##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937347_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1277938 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13721401194737 33.0 31.0 34.0 31.0 34.0 2 32.35445616297504 34.0 31.0 34.0 31.0 34.0 3 32.42526789249557 34.0 31.0 34.0 31.0 34.0 4 36.068558099062706 37.0 35.0 37.0 35.0 37.0 5 35.9925567594046 37.0 35.0 37.0 35.0 37.0 6 36.03470277900806 37.0 35.0 37.0 35.0 37.0 7 35.970245817872225 37.0 35.0 37.0 35.0 37.0 8 35.939170757892796 37.0 35.0 37.0 35.0 37.0 9 37.62345043343261 39.0 37.0 39.0 35.0 39.0 10-11 37.53069241230796 39.0 37.0 39.0 35.0 39.0 12-13 37.418680327214624 39.0 37.0 39.0 34.0 39.0 14-15 38.61150736577205 40.0 38.0 41.0 34.0 41.0 16-17 38.56664994702403 40.0 38.0 41.0 34.0 41.0 18-19 38.54384837136074 40.0 38.0 41.0 34.0 41.0 20-21 38.42794329615364 40.0 38.0 41.0 34.0 41.0 22-23 38.37102504190344 40.0 38.0 41.0 34.0 41.0 24-25 38.40952534473503 40.0 38.0 41.0 34.0 41.0 26-27 38.24317846405694 40.0 38.0 41.0 34.0 41.0 28-29 38.09303933367659 40.0 38.0 41.0 33.0 41.0 30-31 38.169608384757325 40.0 38.0 41.0 34.0 41.0 32-33 38.082880781383764 40.0 37.0 41.0 33.5 41.0 34-35 37.946525966048426 40.0 37.0 41.0 33.0 41.0 36-37 37.786179376464275 40.0 37.0 41.0 33.0 41.0 38-39 37.67179511056092 40.0 37.0 41.0 33.0 41.0 40-41 37.487096400607854 40.0 37.0 41.0 33.0 41.0 42-43 37.2865412876055 40.0 36.5 41.0 32.0 41.0 44-45 36.976969539993334 39.0 36.0 41.0 31.0 41.0 46-47 36.62739819928666 39.0 36.0 41.0 30.5 41.0 48-49 36.30191057782146 39.0 36.0 40.0 30.0 41.0 50-51 36.050443761747445 39.0 35.0 40.0 29.5 41.0 52-53 35.80902907652797 39.0 35.0 40.0 29.0 41.0 54-55 35.58920111930313 38.5 35.0 40.0 28.0 41.0 56-57 35.274750026996614 38.0 34.5 40.0 27.5 41.0 58-59 35.007127888833416 38.0 34.0 40.0 27.0 41.0 60-61 34.62824291945306 38.0 34.0 40.0 26.0 41.0 62-63 34.24594894274996 37.0 33.5 40.0 26.0 41.0 64-65 33.819768251667924 36.5 33.0 39.0 25.5 40.5 66-67 33.35991926055881 36.0 32.5 39.0 23.5 40.0 68-69 32.88356986019666 35.5 32.0 39.0 22.5 40.0 70-71 32.41328726432738 35.0 31.0 38.0 22.0 40.0 72-73 31.905326001730913 35.0 31.0 37.5 20.5 39.5 74-75 31.403539530086746 34.5 30.5 37.0 19.5 39.0 76-77 30.10694963292429 33.5 29.5 35.5 18.5 38.0 78-79 30.77191264364938 34.0 30.0 36.0 18.5 38.0 80-81 30.573386971824924 34.0 30.0 36.0 18.0 37.0 82-83 30.13882246243558 34.0 30.0 35.0 15.0 37.0 84-85 29.662404201142778 34.0 29.0 35.0 8.5 36.5 86-87 29.25221724371605 34.0 29.0 35.0 6.0 36.0 88-89 28.75529955287346 33.5 29.0 35.0 2.0 35.5 90-91 28.1344998740158 33.0 27.5 35.0 2.0 35.0 92-93 27.549326727900727 33.0 26.5 35.0 2.0 35.0 94-95 26.966015174445083 32.0 25.5 34.0 2.0 35.0 96-97 26.1856255937299 32.0 25.0 34.0 2.0 35.0 98-99 25.0843405548626 32.0 20.5 34.0 2.0 35.0 100-101 22.06907885985079 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.07693715644181509 1101 2 0.17428129304042983 1101 3 0.13683945623898808 1101 4 0.08415445712854819 1101 5 0.1057913834150952 1101 6 0.1237603430459373 1101 7 0.1347728061290283 1101 8 0.07093489107852946 1101 9 0.12316477727127051 1101 10-11 