FastQCFastQC Report
Thu 26 May 2016
SRR937349_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937349_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1991011
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67870.3408820945740631No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48920.24570431805750947No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34110.17131999772979659No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31270.15705588768721018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39450.048.6987881
GTATCAA70150.040.537531
ATCAACG87900.031.7978063
TCAACGC88750.031.4940574
TATCAAC89100.031.4236932
CAACGCA91250.030.632755
AACGCAG93550.029.8578036
GTACATG83150.025.9643441
ACGCAGA106950.025.8078887
CGCAGAG107650.025.5076798
TACATGG82350.024.994472
ACATGGG82400.024.4594863
AGACGCG1000.001646373523.7690335
GCAGAGT116550.022.6212189
GAGTACT61250.021.56070912-13
AGAGTAC104950.020.43643210-11
AGTACTT65650.020.18802312-13
GTACTTT65600.020.1282114-15
TAGACTG9700.019.6033295
CATGGGA53350.019.2459344
Produced by FastQC (version 0.11.5)