##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937349_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1991011 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.65930826097897 33.0 31.0 34.0 30.0 34.0 2 31.76770394538252 33.0 31.0 34.0 30.0 34.0 3 31.816493731074313 33.0 31.0 34.0 30.0 34.0 4 35.44487951096202 37.0 35.0 37.0 33.0 37.0 5 35.43766709475739 37.0 35.0 37.0 33.0 37.0 6 35.49014545876442 37.0 35.0 37.0 33.0 37.0 7 35.427446659008915 37.0 35.0 37.0 33.0 37.0 8 35.41744872328681 37.0 35.0 37.0 33.0 37.0 9 37.03554927622198 39.0 37.0 39.0 33.0 39.0 10-11 36.939839609123204 39.0 37.0 39.0 33.0 39.0 12-13 36.83374125004834 39.0 37.0 39.0 33.0 39.0 14-15 38.00074961916333 40.0 38.0 41.0 33.0 41.0 16-17 37.9443958370898 40.0 38.0 41.0 33.0 41.0 18-19 37.896709008639334 40.0 38.0 41.0 33.0 41.0 20-21 37.83091128075134 40.0 38.0 41.0 33.0 41.0 22-23 37.69620258250708 40.0 37.0 41.0 32.0 41.0 24-25 37.55864357354128 40.0 37.0 41.0 32.0 41.0 26-27 37.3972838422289 40.0 37.0 41.0 32.0 41.0 28-29 37.21276025094788 39.0 37.0 41.0 31.5 41.0 30-31 37.22247441124132 39.0 37.0 41.0 31.5 41.0 32-33 37.10029803953871 39.0 36.0 41.0 31.0 41.0 34-35 36.91093946743639 39.0 36.0 41.0 30.5 41.0 36-37 36.94337022748744 39.0 36.0 41.0 31.0 41.0 38-39 36.935932799969464 39.5 36.0 41.0 31.0 41.0 40-41 36.788507697847976 39.5 36.0 41.0 30.5 41.0 42-43 36.57899805676614 39.0 36.0 41.0 30.0 41.0 44-45 36.38650313835534 39.0 36.0 41.0 30.0 41.0 46-47 36.10305392586982 39.0 35.5 41.0 29.5 41.0 48-49 35.82984800184429 39.0 35.0 41.0 28.0 41.0 50-51 34.93006894487273 38.0 34.0 39.5 26.5 40.5 52-53 35.07893577684905 38.5 34.5 40.0 27.0 40.5 54-55 35.44936316273491 39.0 35.0 40.0 27.0 41.0 56-57 35.24994713740908 39.0 35.0 40.0 26.5 41.0 58-59 35.028572920993405 38.0 34.5 40.0 26.0 41.0 60-61 34.75979565155592 38.0 34.0 40.0 26.0 41.0 62-63 34.45738798027736 38.0 34.0 40.0 25.5 41.0 64-65 34.113342919752824 37.5 34.0 40.0 24.5 41.0 66-67 33.74255039274017 37.0 33.5 39.5 23.5 41.0 68-69 33.261146723950795 36.0 33.0 39.0 22.0 41.0 70-71 32.83949812431976 36.0 32.0 39.0 21.5 40.0 72-73 32.43603149354775 35.0 32.0 38.5 20.5 40.0 74-75 31.97214103789482 35.0 31.5 37.5 19.5 39.5 76-77 31.531423231714943 35.0 31.0 37.0 18.0 39.0 78-79 31.052639337502406 35.0 31.0 36.5 15.0 39.0 80-81 30.592556495167532 34.5 30.5 36.0 10.0 38.5 82-83 30.14214687914833 34.0 30.0 35.5 7.0 37.0 84-85 29.672403618061377 34.0 29.5 35.0 4.5 37.0 86-87 29.245413259896605 34.0 29.0 35.0 2.0 36.0 88-89 28.86326117736165 34.0 29.0 35.0 2.0 36.0 90-91 28.431558891437568 34.0 29.0 35.0 2.0 35.5 92-93 27.98794958942969 34.0 27.5 35.0 2.0 35.0 94-95 27.461296547331983 33.0 26.5 35.0 2.0 35.0 96-97 26.850154017230444 33.0 25.5 35.0 2.0 35.0 98-99 26.150401730578082 33.0 25.0 35.0 2.0 35.0 100-101 24.333156873568253 30.5 19.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07283966865458069 1101 2 0.021384240575354596 1101 3 0.13985724460873428 1101 4 0.10225685289402264 1101 5 0.07289307151302893 1101 6 0.015564212804321187 1101 7 0.05578263186217214 1101 8 -0.031142125516268493 1101 9 -0.10191380606494249 1101 10-11 0.06994286962599716 1101 12-13 0.09028657740235957 1101 14-15 0.21769067975040457 1101 16-17 0.13095136867042356 1101 18-19 0.2241697829984588 1101 20-21 0.24198898627614085 1101 22-23 0.19386167898757378 1101 24-25 0.11693828012433727 1101 26-27 0.29682636651328664 1101 