2316 72-73 -0.0516552524943279 2316 74-75 0.273233567556197 2316 76-77 0.27682613974231174 2316 78-79 0.21481133768294924 2316 80-81 0.02906568389354902 2316 82-83 0.17617366920779176 2316 84-85 0.30382596985696253 2316 86-87 0.2501236674799969 2316 88-89 0.2877850776375759 2316 90-91 0.4487319213760763 2316 92-93 0.5048316953873773 2316 94-95 0.8049939888930169 2316 96-97 0.9795318518124922 2316 98-99 1.2167369876600205 2316 100-101 1.6180926313866273 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 7.0 5 34.0 6 45.0 7 134.0 8 323.0 9 700.0 10 1134.0 11 1903.0 12 3070.0 13 4435.0 14 5637.0 15 8046.0 16 11292.0 17 15942.0 18 14546.0 19 8927.0 20 8063.0 21 8044.0 22 8750.0 23 9831.0 24 11324.0 25 13766.0 26 16647.0 27 20751.0 28 25973.0 29 32863.0 30 41265.0 31 52800.0 32 68668.0 33 91067.0 34 126347.0 35 182212.0 36 281024.0 37 416302.0 38 306505.0 39 22962.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.270606361037345 20.318244109820032 15.18162818607998 22.229521343062647 2 15.510114217664178 21.2050202405143 39.97354705061693 23.311318491204585 3 19.188195123504613 25.2742884532289 30.414983619284385 25.122532803982107 4 12.908069787833085 17.43290275504585 37.66993585523697 31.989091601884095 5 13.341228688580395 38.35286857253295 34.48826238494633 13.81764035394032 6 31.587905448104674 37.27215377173732 17.437624529950053 13.70231625020795 7 28.110702561672696 33.31327494071702 20.965943374938025 17.61007912267226 8 26.292544701756775 35.878022279090935 19.761122113334675 18.06831090581761 9 26.312035749326462 16.90735822733589 20.567008299892002 36.213597723445645 10-11 24.473892702606694 27.561192281105928 27.668722468895307 20.29619254739207 12-13 25.485690973567266 24.713856533893974 28.412534169610854 21.38791832292791 14-15 22.45077686923264 26.13109843308449 26.57554922203268 24.842575475650193 16-17 21.6041516480367 29.643824440016715 27.148778185839177 21.603245726107406 18-19 21.598496499023526 27.976653568316674 29.691261806496172 20.733588126163628 20-21 22.289098910667963 27.045637414757383 29.915274175053554 20.749989499521096 22-23 21.969727125757036 27.127294369757106 29.768079267196264 21.134899237289602 24-25 21.89498605017107 27.127784715555535 29.917146553775954 21.060082680497445 26-27 21.793496687123636 27.516516339268186 29.624595584753223 21.065391388854955 28-29 21.571740441031935 27.60729577208042 29.743163302172 21.077800484715645 30-31 21.790406571423627 27.317434603798684 29.78097725274324 21.111181572034443 32-33 21.58914559499622 27.402272004155176 29.6963203261768 21.3122620746718 34-35 21.960892722921308 27.348053558104464 29.597238091107197 21.093815627867038 36-37 22.01915229293021 27.242974093096407 29.49130822574592 21.24656538822746 38-39 21.768876678152665 27.497188895257796 29.64771147523702 21.086222951352514 40-41 22.00017679203711 27.399032255762073 29.489213764083654 21.11157718811716 42-43 21.879521432999464 27.4089920474943 29.3108011423319 21.400685377174337 44-45 21.876532174778145 27.635697496745543 29.206099435089044 21.281670893387265 46-47 22.060077448089302 27.829098102560245 28.726400047437366 21.384424401913087 48-49 22.162830956615124 27.957793920879524 28.491299580695404 21.388075541809943 50-51 22.122531156851323 28.019698586048037 28.37443565181632 21.483334605284323 52-53 22.397004197713947 28.023000540533566 28.146041243617027 21.433954018135466 54-55 22.161019112756534 28.00608230492893 28.242582832834344 21.590315749480194 56-57 22.198683503271475 28.30069909720762 28.071006709861756 21.42961068965915 58-59 22.352662779072 27.968637531851392 28.17656034071449 21.50213934836212 60-61 22.213507680296306 27.91787845163118 28.45918053047494 21.409433337597573 62-63 22.15777975555481 27.94360114398723 28.56476161350735 21.333857486950606 64-65 22.166097765996522 27.93989509973102 28.408064571918946 21.485942562353507 66-67 22.08794141046005 28.12717386947806 28.1299465396253 21.654938180436588 68-69 22.06658361467242 28.038338616047774 28.278874614365506 21.616203154914295 70-71 22.071552459193708 27.956668381512824 