##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937347_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1277938 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.775519626147748 33.0 31.0 34.0 30.0 34.0 2 31.901054667753836 33.0 31.0 34.0 30.0 34.0 3 31.940841417971765 33.0 31.0 34.0 30.0 34.0 4 35.559264220955946 37.0 35.0 37.0 33.0 37.0 5 35.56840159694758 37.0 35.0 37.0 33.0 37.0 6 35.60375542475457 37.0 35.0 37.0 33.0 37.0 7 35.54531988249821 37.0 35.0 37.0 33.0 37.0 8 35.53179262217729 37.0 35.0 37.0 33.0 37.0 9 37.15186573996547 39.0 37.0 39.0 34.0 39.0 10-11 37.01868557003548 39.0 37.0 39.0 33.0 39.0 12-13 36.88446857359277 39.0 37.0 39.0 33.0 39.0 14-15 38.004127743286446 40.0 38.0 41.0 33.0 41.0 16-17 37.92425180251311 40.0 38.0 41.0 33.0 41.0 18-19 37.850669203044276 40.0 38.0 41.0 33.0 41.0 20-21 37.72387705819844 40.0 37.5 41.0 32.5 41.0 22-23 37.796062876289774 40.0 38.0 41.0 33.0 41.0 24-25 37.758563795739704 40.0 38.0 41.0 33.0 41.0 26-27 37.673711870215925 40.0 37.0 41.0 32.5 41.0 28-29 37.53905745036144 40.0 37.0 41.0 32.0 41.0 30-31 37.29498027290839 39.5 37.0 41.0 32.0 41.0 32-33 37.05365870644742 39.0 36.0 41.0 31.0 41.0 34-35 37.070542937137795 39.0 36.0 41.0 31.0 41.0 36-37 36.933846164680915 39.0 36.0 41.0 31.0 41.0 38-39 36.79062560155501 39.0 36.0 41.0 30.5 41.0 40-41 36.57267566971167 39.0 35.5 41.0 30.0 41.0 42-43 36.30304482690083 39.0 35.0 40.5 30.0 41.0 44-45 36.05968834168794 39.0 35.0 40.0 29.5 41.0 46-47 35.66549081410835 39.0 35.0 40.0 27.5 41.0 48-49 35.32392220905866 39.0 35.0 40.0 26.5 41.0 50-51 34.36659407576893 37.5 34.0 39.5 25.0 40.5 52-53 34.49687856531381 38.0 34.0 39.5 25.5 40.5 54-55 34.80328857894514 38.0 34.0 40.0 25.5 41.0 56-57 34.524579830946415 38.0 34.0 40.0 24.5 41.0 58-59 34.246031106360405 38.0 34.0 40.0 23.0 41.0 60-61 33.893528872292706 38.0 33.0 40.0 22.5 41.0 62-63 33.51101540137315 37.0 33.0 40.0 22.0 41.0 64-65 33.07679558789237 36.5 32.0 39.5 20.0 41.0 66-67 32.62171873752874 36.0 31.5 39.0 18.5 40.5 68-69 32.10438651953381 35.5 31.0 39.0 15.0 40.0 70-71 31.612204191439645 35.0 31.0 38.0 10.5 40.0 72-73 31.098413225054735 35.0 30.0 37.5 7.5 40.0 74-75 30.51453748147406 34.5 29.5 37.0 4.5 39.0 76-77 29.96130367826921 34.0 29.0 36.5 2.0 39.0 78-79 29.376926345409558 34.0 29.0 36.0 2.0 38.5 80-81 28.740920921046246 34.0 28.0 35.5 2.0 37.0 82-83 28.12997578912279 33.5 26.5 35.0 2.0 37.0 84-85 27.496322591549827 33.0 26.0 35.0 2.0 36.0 86-87 26.86035707522587 32.5 25.0 35.0 2.0 36.0 88-89 26.238962688330734 32.0 24.5 35.0 2.0 35.5 90-91 25.60229682504159 32.0 21.5 34.0 2.0 35.0 92-93 24.847626801926225 31.0 19.0 34.0 2.0 35.0 94-95 23.98298039498004 31.0 9.5 34.0 2.0 35.0 96-97 23.03790089973066 31.0 2.0 34.0 2.0 35.0 98-99 21.769434823911645 30.0 2.0 34.0 2.0 35.0 100-101 18.685259770035792 24.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11638437204049623 1101 2 0.13908785895666043 1101 3 0.03376098243711212 1101 4 -0.08749598129349323 1101 5 -0.06565405026013593 1101 6 -0.06903918493378569 1101 7 0.10365808827891243 1101 8 0.02565413343549494 1101 9 0.042849478711460165 1101 10-11 0.1077331896613245 1101 12-13 0.14694260376176516 1101 14-15 0.16309107051583993 1101 16-17 0.2987942829242698 1101 18-19 0.2568781438725125 1101 20-21 0.32812752497986253 1101 22-23 0.3267585049333661 1101 24-25 0.15663453341765177 1101 26-27 0.20730804633614497 1101 28-29 0.19081003872240387 1101 30-31 0.1923530133821103 1101 32-33 0.04493863719387292 1101 34-35 0.1395960685462967 