##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937348_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2276259 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.700539349871875 33.0 31.0 34.0 30.0 34.0 2 31.807117731330223 33.0 31.0 34.0 30.0 34.0 3 31.853773230550654 33.0 31.0 34.0 30.0 34.0 4 35.469841525063714 37.0 35.0 37.0 33.0 37.0 5 35.48357809897731 37.0 35.0 37.0 33.0 37.0 6 35.50226841497387 37.0 35.0 37.0 33.0 37.0 7 35.38736145579216 37.0 35.0 37.0 33.0 37.0 8 35.39155737550077 37.0 35.0 37.0 33.0 37.0 9 37.01314042031245 39.0 37.0 39.0 33.0 39.0 10-11 36.90673622817087 39.0 37.0 39.0 33.0 39.0 12-13 36.7587144081583 39.0 37.0 39.0 33.0 39.0 14-15 37.93777597364799 40.0 38.0 41.0 33.0 41.0 16-17 37.87067925925828 40.0 38.0 41.0 33.0 41.0 18-19 37.83133883270753 40.0 38.0 41.0 33.0 41.0 20-21 37.71614719590346 40.0 37.5 41.0 32.0 41.0 22-23 37.567626750734426 40.0 37.0 41.0 32.0 41.0 24-25 37.50978579326869 40.0 37.0 41.0 32.0 41.0 26-27 37.53803213957639 40.0 37.0 41.0 32.0 41.0 28-29 37.456088257092006 40.0 37.0 41.0 32.0 41.0 30-31 37.268267582906866 39.5 37.0 41.0 31.5 41.0 32-33 37.05482482441585 39.0 36.0 41.0 31.0 41.0 34-35 37.10526921584933 39.5 36.5 41.0 31.0 41.0 36-37 36.992666036685634 39.5 36.0 41.0 31.0 41.0 38-39 36.88895859390342 40.0 36.0 41.0 30.5 41.0 40-41 36.68821496147846 39.0 36.0 41.0 30.0 41.0 42-43 36.44725622172169 39.0 36.0 41.0 30.0 41.0 44-45 36.24371523627144 39.0 35.0 41.0 30.0 41.0 46-47 35.94435958298243 39.0 35.0 41.0 28.5 41.0 48-49 35.66115257534402 39.0 35.0 40.0 27.5 41.0 50-51 34.707184243972236 38.0 34.0 39.5 25.5 40.5 52-53 34.84562543190384 38.0 34.5 39.5 26.5 40.5 54-55 35.23925616548908 39.0 35.0 40.0 26.5 41.0 56-57 35.03987046289548 38.5 34.5 40.0 26.0 41.0 58-59 34.781781642598666 38.0 34.0 40.0 26.0 41.0 60-61 34.48865353195748 38.0 34.0 40.0 25.0 41.0 62-63 34.18503408443415 38.0 34.0 40.0 24.0 41.0 64-65 33.7709957434545 37.0 33.0 40.0 23.0 41.0 66-67 33.430589401293965 36.5 33.0 39.5 22.0 41.0 68-69 32.95144906620907 36.0 32.0 39.0 21.5 40.5 70-71 32.480729565484424 35.5 32.0 39.0 19.5 40.0 72-73 32.01734556568475 35.0 31.0 38.0 18.0 40.0 74-75 31.523516436398495 35.0 31.0 37.0 15.5 39.5 76-77 31.027800878546774 35.0 30.5 37.0 11.5 39.0 78-79 30.513587865001302 34.0 30.0 36.0 7.5 39.0 80-81 30.0109985287263 34.0 29.5 36.0 6.5 37.5 82-83 29.515404002795815 34.0 29.0 35.0 2.0 37.0 84-85 28.984776117304754 34.0 29.0 35.0 2.0 36.5 86-87 28.50947871046309 34.0 28.5 35.0 2.0 36.0 88-89 28.040604781793284 33.5 27.0 35.0 2.0 36.0 90-91 27.518342815997652 33.0 26.0 35.0 2.0 35.0 92-93 26.999692258218417 33.0 25.5 35.0 2.0 35.0 94-95 26.441077443296216 32.5 25.0 35.0 2.0 35.0 96-97 25.69963545448914 32.0 23.5 34.0 2.0 35.0 98-99 24.760652895826002 32.0 13.0 34.0 2.0 35.0 100-101 22.202065098918887 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.05766668903698147 1101 2 -0.15808404805200738 1101 3 -0.1422572720911468 1101 4 -0.08521535439259509 1101 5 -0.14536854772075003 1101 6 -0.21467666017397136 1101 7 -0.10972667218259602 1101 8 -0.2165600992560286 1101 9 -0.2163270018592982 1101 10-11 -0.2799342829506202 1101 12-13 -0.2916707652513253 1101 14-15 -0.3105066166372126 1101 16-17 -0.36717466575324664 1101 18-19 -0.13064060199596383 1101 20-21 -0.0894296319870378 1101 22-23 -0.1942425213280714 1101 24-25 -0.16604256589445043 1101 26-27 -0.19085416369738084 1101 28-29 -0.3315253742405986 1101 30-31 -0.2599523510151087 1101 32-33 -0.4510500201483154 1101 34-35 -0.2905660584938019 1101 36-37 -0.21509457450272862 1101 38-39 -0.3477913803813095 1101 40-41 -0.32487359118970716 1101 42-43 -0.18431452310412766 1101 44-45 -0.16664814524406069 1101 46-47 -0.21574481560649872 1101 48-49 -0.23953774255883076 1101 50-51 -0.21986003363039686 1101 52-53 -0.15830596059864632 1101 54-55 -0.19916998839225641 1101 56-57 -0.2932783067512972 1101 58-59 -0.24660750906805617 1101 60-61 -0.20027397448740203 1101 62-63 -0.18078391339557953 1101 64-65 -0.3006092722570841 1101 66-67 -0.2961592379718567 1101 68-69 -0.22273763873825203 1101 70-71 -0.26959844501613617 1101 72-73 -0.19906219758152233 1101 74-75 -0.26743784245384106 1101 76-77 -0.28279424836717837 1101 78-79 -0.311095277866432 1101 80-81 -0.28772177863476855 1101 82-83 -0.19798037548789793 1101 84-85 -0.21595024596742363 1101 86-87 -0.04611914278605411 1101 88-89 -0.20854255791183718 1101 90-91 -0.2313527991876967 1101 92-93 -0.08672130944247414 