28-29 0.26220484366335484 1101 30-31 0.1444992084642749 1101 32-33 0.20037811127745186 1101 34-35 0.006836714602755478 1101 36-37 0.029211763955750314 1101 38-39 0.26922103488660554 1101 40-41 0.28289866094613814 1101 42-43 0.37178166212784447 1101 44-45 0.30403784838482295 1101 46-47 0.3527683615401074 1101 48-49 0.3563184451833834 1101 50-51 0.3779291696155198 1101 52-53 0.43837556566307256 1101 54-55 0.4259783479988073 1101 56-57 0.5014944498141105 1101 58-59 0.4941734661249839 1101 60-61 0.5917037045745914 1101 62-63 0.5798569808751282 1101 64-65 0.3645389151097902 1101 66-67 0.5125308432750941 1101 68-69 0.46248227035511746 1101 70-71 0.34995728828988604 1101 72-73 0.40914950877950673 1101 74-75 0.5408503736967738 1101 76-77 0.43938533398157986 1101 78-79 0.5149377516344735 1101 80-81 0.5313764064844513 1101 82-83 0.47064134177211514 1101 84-85 0.5413079900222328 1101 86-87 0.619288827717444 1101 88-89 0.6915201326922116 1101 90-91 0.8155035605831564 1101 92-93 0.7357294250795725 1101 94-95 0.7327233079177375 1101 96-97 0.8355455839606307 1101 98-99 0.8922893994641257 1101 100-101 1.2101412960747702 1102 1 -4.818579981851201E-4 1102 2 -0.17622177811130157 1102 3 -0.06028721040005891 1102 4 -0.2510289544693691 1102 5 -0.1049468228253474 1102 6 -0.041988730228446514 1102 7 -0.03274625301860823 1102 8 -0.028505209508281837 1102 9 0.07941830851426346 1102 10-11 -0.10450580055817937 1102 12-13 0.020269969156991863 1102 14-15 0.07938711475857474 1102 16-17 0.0799635113245003 1102 18-19 -0.08031893196405804 1102 20-21 0.09471982524417655 1102 22-23 0.09167914335329641 1102 24-25 0.059673362968325705 1102 26-27 0.08295732545242629 1102 28-29 0.14083284753181147 1102 30-31 0.056558413254414575 1102 32-33 0.0737417366357036 1102 34-35 0.0662395522867385 1102 36-37 0.0814389827071551 1102 38-39 0.22393391355392112 1102 40-41 0.10450148584408936 1102 42-43 0.07675611045797126 1102 44-45 0.26402047841813214 1102 46-47 0.03189726519187985 1102 48-49 0.09172855730604113 1102 50-51 0.03624043343362615 1102 52-53 0.05845970866537442 1102 54-55 0.25739465998898226 1102 56-57 0.20852962637703598 1102 58-59 0.23685985321272085 1102 60-61 0.23543106546863868 1102 62-63 0.25827136271787055 1102 64-65 0.38079078370741115 1102 66-67 0.294238853727002 1102 68-69 0.2275792470015645 1102 70-71 0.0628575150379973 1102 72-73 0.1729132690712447 1102 74-75 0.12250445155829581 1102 76-77 0.14404652441599097 1102 78-79 0.27689881679293293 1102 80-81 0.22224860460817197 1102 82-83 0.25900462027511395 1102 84-85 0.20905813726568567 1102 86-87 0.14981161338674553 1102 88-89 0.02088380973869164 1102 90-91 0.060641534159586286 1102 92-93 -0.1672899158410459 1102 94-95 -0.20498230583242005 1102 96-97 0.021854459238284107 1102 98-99 0.26567682861384156 1102 100-101 0.38887764119982293 1103 1 0.08987084657768207 1103 2 0.05297596987826125 1103 3 0.06522017299991134 1103 4 0.014534008078136651 1103 5 -0.057202171750752484 1103 6 0.016382328018821113 1103 7 0.09001729389783009 1103 8 0.08624316197729343 1103 9 0.16310939421725834 1103 10-11 0.039019335585358306 1103 12-13 0.0817967665321433 1103 14-15 0.014060717467813788 1103 16-17 0.03597606136843723 1103 18-19 -0.03127821309412582 1103 20-21 0.05832890432894544 1103 22-23 0.11287166460611076 1103 24-25 0.17105259917570237 1103 26-27 -0.028132931237237813 1103 28-29 -0.15564851969919857 1103 30-31 -0.08935420916794357 