1101 36-37 0.16237551259209937 1101 38-39 0.13211681371107176 1101 40-41 0.07421154836905686 1101 42-43 0.27312874476173477 1101 44-45 0.2206340871744601 1101 46-47 0.22849833691712007 1101 48-49 0.3404370724767105 1101 50-51 0.1190902357560617 1101 52-53 0.1746548326682955 1101 54-55 0.20224025071030383 1101 56-57 0.216243269732999 1101 58-59 0.1485172240534638 1101 60-61 0.37703796601228134 1101 62-63 0.5010258208392955 1101 64-65 0.3789790958245547 1101 66-67 0.5335045112193768 1101 68-69 0.4630277620578376 1101 70-71 0.44961711921022385 1101 72-73 0.6538663614471361 1101 74-75 0.5826358988796017 1101 76-77 0.6594130412124102 1101 78-79 0.7677068864971908 1101 80-81 0.9541552448684101 1101 82-83 0.7426016982781078 1101 84-85 0.9176014059336204 1101 86-87 1.1404325817755705 1101 88-89 1.1755739293011125 1101 90-91 1.2002052711801845 1101 92-93 1.416212537487553 1101 94-95 1.4193806383268779 1101 96-97 1.4895860556785365 1101 98-99 1.7326816877789675 1101 100-101 1.8693142811154289 1102 1 0.04340238666289764 1102 2 0.10123467542051756 1102 3 -0.06941004755114122 1102 4 0.00525310149262026 1102 5 -0.03640809631735209 1102 6 -0.0660937267417907 1102 7 -0.10112029924582089 1102 8 -0.2214794218964471 1102 9 -0.06690635739686002 1102 10-11 -0.10862036214354731 1102 12-13 -0.01784940797673329 1102 14-15 0.18874009371042888 1102 16-17 0.20477971585037125 1102 18-19 0.1563972621484453 1102 20-21 0.15639822957763272 1102 22-23 0.10672710699912358 1102 24-25 0.07855315006431596 1102 26-27 0.17643352626694053 1102 28-29 0.04542250361008371 1102 30-31 0.21315575851133417 1102 32-33 0.30208956282204014 1102 34-35 0.21493232267130935 1102 36-37 0.3604658173848989 1102 38-39 0.2299888083443733 1102 40-41 0.31295191949632795 1102 42-43 0.4005674732606579 1102 44-45 0.47690024575707923 1102 46-47 0.49160876105433715 1102 48-49 0.49031760126218416 1102 50-51 0.4635320291267533 1102 52-53 0.5330507255787467 1102 54-55 0.7329909771621317 1102 56-57 0.6948947492050479 1102 58-59 0.6331899835791788 1102 60-61 0.4821822017134849 1102 62-63 0.5798669097159674 1102 64-65 0.608333628526708 1102 66-67 0.7437390390343239 1102 68-69 0.8485769896156334 1102 70-71 0.7853242009197743 1102 72-73 0.8945197704398105 1102 74-75 0.9571706066217729 1102 76-77 0.8913923674477822 1102 78-79 0.9301626106840892 1102 80-81 1.1069095375907487 1102 82-83 1.1305330884896527 1102 84-85 1.1547983910864552 1102 86-87 1.2873822909066774 1102 88-89 1.2272741320506206 1102 90-91 1.1550924103604032 1102 92-93 1.2269891522508054 1102 94-95 1.2044722675039061 1102 96-97 1.098815365488779 1102 98-99 1.3402596091757815 1102 100-101 1.1295797573347173 1103 1 0.14009339251957442 1103 2 0.18963899968980158 1103 3 0.1285992005578258 1103 4 0.08018324625499673 1103 5 0.04598755160833434 1103 6 0.06366396810389574 1103 7 0.07687079727091373 1103 8 0.0865765464919761 1103 9 0.12606959321201572 1103 10-11 0.08175694425415969 1103 12-13 0.06527942622893335 1103 14-15 0.03326360462390454 1103 16-17 -0.03594182462651219 1103 18-19 -0.003790416895377291 1103 20-21 -0.10193777166772122 1103 22-23 -0.16631912473423682 1103 24-25 -0.035346867362775924 1103 26-27 -0.13361272896364085 1103 28-29 -0.2344680238826342 1103 30-31 -0.2228980618407732 1103 32-33 -0.18050818555812498 1103 34-35 -0.12563044032606996 1103 36-37 0.007159613534874154 1103 38-39 -0.05266166189347388 1103 40-41 0.11299362703834248 1103 42-43 -0.05184811302401471 1103 44-45 0.06131738775265205 1103 46-47 -0.15790543419569758 1103 48-49 -0.00942086993121194 1103 50-51 0.12059127537553138 1103 52-53 -0.1376835278660451 1103 54-55 -0.07630300030795922 1103 56-57 -0.025619927737736248 1103 58-59 -0.0858511003078064 1103 60-61 0.03362958069797628 1103 62-63 0.11811509994151237 1103 64-65 0.033338376920575286 1103 66-67 0.33272871299100615 1103 68-69 0.3137992878774263 1103 70-71 0.6021478093466612 1103 72-73 0.5529969262595777 1103 74-75 0.5008399206404022 1103 76-77 0.22576532979938335 1103 78-79 0.1913404277544224 1103 80-81 0.1699581728925601 1103 82-83 0.35491513642787 1103 84-85 0.4754099450546434 1103 86-87 0.7308524171454209 1103 88-89 0.70909916499566 1103 90-91 0.5429884606757547 1103 92-93 0.7108720265074631 1103 94-95 0.459728910849595 1103 96-97 0.7430185801024756 1103 98-99 0.8225137109069394 1103 100-101 0.8429702525187714 1104 1 -0.2141159954721239 1104 2 -0.07610478247454822 1104 3 -0.14345617043468195 1104 4 -0.1558841479986839 1104 5 -0.10248969935101115 1104 6 -0.09870901061906068 1104 7 -0.13003052763554024 1104 8 -0.17942304421436006 1104 9 -0.19271277890449312 1104 10-11 -0.07734090601915256 1104 12-13 -0.0712972737421822 1104 14-15 -5.606473763393183E-4 1104 16-17 -0.05623146741909579 1104 18-19 -0.14504490717932583 1104 20-21 -0.12151725358919663 1104 22-23 -0.004921205067994094 1104 24-25 -0.14714662718841964 1104 26-27 -0.03216672248557728 1104 28-29 0.0290832063876465 1104 30-31 -0.05856613506485786 1104 32-33 0.07230713677622447 1104 34-35 0.20313612619869303 1104 36-37 0.2569624981202949 1104 38-39 0.18468126906470417 1104 40-41 0.23114445071588108 1104 42-43 0.18979473877634945 1104 44-45 0.2564232119807599 1104 46-47 0.3510683813977664 1104 48-49 0.4031690915702484 1104 50-51 0.26499298983813446 1104 52-53 0.11701075365736102 1104 54-55 0.33892620414565755 1104 56-57 0.2846358392515924 1104 58-59 0.2683893223242677 1104 60-61 0.24083008473170509 1104 62-63 0.3395051869320085 1104 64-65 0.45867968005343585 1104 66-67 0.39481760139511124 1104 68-69 0.3832100140724286 1104 70-71 0.49392495413896853 1104 72-73 0.6588099870905886 1104 74-75 0.5280337970254045 1104 76-77 0.559339538688743 1104 78-79 0.5970184943924153 1104 80-81 0.5744138123152318 1104 82-83 0.7114632897239304 1104 84-85 0.5734825250798643 1104 86-87 0.5950827476760239 1104 88-89 0.7547396940025344 1104 90-91 0.6883970479477632 1104 92-93 0.9775268506670081 1104 94-95 1.0417420383726181 1104 96-97 0.949568395504798 1104 98-99 1.2519514567168741 1104 100-101 0.9304839129546174 1105 1 -0.06676430855499405 1105 2 -0.1244237377464259 1105 3 0.02963089490276971 1105 4 -0.10300681127240807 1105 5 0.05195249245279143 1105 6 -0.01871907759240088 1105 7 0.08206074625604032 1105 8 -0.05881203486045905 1105 9 -0.009379771815666516 1105 10-11 0.06038605529290919 1105 12-13 0.03742883467352698 1105 14-15 0.01223489519157539 1105 16-17 -0.012952570311945522 1105 18-19 0.0364443057791064 1105 20-21 0.058178639924598485 1105 22-23 0.20487619709360416 1105 24-25 -0.05700485064726735 1105 26-27 0.02798148573299386 1105 28-29 0.01866640572770706 1105 30-31 0.27840118921751866 1105 32-33 0.08111587826473254 1105 34-35 0.1299244820662011 1105 36-37 0.17404824915845296 1105 38-39 0.20705260056174524 1105 40-41 0.08995878496735799 1105 42-43 0.18080493158685584 1105 44-45 -0.002976544734053732 1105 46-47 0.25917369312438865 1105 48-49 0.25369565020337603 1105 50-51 0.03489497666718222 1105 52-53 0.019439830432006033 1105 54-55 0.25622657746258426 1105 56-57 0.2530824493381374 1105 58-59 0.30956380254685456 