1101 94-95 -0.08473060921937758 1101 96-97 0.09699539877001584 1101 98-99 0.3438091186976955 1101 100-101 0.5884761642673979 1102 1 -0.0166502647384128 1102 2 -0.07875706460040632 1102 3 -0.05711079488674997 1102 4 -0.06760470053797718 1102 5 0.04731150717957888 1102 6 -0.08692776564139848 1102 7 -0.014505039444507872 1102 8 0.004330243398243283 1102 9 2.6233581259305083E-5 1102 10-11 -0.04517074882332395 1102 12-13 -0.040680165972005966 1102 14-15 0.09579458463986157 1102 16-17 0.10076493206788228 1102 18-19 0.09107043849523677 1102 20-21 -0.00263621884435139 1102 22-23 -0.0222180496107498 1102 24-25 0.1341736521585446 1102 26-27 -0.020107646307600646 1102 28-29 -0.051982837567742024 1102 30-31 0.004085570986831044 1102 32-33 0.12453839286879997 1102 34-35 0.19933256205423078 1102 36-37 0.22886823628763864 1102 38-39 0.30693158423634515 1102 40-41 0.14058655362541828 1102 42-43 0.12470923798194633 1102 44-45 0.15533244757900633 1102 46-47 0.12633068563969374 1102 48-49 -0.01729483668275833 1102 50-51 -0.03909302464925446 1102 52-53 -0.02249487327374311 1102 54-55 0.10700792353378574 1102 56-57 0.12930757842783436 1102 58-59 -0.008362103424666145 1102 60-61 0.05698007840069863 1102 62-63 0.03305588875050347 1102 64-65 0.04858768488195864 1102 66-67 -0.008557093963382556 1102 68-69 0.002424617139233476 1102 70-71 -0.03600649213941409 1102 72-73 0.006792236772255933 1102 74-75 -0.08830964368983629 1102 76-77 0.060480904802663105 1102 78-79 0.07554389431464514 1102 80-81 0.04246209904800935 1102 82-83 1.2249023233934508E-4 1102 84-85 0.10728466272163217 1102 86-87 0.10921583637516363 1102 88-89 0.12482290895516357 1102 90-91 0.09267584471808377 1102 92-93 0.16692787805928333 1102 94-95 0.20861198743607545 1102 96-97 0.21883763272282408 1102 98-99 0.03997990953719821 1102 100-101 0.24008291482105193 1103 1 -0.0741310146169063 1103 2 -0.16605938059023018 1103 3 -0.1464734768675271 1103 4 -0.14937845887143908 1103 5 -0.10987784027282288 1103 6 -0.17192269032782548 1103 7 -0.17964255384520555 1103 8 -0.1727016490998352 1103 9 -0.08395654314356449 1103 10-11 -0.10053247330776571 1103 12-13 0.003161361824901121 1103 14-15 -0.1198417532936844 1103 16-17 -0.15244749002623337 1103 18-19 -0.22357677802499154 1103 20-21 -0.12893537888541573 1103 22-23 -0.1562540570113029 1103 24-25 -0.24080166352742083 1103 26-27 -0.39406953882754436 1103 28-29 -0.4010135729089015 1103 30-31 -0.4228479485992196 1103 32-33 -0.34710743695772805 1103 34-35 -0.21728364295200464 1103 36-37 -0.20588010029928228 1103 38-39 -0.3650783711023493 1103 40-41 -0.2858394346033535 1103 42-43 -0.21907129860949937 1103 44-45 -0.2484125727477462 1103 46-47 -0.1467162659295056 1103 48-49 -0.15217375420180446 1103 50-51 -0.23026074026263643 1103 52-53 -0.2271282009961766 1103 54-55 -0.0031908699042304534 1103 56-57 -0.14602289990499173 1103 58-59 -0.2595915826530515 1103 60-61 -0.19723720477265516 1103 62-63 -0.18679609280786025 1103 64-65 -0.2591936488944171 1103 66-67 -0.0048825013547642016 1103 68-69 -0.03698047449695707 1103 70-71 -0.08192368838025743 1103 72-73 -0.17714179070949143 1103 74-75 -0.07365335500104564 1103 76-77 -0.1499564247298295 1103 78-79 -0.1426995079598825 1103 80-81 -0.06959867500835415 1103 82-83 -0.21850456639912252 1103 84-85 -0.09999708239925553 1103 86-87 0.028628564664082035 1103 88-89 0.16474974136925624 1103 90-91 0.005083146465697297 1103 92-93 -0.006439259121378882 1103 94-95 -0.13023788019770421 1103 96-97 -0.15090770531200448 1103 98-99 0.12036588112272639 1103 100-101 0.4515575320593399 1104 1 -0.03357481104710658 1104 2 -0.05101511543040971 1104 3 -0.03367752872126317 1104 4 -0.08027372091600427 1104 5 -0.03531518240752973 1104 6 0.06931135781695019 1104 7 0.03625194935402476 1104 8 0.05276657442053789 1104 9 0.042453225238681114 1104 10-11 0.07719646517620049 1104 12-13 0.15098167215767688 1104 14-15 0.21623667389292933 1104 16-17 0.017808631622621363 1104 18-19 0.11384867630987117 1104 20-21 0.07324371572927646 1104 22-23 -0.06751687993312316 1104 24-25 0.032486759903612494 1104 26-27 0.09839482642134101 1104 28-29 0.11740606328457659 1104 30-31 0.032591144339527034 1104 32-33 -0.017317672081993862 1104 34-35 0.04468001074901906 1104 36-37 -0.01599920773031016 1104 38-39 0.09161934994462939 1104 40-41 0.2211724925803722 1104 42-43 0.14836240916523735 1104 44-45 0.12064973857612671 1104 46-47 0.0915766277569432 1104 48-49 0.17541848783464076 1104 50-51 0.07820774054127355 