##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937349_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1991011 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.174307926977804 33.0 31.0 34.0 31.0 34.0 2 32.40147794261308 34.0 31.0 34.0 31.0 34.0 3 32.48601137813905 34.0 31.0 34.0 31.0 34.0 4 36.0978809258211 37.0 35.0 37.0 35.0 37.0 5 35.9871587851599 37.0 35.0 37.0 35.0 37.0 6 36.06297956163979 37.0 35.0 37.0 35.0 37.0 7 36.01483567895908 37.0 35.0 37.0 35.0 37.0 8 35.99354649472052 37.0 35.0 37.0 35.0 37.0 9 37.695782695324134 39.0 37.0 39.0 35.0 39.0 10-11 37.619386834125976 39.0 37.0 39.0 35.0 39.0 12-13 37.541491734601166 39.0 37.0 39.0 35.0 39.0 14-15 38.77106329397477 40.0 38.0 41.0 35.0 41.0 16-17 38.744813062308545 40.0 38.0 41.0 35.0 41.0 18-19 38.740405502531125 40.0 38.0 41.0 35.0 41.0 20-21 38.62959019312299 40.0 38.0 41.0 34.0 41.0 22-23 38.505469080783584 40.0 38.0 41.0 34.0 41.0 24-25 38.3777249347191 40.0 38.0 41.0 34.0 41.0 26-27 38.34154934352447 40.0 38.0 41.0 34.0 41.0 28-29 38.247928313806405 40.0 38.0 41.0 34.0 41.0 30-31 38.35225119298688 40.0 38.0 41.0 34.0 41.0 32-33 38.308125369473096 40.0 38.0 41.0 34.0 41.0 34-35 38.21373940174112 40.0 38.0 41.0 34.0 41.0 36-37 38.07334314074608 40.0 38.0 41.0 33.5 41.0 38-39 37.962701863525616 40.0 37.0 41.0 33.0 41.0 40-41 37.81362508795783 40.0 37.0 41.0 33.0 41.0 42-43 37.62678508556708 40.0 37.0 41.0 33.0 41.0 44-45 37.36480511659654 40.0 37.0 41.0 32.5 41.0 46-47 37.05335379864802 40.0 37.0 41.0 31.5 41.0 48-49 36.787424328645095 40.0 36.5 41.0 31.0 41.0 50-51 36.59172852385045 39.5 36.0 41.0 31.0 41.0 52-53 36.375453475646296 39.0 36.0 41.0 30.0 41.0 54-55 36.162912208923004 39.0 35.5 40.5 30.0 41.0 56-57 35.93212794906708 39.0 35.0 40.0 29.0 41.0 58-59 35.70184544434963 38.5 35.0 40.0 29.0 41.0 60-61 35.39286648843226 38.0 35.0 40.0 28.0 41.0 62-63 35.03600607932352 38.0 34.0 40.0 28.0 41.0 64-65 34.68773326716929 37.5 34.0 40.0 27.0 41.0 66-67 34.27794874061469 37.0 34.0 39.5 26.0 41.0 68-69 33.87955290051135 36.0 33.0 39.0 26.0 40.0 70-71 33.482872269414884 36.0 33.0 39.0 26.0 40.0 72-73 33.03545535408895 35.0 32.5 38.0 25.0 40.0 74-75 32.57776928404715 35.0 32.0 37.0 24.5 39.0 76-77 31.216565604107664 34.0 30.5 36.0 22.5 38.5 78-79 31.86862151941903 35.0 31.5 36.0 24.0 39.0 80-81 31.72620944836568 35.0 32.0 36.0 24.0 38.0 82-83 31.39340214594495 34.5 31.5 36.0 22.5 37.0 84-85 31.020687479878312 34.0 31.0 35.0 21.0 37.0 86-87 30.65054663183679 34.0 31.0 35.0 20.0 36.0 88-89 30.258487773297084 34.0 31.0 35.0 18.0 36.0 90-91 29.82446129127363 34.0 30.0 35.0 15.0 35.5 92-93 29.457950257432028 34.0 30.0 35.0 7.5 35.0 94-95 29.04719863426169 34.0 29.0 35.0 2.0 35.0 96-97 28.580936770314175 34.0 29.0 35.0 2.0 35.0 98-99 28.087100724204937 34.0 29.0 35.0 2.0 35.0 100-101 26.18300802958899 31.5 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14724913357149916 1101 2 0.1730017338454033 1101 3 0.08536542457682117 1101 4 0.06031445648283551 1101 5 0.020755139453612514 1101 6 0.05330051491078791 1101 7 0.025274104532158503 1101 8 0.0809371317534513 1101 9 0.12128694641837967 1101 10-11 0.07267525645707451 1101 12-13 0.04185504898122616 1101 14-15 0.02738337863995355 1101 16-17 0.0515850687726882 1101 18-19 0.012062609970463711 1101 20-21 0.05689293669846762 1101 22-23 -0.09660136455352841 1101 24-25 0.07250106939249434 