FastQCFastQC Report
Thu 26 May 2016
SRR937350_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937350_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1770515
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61270.34605750304289995No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45250.25557535519326297No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30370.17153201187225187No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26380.14899619602206138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35150.048.0530131
GTATCAA61850.039.617321
ATCAACG77700.031.2075023
TCAACGC78150.030.7253534
TATCAAC80200.030.2939832
CAACGCA81000.029.7004495
AACGCAG82300.029.3202616
ACGCAGA95650.024.8803637
CGCAGAG96850.024.523048
GTACATG71850.023.9717311
TACATGG70450.023.6884142
ACATGGG71450.022.4918063
GCAGAGT104300.022.0882559
GAGTACT57600.020.5328912-13
AGAGTAC94800.020.16765210-11
CATGGGG30450.019.8312874
AGTACTT61400.019.64891612-13
GTACTTT61500.019.11387414-15
CAGAGTA101700.018.84605410-11
TGGTATC18000.018.2257922
Produced by FastQC (version 0.11.5)