1105 60-61 0.33794142266042115 1105 62-63 0.22546259804202862 1105 64-65 0.2654202177975975 1105 66-67 0.31045231008206997 1105 68-69 0.2966281709773959 1105 70-71 0.30753801883325593 1105 72-73 0.62758880729724 1105 74-75 0.5071142069749293 1105 76-77 0.4345417869794552 1105 78-79 0.5211200869316706 1105 80-81 0.6101497500118533 1105 82-83 0.6458494140790165 1105 84-85 0.7110356912827989 1105 86-87 0.7718292976403731 1105 88-89 0.7233977105291451 1105 90-91 1.0577811449262704 1105 92-93 1.2828236166518217 1105 94-95 1.272797672382211 1105 96-97 1.2705131529886131 1105 98-99 1.3218600150424038 1105 100-101 1.09394848979219 1106 1 -0.1768715146057147 1106 2 -0.837702716339912 1106 3 -0.31345205968210976 1106 4 0.029823914189897494 1106 5 -0.08102048052018063 1106 6 -0.17560697961508964 1106 7 -0.14523134686403694 1106 8 -0.16745305071585648 1106 9 -0.12455098924618824 1106 10-11 -0.6407054341426104 1106 12-13 -0.5187450542346355 1106 14-15 -0.4474309966309207 1106 16-17 -0.2806485075842673 1106 18-19 -0.08906379539009635 1106 20-21 -0.23247951502111164 1106 22-23 -0.2618156809204706 1106 24-25 -0.1780415227494032 1106 26-27 -0.16168232441862074 1106 28-29 0.0030368737567059156 1106 30-31 0.013244196538060748 1106 32-33 -0.04097862959357457 1106 34-35 0.08142016259910889 1106 36-37 -0.09945103960399138 1106 38-39 0.06525577164040186 1106 40-41 0.22779558196975103 1106 42-43 -0.07797347454712877 1106 44-45 0.03345755303509179 1106 46-47 0.028288636051911453 1106 48-49 0.05531657793469691 1106 50-51 -0.28091461983561317 1106 52-53 -0.24805279698359328 1106 54-55 0.06458726163841533 1106 56-57 0.004877543533744699 1106 58-59 -0.14293663113457455 1106 60-61 -0.13095494482387693 1106 62-63 0.1509637603082723 1106 64-65 7.420094223391516E-4 1106 66-67 0.17341140524912646 1106 68-69 0.2006183871242193 1106 70-71 0.10067336213547762 1106 72-73 -0.02137694921665556 1106 74-75 -0.016930132614980664 1106 76-77 0.1587491907889138 1106 78-79 0.20462076347471125 1106 80-81 0.2977756043642792 1106 82-83 0.12339373632166684 1106 84-85 0.1059036786539913 1106 86-87 0.07476722915340162 1106 88-89 0.4813663466877678 1106 90-91 0.44018894425319743 1106 92-93 0.7761730251103458 1106 94-95 0.6757557267138203 1106 96-97 0.45635362760957676 1106 98-99 0.5976956324312894 1106 100-101 0.3485906158720553 1107 1 0.07271803805064181 1107 2 0.09480886970535707 1107 3 0.036404143867798666 1107 4 0.06901903773974283 1107 5 -0.04988723020884578 1107 6 0.001126770746601835 1107 7 0.07811675244903427 1107 8 0.04078003541653885 1107 9 0.11913963177223508 1107 10-11 0.060792638713188296 1107 12-13 0.07407519460755907 1107 14-15 0.06804250853344485 1107 16-17 0.1563416304891021 1107 18-19 0.17684213823173422 1107 20-21 0.08940512169902348 1107 22-23 0.14484052007163228 1107 24-25 0.06327114140955103 1107 26-27 0.08852269942488533 1107 28-29 0.006013516225500837 1107 30-31 0.053077571702118576 1107 32-33 0.13900992424097325 1107 34-35 0.22652069222404947 1107 36-37 0.1627564717022878 1107 38-39 0.17393119771431742 1107 40-41 0.05267764330265834 1107 42-43 -0.1942124020606144 1107 44-45 -0.011198078845993109 1107 46-47 0.17103798581059948 1107 48-49 0.19542060055846378 1107 50-51 -0.0621333966625599 1107 52-53 0.10888212453184565 1107 54-55 0.1921900253438409 1107 56-57 0.1547747518523579 1107 58-59 0.24012184502269918 1107 60-61 0.22522222774944822 1107 62-63 0.10832719364635324 1107 64-65 0.0605805961086574 1107 66-67 0.180971309779153 1107 68-69 0.502081370129229 1107 70-71 