1101 26-27 -0.04882805058316819 1101 28-29 -0.0032812815888547675 1101 30-31 0.11227771996632185 1101 32-33 0.12865375445516491 1101 34-35 0.09295120241904442 1101 36-37 -0.019612548171934918 1101 38-39 0.05465718950509313 1101 40-41 -0.04307487339697502 1101 42-43 0.15042612016521417 1101 44-45 0.057241811245049234 1101 46-47 0.09750951608732805 1101 48-49 0.182882102283628 1101 50-51 0.19545074334273949 1101 52-53 0.18921069574685845 1101 54-55 0.2068691315720912 1101 56-57 0.20233998259634234 1101 58-59 0.08602943575092326 1101 60-61 0.13764073449106462 1101 62-63 0.0959284591266325 1101 64-65 0.16900718694126482 1101 66-67 0.09656694619997808 1101 68-69 0.017088733300290926 1101 70-71 -0.10686695659727974 1101 72-73 -0.2019912577418168 1101 74-75 -0.09525130001093629 1101 76-77 -0.060062895645014436 1101 78-79 -0.05696890546902367 1101 80-81 0.11455048780141297 1101 82-83 0.03776868611969064 1101 84-85 0.04435103894211423 1101 86-87 0.17989147063091337 1101 88-89 0.10172644623365201 1101 90-91 -0.027978075142243597 1101 92-93 -0.1341789199664163 1101 94-95 -0.13044586728526042 1101 96-97 -0.08274836457961499 1101 98-99 -0.0671286217762237 1101 100-101 0.46492833730004435 1102 1 0.042261219096481284 1102 2 0.006997356668207999 1102 3 0.07600060494834793 1102 4 0.06929433479785274 1102 5 -0.01204118034419821 1102 6 0.034199128441947835 1102 7 -0.02358011696824036 1102 8 0.05017052724868876 1102 9 0.031425321258723216 1102 10-11 0.06105404142994075 1102 12-13 -0.041920014710918 1102 14-15 0.015513311975730915 1102 16-17 0.05078716568267794 1102 18-19 0.013349578958887776 1102 20-21 -0.040622423548121844 1102 22-23 -0.07003578918868669 1102 24-25 -0.061860655743082305 1102 26-27 -0.01685044519017964 1102 28-29 0.027871896489344294 1102 30-31 0.0890805171158533 1102 32-33 -0.04337373008257117 1102 34-35 -0.013428825791841348 1102 36-37 -0.0083775666640733 1102 38-39 -0.09193641992501966 1102 40-41 0.022471450821548444 1102 42-43 0.05969130392367816 1102 44-45 0.03758103567671611 1102 46-47 0.15051477876556874 1102 48-49 0.25593008936701267 1102 50-51 0.08677909012180862 1102 52-53 0.02452137603201976 1102 54-55 0.1143818802026928 1102 56-57 0.05761361057980707 1102 58-59 0.042546505789843536 1102 60-61 0.09522380632274263 1102 62-63 0.0332903461279912 1102 64-65 0.08340861680100886 1102 66-67 0.010590779916206827 1102 68-69 0.10475959351490616 1102 70-71 -0.012427318251674535 1102 72-73 -0.036860479711492644 1102 74-75 -0.00810160680779859 1102 76-77 0.08210715320036854 1102 78-79 0.09632669545429096 1102 80-81 0.11273187813326402 1102 82-83 0.2268726706023365 1102 84-85 0.2200913708621819 1102 86-87 6.485808234231172E-4 1102 88-89 0.09487625913585163 1102 90-91 0.17116430825658213 1102 92-93 0.16129319083139038 1102 94-95 0.1595787798405297 1102 96-97 0.022408352782328222 1102 98-99 -0.05440896378909699 1102 100-101 0.42496258755542726 1103 1 0.034096880023618326 1103 2 0.06915265778646784 1103 3 0.03447675879655776 1103 4 0.06341325440973122 1103 5 -0.027628981242123984 1103 6 -0.0196514835696604 1103 7 0.04251286392759113 1103 8 -0.02365679547092725 1103 9 -0.02040029749662864 1103 10-11 -0.05251604411547106 1103 12-13 -0.06669920711733113 1103 14-15 -0.08443587623752791 1103 16-17 -0.0678176935533088 1103 18-19 -0.05056797641327648 1103 20-21 -0.06049777776168952 1103 22-23 -0.04410021132312636 1103 24-25 -0.01700860417546579 1103 26-27 0.017049896938061693 1103 28-29 -0.19034135810908737 1103 30-31 -0.19266963845769425 1103 32-33 -0.17868418275482867 1103 34-35 0.03940060952294999 1103 36-37 -0.06943210153257695 1103 38-39 0.04477332659027411 1103 40-41 0.06308202020734655 1103 42-43 0.09889910743897445 1103 44-45 -0.028844124771019608 1103 46-47 -0.015165164400016806 1103 48-49 0.08409163746394199 1103 50-51 0.1273979389577491 1103 52-53 0.1665422562711072 1103 54-55 0.10196858073498305 1103 56-57 0.0236788152558276 1103 58-59 0.054846764055270114 1103 60-61 -0.029209318421862918 1103 62-63 -0.09240966802719441 1103 64-65 -0.10467338180483665 1103 66-67 0.0825443428840984 1103 68-69 0.07509011717677083 1103 70-71 -0.01833210190430634 1103 72-73 0.16022755755138007 1103 74-75 0.10323796816261677 1103 76-77 0.07518186054851128 1103 78-79 0.15357976085393332 1103 80-81 0.14988818079791244 1103 82-83 0.011861025956338977 1103 84-85 0.14420179736069372 1103 86-87 0.1955930417377445 1103 88-89 0.09440953851921918 1103 90-91 0.08856671089959889 1103 92-93 0.12728147674484092 1103 94-95 0.2273275839941462 1103 96-97 0.2991364209195311 1103 98-99 0.420268605043848 1103 100-101 0.7424803683422923 1104 1 0.08024774245502897 1104 2 0.11408277086954399 1104 3 0.07104037854011835 1104 4 0.009719812902901026 1104 5 -0.020490289685781704 1104 6 0.02613672096180153 1104 7 0.002964436756187183 1104 8 0.03817542710391564 1104 9 0.0507538877165814 1104 10-11 -0.017321207040282616 1104 12-13 0.03123863380686487 1104 14-15 0.0733597089382485 1104 16-17 -0.010764716205187597 1104 18-19 -0.07888207829925875 1104 20-21 -0.12729993255178584 1104 22-23 -0.0476949865538856 1104 24-25 -0.12554245721899093 1104 26-27 0.0036543394295236453 1104 28-29 -0.01581970306537528 1104 30-31 -0.03486130464967374 1104 32-33 -0.021818976484674124 1104 34-35 0.018523639248961388 1104 36-37 -0.028913964568872075 1104 38-39 -0.13337389027481095 1104 40-41 -0.06100597790477025 1104 42-43 -0.03584057634069637 1104 44-45 0.06026020244026142 1104 46-47 -0.08330972303965467 1104 48-49 -0.0014002156175649816 1104 50-51 -0.20113390685432364 1104 52-53 -0.20023643561072646 1104 54-55 -0.11190030365120407 1104 56-57 -0.12284419873783037 1104 58-59 -0.06808755065149796 1104 60-61 0.04029276611512955 1104 62-63 -0.11219154435460865 1104 64-65 -0.14157538279760473 1104 66-67 0.12061806524058483 1104 68-69 0.0764304188582301 1104 70-71 0.07994527307094046 1104 72-73 0.05599446915452688 1104 74-75 -0.07347604475418734 1104 76-77 0.036344169624182854 1104 78-79 0.021358135376200238 1104 80-81 -0.019133456614714106 1104 82-83 0.10304934766671536 1104 84-85 0.034890548998351534 1104 86-87 -0.017332443863082858 1104 88-89 0.05127644008621601 1104 90-91 0.019512320352031765 1104 92-93 -0.07629410772570111 1104 94-95 -0.04287025036585845 1104 96-97 0.06424802570108312 1104 98-99 0.07558017347823309 1104 100-101 0.44094463284115193 1105 1 -0.03139423875217062 1105 2 0.08035482409812289 1105 3 0.03809104124295715 1105 4 0.04810172085002762 1105 5 -0.009766713744816968 1105 6 0.00599904865021017 1105 7 -0.023472894028969904 1105 8 -0.005055094387472536 1105 9 -0.09424443119478099 1105 10-11 -0.10821501532539202 1105 12-13 -0.017353675655009226 1105 14-15 -0.020560058894567135 1105 16-17 0.034898959893268966 1105 18-19 0.052800249035243496 