1103 32-33 -0.15034638876324635 1103 34-35 -0.012668481717767577 1103 36-37 -0.012115265051377833 1103 38-39 -0.14058617212503322 1103 40-41 -0.041243878331528094 1103 42-43 -0.17254647940744405 1103 44-45 -0.1504000535498875 1103 46-47 -0.07574152910651577 1103 48-49 -0.03594270044926162 1103 50-51 -0.1643536838835118 1103 52-53 -0.11598703711010927 1103 54-55 -0.06452378208558684 1103 56-57 0.029990094673415513 1103 58-59 -0.09063306251582759 1103 60-61 -0.02128777508784907 1103 62-63 -0.017818920679694372 1103 64-65 -0.07394506177134019 1103 66-67 0.14611087746499152 1103 68-69 0.11775728188742818 1103 70-71 0.011490270679374248 1103 72-73 0.13763633481809023 1103 74-75 0.19276880638882332 1103 76-77 0.12997226041904852 1103 78-79 0.184649851056502 1103 80-81 0.14573887358346482 1103 82-83 0.05638944911751409 1103 84-85 -0.030750594082181948 1103 86-87 0.23371709678644592 1103 88-89 0.3741253480071123 1103 90-91 0.46729106770605 1103 92-93 0.4657744689023282 1103 94-95 0.40290687506288236 1103 96-97 0.7199634061189322 1103 98-99 0.8569591685466982 1103 100-101 1.0597620867838025 1104 1 0.14429760568549455 1104 2 0.15970873955929932 1104 3 0.10941127228463188 1104 4 0.05523076151687434 1104 5 0.14814791382783454 1104 6 0.11199856163886324 1104 7 0.028482765448039515 1104 8 0.2039499271610481 1104 9 0.08986741704002554 1104 10-11 0.0409593654768301 1104 12-13 0.2413771602207575 1104 14-15 0.2980664055277771 1104 16-17 0.12498531633294618 1104 18-19 0.12676786914268945 1104 20-21 0.23714522095072255 1104 22-23 0.20877128404587353 1104 24-25 0.12765008416893409 1104 26-27 0.2755711567337471 1104 28-29 0.3257654663641034 1104 30-31 0.23225648557826162 1104 32-33 0.1579144536772219 1104 34-35 0.20587094546177553 1104 36-37 0.026081061172547493 1104 38-39 0.09506368262193376 1104 40-41 0.08501199756731381 1104 42-43 0.23098672264210052 1104 44-45 0.1384439438786842 1104 46-47 0.15270060159679844 1104 48-49 0.33194240996203206 1104 50-51 0.26878123190770253 1104 52-53 0.18198423506776606 1104 54-55 0.3260825781256642 1104 56-57 0.3013166576692967 1104 58-59 0.20935466353505205 1104 60-61 0.3623198572741515 1104 62-63 0.3024646957004009 1104 64-65 0.25773515370767086 1104 66-67 0.30472712193493123 1104 68-69 0.3678516760154551 1104 70-71 0.2928751654489048 1104 72-73 0.32265418290322145 1104 74-75 0.2933903700221272 1104 76-77 0.2515830907890013 1104 78-79 0.2356773365651037 1104 80-81 0.28944294799208237 1104 82-83 0.28334256670058977 1104 84-85 0.38927201439647163 1104 86-87 0.45995425660818157 1104 88-89 0.17109158040653938 1104 90-91 0.3598543429652956 1104 92-93 0.23141744752531324 1104 94-95 0.37130972912901683 1104 96-97 0.5620212794843304 1104 98-99 0.5116145214803751 1104 100-101 0.8220473604860885 1105 1 -0.04777119507895833 1105 2 -0.030661276897671286 1105 3 -0.11463360393415911 1105 4 -0.093713691775406 1105 5 -0.028454871470607657 1105 6 -0.0803356741736323 1105 7 -0.1338396142656464 1105 8 -0.11393508637613792 1105 9 0.048105636147809605 1105 10-11 0.024661653526941052 1105 12-13 0.07695784362752534 1105 14-15 0.06778380567995868 1105 16-17 0.016068468285659776 1105 18-19 0.1347486162538729 1105 20-21 0.051128339281667934 1105 22-23 0.057163632438218315 1105 24-25 0.03804805978430181 1105 26-27 0.07463682536463523 1105 28-29 0.10786983542499229 1105 30-31 -0.06147393858694272 1105 32-33 0.1091451875166527 1105 34-35 0.22150506979154727 