0.23009296481285801 1107 72-73 0.45230086680382087 1107 74-75 0.4596583825328011 1107 76-77 0.5506792444867763 1107 78-79 0.5012463028113103 1107 80-81 0.435326620531189 1107 82-83 0.4686530894854286 1107 84-85 0.3865501472082151 1107 86-87 0.30864428198960425 1107 88-89 0.2232213448733269 1107 90-91 0.25183317952625117 1107 92-93 0.4890745001629817 1107 94-95 0.30427490303752336 1107 96-97 0.2490517413902147 1107 98-99 0.6142725895097669 1107 100-101 0.5925298233050285 1108 1 0.15614066830347184 1108 2 0.13552826499627102 1108 3 0.11167420671352701 1108 4 0.1962309465699974 1108 5 0.09128395922611787 1108 6 0.11142053347744252 1108 7 0.03221330896674601 1108 8 0.01845097868162071 1108 9 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-0.11437227795733662 2316 18-19 0.08364590426475615 2316 20-21 0.2077611407133233 2316 22-23 0.04372044990673629 2316 24-25 0.045359225491132804 2316 26-27 -0.1345161480875987 2316 28-29 -0.1272658689594337 2316 30-31 -0.024255241532301852 2316 32-33 -0.14280777301300418 2316 34-35 -0.15702156904327325 2316 36-37 0.008935198364468988 2316 38-39 0.17160407810933265 2316 40-41 -0.1728227903823054 2316 42-43 0.074572940841243 2316 44-45 -0.016214529800826938 2316 46-47 -0.2874821066442479 2316 48-49 -0.1903615587565568 2316 50-51 -0.1374564798476996 2316 52-53 -0.03485294483352419 2316 54-55 -0.20896008780189845 2316 56-57 -0.0036038478539950347 2316 58-59 -0.15324616695572502 2316 60-61 0.0024171824669210196 2316 62-63 0.2734182190326706 2316 64-65 0.4976938533008024 2316 66-67 0.2520665702566447 2316 68-69 0.3462842862366209 2316 70-71 0.339357347889937 2316 72-73 0.4994344272362454 2316 74-75 0.3894898511015441 2316 76-77 0.1102321855492292 2316 78-79 0.4543223163984891 2316 80-81 0.4376573743677241 2316 82-83 0.6636041727287179 2316 84-85 0.7931673560214669 2316 86-87 1.3314454507584728 2316 88-89 1.031967486530828 2316 90-91 1.1952687882115818 2316 92-93 1.0672625692230717 2316 94-95 1.551263336489896 2316 96-97 1.8078293797211806 2316 98-99 2.25568901914378 2316 100-101 2.7826013449820053 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5195.0 3 881.0 4 852.0 5 874.0 6 1131.0 7 1325.0 8 1612.0 9 2087.0 10 2680.0 11 3459.0 12 4599.0 13 6010.0 14 6708.0 15 8378.0 16 10475.0 17 13811.0 18 12145.0 19 9136.0 20 9441.0 21 10185.0 22 11588.0 23 12886.0 24 14486.0 25 16809.0 26 19356.0 27 22830.0 28 27034.0 29 32300.0 30 38790.0 31 47869.0 32 59190.0 33 76487.0 34 99807.0 35 134298.0 36 180401.0 37 218835.0 38 141301.0 39 12686.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.560237156694726 19.50850907983234 15.07893577817433 22.85231798529861 2 14.533673796086457 20.416465255849285 41.07368183159335 23.976179116470906 3 18.79245768650386 24.779633964422842 30.451984804885917 25.975923544187385 4 11.85704656329034 16.526009261024832 38.79955083794148 32.81739333774335 5 12.904921799410051 37.78597890220198 35.01374425099521 14.295355047392752 6 30.768156687522357 36.97866004807059 18.048591456488587 14.204591807918469 7 27.596537166004858 32.96979747316037 21.384396224017372 18.0492691368174 8 25.755203348719284 34.69409047692264 20.393628270822024 19.15707790353605 9 25.736134037118163 16.714549768089924 21.05101707787032 36.49829911692159 10-11 24.130879185155226 26.889971711573384 28.21271359017441 20.76643551309698 12-13 25.237700548498992 24.273914505078018 28.588043639855478 21.900341306567515 14-15 22.068826754115026 25.552445286923266 27.29839242647627 25.080335532485442 16-17 21.27456798044955 