0.04435401292626295 1101 12-13 0.10742814599327488 1101 14-15 0.24121676264269354 1101 16-17 0.17269333025326716 1101 18-19 0.16378060126422866 1101 20-21 0.23744608820696556 1101 22-23 0.12445349457681232 1101 24-25 0.06010701350752612 1101 26-27 0.023837333675714945 1101 28-29 -0.09238246039828368 1101 30-31 0.05199938417883487 1101 32-33 -0.016533529256456347 1101 34-35 -0.039576203179287006 1101 36-37 0.07835853669659087 1101 38-39 0.09061826898831526 1101 40-41 0.051946166186567666 1101 42-43 0.024456582923029657 1101 44-45 0.05501896041759835 1101 46-47 0.12857422021297538 1101 48-49 0.05081890029289582 1101 50-51 0.1372008148457411 1101 52-53 -0.03472843911080048 1101 54-55 -0.12505188828320257 1101 56-57 -0.032601641712645346 1101 58-59 0.10922409719254489 1101 60-61 0.08446987657600857 1101 62-63 0.10506399677611 1101 64-65 0.16557505512923143 1101 66-67 -0.012597468755188856 1101 68-69 0.13380848301158466 1101 70-71 0.11451466112416853 1101 72-73 0.15833690809660084 1101 74-75 0.14605444190684125 1101 76-77 0.2543092064085499 1101 78-79 0.3379234918960954 1101 80-81 0.2430712441417704 1101 82-83 0.3614867765062044 1101 84-85 0.5140005018483897 1101 86-87 0.5959512736684012 1101 88-89 0.8799881037994552 1101 90-91 0.7925929813442742 1101 92-93 0.8940494315051524 1101 94-95 1.038868334588134 1101 96-97 1.2968905827473094 1101 98-99 1.6252342583171142 1101 100-101 2.4160929902785497 1102 1 0.13509687326103403 1102 2 0.16551384638176359 1102 3 0.06666599533136974 1102 4 0.006300489796693398 1102 5 0.07353404022898502 1102 6 0.05843488916482187 1102 7 0.05398006592157145 1102 8 0.01235238562941987 1102 9 0.09758629569104471 1102 10-11 0.0317700574550841 1102 12-13 -0.014878103740180393 1102 14-15 0.04579044116888298 1102 16-17 0.1355691919963249 1102 18-19 0.1726735705013951 1102 20-21 0.08488198120962664 1102 22-23 0.058373060999471704 1102 24-25 0.1302657206134441 1102 26-27 0.1117499358429086 1102 28-29 0.0769069250482417 1102 30-31 0.06264943898690234 1102 32-33 0.12142339194786445 1102 34-35 0.13074410416084703 1102 36-37 0.1161304590593204 1102 38-39 0.0775619613346592 1102 40-41 0.169007208888452 1102 42-43 0.1979596771871499 1102 44-45 0.005177160361583333 1102 46-47 0.05230559497913845 1102 48-49 0.19099811287788526 1102 50-51 0.21055372001914208 1102 52-53 0.1917093361542257 1102 54-55 0.28384603730922464 1102 56-57 0.353049248759973 1102 58-59 0.3110385842731702 1102 60-61 0.4589165225643015 1102 62-63 0.4312496895074389 1102 64-65 0.522531299733437 1102 66-67 0.3989205245433567 1102 68-69 0.4695092251969797 1102 70-71 0.4384691871410027 1102 72-73 0.6104755753903035 1102 74-75 0.38168383562932817 1102 76-77 0.2828341835574477 1102 78-79 0.3660446989875972 1102 80-81 0.5876812077990046 1102 82-83 0.5376273433518755 1102 84-85 0.6539488404896332 1102 86-87 0.6400035897271081 1102 88-89 0.6882447000743497 1102 90-91 0.8660940008550213 1102 92-93 0.9097203894533585 1102 94-95 0.9584127418022845 1102 96-97 1.2569416677856857 1102 98-99 1.7018393393881013 1102 100-101 2.359116459197015 1103 1 0.12292678872983487 1103 2 0.1811541746377472 1103 3 