1105 20-21 -0.03835264795232263 1105 22-23 0.0070023290129910265 1105 24-25 -0.042930222432843834 1105 26-27 -0.06576577346884704 1105 28-29 -0.05349378421772144 1105 30-31 0.10947342664157844 1105 32-33 0.1123142011267646 1105 34-35 0.05196845530246463 1105 36-37 -0.02957755218898228 1105 38-39 -0.07983564108438657 1105 40-41 0.0016366934335536598 1105 42-43 -0.19317277715115466 1105 44-45 -0.053071715239475736 1105 46-47 -0.10366859122460426 1105 48-49 -0.061698210698168054 1105 50-51 -0.046199469311190455 1105 52-53 -0.15327212777685872 1105 54-55 -0.0910990437195025 1105 56-57 0.013270893899004932 1105 58-59 0.058480728090692935 1105 60-61 -0.08337342276845305 1105 62-63 -0.08666554056774345 1105 64-65 0.04414067248367104 1105 66-67 0.11177907814073507 1105 68-69 0.08855195271889471 1105 70-71 0.03646701220137771 1105 72-73 0.03434381706909306 1105 74-75 -0.04606545212620006 1105 76-77 0.003014375424822191 1105 78-79 -0.1167123287812224 1105 80-81 -0.1120534190998903 1105 82-83 0.07310827424738164 1105 84-85 0.02145953556733815 1105 86-87 0.13104574799336888 1105 88-89 0.14194020466302604 1105 90-91 0.17872911000360503 1105 92-93 0.09701728297699574 1105 94-95 0.04713877442577541 1105 96-97 0.08140397763963847 1105 98-99 0.10591055163469676 1105 100-101 0.45591516955082056 1106 1 -0.21741362073454695 1106 2 -0.06211602779241332 1106 3 -0.04070940635480014 1106 4 -0.0015619133384205952 1106 5 -0.1460521179023857 1106 6 -0.15044040635947198 1106 7 -0.142085614828396 1106 8 -0.06776550486912214 1106 9 -0.2071437425802145 1106 10-11 -0.2924169299730579 1106 12-13 -0.3612327352615736 1106 14-15 -0.2616658677043304 1106 16-17 -0.15956687860116858 1106 18-19 -0.14292783840857481 1106 20-21 -0.11642099115943694 1106 22-23 -0.080370799991762 1106 24-25 -0.02978631715269131 1106 26-27 -0.07712695535147418 1106 28-29 -0.061667167075711404 1106 30-31 -0.054144502325378596 1106 32-33 -0.11222046117072182 1106 34-35 -0.2038164102384954 1106 36-37 -0.05119589739857844 1106 38-39 -0.07280692547703893 1106 40-41 -0.12095044282052925 1106 42-43 -0.4080144800079992 1106 44-45 -0.23195797865055567 1106 46-47 -0.11385019076701752 1106 48-49 -0.10182780044928563 1106 50-51 -0.2064417594946093 1106 52-53 -0.057342747995498655 1106 54-55 -0.1769543932418003 1106 56-57 -0.0011767647875160492 1106 58-59 -0.12280620445780244 1106 60-61 -0.028643763118687104 1106 62-63 -0.020391993848441814 1106 64-65 -0.001244750740269751 1106 66-67 -0.13960931012739053 1106 68-69 -0.06756410983114591 1106 70-71 -0.1956194930138011 1106 72-73 -0.13166006192889768 1106 74-75 -0.3060713637300978 1106 76-77 -0.19509264480162614 1106 78-79 -0.06758996405751105 1106 80-81 -0.16594982329066355 1106 82-83 -0.2686321505460292 1106 84-85 -0.3393951036724978 1106 86-87 -0.15269018713028615 1106 88-89 -0.19077920501181111 1106 90-91 -0.11589066295931261 1106 92-93 -0.2542904753955213 1106 94-95 -0.6679931173251248 1106 96-97 -0.15108028575294696 1106 98-99 -0.11681862399607468 1106 100-101 0.2727611354716366 1107 1 -0.07362476020029618 1107 2 -0.0853440971020234 1107 3 -0.06194796710901329 1107 4 -0.0409716616218887 1107 5 -0.1504012761397675 1107 6 -0.14756598633291418 1107 7 -0.16855858801441315 1107 8 -0.10694699945786112 1107 9 -0.07844704375924749 1107 10-11 -0.1351103784065728 1107 12-13 -0.16628248839800364 1107 14-15 -0.30634275849144643 