29.063764481543203 27.681215635811274 21.980451902195973 18-19 20.99107814767742 27.764760506833053 30.07398733584023 21.170174009649294 20-21 22.00536882746612 26.525654877338567 30.09676831097629 21.37220798421902 22-23 21.476232399544315 26.663673208483907 30.084803755286753 21.77529063668503 24-25 21.499559594371906 26.73748451913431 30.120019357454858 21.642936529038927 26-27 21.31595624198047 26.87315884085797 30.144618246730225 21.666266670431337 28-29 21.0413476503665 27.329433489808213 30.102835688824182 21.526383171001104 30-31 21.28302665433857 26.79940420313739 30.083269554929387 21.834299587594654 32-33 21.06034652170144 26.880084788810514 30.130780150412274 21.92878853907577 34-35 21.590298895893795 26.823752109741466 29.936657745903776 21.649291248460962 36-37 21.494598809008362 26.616984494518803 30.027633453185494 21.860783243287337 38-39 21.229789401574536 27.0791923328637 30.004285923232178 21.68673234232959 40-41 21.483798160662456 27.041375291832303 29.896802921734437 21.5780236257708 42-43 21.265205133263187 26.97473095782192 29.61909172945069 22.140972179464203 44-45 21.391276930666745 26.9455688567805 29.52826185678756 22.134892355765196 46-47 21.511496105733894 27.320067177953334 29.157184958225574 22.0112517580872 48-49 21.58594057969025 27.322555908788708 29.00545800785655 22.086045503664494 50-51 21.394509146714622 27.46254381918095 29.112765543451996 22.03018149065243 52-53 21.659973352291185 27.6931762719259 28.607680754798707 22.039169620984207 54-55 21.459765660546687 27.384305093148992 28.813664362391467 22.342264883912854 56-57 21.630005540635445 27.59059997670816 28.655750869817027 22.123643612839373 58-59 22.002138368182774 27.29109056385248 28.652486941016093 22.05428412694865 60-61 21.632001242121078 27.303099499858064 29.18894355406599 21.87595570395487 62-63 21.374790219113198 27.414989726618256 29.228986621076903 21.981233433191647 64-65 21.29531791497305 27.61311565652189 29.01734516521996 22.074221263285093 66-67 21.341319008782936 27.462358845671268 28.655936323713927 22.540385821831872 68-69 21.27214831771007 27.379906311376068 28.945016846621442 22.402928524292424 70-71 21.517188106543912 27.33299089130341 28.783638067529044 22.366182934623634 72-73 21.447051119676587 27.117974818550046 29.00505432715495 22.429919734618416 74-75 21.2805253195902 27.63456785476071 28.632599788488754 22.452307037160338 76-77 21.375531020374865 27.253973632524485 28.629881151835747 22.7406141952649 78-79 21.411082668804347 27.315817979520755 28.58936834678471 22.683731004890188 80-81 21.480629210945192 27.350433383489193 28.549193722007914 22.6197436835577 82-83 21.4754679456816 27.206606605664597 28.835829252761247 22.482096195892556 84-85 21.405350347638514 27.21417151316651 28.744572926459966 22.63590521273501 86-87 21.357964088948 27.182492695618627 28.79412230584774 22.665420909585638 88-89 21.365274542227024 27.142956293694642 28.474955366718333 23.01681379736 90-91 21.387948247007767 26.97794898418519 28.714393938205713 22.919708830601333 92-93 21.441889737843578 26.94339060346366 28.793876546695447 22.82084311199732 94-95 21.29305014172781 26.90413051584689 28.58686646452139 23.215952877903913 96-97 21.105251558589554 26.86793497569863 28.82157374997157 23.205239715740245 98-99 21.241825195261946 26.880067829898174 28.434045463601038 23.444061511238836 100-101 21.505911782984338 25.94534614954821 29.203051268126952 23.3456907993405 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1987.0 1 1115.0 