1104 52-53 0.12969179414280063 1104 54-55 0.01249600290580588 1104 56-57 0.005084676880407812 1104 58-59 0.07980895529897936 1104 60-61 0.05652380323896722 1104 62-63 0.20100295022499637 1104 64-65 0.21965769361155907 1104 66-67 0.20832530902248436 1104 68-69 0.21748956620341175 1104 70-71 0.09398549359919883 1104 72-73 0.20899644232314074 1104 74-75 0.3042937653189419 1104 76-77 0.2358290096358182 1104 78-79 0.17073631182189786 1104 80-81 0.2725142595808947 1104 82-83 0.28760834070729047 1104 84-85 0.3623532082133565 1104 86-87 0.4560598848672406 1104 88-89 0.27876536315435985 1104 90-91 0.48445420907369297 1104 92-93 0.4754004126255609 1104 94-95 0.5994052809127055 1104 96-97 0.8189544179507315 1104 98-99 0.8677868805385955 1104 100-101 1.2009968637552362 1105 1 0.042683141361699484 1105 2 0.12531222944890175 1105 3 0.04310286343808656 1105 4 0.13244937270806645 1105 5 -0.014711258786142878 1105 6 0.04072850415774809 1105 7 -0.0043375127497640165 1105 8 -0.009153943000136167 1105 9 0.13402074094387473 1105 10-11 0.04339244598965308 1105 12-13 0.08512067636621623 1105 14-15 -0.06415269708175231 1105 16-17 -0.12420467525496548 1105 18-19 0.11354759537164227 1105 20-21 0.10161097743902303 1105 22-23 0.11629452210530644 1105 24-25 0.10738506349535015 1105 26-27 0.14975241564453512 1105 28-29 -0.031086852663079867 1105 30-31 0.066590793257987 1105 32-33 0.14305973423658713 1105 34-35 0.1256179307873495 1105 36-37 0.1234311149415106 1105 38-39 0.1989649390233339 1105 40-41 0.06494855832514901 1105 42-43 0.13368251124504837 1105 44-45 0.14857555010363654 1105 46-47 0.11930059908485902 1105 48-49 0.14123518651479117 1105 50-51 0.08757825894421956 1105 52-53 0.18873711851615838 1105 54-55 0.2701612092676058 1105 56-57 0.19213667560536152 1105 58-59 0.1693785382027002 1105 60-61 0.43508900776297565 1105 62-63 0.5093716187957753 1105 64-65 0.3904546170219163 1105 66-67 0.22501492695831615 1105 68-69 0.07020129086929927 1105 70-71 0.04115665725706208 1105 72-73 0.16385318647911618 1105 74-75 0.22246730479755783 1105 76-77 0.27568069352808067 1105 78-79 0.32244377851509753 1105 80-81 0.36961257698775896 1105 82-83 0.3823542850500914 1105 84-85 0.40600202274281116 1105 86-87 0.6605364279941881 1105 88-89 0.41528487764788125 1105 90-91 0.6938325856194147 1105 92-93 0.7675346651926063 1105 94-95 0.9614323902378494 1105 96-97 0.9154460106885409 1105 98-99 0.8795532452793644 1105 100-101 1.2642298811363055 1106 1 -0.0884745906266815 1106 2 -0.0805053950066359 1106 3 -0.11549371734618319 1106 4 0.022484444157207406 1106 5 -0.004024388897946096 1106 6 -0.09988348561413574 1106 7 -0.3970230877538228 1106 8 -0.28754093696469596 1106 9 -0.37726619883331836 1106 10-11 -0.6741685348349691 1106 12-13 -0.3288730256059722 1106 14-15 -0.4256666424761306 1106 16-17 -0.2747809254228386 1106 18-19 -0.4840565348945063 1106 20-21 -0.41794524745922956 1106 22-23 -0.43510035140963765 1106 24-25 -0.35123269779830224 1106 26-27 -0.5402549273774966 1106 28-29 -0.23481996755431567 1106 30-31 -0.3309137151169992 1106 32-33 -0.26829415465861217 1106 34-35 -0.09335212184321051 1106 36-37 -0.4012044900961129 1106 38-39 -0.2508103270860502 1106 40-41 -0.33218793034728833 1106 42-43 -0.22469144033643573 1106 44-45 -0.08029528821023035 1106 46-47 -0.1236429171068707 1106 48-49 -0.019201225520284027 1106 50-51 -0.16170397568637895 1106 52-53 0.049401037284567906 1106 54-55 -0.2514503936426138 1106 56-57 -0.1343194510073218 1106 58-59 -0.13116914834447613 1106 60-61 -0.26594395966647966 1106 62-63 -0.22243408978579993 1106 64-65 -0.1534292603342564 1106 66-67 -0.12206820954772724 1106 68-69 -0.332100168581384 1106 70-71 -0.08809282442179978 1106 72-73 -0.2209416799533841 1106 74-75 0.00245887268309275 1106 76-77 0.202335128506256 1106 78-79 0.19898011711994812 1106 80-81 -0.24224703832875605 1106 82-83 -0.1328447014926013 1106 84-85 -0.30218868736550775 1106 86-87 -0.13538963131524895 1106 88-89 0.27270376865779156 1106 90-91 0.45164455213950916 1106 92-93 0.5398597572810537 1106 94-95 0.5306548383706549 1106 96-97 0.4180073155682784 1106 98-99 0.4174807892289216 1106 100-101 0.9466408378071307 1107 1 0.06254778875807432 1107 2 0.05905490642701494 1107 3 0.06736788903526048 1107 4 0.08964379840301717 1107 5 0.06583310755926419 1107 6 0.012809736833723662 1107 7 0.13042179331906567 1107 8 0.11841149820883601 1107 9 0.06390474045034011 1107 10-11 0.062066077568502465 1107 12-13 0.1307121495612691 1107 