1105 36-37 0.23289920277330367 1105 38-39 0.18667416553676475 1105 40-41 0.1148203534626262 1105 42-43 -0.05115303775853164 1105 44-45 0.008301139822833647 1105 46-47 0.02944141312021742 1105 48-49 0.13688304098961623 1105 50-51 0.22655936359887363 1105 52-53 0.14233708672497158 1105 54-55 0.11399112690377677 1105 56-57 0.152319117367405 1105 58-59 0.14710129693386165 1105 60-61 0.22819552749763972 1105 62-63 0.29121024966334375 1105 64-65 0.26737929378658976 1105 66-67 0.3959483001126287 1105 68-69 0.5158671066395826 1105 70-71 0.5805890618584613 1105 72-73 0.48661341207983355 1105 74-75 0.30628166552212477 1105 76-77 0.42415126335717446 1105 78-79 0.5564998313006413 1105 80-81 0.41884253391340565 1105 82-83 0.49949517415754485 1105 84-85 0.4902346588373767 1105 86-87 0.4647320874729708 1105 88-89 0.5583347719975578 1105 90-91 0.659999447458226 1105 92-93 0.6248718192405569 1105 94-95 0.6201462388350905 1105 96-97 0.721609678203162 1105 98-99 0.9221186711149585 1105 100-101 1.12917340674257 1106 1 0.04655191942918435 1106 2 0.002400745651314651 1106 3 -0.0753678203454129 1106 4 0.023085327042117854 1106 5 0.11016659179341559 1106 6 -0.05319432659494794 1106 7 0.016568772475793025 1106 8 -0.027528454973712257 1106 9 -0.038406938590163975 1106 10-11 -0.19169791438855555 1106 12-13 -0.24358415836947955 1106 14-15 -0.19497389420978806 1106 16-17 -0.19598310814775033 1106 18-19 -0.17306837596176905 1106 20-21 -0.06381983748315889 1106 22-23 0.008628234870030838 1106 24-25 -0.17239939078089606 1106 26-27 -0.053171913882287924 1106 28-29 0.1278523059961998 1106 30-31 0.31299886923321907 1106 32-33 0.396613271872873 1106 34-35 0.2553447232695589 1106 36-37 0.06979755502068485 1106 38-39 -0.008062521630542108 1106 40-41 0.1311154311538374 1106 42-43 0.052313551410328785 1106 44-45 0.07309481731626022 1106 46-47 0.11172156016067447 1106 48-49 0.14371296308446802 1106 50-51 0.20729641771692542 1106 52-53 0.3095320152739731 1106 54-55 0.4172269684070713 1106 56-57 0.285629379357232 1106 58-59 0.32284802629707343 1106 60-61 0.3187193154562564 1106 62-63 0.5314312005655424 1106 64-65 0.4249189164229392 1106 66-67 0.43550897928643195 1106 68-69 0.3714772084649951 1106 70-71 0.3670698535957655 1106 72-73 0.5770568439866821 1106 74-75 0.6045338914446567 1106 76-77 0.6020266151181453 1106 78-79 0.5913929278001291 1106 80-81 0.5229223621311654 1106 82-83 0.46519691782396677 1106 84-85 0.46842693349727327 1106 86-87 0.4481497304785016 1106 88-89 0.6421715501152434 1106 90-91 0.6798592873348852 1106 92-93 0.6744602319492365 1106 94-95 0.8153345116095707 1106 96-97 0.9265118765872806 1106 98-99 0.8530051926238542 1106 100-101 0.8840536462737774 1107 1 -0.1929389857235222 1107 2 -0.2946587572765722 1107 3 -0.1957147522524707 1107 4 -0.2081987267983365 1107 5 -0.09277287710890647 1107 6 -0.023612853392137367 1107 7 -0.23945373210470677 1107 8 -0.08465493973658056 1107 9 -0.19508904836717278 1107 10-11 -0.1439006996319847 1107 12-13 -0.0034391658648402768 1107 14-15 -0.0690633641745464 1107 16-17 -0.11053328728919354 1107 18-19 -0.002351063021841071 1107 20-21 -0.013447400110699448 1107 22-23 -0.01324196486223883 1107 24-25 -0.13705913855028484 1107 26-27 -0.13708821948999628 1107 28-29 -0.010747895662170492 1107 30-31 -0.05661754803373498 1107 32-33 -0.0970343524037176 1107 34-35 -0.003498736508099398 1107 36-37 -0.020407551144700165 1107 38-39 -0.01657576754202239 1107 40-41 -0.03471721844658049 1107 42-43 -0.14717229514698005 1107 44-45 -0.1231363577515765 1107 46-47 -0.10679450979051097 1107 48-49 -0.0765931504816848 1107 50-51 -0.1749495232400804 1107 52-53 -0.19692163517434125 1107 54-55 -0.1127722844562129 1107 56-57 0.018247312146463912 1107 58-59 -0.05387630013175482 1107 60-61 -0.034345110348617425 1107 62-63 -0.03559416027064799 1107 64-65 -0.14014503916560983 1107 66-67 -0.130029819386273 1107 68-69 -0.13424986866076694 1107 70-71 -0.014962742128552975 1107 72-73 0.1308714224747689 1107 74-75 0.025328488480923284 1107 76-77 -0.08294661476759657 1107 78-79 0.18245501349054294 1107 80-81 0.1320826352953155 1107 82-83 0.2660597921891572 1107 84-85 0.2818944841157851 1107 86-87 0.17616009168825997 1107 88-89 0.25190451385580914 1107 90-91 0.24414354749474398 1107 92-93 0.26239342558857004 1107 94-95 0.24742989423610595 1107 96-97 0.26411982584778215 1107 98-99 0.378222085042335 1107 100-101 0.6816752153568473 1108 1 -0.019560097080983496 1108 2 -0.14354444703115732 1108 3 -0.02552381570193063 1108 4 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72-73 -1.0870949395189768 2316 74-75 -1.3795976080853514 2316 76-77 -0.9983172230058379 2316 78-79 -0.9023239870566471 2316 80-81 -0.9735279421284702 2316 82-83 -1.244074343245643 2316 84-85 -1.4744192545994963 2316 86-87 -1.6464923315607791 2316 88-89 -1.488610619775418 2316 90-91 -1.1580420632810515 2316 92-93 -1.230885337733639 2316 94-95 -1.3175255289583774 2316 96-97 -1.4886417374235066 2316 98-99 -1.565166281180133 2316 100-101 -1.6830937341249772 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7516.0 3 1288.0 4 1165.0 5 1364.0 6 1625.0 7 1970.0 8 2351.0 9 2877.0 10 3873.0 11 4718.0 12 6134.0 13 8153.0 14 9149.0 15 11061.0 16 13715.0 17 17818.0 18 15499.0 19 10885.0 20 10878.0 21 11889.0 22 13069.0 23 14478.0 24 16529.0 25 19114.0 26 21706.0 27 25907.0 28 31002.0 29 38149.0 30 46756.0 31 57737.0 32 73878.0 33 96992.0 34 130840.0 35 185271.0 36 275381.0 37 400741.0 38 350813.0 39 48710.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.48998809558609 19.536594732826043 14.909950819953046 23.063466351634816 2 14.697154879084628 20.22608217267809 41.25354796261049 23.823214985626784 3 18.74298767140058 24.8169552374758 30.24242567750367 26.19763141361995 4 12.08521923478415 16.546543308895544 38.36900928206099 32.99922817425931 5 13.16395937321963 37.712706019011755 35.02611584868575 14.09721875908287 6 31.376063755352124 36.70110752373157 18.00539013004167 13.917438590874633 7 27.813637393339878 32.8595232701553 21.376123234916562 17.95071610158826 8 25.91256510950882 35.25727411713595 20.27676355085753 18.553397222497697 9 25.951971649127753 16.67345402925194 20.781662543071228 36.59291177854908 10-11 24.086603654516743 27.078216606300092 28.23731024426123 20.597869494921934 12-13 25.150073954592184 24.3201111181592 28.638399801848006 21.89141512540061 14-15 22.16399598327876 25.67014564389602 26.873047951497337 25.292810421327882 16-17 21.435194857170185 29.112942402420394 27.400947919786756 22.050914820622666 18-19 21.313411229500915 27.694153810997058 29.85209929307554 21.140335666426488 20-21 22.068959615753425 26.776805673496117 29.906133015476875 21.24810169527358 22-23 21.691588592172533 26.863708183186848 29.784423289009602 21.660279935631014 24-25 21.518738233475528 26.857157381330644 29.957643657608955 