2 256.0 3 247.5 4 198.5 5 194.5 6 318.0 7 660.0 8 1100.0 9 1330.5 10 1280.5 11 1240.5 12 1347.0 13 1473.5 14 1607.0 15 1693.0 16 1753.0 17 1905.0 18 2023.0 19 2140.5 20 2378.0 21 2670.5 22 3167.5 23 3880.5 24 4806.0 25 6072.5 26 7805.0 27 9732.0 28 11466.0 29 13694.0 30 16491.5 31 19081.5 32 21797.5 33 24705.5 34 28037.0 35 31131.0 36 34241.0 37 37150.5 38 39235.0 39 40610.0 40 41426.0 41 43004.5 42 44315.0 43 45604.0 44 47461.5 45 48857.0 46 49661.0 47 52188.5 48 56593.5 49 55726.5 50 49682.5 51 44774.5 52 41390.5 53 38897.0 54 36630.5 55 33258.0 56 29075.0 57 25275.0 58 22381.5 59 19807.0 60 16921.0 61 13755.5 62 10504.0 63 7660.5 64 5747.0 65 4472.5 66 3287.0 67 2424.0 68 1763.5 69 1190.0 70 831.5 71 608.0 72 475.5 73 369.0 74 263.0 75 192.5 76 138.5 77 88.5 78 58.5 79 42.5 80 27.0 81 22.5 82 18.0 83 11.0 84 6.5 85 4.5 86 4.0 87 3.5 88 2.5 89 2.5 90 2.0 91 0.5 92 0.5 93 1.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3271676716710826 2 0.2812343008815764 3 0.28021703713325685 4 0.27896502021224817 5 0.2806865434786351 6 0.24617782709333316 7 0.24602132497820706 8 0.2489948651656027 9 0.25869799630342 10-11 0.2651537085523711 12-13 0.2564678411628733 14-15 0.24480843358597992 16-17 0.24120888493807993 18-19 0.22868871572799304 20-21 0.23361853235446478 22-23 0.2297059794763126 24-25 0.2329533983651789 26-27 0.22896259442946373 28-29 0.24930786939585486 30-31 0.2615541599044711 32-33 0.2536508030906037 34-35 0.25012950550026686 36-37 0.23741370864627234 38-39 0.22172437160488226 40-41 0.2194942164643355 42-43 0.23463579610278432 44-45 0.2309188708685398 46-47 0.2437520443088788 48-49 0.2508337650183342 50-51 0.2646450766782113 52-53 0.27638273531266777 54-55 0.2508728905471157 56-57 0.2201593504536214 58-59 0.20865644499185407 60-61 0.211277855420216 62-63 0.21894645906139423 64-65 0.2099867129704258 66-67 0.21425139560761164 68-69 0.21569904017252794 70-71 0.2177726931979486 72-73 0.218555203773579 74-75 0.22133311631706704 76-77 0.23741370864627234 78-79 0.23768758734774303 80-81 0.22790620515236262 82-83 0.21832045060088986 84-85 0.20493951975760952 86-87 0.20939983003870297 88-89 0.22102011208681485 90-91 0.21941596540677247 92-93 0.22227212900782353 94-95 0.21773356766916704 96-97 0.2274758243357659 98-99 0.23350115576812022 100-101 0.21366451267588882 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1277938.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.01202013834245 #Duplication Level Percentage of deduplicated Percentage of total 1 81.38127783642668 51.279987179129606 2 11.15221388894688 14.054470523148463 3 3.254645734804799 6.15245407754071 4 1.415733498455126 3.5683291086072155 5 0.7036776453890357 2.217007498107766 6 0.44492486762477523 1.682136883129301 7 0.2905869177131199 1.281732809761458 8 0.20573812906688146 1.0371180105589786 9 0.14989890217720175 0.8500889378434736 >10 0.9140994283148206 10.485048584724037 >50 0.06165811126164784 2.6546842147950582 >100 0.023312966176342438 2.4848636388823624 >500 0.00111603682133084 0.47820139569364806 >1k 9.92032730071858E-4 1.3670934018065468 >5k 1.2400409125898224E-4 0.4067837362714268 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5206 0.4073750056732017 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3596 0.2813908029967025 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2722 0.21299937868660296 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2559 0.20024445630382695 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2214 0.17324784144457714 