##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937348_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2276259 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.22216101067585 33.0 31.0 34.0 31.0 34.0 2 32.433612343762285 34.0 31.0 34.0 31.0 34.0 3 32.494422207666176 34.0 31.0 34.0 31.0 34.0 4 36.12973655458364 37.0 35.0 37.0 35.0 37.0 5 36.04981638732675 37.0 35.0 37.0 35.0 37.0 6 36.08678493967514 37.0 35.0 37.0 35.0 37.0 7 36.02678078373331 37.0 35.0 37.0 35.0 37.0 8 35.99944602086142 37.0 35.0 37.0 35.0 37.0 9 37.71578849331293 39.0 37.0 39.0 35.0 39.0 10-11 37.6375678251025 39.0 37.0 39.0 35.0 39.0 12-13 37.542712406628596 39.0 37.0 39.0 35.0 39.0 14-15 38.760210722944976 40.0 38.0 41.0 35.0 41.0 16-17 38.728440392767254 40.0 38.0 41.0 35.0 41.0 18-19 38.71307878409267 40.0 38.0 41.0 34.5 41.0 20-21 38.61451552745096 40.0 38.0 41.0 34.0 41.0 22-23 38.46792763916584 40.0 38.0 41.0 34.0 41.0 24-25 38.42046335676213 40.0 38.0 41.0 34.0 41.0 26-27 38.384121929885836 40.0 38.0 41.0 34.0 41.0 28-29 38.26256216889203 40.0 38.0 41.0 34.0 41.0 30-31 38.36189840435557 40.0 38.0 41.0 34.0 41.0 32-33 38.327639561227436 40.0 38.0 41.0 34.0 41.0 34-35 38.22459680554805 40.0 38.0 41.0 34.0 41.0 36-37 38.076155657155006 40.0 38.0 41.0 33.5 41.0 38-39 37.97020747638999 40.0 37.5 41.0 33.0 41.0 40-41 37.80832585395599 40.0 37.0 41.0 33.0 41.0 42-43 37.619344942732795 40.0 37.0 41.0 33.0 41.0 44-45 37.36917986925038 40.0 37.0 41.0 32.5 41.0 46-47 37.065043125584566 40.0 37.0 41.0 31.5 41.0 48-49 36.781748474141125 40.0 36.0 41.0 31.0 41.0 50-51 36.56781543752271 39.0 36.0 41.0 30.5 41.0 52-53 36.341650708465075 39.0 36.0 41.0 30.0 41.0 54-55 36.132141597243546 39.0 35.0 40.0 30.0 41.0 56-57 35.882551590131 39.0 35.0 40.0 29.0 41.0 58-59 35.62606188487338 38.5 35.0 40.0 28.5 41.0 60-61 35.32346450909145 38.0 34.5 40.0 28.0 41.0 62-63 34.97231378327334 38.0 34.0 40.0 27.5 41.0 64-65 34.58850069346239 37.0 34.0 40.0 26.5 41.0 66-67 34.1869141428985 37.0 33.5 39.0 26.0 41.0 68-69 33.75503468629888 36.0 33.0 39.0 26.0 40.0 70-71 33.35263473971986 36.0 33.0 39.0 25.5 40.0 72-73 32.885988149854654 35.0 32.0 38.0 24.5 40.0 74-75 32.407001795489876 35.0 32.0 37.0 23.5 39.0 76-77 31.11603007390635 34.0 30.5 36.0 22.5 38.5 78-79 31.729489921841058 34.5 31.0 36.0 23.0 39.0 80-81 31.534369111775067 35.0 31.0 36.0 22.5 37.5 82-83 31.188030008887388 34.0 31.0 35.5 21.5 37.0 84-85 30.82066627743152 34.0 31.0 35.0 20.0 37.0 86-87 30.413190019237703 34.0 31.0 35.0 18.5 36.0 88-89 29.963722933110862 34.0 30.0 35.0 16.5 36.0 90-91 29.463753026347177 34.0 29.5 35.0 8.5 35.5 92-93 29.114097956339766 34.0 29.0 35.0 4.5 35.0 94-95 28.69220593965801 34.0 29.0 35.0 2.0 35.0 96-97 28.239206083314773 34.0 29.0 35.0 2.0 35.0 98-99 27.5129499762549 33.0 28.0 35.0 2.0 35.0 100-101 25.041235202145273 31.0 21.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.12319140579441523 1101 2 -0.12658280170402492 1101 3 -0.04916050723854681 1101 4 -0.032459288652532337 1101 5 -0.0612027125175274 1101 6 -0.13364789826184875 1101 7 -0.14910202145345153 1101 8 -0.12520728777135304 1101 9 -0.14186693266708517 1101 10-11 -0.14379713006326256 1101 12-13 -0.1264527711135841 1101 14-15 -0.25792057650775746 1101 16-17 -0.2151066074467991 1101 18-19 -0.1896417622603792 1101 20-21 -0.23047496043105298 1101 22-23 -0.1973467964507023 1101 24-25 -0.24480949852453193 1101 26-27 -0.27289685460699076 1101 28-29 -0.34244773691179375 1101 30-31 -0.3320622909320008 1101 32-33 -0.22015821340129804 1101 34-35 -0.26443049413029485 1101 36-37 -0.2941170402579587 1101 38-39 -0.3329948847424973 1101 40-41 -0.30983742822411386 1101 42-43 -0.26269807564096936 1101 44-45 -0.2718753527678359 1101 46-47 -0.14305198849075396 1101 48-49 -0.27735154427042374 1101 50-51 -0.20088824098336744 1101 52-53 -0.2576878474709119 1101 54-55 -0.32233086600242444 1101 56-57 -0.1506219733419485 1101 58-59 -0.21692041501739112 1101 60-61 -0.2291783146327262 1101 62-63 -0.30120354501941193 1101 64-65 -0.4111865257894536 1101 66-67 -0.25828866397416306 1101 68-69 -0.39423356276501664 1101 70-71 -0.31919751438103816 1101 72-73 -0.32776624055863834 1101 74-75 -0.3303381363057838 1101 76-77 -0.2717196918335816 1101 78-79 -0.4171816805355917 1101 80-81 -0.2682083468839558 1101 82-83 -0.36992638805604017 1101 84-85 -0.4179363717550544 1101 86-87 -0.3002710070621326 1101 88-89 -0.32623391011772895 1101 90-91 -0.29112630559566455 1101 92-93 -0.3682361969316794 1101 94-95 -0.29011972528563135 1101 96-97 -0.3214061730210034 1101 98-99 -0.09701332840898047 1101 100-101 0.5760588827879154 1102 1 -0.08169925745716 1102 2 -0.12880112358008944 1102 3 -0.0937581584619096 1102 4 -0.09832638435727148 1102 5 -0.044075908422755106 1102 6 -0.07568860664744648 1102 7 -0.032526732214712695 1102 8 -0.08555155241575108 1102 9 -0.18357182715828912 1102 10-11 -0.14428393463079914 1102 12-13 -0.22954638089871793 1102 14-15 -0.12056781826864693 1102 16-17 -0.18598069865876 1102 18-19 -0.223063961435102 1102 20-21 -0.01351451200943643 1102 22-23 -0.04683775695527004 1102 24-25 -0.09515920001507538 1102 26-27 -0.2225931003760806 1102 28-29 -0.22006292639623126 1102 30-31 -0.13385305213478915 1102 32-33 -0.06387954253288797 1102 34-35 -0.09976423227228537 1102 36-37 -0.13325878681524017 1102 38-39 -0.1648076001664478 1102 40-41 -0.04960345177688197 1102 42-43 -0.0644386051129544 1102 44-45 -0.09520703140885445 1102 46-47 -0.05069134830212363 1102 48-49 -0.03952276651185116 1102 50-51 0.07462947825967348 1102 52-53 0.03279301793316591 1102 54-55 0.026568394261474282 1102 56-57 -0.13706346888831433 1102 58-59 -0.14248807051045986 1102 60-61 -0.044809764423895615 1102 62-63 0.03726930694620734 1102 64-65 -0.07657634618146858 1102 66-67 -0.043234435865961984 1102 68-69 -0.01683111943453497 1102 70-71 0.02852942593036545 1102 72-73 0.044123270317278696 1102 74-75 0.1000388380597883 1102 76-77 0.013091266957193426 1102 78-79 -0.04524895559716313 1102 80-81 -0.15768671990774052 1102 82-83 0.03243309975759345 1102 84-85 -0.12043562197260016 1102 86-87 -0.13659202032478746 1102 88-89 0.025051357358854887 1102 90-91 -0.058554130724235876 1102 92-93 -0.05238978488079127 1102 94-95 0.0677071170739012 1102 96-97 -0.007996369264557046 1102 98-99 0.18723178470844815 1102 100-101 0.709622838861744 1103 1 -0.0431272386482604 1103 2 -0.042794779620301426 1103 3 -0.09618432594798776 1103 4 -0.07558803225657584 1103 5 -0.10220810558539029 1103 6 -0.14879243233355766 1103 7 -0.1403124745549249 1103 8 -0.17176484383493573 1103 9 -0.12078039740638502 1103 10-11 -0.13324412566371535 1103 12-13 -0.14336278352520537 1103 14-15 -0.226796718189874 1103 16-17 -0.2840517515913987 1103 18-19 -0.23773901384600293 1103 20-21 -0.28273293557987955 1103 22-23 -0.15208092386349392 1103 24-25 -0.2176810224060688 1103 26-27 -0.26985108297355254 1103 28-29 -0.34496992755993716 1103 30-31 -0.3902143182743174 1103 32-33 -0.17349483886489026 1103 34-35 -0.1353214856714331 1103 36-37 -0.2040539459935431 1103 38-39 -0.13234854698021792 1103 40-41 -0.14426715851192995 1103 42-43 -0.09901012549910604 1103 44-45 -0.269328360915118 1103 46-47 -0.18313845100125548 1103 48-49 -0.18369245905643083 1103 