1107 16-17 -0.2783448233994079 1107 18-19 -0.2606395711282943 1107 20-21 -0.26402367619058964 1107 22-23 -0.1472398790189473 1107 24-25 -0.18720116927102737 1107 26-27 -0.30873282633810106 1107 28-29 -0.19588496633995334 1107 30-31 -0.30583018670603934 1107 32-33 -0.3604986194855684 1107 34-35 -0.35095299162875193 1107 36-37 -0.5144754985783706 1107 38-39 -0.39867368975129125 1107 40-41 -0.36271051871317184 1107 42-43 -0.3351042179823551 1107 44-45 -0.3866896503244064 1107 46-47 -0.5058078808944657 1107 48-49 -0.5429625091996257 1107 50-51 -0.6727671423336332 1107 52-53 -0.6944378804340516 1107 54-55 -0.715780483804366 1107 56-57 -0.6775829412091099 1107 58-59 -0.749341690367423 1107 60-61 -0.5040225237354008 1107 62-63 -0.6505999309080011 1107 64-65 -0.7089324791224882 1107 66-67 -0.5950746494953947 1107 68-69 -0.5733677856067132 1107 70-71 -0.7134128281430137 1107 72-73 -0.6351793157792187 1107 74-75 -0.7502806428939621 1107 76-77 -0.7348034754737114 1107 78-79 -0.5205482827394228 1107 80-81 -0.5018045716872308 1107 82-83 -0.3944568284032961 1107 84-85 -0.4914557615777859 1107 86-87 -0.41620684999272584 1107 88-89 -0.40414637198589887 1107 90-91 -0.3488869622334967 1107 92-93 -0.43705382566623996 1107 94-95 -0.44897581459835223 1107 96-97 -0.507108410994995 1107 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-0.40586386899114757 2316 62-63 -0.21666419490716038 2316 64-65 -0.4550368647263241 2316 66-67 -0.36747009279201137 2316 68-69 -0.2386544972056086 2316 70-71 -0.29272284234344426 2316 72-73 -0.2818727963077592 2316 74-75 -0.29367834933341896 2316 76-77 0.02043696485552715 2316 78-79 -0.012674810443499496 2316 80-81 -0.10054637006748735 2316 82-83 -0.33507453910784335 2316 84-85 -0.6521641137155392 2316 86-87 -0.3529078688202887 2316 88-89 -0.45386484296757956 2316 90-91 -0.24856517935964817 2316 92-93 -0.37020597532131205 2316 94-95 -0.2606067817984865 2316 96-97 -0.33493473585931355 2316 98-99 -0.20611759000804852 2316 100-101 -0.717715290590796 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 5.0 4 10.0 5 37.0 6 69.0 7 144.0 8 386.0 9 785.0 10 1268.0 11 2165.0 12 3444.0 13 4842.0 14 6223.0 15 8760.0 16 12213.0 17 17008.0 18 15148.0 19 9046.0 20 7723.0 21 7707.0 22 8295.0 23 9236.0 24 10834.0 25 13041.0 26 15946.0 27 20092.0 28 25622.0 29 32440.0 30 41351.0 31 52623.0 32 67812.0 33 90806.0 34 125476.0 35 181102.0 36 285270.0 37 460036.0 38 412398.0 39 41645.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.225516529185185 20.37628802516627 15.219915928169591 22.17827951747895 2 15.555614710315513 21.23257983004614 39.949553267159246 23.262252192479096 3 19.20079798655055 25.32713279836224 30.395964663178653 25.07610455190855 4 12.957989684637603 17.468110422292995 37.67287071743953 31.90102917562987 5 13.375748822605965 38.27642072213501 34.48464678178964 13.863183673469386 6 31.554270669524175 37.29808624864454 17.43757317262436 13.710069909206931 7 28.07066359753914 33.326033859180086 21.006865356344086 17.596437186936686 8 26.337423550146134 35.83551271188356 19.75930821075323 18.067755527217077 9 26.31828754336365 16.853246918274184 20.57170954856603 36.25675598979614 10-11 24.477589526125172 27.580510604913783 27.65255942835072 20.289340440610324 12-13 25.500918157416784 24.700468722374914 28.3920843579729 21.406528762235403 14-15 22.43942901370208 26.140764666794908 