FastQCFastQC Report
Thu 26 May 2016
SRR937349_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937349_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1991011
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76910.38628616316032405No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60910.3059249798218091No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42860.21526751986804693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48400.046.3513451
GTATCAA81650.037.7793271
ATCAACG101750.029.9742343
TCAACGC103050.029.64224
TATCAAC104900.029.2552952
CAACGCA105800.028.8717255
AACGCAG107700.028.3566866
CGCGTGA350.00839304727.14349626-27
GTACATG81600.024.6385941
TATACCG1351.3420808E-524.6326855
ACGCAGA125200.024.3569897
CGCAGAG126100.023.9571388
TACATGG82550.023.7097842
ACATGGG81550.022.7771953
GAGTACT77650.022.35830312-13
GCAGAGT137200.021.915059
GTACTTT82050.020.83934814-15
AGAGTAC121350.020.51088710-11
AGTACTT81200.020.41560712-13
CAGAGTA131500.019.09027310-11
Produced by FastQC (version 0.11.5)