50-51 -0.1651125952518555 1103 52-53 -0.024279302188325858 1103 54-55 -0.09539543511180426 1103 56-57 -0.13628595853911918 1103 58-59 -0.23889079172381145 1103 60-61 -0.2846564046974791 1103 62-63 -0.22292230891267906 1103 64-65 -0.4516363718750114 1103 66-67 -0.15258795094872113 1103 68-69 -0.2686628594910374 1103 70-71 -0.2020386448456648 1103 72-73 -0.028669041147395546 1103 74-75 -0.008818075989246665 1103 76-77 0.04409002891587832 1103 78-79 -0.11195362335540437 1103 80-81 -0.16132323855596908 1103 82-83 -0.17546657298851542 1103 84-85 -0.20172534914443574 1103 86-87 -0.15049083759091175 1103 88-89 -0.034961766316300213 1103 90-91 -0.09082736840995054 1103 92-93 -0.01240192482682545 1103 94-95 -0.14692344892011278 1103 96-97 -0.022495625638210726 1103 98-99 0.1574889169267415 1103 100-101 0.6915602970077153 1104 1 -0.016425207457935187 1104 2 0.047272125140992216 1104 3 -0.03849465847682865 1104 4 -0.059807239300624815 1104 5 -0.09014853800576361 1104 6 -0.02040512650931703 1104 7 -0.027869999877474072 1104 8 -0.04208682614261505 1104 9 0.014148437378942447 1104 10-11 -0.016341456258089693 1104 12-13 0.050638203648532 1104 14-15 0.010765181075463204 1104 16-17 -0.14248470714613148 1104 18-19 -0.08260311550502308 1104 20-21 -0.21914574547801635 1104 22-23 -0.18287133397246436 1104 24-25 -0.06970060000220712 1104 26-27 -0.13779782417759634 1104 28-29 -0.15295230566220397 1104 30-31 -0.023252051597793866 1104 32-33 -0.07043247772837447 1104 34-35 -0.12466955471371932 1104 36-37 -0.23425531544864242 1104 38-39 -0.22591258156393934 1104 40-41 -0.2732514799207806 1104 42-43 -0.28246647520644075 1104 44-45 -0.22295229827093266 1104 46-47 -0.32705423524183175 1104 48-49 -0.2531476645452386 1104 50-51 -0.26305965177239443 1104 52-53 -0.39272257484960704 1104 54-55 -0.29725068619268313 1104 56-57 -0.25433303050649414 1104 58-59 -0.3457315523555238 1104 60-61 -0.2385002047593403 1104 62-63 -0.11324093929561485 1104 64-65 -0.23628023784661423 1104 66-67 -0.26312638023825485 1104 68-69 -0.17328299452256601 1104 70-71 -0.22993660891945922 1104 72-73 -0.22118368526734145 1104 74-75 -0.12769950974929145 1104 76-77 -0.13181565636317316 1104 78-79 -0.3899043139905416 1104 80-81 -0.345828721075474 1104 82-83 -0.40950846051475764 1104 84-85 -0.39735653485346134 1104 86-87 -0.39969073732762794 1104 88-89 -0.27224067823147635 1104 90-91 -0.38638909860675597 1104 92-93 -0.31261586154587206 1104 94-95 -0.03283948811309756 1104 96-97 0.10341006577540313 1104 98-99 0.2591822891826432 1104 100-101 0.8369086855379955 1105 1 -0.005070191142785063 1105 2 -0.006931569326809495 1105 3 0.03108843536270456 1105 4 0.05750355214743763 1105 5 0.015624091702704845 1105 6 -0.02088029065895114 1105 7 -0.014389862917653318 1105 8 -0.002948458823873068 1105 9 0.04151201146506622 1105 10-11 0.04287015655601323 1105 12-13 0.05678118002150967 1105 14-15 0.06217980520592192 1105 16-17 0.022064359034011716 1105 18-19 0.05525919082186448 1105 20-21 0.005688076982458767 1105 22-23 -0.0212264218981133 1105 24-25 -0.13727441743093038 1105 26-27 0.0040499547246852785 1105 28-29 -0.061905582032458994 1105 30-31 -0.1183065547888944 1105 32-33 0.007537979457104882 1105 34-35 0.0191935843228066 1105 36-37 -0.014822381845519317 1105 38-39 -0.026294652070482982 1105 40-41 -0.04500126651517178 1105 42-43 -0.04558535940086017 1105 44-45 -0.10209095710143146 1105 46-47 -0.17965905114265723 1105 48-49 0.016629664839591385 1105 50-51 -0.01047353064673473 1105 52-53 0.024622437542809905 1105 54-55 0.054492395631491775 1105 56-57 -0.03102495518745485 1105 58-59 -0.03863412849553782 