26.602891696730957 24.81691462277205 16-17 21.583180605230208 29.622136693368343 27.16431501382966 21.63036768757179 18-19 21.56271361634868 27.957731022078736 29.747324349287872 20.732231012284714 20-21 22.3110771361886 27.055626513364317 29.89549028106826 20.737806069378824 22-23 22.007884515801425 27.09525055613009 29.776754390150433 21.120110537918052 24-25 21.863099632849657 27.113349506029603 29.928277607847274 21.095273253273465 26-27 21.794271332326964 27.507117702706672 29.634003043215007 21.064607921751353 28-29 21.586100674277624 27.542567086843917 29.750588351112572 21.12074388776589 30-31 21.811280801562624 27.297362997994483 29.780448224545218 21.11090797589767 32-33 21.58960949989729 27.37054190057212 29.7367518311049 21.30309676842569 34-35 21.96577517653092 27.341963454747365 29.624823770436226 21.067437598285494 36-37 22.02340921270651 27.173807678611517 29.525201015966264 21.27758209271571 38-39 21.81032651251048 27.42430855479955 29.65877637039675 21.106588562293226 40-41 22.03373060219155 27.36785482350424 29.50357883507424 21.09483573922997 42-43 21.821269374892154 27.359704946303754 29.403796726609915 21.41522895219418 44-45 21.910777991683624 27.552760883792203 29.262344607839935 21.274116516684238 46-47 22.00048116258524 27.761624621862964 28.79594839003903 21.441945825512768 48-49 22.14698964495927 27.89268868931412 28.47899886037797 21.48132280534864 50-51 22.157185470095346 28.006751343915226 28.373298791418026 21.4627643945714 52-53 22.38086580134414 28.010794515951947 28.139950005298815 21.4683896774051 54-55 22.16082683621537 27.991532944820495 28.277643870375403 21.56999634858873 56-57 22.20869196604137 28.25067264821741 28.128649213891837 21.411986171849374 58-59 22.317455805116094 27.97834868817902 28.179653452442 21.524542054262884 60-61 22.28500997734317 27.914109967247796 28.423549643874395 21.377330411534643 62-63 22.197089820196876 27.940051561744262 28.556371612211084 21.30648700584778 64-65 22.150605898209502 27.968127750173156 28.369506748079242 21.511759603538103 66-67 22.077653011460008 28.02950360394794 28.217146967043377 21.67569641754867 68-69 22.107436875034843 28.00476742720156 28.27339979538034 21.61439590238326 70-71 22.051962545661475 27.95976516453199 28.284600135308143 21.703672154498392 72-73 22.152904999102212 27.84868967093293 28.139597666005777 21.858807663959077 74-75 22.02976276876421 28.16129594462311 27.932567926545858 21.876373360066818 76-77 22.093700135257915 28.013833173196932 28.102632280785993 21.789834410759156 78-79 22.078707751991324 27.994672053544654 27.98826827174737 21.938351922716652 80-81 21.999099954746608 28.189748826098903 28.00501855589949 21.806132663255 82-83 22.116036344450077 28.09012832963043 28.022549410335635 21.77128591558386 84-85 22.078477177017525 28.05744790333837 27.85252436417826 22.011550555465845 86-87 22.064493867688327 27.900423453210454 27.97109106880876 22.06399161029246 88-89 22.19721538454584 27.744974789190014 27.940553819140124 22.11725600712402 90-91 22.06065477012721 27.69574663133704 28.06921927390087 22.17437932463488 92-93 21.971677477843993 27.54110381994932 28.16086931408667 22.326349388120008 94-95 21.834434867512034 27.686888721358144 28.096178273249116 22.382498137880706 96-97 21.833907497246376 27.658536291361525 28.107855757703 22.399700453689107 98-99 21.635582351565876 