FastQCFastQC Report
Thu 26 May 2016
SRR937347_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937347_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1277938
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52060.4073750056732017No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35960.2813908029967025No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27220.21299937868660296No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25590.20024445630382695No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22140.17324784144457714No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21350.16706600789709675No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT16910.13232253833910565No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC14910.11667232682649704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG55150.028.2742311
GTATCAA75950.027.7292691
TACATGG55100.027.4241662
ACATGGG54900.026.0516383
GTATAGG3500.024.4493271
GACGCGA1000.001651615323.7552578
ATCAACG89250.023.265593
TATCAAC89900.023.098282
CAACGCA90100.023.0461045
GGTATCA54950.023.0131681
TCAACGC90250.022.956054
AACGCAG92100.022.4878776
CATGGGG26000.021.7486114
GAGTACT42950.021.40042512-13
AGTACTT45400.021.13489312-13
AGAGTAC73950.020.3325210-11
ACTACGT1656.3126485E-520.1559778
CGCAGAG101800.020.0682938
ACGCAGA102750.019.9752247
GTCTAGG4050.019.9552141
Produced by FastQC (version 0.11.5)