14-15 0.11870013252928402 1107 16-17 0.13326258022258486 1107 18-19 0.09212019745517352 1107 20-21 0.12757665301109 1107 22-23 0.17460039101372615 1107 24-25 0.23206319363152517 1107 26-27 0.20585730368535593 1107 28-29 0.2191003672905083 1107 30-31 0.2286371024021676 1107 32-33 0.2332100182500767 1107 34-35 0.2862437435858638 1107 36-37 0.2762578228328536 1107 38-39 0.3907988500168216 1107 40-41 0.3421959400499688 1107 42-43 0.4365916618965713 1107 44-45 0.4273278833426204 1107 46-47 0.5030036547124368 1107 48-49 0.525240433292133 1107 50-51 0.6231272868190416 1107 52-53 0.48355539804560976 1107 54-55 0.5263001432982222 1107 56-57 0.48016475958809934 1107 58-59 0.4377238173237288 1107 60-61 0.6605454450612811 1107 62-63 0.7600066490841684 1107 64-65 0.7675795387524218 1107 66-67 0.6821540609101504 1107 68-69 0.6132527748719667 1107 70-71 0.7982733578047672 1107 72-73 0.9067095658464552 1107 74-75 0.9762027634979837 1107 76-77 0.8974618134432042 1107 78-79 0.9276317011620812 1107 80-81 0.9674709786605007 1107 82-83 1.1752810327277388 1107 84-85 1.1347326813545635 1107 86-87 1.1662485665112214 1107 88-89 1.2637522472718636 1107 90-91 1.2880596789694287 1107 92-93 1.4247030285504145 1107 94-95 1.5357299772613118 1107 96-97 1.670369780001888 1107 98-99 1.952974346007327 1107 100-101 2.201576022623783 1108 1 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1.166862580208086 2309 98-99 1.3028334300233944 2309 100-101 1.4518803257900146 2310 1 0.19935903683253287 2310 2 0.20376538736089955 2310 3 0.2755290797306458 2310 4 0.1282905921374322 2310 5 0.29568399992894 2310 6 0.25496632377606687 2310 7 0.26705978885651405 2310 8 0.11624064392006517 2310 9 0.15443568800961316 2310 10-11 0.26129826563759195 2310 12-13 0.30163498136532496 2310 14-15 0.24151809803773006 2310 16-17 0.28101797449446764 2310 18-19 0.19447443955436938 2310 20-21 0.22806604811501785 2310 22-23 0.3384834308247804 2310 24-25 0.3629750845378723 2310 26-27 0.4589709224825569 2310 28-29 0.4243699731431505 2310 30-31 0.3828996453985738 2310 32-33 0.42537068745532736 2310 34-35 0.25883432596777567 2310 36-37 0.42178410665039223 2310 38-39 0.17961386113638866 2310 40-41 0.2291395638909961 2310 42-43 0.33177831660015045 2310 44-45 0.39592185454414874 2310 46-47 0.33406326506315764 2310 48-49 0.3182715448999076 2310 50-51 0.4622932750937423 2310 52-53 0.24613791768757665 2310 54-55 0.28914687305913134 2310 56-57 0.460737194633019 2310 58-59 0.4023470569425953 2310 60-61 0.5240332741788336 2310 62-63 0.5589441562443724 2310 64-65 0.5305061812460323 2310 66-67 0.4070331468817372 2310 68-69 0.4307979293558475 2310 70-71 0.6854979756042567 2310 72-73 0.7807109003241237 2310 74-75 0.756669492838757 2310 76-77 0.6721033593582639 2310 78-79 0.6216056747328373 2310 80-81 0.5090563519883133 2310 82-83 0.6436463105804116 2310 84-85 0.9580904584552137 2310 86-87 0.8577823863809897 2310 88-89 0.795399855076635 2310 90-91 1.0772519474883389 2310 92-93 1.137090931451894 2310 94-95 1.1489889506057587 2310 96-97 1.1959461857783857 2310 98-99 1.189947975022104 2310 100-101 1.4329514290363363 2311 1 0.4913615432701661 2311 2 0.5961792764964393 2311 3 0.4112889016565262 2311 4 0.38728662462021646 2311 5 0.5086686384126438 2311 6 0.5316507023329038 2311 7 0.593341159688201 2311 8 0.5050356366953892 2311 9 0.6274607079884618 2311 10-11 0.6913591708611548 2311 12-13 0.5790266919585605 2311 14-15 0.7156833251508488 2311 16-17 0.8902445205346154 2311 18-19 0.8031212962151955 2311 20-21 0.8759816889316099 2311 22-23 0.8276665985421872 2311 24-25 0.8539195191162108 2311 26-27 0.8842810487327526 2311 28-29 0.8913365075592452 2311 30-31 1.038927153652466 2311 32-33 0.804591976434267 2311 34-35 0.9540453551005754 2311 36-37 1.057521982111524 2311 38-39 1.1189038854339017 2311 40-41 0.9352441118039039 2311 42-43 0.8963637352680678 2311 44-45 1.0226311562126895 2311 46-47 0.8894014020656016 2311 48-49 0.714840883620127 2311 50-51 0.8056411754981667 2311 52-53 0.7572004494721085 2311 54-55 0.7353485202043757 2311 56-57 0.7962928387592356 2311 58-59 0.9566799449561003 2311 60-61 0.9826942068480875 2311 62-63 0.9122209400126664 2311 64-65 0.9785422501347298 2311 66-67 0.8818176679140279 2311 68-69 1.015846534468814 2311 70-71 1.1414645382732687 2311 72-73 1.0193131173711265 2311 74-75 0.9778749743088788 2311 76-77 1.0350451643969727 2311 78-79 1.2404034019872192 2311 80-81 1.1649019820687947 2311 82-83 1.0800386447559518 2311 84-85 