27.668275816865766 28.023623192154535 22.672518639413823 100-101 21.63342778011223 27.961529409903125 27.957485637701616 22.44755717228303 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 249.0 1 171.5 2 130.5 3 193.5 4 197.5 5 199.5 6 358.5 7 827.0 8 1413.0 9 1779.5 10 1757.0 11 1740.5 12 1954.0 13 2101.0 14 2225.0 15 2370.0 16 2579.5 17 2766.5 18 2933.0 19 3231.0 20 3547.5 21 4021.5 22 4795.5 23 5726.5 24 6886.0 25 8614.5 26 11179.5 27 14200.0 28 17152.5 29 20748.0 30 25312.5 31 29844.0 32 34254.0 33 38981.5 34 44708.5 35 49302.0 36 53691.5 37 58687.5 38 62369.0 39 64701.0 40 67131.0 41 70266.0 42 72408.5 43 75571.5 44 79628.5 45 81458.5 46 80368.5 47 79912.0 48 81000.5 49 78794.5 50 74494.5 51 69661.5 52 64804.5 53 61720.0 54 58393.5 55 52768.0 56 45957.0 57 39934.0 58 35484.5 59 31368.0 60 25813.0 61 20084.0 62 15331.5 63 11596.5 64 8596.5 65 6184.0 66 4374.5 67 3224.5 68 2256.0 69 1418.5 70 952.5 71 628.5 72 428.5 73 357.0 74 287.5 75 207.5 76 146.0 77 100.5 78 53.5 79 30.0 80 17.0 81 11.5 82 7.5 83 1.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05253612360755415 2 0.0 3 0.0 4 0.0 5 0.019387135480416734 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003365124552300314 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001004514791731437 24-25 5.022573958657185E-5 26-27 0.0012305306198710103 28-29 0.003289785942920456 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.01619780101666942 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.7579008855300146E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.7624156772614516E-4 84-85 0.0012305306198710103 86-87 0.0 88-89 0.0 90-91 0.011853274542430955 92-93 8.036118333851496E-4 94-95 0.0 96-97 0.0 98-99 7.533860937985778E-5 100-101 0.014841706047831981 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1991011.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.698676559826204 #Duplication Level Percentage of deduplicated Percentage of total 1 76.30695649291445 44.028104019494 2 13.566670038589049 15.655578131008685 3 4.404966453519774 7.624822039755664 4 1.9560856712652805 4.514542178785838 5 1.0494527478439177 3.0276017331333525 6 0.6085986086029992 2.1069200563526826 7 0.4055284175727183 1.637891710094249 8 0.28787521590457216 1.3288015177654435 9 0.21190374005591503 1.1003908823373356 >10 1.088876348927271 11.430613897279024 >50 0.07795303370571312 3.054295123471877 >100 0.03417714727320428 3.2599634095271752 >500 5.215002683539696E-4 0.19377879699394154 >1k 2.6075013417698443E-4 0.3455320681387825 >5k 1.738334227846563E-4 0.6911644358619304 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7691 0.38628616316032405 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6091 0.3059249798218091 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4286 0.21526751986804693 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 5.022573958657185E-5 0.0 7 0.0 0.0 0.0 5.022573958657185E-5 0.0 8 0.0 0.0 0.0 5.022573958657185E-5 0.0 9 0.0 0.0 0.0 1.5067721875971555E-4 0.0 10-11 5.022573958657185E-5 0.0 0.0 2.7624156772614516E-4 0.0 12-13 5.022573958657185E-5 0.0 0.0 3.013544375194311E-4 1.2556434896642964E-4 14-15 1.004514791731437E-4 0.0 0.0 3.013544375194311E-4 1.5067721875971555E-4 16-17 1.004514791731437E-4 0.0 0.0 3.013544375194311E-4 1.5067721875971555E-4 18-19 1.004514791731437E-4 0.0 0.0 3.013544375194311E-4 