0.10599836136302798 1103 4 0.07662077630596542 1103 5 0.04817296648496239 1103 6 0.0461150099404648 1103 7 0.0770903030671306 1103 8 0.11643106015198157 1103 9 0.0555759823753732 1103 10-11 0.07598763963166277 1103 12-13 0.06034477705653529 1103 14-15 0.10071895581253898 1103 16-17 0.2284639303842937 1103 18-19 0.13264791448875712 1103 20-21 0.14676065142437977 1103 22-23 0.18338994961683852 1103 24-25 0.24453337142146125 1103 26-27 0.2285387635085243 1103 28-29 0.20302291998851274 1103 30-31 0.14013490498546588 1103 32-33 0.20767821132287878 1103 34-35 0.20622508708816412 1103 36-37 0.22610056865421058 1103 38-39 0.2727432227038875 1103 40-41 0.32642172800832725 1103 42-43 0.35397109487153244 1103 44-45 0.3332040411661197 1103 46-47 0.32945967693347455 1103 48-49 0.3416143516016703 1103 50-51 0.1718233475296671 1103 52-53 0.09830084113072957 1103 54-55 0.15207149888248495 1103 56-57 0.30832304996185655 1103 58-59 0.3982265609482809 1103 60-61 0.3823785324762099 1103 62-63 0.4877066240226853 1103 64-65 0.4702188074434517 1103 66-67 0.48405616706468635 1103 68-69 0.3963252166126594 1103 70-71 0.5240118698170946 1103 72-73 0.4868217695281487 1103 74-75 0.547021074394241 1103 76-77 0.2962785020307521 1103 78-79 0.42455298860634016 1103 80-81 0.5891139255055222 1103 82-83 0.5963756226710046 1103 84-85 0.5965509162720437 1103 86-87 0.6271715448239448 1103 88-89 0.645811681799394 1103 90-91 1.1289134404858032 1103 92-93 1.108931299402272 1103 94-95 1.0654804568359921 1103 96-97 1.4536972420084666 1103 98-99 1.6491650880189006 1103 100-101 2.3849909224326957 1104 1 -0.006902928483562221 1104 2 0.02858216907799971 1104 3 0.05512959919588667 1104 4 -0.04586918353339087 1104 5 -0.022192042438469173 1104 6 0.022696452108519338 1104 7 -0.07945439616435124 1104 8 -0.10474567991744976 1104 9 -0.047974536305666504 1104 10-11 -0.05686789009198634 1104 12-13 0.007464346903432784 1104 14-15 -0.09888870919825621 1104 16-17 -0.02372070053462494 1104 18-19 -0.07898820578125054 1104 20-21 -0.010552771953562967 1104 22-23 -0.08282337756669733 1104 24-25 -0.19035387544494853 1104 26-27 -0.06114882803717592 1104 28-29 -0.1693703000722877 1104 30-31 -0.11624238898088635 1104 32-33 -0.047671693209892396 1104 34-35 -0.09625215244420104 1104 36-37 -0.05012876669027122 1104 38-39 -0.042147223717087456 1104 40-41 0.03250407602428851 1104 42-43 0.11676437871565071 1104 44-45 -0.058010065129728616 1104 46-47 0.15948710688089562 1104 48-49 0.13386158679178806 1104 50-51 0.2119495793062356 1104 52-53 0.10317366922532045 1104 54-55 0.21918501242968347 1104 56-57 0.19464822650870417 1104 58-59 0.19689316216950914 1104 60-61 0.30039228432789855 1104 62-63 0.39215213498531654 1104 64-65 0.18553545426872375 1104 66-67 0.25855859997273 1104 68-69 0.369113420644517 1104 70-71 0.255231757126289 1104 72-73 0.4308911146790564 1104 74-75 0.28504832566522964 1104 76-77 0.299679586970619 1104 78-79 0.31720715415627865 1104 80-81 0.3187768867140406 1104 82-83 0.17319544723568825 1104 84-85 0.21464551401115983 1104 86-87 0.4562876730720653 1104 88-89 0.5940630421294912 1104 90-91 0.7383509317857175 1104 92-93 