1.0832372727366604 2311 86-87 1.258969816253206 2311 88-89 1.1634400509097311 2311 90-91 1.2231326212801221 2311 92-93 1.5677650763974498 2311 94-95 1.5355007542721815 2311 96-97 1.4167788296059314 2311 98-99 1.4639167938885969 2311 100-101 1.4832204371973958 2312 1 0.46634332700668324 2312 2 0.540488515785281 2312 3 0.47369417708642914 2312 4 0.4648551177709024 2312 5 0.42286275091628767 2312 6 0.48409532489192486 2312 7 0.40644593963573783 2312 8 0.47869127645056864 2312 9 0.7034771754906473 2312 10-11 0.6915923390336971 2312 12-13 0.7392241562546786 2312 14-15 0.8245109264332768 2312 16-17 0.824693296401712 2312 18-19 0.6199257992755349 2312 20-21 0.7031494242857264 2312 22-23 0.823753745146675 2312 24-25 0.7318159050095403 2312 26-27 0.786625481842627 2312 28-29 0.7991348899550417 2312 30-31 0.7849031810497209 2312 32-33 0.7185419910072852 2312 34-35 0.19464940639756634 2312 36-37 0.21630039948254876 2312 38-39 0.5416632850258551 2312 40-41 0.6604153446806222 2312 42-43 0.4650845892464943 2312 44-45 0.5163283290485978 2312 46-47 0.532521119349191 2312 48-49 0.3595599888372689 2312 50-51 0.4598327377249234 2312 52-53 0.488230032684001 2312 54-55 0.3203474272285689 2312 56-57 0.4228721888029625 2312 58-59 0.6239088869555118 2312 60-61 0.4443817614968779 2312 62-63 0.19080043578649253 2312 64-65 0.5953831857220209 2312 66-67 0.46715175418624 2312 68-69 0.4679322966372794 2312 70-71 0.59927385042716 2312 72-73 0.7757634962694056 2312 74-75 0.6213480871039856 2312 76-77 0.6290975543707411 2312 78-79 0.7559983451524737 2312 80-81 0.7825389683701651 2312 82-83 0.9403253687431281 2312 84-85 0.8231636790322021 2312 86-87 0.8628608101325383 2312 88-89 1.030129791510138 2312 90-91 1.1025027028889802 2312 92-93 1.3177705412764915 2312 94-95 1.3774910632200097 2312 96-97 1.335015130975794 2312 98-99 1.306413514961168 2312 100-101 1.3140350685002247 2313 1 0.609748545067724 2313 2 0.5624053348860762 2313 3 0.6398537996916076 2313 4 0.6709637150559686 2313 5 0.7031457970316239 2313 6 0.7480743371187515 2313 7 0.7394198493305453 2313 8 0.5026298535180516 2313 9 0.5572126140195834 2313 10-11 0.6783811134632742 2313 12-13 0.731435574373478 2313 14-15 0.9700028875384987 2313 16-17 0.8026465071459086 2313 18-19 0.8227297850269508 2313 20-21 0.826390013483767 2313 22-23 0.8628506932548845 2313 24-25 0.8329880432592773 2313 26-27 1.0049440531336629 2313 28-29 1.0186280063505393 2313 30-31 1.0203104208687321 2313 32-33 1.1020839184165396 2313 34-35 1.0914771718736418 2313 36-37 1.0227194810267903 2313 38-39 1.0232652875289787 2313 40-41 1.1343972451331084 2313 42-43 1.0667501327142972 2313 44-45 0.8448278958147455 2313 46-47 0.7363552389217389 2313 48-49 0.7597236549583783 2313 50-51 1.011002469119937 2313 52-53 0.9144286463356863 2313 54-55 1.1379607900859838 2313 56-57 1.177671457119125 2313 58-59 1.1017513823824956 2313 60-61 1.0818155729920207 2313 62-63 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2315 46-47 0.6156458321016274 2315 48-49 0.7630346704993798 2315 50-51 0.8076456010086019 2315 52-53 0.7498409170009666 2315 54-55 0.7086175886279023 2315 56-57 0.92375961479447 2315 58-59 0.9103981134823229 2315 60-61 0.9364787362731661 2315 62-63 0.8470637843495794 2315 64-65 1.263141843701419 2315 66-67 1.2839891923435331 2315 68-69 1.132838079437569 2315 70-71 1.256905849129474 2315 72-73 1.156920056327806 2315 74-75 1.2549986318605342 2315 76-77 1.2918243109740537 2315 78-79 1.1749938573317742 2315 80-81 1.1591590446090478 2315 82-83 1.3638952138322082 2315 84-85 1.1945680154565466 2315 86-87 1.2987863555649675 2315 88-89 1.5927004206160866 2315 90-91 1.8005284673054298 2315 92-93 1.8829635716368713 2315 94-95 2.0241535845101204 2315 96-97 1.8744052199211545 2315 98-99 2.244032131306568 2315 100-101 2.040092815397948 2316 1 0.053630727793716915 2316 2 0.23621985707788085 2316 3 0.32454790882790974 2316 4 0.21861687325679213 2316 5 0.206858615653168 2316 6 0.3773988665447021 2316 7 0.44869232128050385 2316 8 0.18012527898508068 2316 9 0.3788590203515838 2316 10-11 -0.11673654778014964 2316 12-13 0.3002042803125704 2316 14-15 0.40273242980151736 2316 16-17 0.3638282260355368 2316 18-19 0.31963399643420587 2316 20-21 0.20145029802681336 2316 22-23 0.32699025551817584 2316 24-25 0.4205956688993595 2316 26-27 0.44731380305919544 2316 28-29 0.5337026882609166 2316 30-31 0.5367840587926693 2316 32-33 0.4604648883593754 2316 34-35 0.572177252725858 2316 36-37 0.48138910754632036 