1105 60-61 -0.14111170641873372 1105 62-63 0.08033306444713872 1105 64-65 0.1273056988661878 1105 66-67 0.12872187395492318 1105 68-69 0.23510265777616013 1105 70-71 0.20611205072853522 1105 72-73 0.2572437482695662 1105 74-75 0.2023718916173891 1105 76-77 0.24590695246418193 1105 78-79 0.17216620523624115 1105 80-81 0.14668417990369065 1105 82-83 0.07361338949484164 1105 84-85 0.11326951197473178 1105 86-87 0.12430543246148673 1105 88-89 0.19376822808615657 1105 90-91 0.3743592880975193 1105 92-93 0.3864020607231531 1105 94-95 0.606352111785764 1105 96-97 0.547012745402089 1105 98-99 0.6586718267626281 1105 100-101 1.1277527451306035 1106 1 -0.1610950438313381 1106 2 -0.052806681827391344 1106 3 -0.01866785514836522 1106 4 -0.027322583932992472 1106 5 -0.11669449255730768 1106 6 -0.016987486753720304 1106 7 -0.09424546128708755 1106 8 -0.07224707661188745 1106 9 -0.08219272020728141 1106 10-11 -0.11775541012283952 1106 12-13 -0.20352694627847256 1106 14-15 -0.2385160780914788 1106 16-17 -0.13316285037040387 1106 18-19 -0.10208586907488382 1106 20-21 -0.0949003001963149 1106 22-23 -0.142798470040006 1106 24-25 -0.11893603841473777 1106 26-27 -0.12451065928291172 1106 28-29 -0.17993012870607572 1106 30-31 -0.10230638374125078 1106 32-33 -0.06689253544308116 1106 34-35 -0.026518030681010885 1106 36-37 -0.1707580358588885 1106 38-39 -0.25461833836109093 1106 40-41 -0.06719904169308677 1106 42-43 -0.0033817579124786334 1106 44-45 0.14814333140383695 1106 46-47 -0.05648951152140569 1106 48-49 -0.16194341025634174 1106 50-51 0.055503822912100986 1106 52-53 -0.004435951843149155 1106 54-55 -0.09945222368392592 1106 56-57 -0.11160650382345949 1106 58-59 0.0016344964885064428 1106 60-61 0.1189239463251468 1106 62-63 0.19438315869038547 1106 64-65 0.2359989282524424 1106 66-67 0.180230667875243 1106 68-69 0.30858719901100784 1106 70-71 0.31230958804331266 1106 72-73 0.3395359738199417 1106 74-75 0.4712517099327158 1106 76-77 0.49523736106092997 1106 78-79 0.35827747039416735 1106 80-81 0.2458834631071447 1106 82-83 0.2325270765805456 1106 84-85 0.37385405394454807 1106 86-87 0.35193811298524125 1106 88-89 0.5405607840391156 1106 90-91 0.5420852029518741 1106 92-93 0.5722129234514028 1106 94-95 0.4684297126287049 1106 96-97 0.5671807328995762 1106 98-99 0.6981433429864303 1106 100-101 1.276960346862193 1107 1 -0.03206087534181279 1107 2 -0.06858001545068504 1107 3 0.040461903375636155 1107 4 -0.02354926070452734 1107 5 -0.07997612392189524 1107 6 -0.04775577272971532 1107 7 -0.03659286279162899 1107 8 0.018797955295447366 1107 9 -0.0419029068806438 1107 10-11 -0.00803196320273969 1107 12-13 0.01208097277643816 1107 14-15 0.08381774721821245 1107 16-17 0.024351510565715273 1107 18-19 0.06527458409593123 1107 20-21 0.0596898993666386 1107 22-23 0.05910680652606004 1107 24-25 -0.004510298737116614 1107 26-27 -0.0975157649224272 1107 28-29 -0.06322354452512258 1107 30-31 0.04019428679366399 1107 32-33 0.05593455189687546 1107 34-35 -0.01244220012385 1107 36-37 -0.03128520144729663 1107 38-39 -0.1078124302133574 1107 40-41 0.0462680484808331 1107 42-43 -0.058100757623478216 1107 44-45 -0.05191323835429529 1107 46-47 -0.09601551001662045 1107 48-49 -0.018392178038169504 1107 50-51 0.012900954787056662 1107 52-53 0.012047854721650708 1107 54-55 0.023431830058989078 1107 56-57 0.04113321086855848 1107 58-59 0.09955379349162996 1107 60-61 0.23915369394824637 1107 62-63 0.20701205628996178 1107 64-65 0.2237354225988213 1107 66-67 0.33476431135746765 1107 68-69 0.3129326482023842 1107 70-71 0.4157535319402328 1107 72-73 0.4267328670788615 1107 