21.666460727584873 26-27 21.478639629191132 27.135986743019146 29.687638429136364 21.697735198653362 28-29 21.34054856754529 27.219345217495267 29.78916833938448 21.650937875574964 30-31 21.482559200548742 26.949663014681356 29.853412959618396 21.714364825151502 32-33 21.184411569994136 27.070977096123784 29.789034633963386 21.955576699918694 34-35 21.66800648954158 27.030486046126484 29.65466799491186 21.646839469420073 36-37 21.643639376452338 26.90585149128099 29.55647518743099 21.894033944835677 38-39 21.430122671890253 27.152923507670675 29.666430199425182 21.750523621013894 40-41 21.6745991621493 27.002830331836087 29.606574563654032 21.715995942360582 42-43 21.434003516081315 27.03474458080067 29.49698294453943 22.03426895857859 44-45 21.526693386733196 27.198339610636076 29.349813392451736 21.925153610178995 46-47 21.60015664123344 27.350908325365786 28.99717648136576 22.051758552035015 48-49 21.678037421049478 27.48776886720506 28.736731253356986 22.097462458388474 50-51 21.743204941677533 27.49573342327965 28.696592276363415 22.0644693586794 52-53 21.842362706551285 27.56991111856279 28.483874914469304 22.10385126041662 54-55 21.718898758502913 27.46442886049004 28.573690572748085 22.242981808258957 56-57 21.72659571389568 27.761162857117693 28.44457920913537 22.067662219851258 58-59 21.88875475716147 27.54793392131002 28.494057416542418 22.069253904986095 60-61 21.744817189701337 27.46247182099706 28.811566356252044 21.981144633049556 62-63 21.668051882824553 27.439861362634993 28.95413789606677 21.937948858473685 64-65 21.669348113920158 27.50196139017901 28.727886866913828 22.100803628987006 66-67 21.56983686591297 27.679016539896445 28.500688900213277 22.250457693977314 68-69 21.59145179999849 27.56656106809724 28.664350040385788 22.17763709151848 70-71 21.523244551509737 27.431774184497392 28.741973562991912 22.303007701000958 72-73 21.599816378041773 27.36928575437392 28.626577718069324 22.404320149514984 74-75 21.51385920530105 27.666395645339026 28.444682332554994 22.375062816804935 76-77 21.586802234861832 27.551593094075606 28.469849499169474 22.391755171893088 78-79 21.527161719535474 27.509321092282274 28.504057490660035 22.45945969752222 80-81 21.492992577889883 27.694037752713086 28.386073528612464 22.426896140784567 82-83 21.583941633394193 27.548172205647152 28.512937570230818 22.354948590727833 84-85 21.57926599742745 27.498953265309 28.358131216245326 22.56364952101823 86-87 21.58104769906652 27.448119190923343 28.46468637497009 22.50614673504005 88-89 21.752681353082988 27.198558536794053 28.437190153356788 22.61156995676617 90-91 21.563084910584017 27.11227112522767 28.655994693371596 22.668649270816715 92-93 21.533699781007165 27.025532722341143 28.68297779923023 22.757789697421458 94-95 21.313263393549192 27.168606418595846 28.61025884162484 22.907871346230117 96-97 21.310946962509934 27.070821504081877 28.704830964798866 22.913400568609323 98-99 21.193874271724816 27.095272021524053 28.6199092460466 23.090944460704534 100-101 21.18086824062423 26.9741965466736 28.90018979380839 22.94474541889378 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2783.0 1 1575.5 2 351.5 3 324.5 4 276.5 5 269.0 6 421.0 7 824.0 8 1376.5 9 1736.0 10 1731.5 11 1634.5 12 1739.5 13 1890.0 14 2060.0 15 2222.5 16 2330.0 17 2482.0 18 2647.0 19 2876.5 20 3248.5 21 3791.5 22 4548.5 23 5572.5 24 6741.0 25 8389.0 26 10962.5 27 