FastQCFastQC Report
Thu 26 May 2016
SRR937348_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937348_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2276259
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74830.328741149403473No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54840.2409216174433577No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37800.16606194637780675No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35690.15679235095830485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48300.046.4347421
GTATCAA83500.039.8914381
ATCAACG103300.031.9615153
TCAACGC103200.031.9032344
TATCAAC105450.031.3999562
CAACGCA106550.030.90295
AACGCAG109400.030.1386176
ACGCAGA125950.025.8765957
CGCAGAG126800.025.6656638
GTACATG98400.025.303721
TACATGG99250.024.5544222
ACATGGG99750.023.859853
GCAGAGT137050.022.9834739
GAGTACT73500.021.81393812-13
ATGGGCG2457.503331E-921.3327125
TGGTATC26300.021.3142032
CATGGGA60700.020.0369874
GTACTTT80100.019.95590814-15
CAGAGTA132950.019.56379110-11
AGAGTAC124600.019.54038210-11
Produced by FastQC (version 0.11.5)