FastQCFastQC Report
Thu 26 May 2016
SRR937348_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937348_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2276259
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88190.3874339431497031No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69160.30383185744680197No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48220.21183881096131854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50550.046.740931
GTATCAA86800.038.3389661
ATCAACG111700.029.6394063
TCAACGC112700.029.2921184
TATCAAC116050.028.6921272
CAACGCA116700.028.3288065
AACGCAG118600.027.9538466
GTACATG94350.025.0928231
TACATGG92850.024.5043682
ACGCAGA138000.023.5802867
CGCAGAG139400.023.2753128
ACATGGG94750.022.4087773
CTAGACG1553.9088445E-521.451433
GCAGAGT148700.021.404329
GAGTACT83800.021.08805512-13
GTACTTT87350.020.22790114-15
AGAGTAC135250.019.96782710-11
CATGGGG41650.019.6156774
AGTACTT89400.019.6076912-13
TGTACCG2251.6692375E-618.9998385
Produced by FastQC (version 0.11.5)