0.7357194920071457 1104 94-95 0.7215052284522017 1104 96-97 0.8119722977409651 1104 98-99 0.8741858383767713 1104 100-101 1.7005980223273625 1105 1 -0.07556555903962447 1105 2 -0.0520467371173865 1105 3 -0.03075622454818472 1105 4 0.011968654304446602 1105 5 -0.0368129230189993 1105 6 -0.10362448859421391 1105 7 -0.2133020772650056 1105 8 -0.19311469041786467 1105 9 -0.08611130732396788 1105 10-11 -0.11109420124535774 1105 12-13 -0.11810632464757731 1105 14-15 -0.02886885488972979 1105 16-17 -0.033007902894766517 1105 18-19 -0.056625123609578054 1105 20-21 -0.003173680497909004 1105 22-23 -0.07719943494845438 1105 24-25 -0.06290557354761006 1105 26-27 -0.16336859565787165 1105 28-29 -0.023296140035370172 1105 30-31 -0.16373571262008824 1105 32-33 -0.02719099523307733 1105 34-35 -0.10393311328088117 1105 36-37 -0.13254428101473792 1105 38-39 -0.1283242905925448 1105 40-41 -0.08846403124491076 1105 42-43 -0.13295444512629473 1105 44-45 -0.09610237368246288 1105 46-47 0.03725545463888835 1105 48-49 0.1107006969832014 1105 50-51 0.09101335078594275 1105 52-53 -0.12027888317188484 1105 54-55 -0.013547192097654204 1105 56-57 -0.02295177908406032 1105 58-59 -0.12296170563156039 1105 60-61 0.09308785625782434 1105 62-63 -0.004747516865194257 1105 64-65 -0.09423006914397547 1105 66-67 -0.004069579163470394 1105 68-69 0.04509977430287648 1105 70-71 0.09753108180016312 1105 72-73 0.1899808925065436 1105 74-75 0.015941350091623008 1105 76-77 0.28568803204269244 1105 78-79 0.08993614284491613 1105 80-81 0.19927212587456822 1105 82-83 0.23250907540084498 1105 84-85 0.29117128965747696 1105 86-87 0.4946682885556264 1105 88-89 0.614173778836193 1105 90-91 0.6881031723863771 1105 92-93 0.5870722415492402 1105 94-95 0.7244737272148036 1105 96-97 1.0489696831242803 1105 98-99 1.026252084654086 1105 100-101 1.3793860705929077 1106 1 -0.11823426111782354 1106 2 -0.016187180513284716 1106 3 -0.07442551643313777 1106 4 0.022166759983939244 1106 5 -0.0913665780861379 1106 6 -0.050514125342644434 1106 7 -0.09194205228495633 1106 8 -0.08030131221295989 1106 9 -0.06827693893760767 1106 10-11 -0.18939417002029302 1106 12-13 -0.41631539379374516 1106 14-15 -0.12108063020804849 1106 16-17 -0.15820340648565434 1106 18-19 0.05105485418592792 1106 20-21 0.18974065874505897 1106 22-23 0.04908894585854284 1106 24-25 0.1081734258625815 1106 26-27 0.15293886713226357 1106 28-29 0.07321324483088176 1106 30-31 0.0076034956510753204 1106 32-33 0.1808494585711813 1106 34-35 -0.3352917504803301 1106 36-37 0.14479395581002308 1106 38-39 0.2059091341030168 1106 40-41 -0.005114622786386747 1106 42-43 0.05025236887640716 1106 44-45 0.1572872804132288 1106 46-47 0.43885972900312 1106 48-49 0.5221384376763893 1106 50-51 0.4750726090172108 1106 52-53 0.51506094960056 1106 54-55 0.5178381598781954 1106 56-57 0.3471297530257118 1106 58-59 0.6449899302148125 1106 60-61 0.5994477077653002 1106 62-63 0.5156296125106579 1106 64-65 0.5226966907352875 1106 66-67 0.4322691086899795 1106 68-69 0.5533705996853868 1106 70-71 0.39547300318239564 1106 72-73 0.5403458788022135 1106 74-75 0.7793299633518664 1106 76-77 