FastQCFastQC Report
Thu 26 May 2016
SRR937347_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937347_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1277938
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55030.43061556976942544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42600.33334950521856305No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41720.3264634121530152No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32310.25282916698619184No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT14320.11205551443027753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG54750.024.0404681
TACATGG55300.022.8472442
ACATGGG54600.021.6612763
GTATTAG3600.021.1185841
GAGTACT50400.021.01854512-13
AGTACTT51900.020.8687212-13
AGAGTAC79700.020.23530610-11
TGAACCG2151.0793738E-619.8829775
GTACTTT53000.019.85067214-15
ATACCGT1456.148107E-419.6544366
GTATCAA105250.019.6387771
GGTATCA85050.018.9955521
GAGTACA45800.018.5709821
CATGGGG26550.017.8901024
AGTACAT42500.017.6581632
TACTTTT60850.017.6410814-15
GGACCGT1350.009402988517.5919346
CAACGCA122250.016.8623545
ATCAACG121500.016.8491633
CCGCTCC1700.001786755616.7660479
Produced by FastQC (version 0.11.5)