74-75 0.3475230762660857 1107 76-77 0.4174557216950525 1107 78-79 0.43308536778407003 1107 80-81 0.36923179645347304 1107 82-83 0.3337183514339337 1107 84-85 0.41553759511292654 1107 86-87 0.5381146442770302 1107 88-89 0.5256698483717628 1107 90-91 0.6396784989431481 1107 92-93 0.8130553861124206 1107 94-95 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90-91 0.594047945111523 2315 92-93 0.5155028662273189 2315 94-95 0.38737565641470795 2315 96-97 0.7895299516189134 2315 98-99 0.8577721075826865 2315 100-101 0.8988910130799326 2316 1 -0.3595526120834478 2316 2 -0.25880316086905 2316 3 -0.1005808858441668 2316 4 0.043140332255831026 2316 5 0.13229197776946222 2316 6 0.04092866123853156 2316 7 0.15205712413231964 2316 8 0.15272034460696204 2316 9 0.2864701672818839 2316 10-11 0.34608768440054405 2316 12-13 0.19634011362798276 2316 14-15 0.2791805874979758 2316 16-17 0.3595817354853281 2316 18-19 0.36335816483738625 2316 20-21 0.3182601857379339 2316 22-23 0.09568374958706016 2316 24-25 0.4891155579747064 2316 26-27 0.37152855425305376 2316 28-29 0.3979048669131444 2316 30-31 0.4667457574750813 2316 32-33 0.22909575369549628 2316 34-35 0.24231903808034616 2316 36-37 0.2886999022221701 2316 38-39 0.19479909353263736 2316 40-41 0.08532915338994229 2316 42-43 0.0872934190979251 2316 44-45 0.06913701631189895 2316 46-47 -0.007441675254135305 2316 48-49 -0.5914265363726798 2316 50-51 -0.46014682522189787 2316 52-53 -0.22161946517157816 2316 54-55 0.05062483373756521 2316 56-57 -0.18495892748644138 2316 58-59 -0.1623105634949482 2316 60-61 -0.24786558833202577 2316 62-63 -0.2461137537628204 2316 64-65 -0.17578519896872535 2316 66-67 -0.15611010209231324 2316 68-69 -0.11868132831141764 2316 70-71 -0.1397243545550353 2316 72-73 -0.12668971750937175 2316 74-75 0.18862859597290083 2316 76-77 0.01539362254516874 2316 78-79 -0.1698729881587333 2316 80-81 0.11604112457481719 2316 82-83 0.1593134846080524 2316 84-85 0.011810171463825014 2316 86-87 -0.24569760665874085 2316 88-89 -0.24607182414168705 2316 90-91 -0.2754158732853931 2316 92-93 -0.0016837437713697057 2316 94-95 0.219223911564022 2316 96-97 0.5734809500136713 2316 98-99 0.5283177692557182 2316 100-101 0.293921146159402 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 17.0 5 45.0 6 91.0 7 198.0 8 481.0 9 865.0 10 1551.0 11 2393.0 12 3906.0 13 5445.0 14 7144.0 15 9933.0 16 13589.0 17 19179.0 18 17584.0 19 10650.0 20 9290.0 21 9627.0 22 10269.0 23 11528.0 24 13163.0 25 15907.0 26 19931.0 27 24679.0 28 30526.0 29 39466.0 30 49215.0 31 62358.0 32 80833.0 33 107373.0 34 146998.0 35 211240.0 36 329379.0 37 521543.0 38 447511.0 39 42342.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.16429540799527 20.30526612419994 15.204220742252398 22.3262177255524 2 15.517873844760196 21.187088112556612 39.92089652363813 23.374141519045065 3 19.092774591995024 25.220592208531627 30.417979676302213 25.268653523171132 4 12.88227745612428 17.40667472374629 37.63908237155789 32.07196544857154 5 13.291954499684563 38.28101523413543 34.54580520902344 13.881225057156566 6 31.598029925417098 37.2640811085206 17.407685153578743 13.730203812483552 7 28.065259709022566 33.308995153890656 20.989922500031852 17.635822637054922 8 26.35372336803501 35.78138515871876 19.745907649349217 18.11898382389702 9 26.25751287529231 16.814738568853542 20.544235080454378 36.38351347539977 10-11 24.460072456748573 27.531038379056472 27.65035719863486 20.35853196556009 12-13 25.478615916863806 24.656493362280248 28.373322062693955 21.49156865816199 14-15 22.396616553740152 26.07427362176273 26.5794885379915 