14280.0 28 17375.0 29 20733.5 30 25167.5 31 29900.5 32 34525.5 33 38728.5 34 43953.5 35 48915.5 36 53819.0 37 58841.0 38 61861.5 39 64360.5 40 66859.0 41 69616.5 42 72026.0 43 75479.0 44 78977.0 45 80296.0 46 79936.0 47 79527.0 48 80438.5 49 78739.5 50 74323.5 51 69300.0 52 64991.0 53 62033.5 54 58590.0 55 52920.0 56 46391.5 57 40701.0 58 36245.5 59 31877.0 60 26304.5 61 20851.0 62 15913.0 63 11830.0 64 8789.0 65 6589.5 66 4691.5 67 3315.5 68 2302.5 69 1508.5 70 1069.0 71 749.0 72 528.0 73 405.5 74 315.5 75 223.0 76 143.0 77 87.5 78 61.5 79 43.0 80 27.0 81 19.5 82 13.5 83 9.0 84 9.0 85 11.5 86 11.0 87 10.5 88 8.0 89 6.0 90 7.0 91 4.5 92 2.5 93 1.5 94 2.5 95 3.5 96 2.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3452015081785083 2 0.30482001355090454 3 0.3070801718323003 4 0.3067285916551943 5 0.29221335291467504 6 0.22184709175388784 7 0.22907959825435417 8 0.2285271151189019 9 0.2248104103894956 10-11 0.2347802196974301 12-13 0.2339012692546651 14-15 0.21677429205564408 16-17 0.22882846955642133 18-19 0.21629714752957166 20-21 0.219486481993319 22-23 0.2179797098057218 24-25 0.2098180271229039 26-27 0.22275115506644613 28-29 0.22468484604052916 30-31 0.23347435046817921 32-33 0.23435330091094425 34-35 0.22250002636851327 36-37 0.21830617711303454 38-39 0.20720628866440216 40-41 0.20800990049778728 42-43 0.20793456188840745 44-45 0.22546334500412102 46-47 0.21624692178998506 48-49 0.22511176482701503 50-51 0.23309765742127994 52-53 0.24520206066164377 54-55 0.24007903522381344 56-57 0.20132987713277323 58-59 0.18804516901212498 60-61 0.19776384962212668 62-63 0.19871813867427152 64-65 0.1958803843876302 66-67 0.2083614806748933 68-69 0.19718625361688108 70-71 0.20399184133086157 72-73 0.21700030788378366 74-75 0.20504658186217958 76-77 0.21652316335771124 78-79 0.21363518333148337 80-81 0.19964731485662307 82-83 0.21388631202941621 84-85 0.1948256438563122 86-87 0.19992355642434925 88-89 0.20798478762799402 90-91 0.20401695420065483 92-93 0.21034539738856287 94-95 0.20888885094055232 96-97 0.20381605124230856 98-99 0.21147547652926074 100-101 0.19359511323644119 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1991011.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.29150779932017 #Duplication Level Percentage of deduplicated Percentage of total 1 77.63761795089935 46.03251430256389 2 12.75709570389703 15.127748788485693 3 4.246255456737638 7.5530066559319655 4 1.8115185756952659 4.296306710377969 5 0.9816261960528825 2.9101048629643236 6 0.5668878338679737 2.0166980653873607 7 0.38712785954310913 1.6067376152404067 8 0.2711985643695039 1.2863817435583098 9 0.19048204781531655 1.0164571041305086 >10 1.0463676199241803 11.153276528960824 >50 0.07353196828762697 2.9874041824249122 >100 0.029360030337635592 2.855310932239099 >500 5.073777668999374E-4 0.18034874097597817 >1k 3.382518445999471E-4 0.6374127172720448 >5k 8.456296114998677E-5 0.3402910494867748 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6787 0.3408820945740631 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4892 0.24570431805750947 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3411 0.17131999772979659 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3127 0.15705588768721018 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.022573958657185E-5 0.0 10-11 0.0 0.0 0.0 1.5067721875971555E-4 0.0 12-13 0.0 0.0 0.0 1.5067721875971555E-4 