2316 38-39 0.5813051178811861 2316 40-41 0.630242930887519 2316 42-43 0.620465433640554 2316 44-45 0.5191796596695539 2316 46-47 0.5951103276464025 2316 48-49 0.5445379785658062 2316 50-51 0.4877290893556818 2316 52-53 0.34711918095320016 2316 54-55 0.5820078687679526 2316 56-57 0.5263554810059858 2316 58-59 0.6125635924847614 2316 60-61 0.6760048522725768 2316 62-63 0.6600644256484287 2316 64-65 0.6142469888301392 2316 66-67 0.6498870306485927 2316 68-69 0.8125531196880331 2316 70-71 0.6608892709567371 2316 72-73 0.35227920359949394 2316 74-75 0.38285012138664953 2316 76-77 0.6039825006790203 2316 78-79 0.7840664435984159 2316 80-81 0.8884950812228283 2316 82-83 0.7527194859978472 2316 84-85 0.6481903581491224 2316 86-87 0.8758268609562592 2316 88-89 0.8495605258528336 2316 90-91 1.0362834672456849 2316 92-93 1.0660514056798789 2316 94-95 1.2460997472725701 2316 96-97 1.5089963221983638 2316 98-99 2.089513961836378 2316 100-101 1.268417113454536 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8961.0 3 2072.0 4 1533.0 5 1606.0 6 1935.0 7 2263.0 8 2839.0 9 3546.0 10 4550.0 11 5848.0 12 7847.0 13 9853.0 14 10958.0 15 13649.0 16 16686.0 17 21300.0 18 18559.0 19 13823.0 20 13902.0 21 15487.0 22 16524.0 23 18299.0 24 21307.0 25 24067.0 26 27808.0 27 32650.0 28 39297.0 29 46880.0 30 57708.0 31 71586.0 32 90983.0 33 117757.0 34 157487.0 35 218743.0 36 319628.0 37 445631.0 38 352453.0 39 40230.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.53773323506959 19.628462596709102 14.864447568170474 22.969356600050837 2 14.771635345812353 20.30748661924134 41.0210419420838 23.899836092862508 3 18.7613935588414 24.927873818812692 30.1297214467508 26.18101117559511 4 12.155609200605577 16.553700877275645 38.27589486783619 33.01479505428259 5 13.185442286030332 37.81354226022565 34.863354097439526 14.137661356304488 6 31.415360666576515 36.68364441088931 17.96168400781067 13.939310914723505 7 27.942738261312734 32.92078651858619 21.250132697150523 17.88634252295055 8 26.014131019799457 35.253599921778175 20.235449462797504 18.496819595624864 9 26.011461156526877 16.741770771584576 20.751193723669157 36.49557434821939 10-11 24.195152825551094 27.11968359976401 28.13802143264085 20.547142142044045 12-13 25.21448752363894 24.345922342049377 28.57581429499855 21.863775839313128 14-15 22.233425213026862 25.696066847431414 26.809192490673677 25.26131544886805 16-17 21.49620288234298 29.178552732508628 27.33629603136622 21.988948353782174 18-19 21.414693966171207 27.7028784181725 29.77247158785003 21.109956027806263 20-21 22.224027736217927 26.79541302563852 29.799524092932888 21.181035145210664 22-23 21.853464732638464 26.894531052179392 29.629951749111232 21.622052466070908 24-25 21.57345223189094 26.901033507701932 29.846159468998838 21.679354791408286 26-27 21.537319269468167 27.256753614610634 29.562499119246922 21.643427996674276 28-29 21.412126708471764 27.301016527211118 29.624749546000512 21.662107218316606 30-31 21.596876078439838 27.037575459130448 29.702272974030674 21.66327548839904 32-33 21.34425765940522 27.122165520145387 29.64211181907956 21.891465001369838 34-35 21.713715090301655 27.10595756799026 29.508568971892963 21.671758369815127 36-37 21.717267130197826 26.93227891664234 29.453362626600242 21.897091326559597 38-39 21.559455367053005 27.16471207593807 29.577838787714118 21.697993769294808 40-41 21.774815534369623 27.068763907991872 29.445102636493996 21.711317921144506 42-43 21.514536795627457 27.07392725888357 29.381367368818083 22.03016857667089 44-45 21.57080169798543 27.272442973786937 29.257720493098493 21.89903483512914 46-47 21.723092916105045 27.391310093294923 28.863370069861073 22.02222692073896 48-49 21.796396344408635 27.51126982088765 28.604784390546456 22.087549444157258 50-51 21.86364573458463 27.550866228031534 28.588961054979755 21.99652698240408 52-53 21.945536127164765 27.615336808750772 28.415954726016217 22.023172338068246 54-55 21.771670409130806 27.514258623176996 28.532003299388688 22.182067668303514 56-57 21.810763377048463 27.82496097872341 28.353523408252272 22.010752235975843 58-59 21.947612472834987 27.594456752596745 28.436528091351924 22.021402683216337 60-61 21.841852810795796 27.514778311026266 28.64934139824593 21.994027479932008 62-63 21.80578597544223 27.492145176856802 28.804085564737125 21.89798328296384 64-65 21.76945951782357 27.519730772608913 28.655797984360664 22.05501172520685 66-67 21.699571688421983 