24.94962128650562 16-17 21.598860235148987 29.555797472958922 27.099091096399842 21.746251195492253 18-19 21.58882183442218 27.963865271922046 29.668306638216478 20.7790062554393 20-21 22.269609916973423 27.036576241983006 29.87436842644005 20.819445414603525 22-23 22.015604681404422 27.045720134959407 29.685116683653746 21.25355849998243 24-25 21.815523528588685 27.140793276987523 29.863702950675485 21.179980243748304 26-27 21.81502471169687 27.44116419549698 29.612498627127952 21.1313124656782 28-29 21.567526971050974 27.547496573770957 29.66353211649025 21.22144433868782 30-31 21.75611965549501 27.237895285296904 29.761539970429453 21.244445088778637 32-33 21.60562572185327 27.298145773393976 29.718103256263895 21.378125248488857 34-35 21.946360234050694 27.302890400433345 29.565813907819805 21.18493545769616 36-37 21.970044263426143 27.17149655893652 29.494233629440593 21.364225548196742 38-39 21.78432783971332 27.439712702468732 29.576849431728945 21.199110026089002 40-41 22.03432608129792 27.3082680672112 29.441155053854846 21.216250797636032 42-43 21.82827955512838 27.32456405327475 29.324816696416313 21.522339695180555 44-45 21.895784546154857 27.489093381652456 29.204674079864933 21.41044799232775 46-47 22.02706282545176 27.66616189106776 28.801028353979053 21.50574692950143 48-49 22.144140890821298 27.829609899400726 28.48236953703423 21.543879672743742 50-51 22.190422970321038 27.9228330343779 28.32614829858992 21.56059569671114 52-53 22.378758304744757 27.931641346613016 28.15894412718412 21.530656221458102 54-55 22.15721058104548 27.88722636571673 28.238328766629806 21.717234286607983 56-57 22.209247717417043 28.146928798524247 28.070773141369237 21.573050342689474 58-59 22.317912856138076 27.892102787951632 28.186093937464936 21.603890418445353 60-61 22.236990606077782 27.856100733703855 28.439338405691096 21.467570254527274 62-63 22.151301763112194 27.872157781693556 28.53256154066826 21.44397891452598 64-65 22.122799788073234 27.867381465545645 28.387950750749695 21.621867995631426 66-67 22.07341080254927 27.987698236448487 28.16696166824601 21.77192929275623 68-69 22.11754022718856 27.903459140633817 28.276505441603966 21.702495190573657 70-71 22.06482214897338 27.838791631356536 28.260777881603104 21.83560833806698 72-73 22.11453090355711 27.796199817331857 28.119471466120505 21.969797812990524 74-75 22.037167123776335 28.12215130176311 27.92727013929434 21.913411435166207 76-77 22.080989026292702 27.939768717004526 28.08320582148165 21.896036435221124 78-79 22.011445094780516 27.93392579666901 28.006588881142257 22.048040227408215 80-81 21.976489918210042 28.11327365203629 27.969594828353117 21.940641601400547 82-83 22.093406383402154 28.024528972470915 27.995797468376676 21.88626717575026 84-85 22.060415741806295 27.96219826121491 27.887997093458104 22.08938890352069 86-87 22.049424076961365 27.846347889234046 27.969620328793866 22.134607705010723 88-89 22.172938141046338 27.68463518430899 27.94908224415587 22.1933444304888 90-91 22.039708970765272 27.65994745305484 28.05519186664675 22.24515170953314 92-93 22.007289377112357 27.58461621596449 28.069483685230008 22.338610721693144 94-95 21.874597750080284 27.65959409715678 28.030970992316778 22.434837160446154 96-97 21.851533590861145 27.544668686647693 28.101437490197732 22.502360232293427 98-99 21.713475235880658 27.564132696791265 27.978101769703507 22.744290297624563 100-101 21.67326708964734 27.679399364804024 28.07476568992709 22.572567855621543 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 229.0 1 170.0 2 157.0 3 226.5 4 209.0 5 212.5 6 