1.7579008855300146E-4 14-15 0.0 0.0 0.0 1.5067721875971555E-4 2.260158281395733E-4 16-17 0.0 0.0 0.0 1.5067721875971555E-4 2.511286979328592E-4 18-19 0.0 0.0 0.0 1.5067721875971555E-4 3.7669304689928886E-4 20-21 0.0 0.0 0.0 1.5067721875971555E-4 4.520316562791466E-4 22-23 0.0 0.0 0.0 1.5067721875971555E-4 4.520316562791466E-4 24-25 0.0 0.0 0.0 1.7579008855300146E-4 4.7714452607243256E-4 26-27 0.0 0.0 0.0 3.2646730731271704E-4 5.022573958657184E-4 28-29 0.0 0.0 0.0 7.533860937985777E-4 5.022573958657184E-4 30-31 0.0 0.0 0.0 0.0020341424532561597 5.022573958657184E-4 32-33 0.0 0.0 0.0 0.0034153502918868855 5.524831354522903E-4 34-35 2.5112869793285925E-5 0.0 0.0 0.005399267005556474 5.524831354522903E-4 36-37 5.022573958657185E-5 0.0 0.0 0.007408296589019347 5.524831354522903E-4 38-39 5.022573958657185E-5 0.0 0.0 0.009869357828761367 5.524831354522903E-4 40-41 7.533860937985778E-5 0.0 0.0 0.013134030901888537 5.524831354522903E-4 42-43 1.004514791731437E-4 0.0 0.0 0.017880363292819577 5.524831354522903E-4 44-45 1.5067721875971555E-4 0.0 0.0 0.026142497454810648 5.524831354522903E-4 46-47 2.511286979328592E-4 0.0 0.0 0.04520316562791466 5.524831354522903E-4 48-49 2.511286979328592E-4 0.0 0.0 0.08334961484391598 5.524831354522903E-4 50-51 2.511286979328592E-4 0.0 0.0 0.12046643639839258 5.524831354522903E-4 52-53 2.511286979328592E-4 0.0 0.0 0.15888912718212006 6.027088750388622E-4 54-55 2.511286979328592E-4 0.0 0.0 0.20954178555517775 6.027088750388622E-4 56-57 2.511286979328592E-4 0.0 0.0 0.2476128961617992 6.278217448321481E-4 58-59 2.511286979328592E-4 0.0 0.0 0.2952268972898693 6.52934614625434E-4 60-61 2.511286979328592E-4 0.0 0.0 0.3697367819665487 6.7804748441872E-4 62-63 2.511286979328592E-4 0.0 0.0 0.4237043391523201 7.031603542120058E-4 64-65 2.511286979328592E-4 0.0 0.0 0.48882201052631047 7.031603542120058E-4 66-67 2.511286979328592E-4 0.0 0.0 0.6140850050552207 7.031603542120058E-4 68-69 2.511286979328592E-4 0.0 0.0 0.7488908901055795 7.031603542120058E-4 70-71 2.7624156772614516E-4 0.0 0.0 0.8714416946968149 7.031603542120058E-4 72-73 3.013544375194311E-4 0.0 0.0 1.0165187434926277 7.031603542120058E-4 74-75 3.013544375194311E-4 0.0 0.0 1.1598127785331172 7.031603542120058E-4 76-77 3.013544375194311E-4 0.0 0.0 1.285301788890167 7.031603542120058E-4 78-79 3.013544375194311E-4 0.0 0.0 1.4479578465412797 7.031603542120058E-4 80-81 4.018059166925748E-4 0.0 0.0 1.670633662998346 7.031603542120058E-4 82-83 4.018059166925748E-4 0.0 0.0 1.878869579324273 7.282732240052917E-4 84-85 4.7714452607243256E-4 0.0 0.0 2.1262564596579328 7.533860937985777E-4 86-87 5.775960052455762E-4 0.0 0.0 2.558172707232657 7.533860937985777E-4 88-89 6.027088750388622E-4 0.0 0.0 3.114950143419599 7.533860937985777E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3945 0.0 48.698788 1 GTATCAA 7015 0.0 40.53753 1 ATCAACG 8790 0.0 31.797806 3 TCAACGC 8875 0.0 31.494057 4 TATCAAC 8910 0.0 31.423693 2 CAACGCA 9125 0.0 30.63275 5 AACGCAG 9355 0.0 29.857803 6 GTACATG 8315 0.0 25.964344 1 ACGCAGA 10695 0.0 25.807888 7 CGCAGAG 10765 0.0 25.507679 8 TACATGG 8235 0.0 24.99447 2 ACATGGG 8240 0.0 24.459486 3 AGACGCG 100 0.0016463735 23.769033 5 GCAGAGT 11655 0.0 22.621218 9 GAGTACT 6125 0.0 21.560709 12-13 AGAGTAC 10495 0.0 20.436432 10-11 AGTACTT 6565 0.0 20.188023 12-13 GTACTTT 6560 0.0 20.12821 14-15 TAGACTG 970 0.0 19.603329 5 CATGGGA 5335 0.0 19.245934 4 >>END_MODULE