27.68946188859056 28.399829089217675 22.21113733376978 68-69 21.68068470024112 27.60836798125127 28.556987505616277 22.153959812891337 70-71 21.664621213205226 27.481142386675455 28.616616713574512 22.237619686544804 72-73 21.70196803097177 27.404870357663107 28.487015512963353 22.40614609840177 74-75 21.596914830240372 27.77359482511515 28.280986983091626 22.34850336155285 76-77 21.71686445221715 27.562685303537236 28.3158887909044 22.404561453341216 78-79 21.654676338192214 27.579744399590034 28.322788816463756 22.442790445753992 80-81 21.55157533951017 27.73479502577204 28.30483570270525 22.408793932012543 82-83 21.66630819630556 27.62364530674973 28.372184255673538 22.33786224127117 84-85 21.679944452565415 27.524489127391018 28.233978157917445 22.561588262126126 86-87 21.66413035245961 27.463923572398564 28.376666437693853 22.49527963744797 88-89 21.82108197125546 27.279132027617305 28.324820346625334 22.574965654501902 90-91 21.696688018168043 27.20137678320927 28.452873777330943 22.649061421291744 92-93 21.623340640382864 27.129188151351695 28.53960942620397 22.70786178206147 94-95 21.494730631465625 27.155861911062484 28.47570278427842 22.873704673193476 96-97 21.473478124742666 27.09684578662459 28.51758268421449 22.91209340441825 98-99 21.402422107233292 27.103798304524933 28.459649895409004 23.034129692832764 100-101 21.516193879240205 26.769122531426216 28.812024732406073 22.902658856927506 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3270.0 1 1900.5 2 499.5 3 429.5 4 338.5 5 314.5 6 461.5 7 911.0 8 1471.0 9 1841.5 10 1873.5 11 1825.0 12 1910.5 13 2037.0 14 2201.5 15 2386.5 16 2598.0 17 2766.0 18 2935.0 19 3200.0 20 3573.0 21 4145.0 22 5016.5 23 6118.5 24 7545.0 25 9606.5 26 12390.0 27 15952.0 28 19512.0 29 23447.5 30 28510.0 31 33734.0 32 39034.0 33 44270.0 34 50445.5 35 56145.5 36 61262.0 37 66901.5 38 70798.0 39 73595.5 40 76350.0 41 79588.5 42 82340.5 43 85798.5 44 90143.5 45 92039.5 46 91246.5 47 90942.5 48 92013.5 49 90088.0 50 85465.0 51 80005.5 52 74796.5 53 71129.0 54 67057.0 55 60562.0 56 53215.5 57 46706.5 58 41600.5 59 36732.5 60 30607.5 61 24219.0 62 18493.5 63 13759.0 64 10078.5 65 7520.5 66 5396.5 67 3834.0 68 2738.5 69 1844.0 70 1276.0 71 882.0 72 611.5 73 453.5 74 343.0 75 255.0 76 177.0 77 122.0 78 81.5 79 55.5 80 41.0 81 26.5 82 16.0 83 11.5 84 7.0 85 10.5 86 9.5 87 4.5 88 6.0 89 6.0 90 3.5 91 3.0 92 2.5 93 2.0 94 1.0 95 0.0 96 0.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2714102393444683 2 0.23991118761090016 3 0.23015834314109246 4 0.235693741353686 5 0.23516656057153426 6 0.2649962064949551 7 0.2658748411318747 8 0.25449652258376576 9 0.24746744548840882 10-11 0.25671507504198776 12-13 0.25943884241643855 14-15 0.2432060674993487 16-17 0.2600099549304363 18-19 0.2468743671084881 20-21 0.23887879191251965 22-23 0.23810998660521496 24-25 0.23299193984515823 26-27 0.2405262318567439 28-29 0.258406446718058 30-31 0.2595267058801305 32-33 0.2597683304052834 34-35 0.2663361243162575 36-37 0.23854930392367477 38-39 0.23760477168898617 40-41 0.23316766677254214 42-43 0.2395377678902093 44-45 0.26014175012597424 46-47 0.26530372861787693 48-49 0.27007032152316585 50-51 0.2718056249310821 52-53 0.29432063750214715 54-55 0.26980673113208997 56-57 0.2364625466609907 58-59 0.217681731296834 60-61 0.2133324898440819 62-63 0.22154772369928025 64-65 0.2139694999558486 66-67 0.2151995884475361 68-69 0.2178354923582949 70-71 0.21796728755383285 72-73 0.22095464531935952 74-75 0.22563337476095646 76-77 0.24125110543220257 78-79 0.23422202833684566 80-81 0.22813748347617738 82-83 0.22117430397858942 84-85 0.22172345062666415 86-87 0.23050979699586033 88-89 0.22927970850417284 90-91 0.23980135828128524 92-93 0.2268854291185669 94-95 0.2246668766603449 96-97 0.2354740826944561 98-99 0.24201991073950724 100-101 0.2155730081682269 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2276259.