28.267729029417204 21.70405012987626 72-73 22.129012793556853 27.880142520931628 28.13602463891412 21.854820046597393 74-75 22.047284732360463 28.179744793556864 27.94824056235241 21.824729911730262 76-77 22.096835916674948 28.035016902307024 28.07018314447149 21.79796403654654 78-79 22.049206384937758 28.0495665575351 27.991395388802477 21.909831668724667 80-81 22.014424473288756 28.18721178642863 27.986453996460863 21.81190974382175 82-83 22.082584686845006 28.10468102512295 28.035913069617603 21.776821218414437 84-85 22.088438416303543 27.976252733897695 27.880444495322553 22.05486435447621 86-87 22.03397042521779 27.920815834856473 27.94266776987826 22.102545970047476 88-89 22.229402492328486 27.70141801488896 27.94651107503285 22.1226684177497 90-91 22.05413199588398 27.655781038426774 28.10461141085605 22.18547555483319 92-93 21.968679978938432 27.559191732182086 28.135612111389115 22.336516177490367 94-95 21.823220041848103 27.692523508674068 28.12876609589925 22.355490353578585 96-97 21.872030017311346 27.666443937982233 28.108561291657995 22.352964753048425 98-99 21.662083975257858 27.69819682806013 27.965444237393665 22.67427495928835 100-101 21.78914485370872 27.77365334762485 28.073275504673756 22.363926293992677 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 162.0 1 119.0 2 114.5 3 180.0 4 183.5 5 186.5 6 335.5 7 721.5 8 1198.0 9 1565.5 10 1656.5 11 1653.0 12 1780.5 13 1921.5 14 2084.5 15 2264.5 16 2376.0 17 2478.0 18 2655.0 19 2862.0 20 3226.5 21 3702.5 22 4354.5 23 5242.5 24 6345.0 25 7912.0 26 10109.5 27 12889.0 28 15749.5 29 18998.5 30 23190.0 31 27471.0 32 31713.5 33 35803.5 34 40624.5 35 45168.0 36 49425.5 37 54194.0 38 57673.5 39 59638.0 40 61146.5 41 63891.0 42 66335.0 43 69286.5 44 72978.5 45 74074.5 46 72805.0 47 72500.5 48 73643.5 49 71865.0 50 68326.5 51 63998.5 52 59270.5 53 56234.0 54 53413.0 55 48497.5 56 42101.5 57 36441.0 58 32428.5 59 28600.0 60 23611.0 61 18593.0 62 14122.5 63 10585.5 64 7767.0 65 5668.5 66 3999.5 67 2879.5 68 2098.0 69 1363.0 70 890.0 71 594.5 72 413.5 73 341.5 74 260.5 75 183.0 76 122.0 77 66.0 78 41.5 79 26.0 80 15.0 81 8.5 82 4.0 83 3.0 84 1.5 85 1.0 86 1.0 87 1.0 88 1.0 89 0.5 90 0.0 91 1.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04029979976380144 2 1.0980871870245626E-4 3 0.0 4 0.0 5 0.0013726089837807034 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005682601192852112 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.9216525772929843E-4 22-23 8.51017569944036E-4 24-25 2.7452179675614065E-5 26-27 0.002553052709832108 28-29 0.004117826951342109 30-31 4.6668705448543914E-4 32-33 3.019739764317547E-4 34-35 0.0 36-37 2.7452179675614065E-5 38-39 5.490435935122813E-5 40-41 0.0 42-43 0.008894506214898956 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.7452179675614065E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.372608983780703E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.5687833578298283E-4 84-85 1.372608983780703E-4 86-87 0.0 88-89 0.0 90-91 0.00938864544906001 92-93 0.0012078959057270188 94-95 0.0 96-97 0.0 98-99 1.647130780536844E-4 100-101 0.012188767775972643 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1821349.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.75868898413088 #Duplication Level Percentage of deduplicated Percentage of total 1 77.02787738389887 45.26057090308283 2 13.21982408148429 15.535590632577184 3 4.255922472193606 7.5021727465259245 4 1.8971598704921762 4.458985071336953 5 1.0054059892366298 2.9538168912168787 6 0.5719854470400602 2.016546899164558 7 0.38448958994774274 1.5814472963362807 8 0.2766618584706453 1.3005030476518626 9 0.19316282125665687 1.0214994723765451 >10 1.0611626205374929 11.252748435712295 >50 0.07468030313814003 2.9712867570174377 >100 0.03092045148666564 3.0029025508386917 >500 3.7355540851916074E-4 0.16171387005243384 >1k 1.8677770425958032E-4 0.29176540450247396 >5k 1.8677770425958032E-4 0.6884500216076807 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6893 0.3784557490080155 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5653 