1.5067721875971555E-4 20-21 1.004514791731437E-4 0.0 0.0 4.269187864858607E-4 1.5067721875971555E-4 22-23 1.004514791731437E-4 0.0 0.0 5.022573958657184E-4 1.5067721875971555E-4 24-25 1.5067721875971555E-4 0.0 0.0 5.273702656590044E-4 1.5067721875971555E-4 26-27 1.5067721875971555E-4 0.0 0.0 7.784989635918636E-4 2.009029583462874E-4 28-29 3.0135443751943105E-4 0.0 0.0 0.0011803048802844383 2.009029583462874E-4 30-31 3.515801771060029E-4 0.0 0.0 0.0024359483699487347 2.009029583462874E-4 32-33 3.515801771060029E-4 0.0 0.0 0.0038673819481660323 2.009029583462874E-4 34-35 3.515801771060029E-4 0.0 0.0 0.005851298661835621 2.511286979328592E-4 36-37 4.018059166925748E-4 0.0 0.0 0.007860328245298495 2.511286979328592E-4 38-39 4.018059166925748E-4 0.0 0.0 0.010396728094420372 2.511286979328592E-4 40-41 4.018059166925748E-4 0.0 0.0 0.013761852646720685 2.511286979328592E-4 42-43 4.018059166925748E-4 0.0 0.0 0.018809539475171156 2.511286979328592E-4 44-45 4.520316562791466E-4 0.0 0.0 0.02717212511633537 2.511286979328592E-4 46-47 5.022573958657184E-4 0.0 0.0 0.04615745468005952 2.7624156772614516E-4 48-49 5.524831354522903E-4 0.0 0.0 0.08460525833358028 3.013544375194311E-4 50-51 5.524831354522903E-4 0.0 0.0 0.12099380666405157 3.013544375194311E-4 52-53 5.524831354522903E-4 0.0 0.0 0.15939138457798574 3.013544375194311E-4 54-55 5.524831354522903E-4 0.0 0.0 0.20994359147187033 3.013544375194311E-4 56-57 6.52934614625434E-4 0.0 0.0 0.2483662822555978 3.2646730731271704E-4 58-59 6.52934614625434E-4 0.0 0.0 0.29623141208160075 3.515801771060029E-4 60-61 6.52934614625434E-4 0.0 0.0 0.3709924254562129 3.515801771060029E-4 62-63 6.52934614625434E-4 0.0 0.0 0.4249348697721911 3.515801771060029E-4 64-65 6.52934614625434E-4 0.0 0.0 0.4900776540159748 3.515801771060029E-4 66-67 6.52934614625434E-4 0.0 0.0 0.6153406485448849 3.515801771060029E-4 68-69 6.52934614625434E-4 0.0 0.0 0.7508245810796625 3.515801771060029E-4 70-71 6.52934614625434E-4 0.0 0.0 0.8736767401084173 3.515801771060029E-4 72-73 6.52934614625434E-4 0.0 0.0 1.0202605610918272 4.018059166925748E-4 74-75 6.52934614625434E-4 0.0 0.0 1.1646093366636348 4.7714452607243256E-4 76-77 6.52934614625434E-4 0.0 0.0 1.291630232078075 5.022573958657184E-4 78-79 6.52934614625434E-4 0.0 0.0 1.4556926104376118 5.022573958657184E-4 80-81 6.52934614625434E-4 0.0 0.0 1.680201666389588 5.022573958657184E-4 82-83 6.52934614625434E-4 0.0 0.0 1.8905972895177374 5.022573958657184E-4 84-85 7.282732240052917E-4 0.0 0.0 2.1416255359714236 5.022573958657184E-4 86-87 8.036118333851496E-4 0.0 0.0 2.5784136802860456 5.022573958657184E-4 88-89 8.036118333851496E-4 0.0 0.0 3.143553702114152 5.022573958657184E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4840 0.0 46.351345 1 GTATCAA 8165 0.0 37.779327 1 ATCAACG 10175 0.0 29.974234 3 TCAACGC 10305 0.0 29.6422 4 TATCAAC 10490 0.0 29.255295 2 CAACGCA 10580 0.0 28.871725 5 AACGCAG 10770 0.0 28.356686 6 CGCGTGA 35 0.008393047 27.143496 26-27 GTACATG 8160 0.0 24.638594 1 TATACCG 135 1.3420808E-5 24.632685 5 ACGCAGA 12520 0.0 24.356989 7 CGCAGAG 12610 0.0 23.957138 8 TACATGG 8255 0.0 23.709784 2 ACATGGG 8155 0.0 22.777195 3 GAGTACT 7765 0.0 22.358303 12-13 GCAGAGT 13720 0.0 21.91505 9 GTACTTT 8205 0.0 20.839348 14-15 AGAGTAC 12135 0.0 20.510887 10-11 AGTACTT 8120 0.0 20.415607 12-13 CAGAGTA 13150 0.0 19.090273 10-11 >>END_MODULE