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.639030007634204 #Duplication Level Percentage of deduplicated Percentage of total 1 76.43816784353331 44.058218500619965 2 13.302260690418077 15.33458806208761 3 4.507885273238687 7.79490403605531 4 1.9704466372322451 4.542985314074851 5 1.0479156403640923 3.0200420520207563 6 0.6268645363662996 2.1679118293403308 7 0.4056012384660429 1.6364923368554491 8 0.27859249758032667 1.2846241062347366 9 0.20184938872688316 1.0470962676466293 >10 1.1081336102916868 11.597751465546311 >50 0.07697772838668795 3.054939544393252 >100 0.03431492325658222 3.2863700486076786 >500 6.092259317341568E-4 0.22232305783463344 >1k 2.2845972440025655E-4 0.38258001423223753 >5k 1.5230648293350439E-4 0.5691733644503699 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7483 0.328741149403473 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5484 0.2409216174433577 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3780 0.16606194637780675 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3569 0.15679235095830485 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.393173184598062E-5 0.0 5 0.0 0.0 0.0 4.393173184598062E-5 0.0 6 0.0 0.0 0.0 4.393173184598062E-5 4.393173184598062E-5 7 0.0 0.0 0.0 4.393173184598062E-5 4.393173184598062E-5 8 0.0 0.0 0.0 4.393173184598062E-5 4.393173184598062E-5 9 0.0 0.0 0.0 1.3179519553794186E-4 4.393173184598062E-5 10-11 0.0 0.0 0.0 1.5376106146093216E-4 4.393173184598062E-5 12-13 0.0 0.0 0.0 1.7572692738392247E-4 1.3179519553794186E-4 14-15 0.0 0.0 0.0 1.7572692738392247E-4 1.5376106146093216E-4 16-17 0.0 0.0 0.0 2.196586592299031E-4 1.7572692738392247E-4 18-19 0.0 0.0 0.0 2.196586592299031E-4 1.7572692738392247E-4 20-21 0.0 0.0 0.0 2.416245251528934E-4 2.635903910758837E-4 22-23 0.0 0.0 0.0 3.51453854767845E-4 2.635903910758837E-4 24-25 0.0 0.0 0.0 4.393173184598062E-4 2.635903910758837E-4 26-27 0.0 0.0 0.0 5.271807821517674E-4 2.85556256998874E-4 28-29 6.589759776897093E-5 0.0 0.0 0.0010323956983805446 3.075221229218643E-4 30-31 8.786346369196123E-5 0.0 0.0 0.0022624841900680016 3.075221229218643E-4 32-33 1.0982932961495155E-4 0.0 0.0 0.0037781289387543333 3.075221229218643E-4 34-35 2.416245251528934E-4 0.0 0.0 0.005425568882978607 3.5145385476784494E-4 36-37 3.075221229218643E-4 0.0 0.0 0.007951643464122492 3.5145385476784494E-4 38-39 3.075221229218643E-4 0.0 0.0 0.01058754737488133 3.5145385476784494E-4 40-41 3.075221229218643E-4 0.0 0.0 0.014453539777327624 3.5145385476784494E-4 42-43 4.173514525368159E-4 0.0 0.0 0.0210872312860707 3.5145385476784494E-4 44-45 4.832490503057868E-4 0.0 0.0 0.029126738213885152 3.5145385476784494E-4 46-47 5.930783799207383E-4 0.0 0.0 0.04867635888534652 3.5145385476784494E-4 48-49 6.150442458437287E-4 0.0 0.0 0.088412610340036 3.5145385476784494E-4 50-51 6.150442458437287E-4 0.0 0.0 0.12140534095636744 3.5145385476784494E-4 52-53 6.150442458437287E-4 0.0 0.0 0.15666055576276688 3.5145385476784494E-4 54-55 6.150442458437287E-4 0.0 0.0 0.20518315358665248 3.5145385476784494E-4 56-57 6.150442458437287E-4 0.0 0.0 0.24599573247156847 3.5145385476784494E-4 58-59 6.150442458437287E-4 0.0 0.0 0.2960339750441404 3.5145385476784494E-4 60-61 6.150442458437287E-4 0.0 0.0 0.3681259470033946 3.5145385476784494E-4 62-63 6.150442458437287E-4 0.0 0.0 0.4251932666713234 3.5145385476784494E-4 64-65 6.150442458437287E-4 0.0 0.0 0.49214522600459787 3.5145385476784494E-4 66-67 6.150442458437287E-4 0.0 0.0 0.6083226908712936 3.5145385476784494E-4 68-69 6.150442458437287E-4 0.0 0.0 0.7396566032248527 3.5145385476784494E-4 70-71 6.150442458437287E-4 0.0 0.0 0.8635660528964411 3.5145385476784494E-4 72-73 6.589759776897093E-4 0.0 0.0 1.006497942457339 3.5145385476784494E-4 74-75 7.029077095356899E-4 0.0 0.0 1.1504622277166177 3.5145385476784494E-4 76-77 7.029077095356899E-4 0.0 0.0 1.2834875117462468 3.5145385476784494E-4 78-79 7.029077095356899E-4 0.0 0.0 1.4444753430958428 3.5145385476784494E-4 80-81 7.029077095356899E-4 0.0 0.0 1.6652981932196644 3.5145385476784494E-4 82-83 7.029077095356899E-4 0.0 0.0 1.8729634896556147 3.953855866138256E-4 84-85 7.029077095356899E-4 0.0 0.0 2.1120839060932872 3.953855866138256E-4 86-87 7.029077095356899E-4 0.0 0.0 2.5076452196344965 4.173514525368159E-4 88-89 7.029077095356899E-4 0.0 0.0 2.9933983786555043 4.393173184598062E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4830 0.0 46.434742 1 GTATCAA 8350 0.0 39.891438 1 ATCAACG 10330 0.0 31.961515 3 TCAACGC 10320 0.0 31.903234 4 TATCAAC 10545 0.0 31.399956 2 CAACGCA 10655 0.0 30.9029 5 AACGCAG 10940 0.0 30.138617 6 ACGCAGA 12595 0.0 25.876595 7 CGCAGAG 12680 0.0 25.665663 8 GTACATG 9840 0.0 25.30372 1 TACATGG 9925 0.0 24.554422 2 ACATGGG 9975 0.0 23.85985 3 GCAGAGT 13705 0.0 22.983473 9 GAGTACT 7350 0.0 21.813938 12-13 ATGGGCG 245 7.503331E-9 21.332712 5 TGGTATC 2630 0.0 21.314203 2 CATGGGA 6070 0.0 20.036987 4 GTACTTT 8010 0.0 19.955908 14-15 CAGAGTA 13295 0.0 19.563791 10-11 AGAGTAC 12460 0.0 19.540382 10-11 >>END_MODULE