0.3103743434124926 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3846 0.21116216606482338 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.490435935122813E-5 7 5.490435935122813E-5 0.0 0.0 0.0 5.490435935122813E-5 8 5.490435935122813E-5 0.0 0.0 0.0 5.490435935122813E-5 9 5.490435935122813E-5 0.0 0.0 0.0 5.490435935122813E-5 10-11 5.490435935122813E-5 0.0 0.0 1.372608983780703E-4 5.490435935122813E-5 12-13 5.490435935122813E-5 0.0 0.0 2.745217967561406E-4 8.23565390268422E-5 14-15 5.490435935122813E-5 0.0 0.0 2.745217967561406E-4 1.372608983780703E-4 16-17 5.490435935122813E-5 0.0 0.0 2.745217967561406E-4 1.6471307805368437E-4 18-19 1.3726089837807034E-4 0.0 0.0 3.0197397643175466E-4 1.9216525772929846E-4 20-21 2.1961743740491252E-4 0.0 0.0 4.666870544854391E-4 2.1961743740491252E-4 22-23 2.1961743740491252E-4 0.0 0.0 6.588523122147375E-4 2.745217967561406E-4 24-25 2.1961743740491252E-4 0.0 0.0 6.863044918903516E-4 2.745217967561406E-4 26-27 2.1961743740491252E-4 0.0 0.0 9.608262886464923E-4 2.745217967561406E-4 28-29 2.1961743740491252E-4 0.0 0.0 0.0014000611634563172 2.745217967561406E-4 30-31 2.1961743740491252E-4 0.0 0.0 0.0028824788659394768 2.745217967561406E-4 32-33 2.1961743740491252E-4 0.0 0.0 0.0048864879822593035 2.745217967561406E-4 34-35 2.1961743740491252E-4 0.0 0.0 0.007055210176632814 2.745217967561406E-4 36-37 2.1961743740491252E-4 0.0 0.0 0.009361193269384397 2.745217967561406E-4 38-39 2.1961743740491252E-4 0.0 0.0 0.011722080721487206 2.745217967561406E-4 40-41 2.1961743740491252E-4 0.0 0.0 0.01507124664191212 2.745217967561406E-4 42-43 2.745217967561406E-4 0.0 0.0 0.02220881335757178 2.745217967561406E-4 44-45 3.2942615610736874E-4 0.0 0.0 0.030856249955390207 2.745217967561406E-4 46-47 3.5687833578298283E-4 0.0 0.0 0.05147283689177637 2.745217967561406E-4 48-49 3.843305154585969E-4 0.0 0.0 0.09325505435806097 2.745217967561406E-4 50-51 3.843305154585969E-4 0.0 0.0 0.13122141884943522 2.745217967561406E-4 52-53 3.843305154585969E-4 0.0 0.0 0.1729761841360442 2.745217967561406E-4 54-55 3.843305154585969E-4 0.0 0.0 0.22656283886284287 2.745217967561406E-4 56-57 3.843305154585969E-4 0.0 0.0 0.27048632634382536 3.0197397643175466E-4 58-59 3.843305154585969E-4 0.0 0.0 0.31907668436966224 3.2942615610736874E-4 60-61 3.843305154585969E-4 0.0 0.0 0.39651928323456953 3.5687833578298283E-4 62-63 3.843305154585969E-4 0.0 0.0 0.4550473303029787 3.843305154585969E-4 64-65 4.3923487480982505E-4 0.0 0.0 0.5255445277099556 3.843305154585969E-4 66-67 4.3923487480982505E-4 0.0 0.0 0.6587150513163595 3.843305154585969E-4 68-69 4.941392341610532E-4 0.0 0.0 0.8097020395322369 3.843305154585969E-4 70-71 4.941392341610532E-4 0.0 0.0 0.9418842846703186 3.843305154585969E-4 72-73 4.941392341610532E-4 0.0 0.0 1.1092602241525376 3.843305154585969E-4 74-75 4.941392341610532E-4 0.0 0.0 1.2700201883329334 3.843305154585969E-4 76-77 4.941392341610532E-4 0.0 0.0 1.4101910177566188 3.843305154585969E-4 78-79 4.941392341610532E-4 0.0 0.0 1.584100576001634 3.843305154585969E-4 80-81 4.941392341610532E-4 0.0 0.0 1.8212325040395883 3.843305154585969E-4 82-83 5.490435935122812E-4 0.0 0.0 2.0469992296918385 4.11782695134211E-4 84-85 5.764957731878953E-4 0.0 0.0 2.3004103002774317 4.3923487480982505E-4 86-87 6.039479528635094E-4 0.0 0.0 2.7572420222593252 4.3923487480982505E-4 88-89 6.039479528635094E-4 0.0 0.0 3.353640625712041 4.666870544854391E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4075 0.0 46.41234 1 GTATCAA 7040 0.0 39.55514 1 ATCAACG 8890 0.0 30.935991 3 TCAACGC 9030 0.0 30.403763 4 TATCAAC 9125 0.0 30.347498 2 CAACGCA 9290 0.0 29.603975 5 AACGCAG 9575 0.0 28.722027 6 GTACATG 7440 0.0 25.803965 1 ACGCAGA 10820 0.0 25.111914 7 CGCAGAG 10935 0.0 24.63062 8 TACATGG 7550 0.0 24.598976 2 ACATGGG 7470 0.0 23.972202 3 GGACCGT 140 1.7808043E-5 23.748997 6 GCAGAGT 11760 0.0 22.66035 9 GTACCGT 150 3.035458E-5 22.165733 6 GAGTACT 6810 0.0 21.867619 12-13 AGAGTAC 10605 0.0 20.514732 10-11 GTACTTT 7270 0.0 20.482285 14-15 AGTACTT 7355 0.0 19.956614 12-13 GTCTACG 170 7.931194E-5 19.56713 1 >>END_MODULE