400.0 7 900.0 8 1570.0 9 1991.0 10 2013.5 11 1955.0 12 2079.5 13 2261.5 14 2464.5 15 2623.5 16 2865.0 17 3104.0 18 3239.5 19 3465.0 20 3823.5 21 4358.0 22 5223.0 23 6434.5 24 7830.5 25 9680.0 26 12400.5 27 16033.5 28 19606.5 29 23490.0 30 28624.5 31 33740.5 32 38965.0 33 44253.0 34 50277.0 35 56045.5 36 61456.0 37 67044.0 38 71104.5 39 73998.5 40 76458.5 41 79752.5 42 82816.0 43 86376.0 44 90818.0 45 92960.0 46 91495.5 47 90860.5 48 92500.5 49 90728.5 50 86261.5 51 80378.0 52 74795.0 53 71006.0 54 67209.0 55 61103.0 56 52828.0 57 45750.0 58 40837.5 59 36007.5 60 29921.5 61 23577.5 62 17938.0 63 13454.0 64 9849.5 65 7171.5 66 5066.5 67 3763.5 68 2618.5 69 1645.0 70 1140.5 71 760.5 72 516.0 73 422.5 74 341.5 75 239.5 76 181.0 77 113.0 78 61.0 79 39.0 80 31.5 81 24.0 82 13.0 83 5.5 84 4.0 85 2.5 86 2.0 87 1.0 88 1.0 89 1.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06976359017141723 2 0.0 3 0.0 4 0.0 5 0.0024162452515289344 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.9769279330691277E-4 12-13 0.006084544860668316 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.347029050736317E-4 24-25 7.688053073046609E-4 26-27 3.9538558661382555E-4 28-29 0.0030093236314496724 30-31 3.294879888448547E-4 32-33 0.0 34-35 0.0 36-37 2.196586592299031E-5 38-39 5.491466480747577E-4 40-41 6.589759776897093E-5 42-43 0.014124051788482769 44-45 1.5376106146093216E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 4.393173184598062E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 6.589759776897093E-5 82-83 5.271807821517674E-4 84-85 6.37010111766719E-4 86-87 0.0 88-89 0.0 90-91 0.008742414637350144 92-93 0.0030752212292186436 94-95 0.0 96-97 0.0 98-99 8.786346369196123E-5 100-101 0.011795670000645797 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2276259.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.925931130482 #Duplication Level Percentage of deduplicated Percentage of total 1 75.19587980413063 42.053995952218116 2 13.906664154424664 15.554862835101929 3 4.691886516048602 7.871943665057138 4 2.148164446014138 4.805523874589468 5 1.1209339086741215 3.1344636289165457 6 0.6634240085051735 2.2261563245981186 7 0.4459082631541252 1.7456484370969312 8 0.29957207107278977 1.340307761234616 9 0.2210921643985645 1.1128306643678991 >10 1.180234431825598 11.963441296593103 >50 0.08653664341574167 3.281274383946617 >100 0.038525768482328984 3.6045214223126574 >500 7.066919121467828E-4 0.23261129977673992 >1k 3.1408529428731167E-4 0.38143510474984427 >5k 1.5704264714365583E-4 0.6909833494403408 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8819 0.3874339431497031 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6916 0.30383185744680197 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4822 0.21183881096131854 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.393173184598062E-5 2 0.0 0.0 0.0 0.0 4.393173184598062E-5 3 0.0 0.0 0.0 4.393173184598062E-5 4.393173184598062E-5 4 0.0 0.0 0.0 4.393173184598062E-5 4.393173184598062E-5 5 0.0 0.0 0.0 4.393173184598062E-5 4.393173184598062E-5 6 0.0 0.0 0.0 4.393173184598062E-5 4.393173184598062E-5 7 0.0 0.0 0.0 4.393173184598062E-5 4.393173184598062E-5 8 0.0 0.0 0.0 4.393173184598062E-5 4.393173184598062E-5 9 0.0 0.0 0.0 8.786346369196123E-5 4.393173184598062E-5 10-11 0.0 0.0 0.0 1.0982932961495155E-4 4.393173184598062E-5 12-13 1.0982932961495155E-4 0.0 0.0 1.3179519553794186E-4 1.0982932961495155E-4 14-15 1.7572692738392247E-4 0.0 0.0 1.3179519553794186E-4 1.3179519553794186E-4 16-17 1.7572692738392247E-4 0.0 0.0 1.3179519553794186E-4 1.3179519553794186E-4 18-19 