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2135 0.16706600789709675 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1691 0.13232253833910565 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1491 0.11667232682649704 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 7.825105756304296E-5 0.0 0.0 0.0 0.0 7 2.347531726891289E-4 0.0 0.0 0.0 0.0 8 3.1300423025217185E-4 0.0 0.0 0.0 0.0 9 3.1300423025217185E-4 0.0 0.0 7.825105756304296E-5 0.0 10-11 4.695063453782578E-4 0.0 0.0 7.825105756304296E-5 0.0 12-13 5.477574029413007E-4 0.0 0.0 7.825105756304296E-5 0.0 14-15 5.477574029413007E-4 0.0 0.0 7.825105756304296E-5 0.0 16-17 5.477574029413007E-4 0.0 0.0 1.1737658634456444E-4 0.0 18-19 6.260084605043437E-4 0.0 0.0 1.956276439076074E-4 0.0 20-21 7.042595180673867E-4 0.0 0.0 2.347531726891289E-4 0.0 22-23 7.825105756304296E-4 0.0 0.0 3.1300423025217185E-4 0.0 24-25 0.0010172637483195585 0.0 0.0 4.303808165967363E-4 0.0 26-27 0.0010172637483195585 0.0 0.0 6.260084605043437E-4 0.0 28-29 0.0015650211512608593 0.0 0.0 0.001565021151260859 0.0 30-31 0.0016432722088239023 0.0 0.0 0.0034430465327738905 0.0 32-33 0.0017215232663869452 0.0 0.0 0.006260084605043437 0.0 34-35 0.0017997743239499882 0.0 0.0 0.008607616331934726 0.0 36-37 0.0018780253815130312 0.0 0.0 0.01216803945105318 0.0 38-39 0.002034527496639117 0.0 0.0 0.02007139626492052 0.0 40-41 0.0020736530254206386 0.0 0.0 0.03579985883509215 0.0 42-43 0.0022301551405467246 0.0 0.0 0.05062843424328879 0.0 44-45 0.002425782784454332 0.0 0.0 0.06964344123110824 0.0 46-47 0.0029735401873956326 0.0 0.0 0.10536504900863736 0.0 48-49 0.0031300423025217186 0.0 0.0 0.13455269347965237 0.0 50-51 0.0031300423025217186 0.0 0.0 0.17532149446999776 0.0 52-53 0.0031300423025217186 0.0 0.0 0.2241892799181181 0.0 54-55 0.0032082933600847616 0.0 0.0 0.2836992091948123 0.0 56-57 0.0034430465327738905 0.0 0.0 0.39544171939483763 0.0 58-59 0.0034430465327738905 0.0 0.0 0.4833567825669164 0.0 60-61 0.0034430465327738905 0.0 0.0 0.5744801390990799 0.0 62-63 0.0034430465327738905 0.0 0.0 0.6786322967154901 0.0 64-65 0.0035212975903369335 0.0 0.0 0.7851319860587915 0.0 66-67 0.0035212975903369335 0.0 0.0 0.9404212097926503 0.0 68-69 0.0036777997054630194 0.0 0.0 1.1340925772611818 0.0 70-71 0.0036777997054630194 0.0 0.0 1.290164311570671 0.0 72-73 0.0037560507630260624 0.0 0.0 1.479688372988361 0.0 74-75 0.0038734273493706266 0.0 0.0 1.732400163388208 0.0 76-77 0.003951678406933669 0.0 0.0 2.0077656349525563 0.0 78-79 0.004029929464496713 0.0 0.0 2.323665154334561 0.0 80-81 0.004538561338656492 0.0 0.0 2.670943347799345 0.0 82-83 0.004538561338656492 0.0 0.0 3.043692260500901 0.0 84-85 0.004812440040127142 0.0 0.0 3.4445333028675886 0.0 86-87 0.005086318741597793 0.0 0.0 3.810435248032377 0.0 88-89 0.005086318741597793 0.0 0.0 4.305647065820095 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 5515 0.0 28.274231 1 GTATCAA 7595 0.0 27.729269 1 TACATGG 5510 0.0 27.424166 2 ACATGGG 5490 0.0 26.051638 3 GTATAGG 350 0.0 24.449327 1 GACGCGA 100 0.0016516153 23.755257 8 ATCAACG 8925 0.0 23.26559 3 TATCAAC 8990 0.0 23.09828 2 CAACGCA 9010 0.0 23.046104 5 GGTATCA 5495 0.0 23.013168 1 TCAACGC 9025 0.0 22.95605 4 AACGCAG 9210 0.0 22.487877 6 CATGGGG 2600 0.0 21.748611 4 GAGTACT 4295 0.0 21.400425 12-13 AGTACTT 4540 0.0 21.134893 12-13 AGAGTAC 7395 0.0 20.33252 10-11 ACTACGT 165 6.3126485E-5 20.155977 8 CGCAGAG 10180 0.0 20.068293 8 ACGCAGA 10275 0.0 19.975224 7 GTCTAGG 405 0.0 19.955214 1 >>END_MODULE