FastQCFastQC Report
Thu 26 May 2016
SRR937350_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937350_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1770515
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71610.404458589732366No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55710.3146542107804791No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40080.22637481184852995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43500.043.3669851
GTATCAA72900.037.479891
ATCAACG92650.029.3257483
TCAACGC93950.028.9199664
TATCAAC95350.028.495342
CAACGCA95800.028.3119055
AACGCAG98400.027.6105426
GTACATG74950.025.0428331
TACATGG74650.024.6888262
ACGCAGA111700.024.0705667
CGCAGAG111950.023.8470828
ACATGGG73800.023.6859043
AGTACTT69400.022.07462512-13
GAGTACT66250.021.97696112-13
GCAGAGT121800.021.450559
AGAGTAC104500.021.36508610-11
GTACTTT69850.020.73993714-15
CATGGGA47450.019.0202354
TACTTTT81500.018.76602414-15
CATGGGG31400.018.6068974
Produced by FastQC (version 0.11.5)