2.196586592299031E-4 0.0 0.0 1.3179519553794186E-4 1.3179519553794186E-4 20-21 2.635903910758837E-4 0.0 0.0 1.3179519553794186E-4 1.7572692738392247E-4 22-23 2.635903910758837E-4 0.0 0.0 2.196586592299031E-4 1.7572692738392247E-4 24-25 2.635903910758837E-4 0.0 0.0 3.075221229218643E-4 1.7572692738392247E-4 26-27 2.635903910758837E-4 0.0 0.0 3.953855866138256E-4 1.7572692738392247E-4 28-29 3.075221229218643E-4 0.0 0.0 9.006005028426027E-4 2.196586592299031E-4 30-31 3.5145385476784494E-4 0.0 0.0 0.0021526548604530503 2.196586592299031E-4 32-33 3.5145385476784494E-4 0.0 0.0 0.003734197206908353 2.196586592299031E-4 34-35 3.734197206908353E-4 0.0 0.0 0.005359671285209635 2.196586592299031E-4 36-37 3.953855866138256E-4 0.0 0.0 0.007929677598199502 2.196586592299031E-4 38-39 3.953855866138256E-4 0.0 0.0 0.01063147910672731 2.635903910758837E-4 40-41 3.953855866138256E-4 0.0 0.0 0.014365676313635663 2.635903910758837E-4 42-43 5.052149162287771E-4 0.0 0.0 0.0210872312860707 2.635903910758837E-4 44-45 5.71112513997748E-4 0.0 0.0 0.028994943018347208 2.85556256998874E-4 46-47 5.71112513997748E-4 0.0 0.0 0.04858849542165457 3.2948798884485463E-4 48-49 5.71112513997748E-4 0.0 0.0 0.08843457620595899 3.5145385476784494E-4 50-51 5.71112513997748E-4 0.0 0.0 0.12186662414075025 3.5145385476784494E-4 52-53 5.71112513997748E-4 0.0 0.0 0.1570779072153037 3.5145385476784494E-4 54-55 5.71112513997748E-4 0.0 0.0 0.20592999302803416 3.5145385476784494E-4 56-57 6.150442458437287E-4 0.0 0.0 0.24700616230402603 3.734197206908353E-4 58-59 6.150442458437287E-4 0.0 0.0 0.29713226834028994 3.953855866138256E-4 60-61 6.150442458437287E-4 0.0 0.0 0.36966355761800396 3.953855866138256E-4 62-63 6.150442458437287E-4 0.0 0.0 0.4271482287384696 3.953855866138256E-4 64-65 6.150442458437287E-4 0.0 0.0 0.4945175395242808 3.953855866138256E-4 66-67 7.029077095356899E-4 0.0 0.0 0.6114418438323582 3.953855866138256E-4 68-69 7.029077095356899E-4 4.393173184598062E-5 0.0 0.7438740494820668 3.953855866138256E-4 70-71 7.468394413816706E-4 4.393173184598062E-5 0.0 0.8692112804386496 4.173514525368159E-4 72-73 7.468394413816706E-4 4.393173184598062E-5 0.0 1.013592917150465 4.612831843827965E-4 74-75 7.468394413816706E-4 4.393173184598062E-5 0.0 1.1595341303428124 4.832490503057868E-4 76-77 7.468394413816706E-4 4.393173184598062E-5 0.0 1.294294717780358 4.832490503057868E-4 78-79 7.468394413816706E-4 4.393173184598062E-5 0.0 1.4572375111971003 4.832490503057868E-4 80-81 7.468394413816706E-4 4.393173184598062E-5 0.0 1.679685835399223 4.832490503057868E-4 82-83 7.907711732276512E-4 4.393173184598062E-5 0.0 1.8886251520587067 4.832490503057868E-4 84-85 8.127370391506415E-4 4.393173184598062E-5 0.0 2.1292831791109887 4.832490503057868E-4 86-87 8.347029050736318E-4 4.393173184598062E-5 0.0 2.529676983155256 5.052149162287771E-4 88-89 8.347029050736318E-4 4.393173184598062E-5 0.0 3.0236234101655395 5.271807821517674E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5055 0.0 46.74093 1 GTATCAA 8680 0.0 38.338966 1 ATCAACG 11170 0.0 29.639406 3 TCAACGC 11270 0.0 29.292118 4 TATCAAC 11605 0.0 28.692127 2 CAACGCA 11670 0.0 28.328806 5 AACGCAG 11860 0.0 27.953846 6 GTACATG 9435 0.0 25.092823 1 TACATGG 9285 0.0 24.504368 2 ACGCAGA 13800 0.0 23.580286 7 CGCAGAG 13940 0.0 23.275312 8 ACATGGG 9475 0.0 22.408777 3 CTAGACG 155 3.9088445E-5 21.45143 3 GCAGAGT 14870 0.0 21.40432 9 GAGTACT 8380 0.0 21.088055 12-13 GTACTTT 8735 0.0 20.227901 14-15 AGAGTAC 13525 0.0 19.967827 10-11 CATGGGG 4165 0.0 19.615677 4 AGTACTT 8940 0.0 19.60769 12-13 TGTACCG 225 1.6692375E-6 18.999838 5 >>END_MODULE