0.6469262792758066 1106 78-79 0.6886923444894357 1106 80-81 0.6078451404809577 1106 82-83 0.6058928314290135 1106 84-85 0.7273646074939251 1106 86-87 0.9749094154334692 1106 88-89 1.1819175945389304 1106 90-91 1.0104762771985065 1106 92-93 1.1978153980561963 1106 94-95 0.7359305498911368 1106 96-97 1.215667459205882 1106 98-99 1.2292205473401374 1106 100-101 1.8960463765985267 1107 1 -0.08513709820020665 1107 2 -0.007700871949602117 1107 3 0.00361623009824541 1107 4 -0.0346612134213089 1107 5 -0.12742919308732326 1107 6 -0.13357635827210856 1107 7 -0.030820168207753795 1107 8 -0.055628910740303184 1107 9 0.012380160482571512 1107 10-11 -0.16747500296862228 1107 12-13 -0.023241123207036196 1107 14-15 -0.10971399641980639 1107 16-17 0.02461991171934841 1107 18-19 -0.04492507985474248 1107 20-21 0.07258411964345868 1107 22-23 -0.04352268223060207 1107 24-25 -0.1455493755064836 1107 26-27 -0.11740885010908642 1107 28-29 -0.17662049801726454 1107 30-31 -0.18269939610431862 1107 32-33 -0.11246412639661685 1107 34-35 -0.18004633733930575 1107 36-37 -0.13192868409776537 1107 38-39 -0.06748756345444917 1107 40-41 -0.1016776558283965 1107 42-43 -0.012872993811974709 1107 44-45 -0.05316709718553625 1107 46-47 -0.0401710877191519 1107 48-49 -0.029000423813812404 1107 50-51 0.15078683535394077 1107 52-53 0.1681849854289723 1107 54-55 0.18294030411586704 1107 56-57 -0.0355474507208271 1107 58-59 0.14707094220931793 1107 60-61 0.3556983372244673 1107 62-63 0.24596131151487555 1107 64-65 0.3690666896322341 1107 66-67 0.24578333726478263 1107 68-69 0.3454592371280967 1107 70-71 0.5551498088709437 1107 72-73 0.5420807881680787 1107 74-75 0.49151605306358803 1107 76-77 0.49571260206726464 1107 78-79 0.5108627356893187 1107 80-81 0.47414001992054366 1107 82-83 0.7010486735294847 1107 84-85 0.5674847762449318 1107 86-87 0.7269620518082966 1107 88-89 0.9659815268083243 1107 90-91 0.873398854120417 1107 92-93 0.933767068381055 1107 94-95 1.096719360145368 1107 96-97 1.3231395192119244 1107 98-99 1.522991339475535 1107 100-101 2.126732171363667 1108 1 0.01887209214221741 1108 2 -0.027659940172448216 1108 3 -0.03392709097486346 1108 4 -0.020499204675040517 1108 5 -0.10174064143509298 1108 6 -0.022071053552409126 1108 7 -0.12988665338446737 1108 8 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0.2705597924305465 2316 92-93 0.5117067982849655 2316 94-95 0.36889059544490266 2316 96-97 0.5411970466746041 2316 98-99 0.587565577134896 2316 100-101 1.0444507461634167 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 4.0 4 9.0 5 22.0 6 52.0 7 111.0 8 303.0 9 565.0 10 944.0 11 1579.0 12 2480.0 13 3490.0 14 4446.0 15 6438.0 16 8852.0 17 12526.0 18 11429.0 19 7268.0 20 6698.0 21 6894.0 22 7361.0 23 8353.0 24 9665.0 25 11580.0 26 13999.0 27 17092.0 28 21820.0 29 27236.0 30 34006.0 31 42525.0 32 54133.0 33 71433.0 34 97113.0 35 137505.0 36 201028.0 37 269023.0 38 169643.0 39 10310.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.351495224675126 20.517692187255363 15.370831376232974 21.759981211836543 2 15.58150708406824 21.881656230583957 39.33391134781187 23.202925337535937 3 19.561512373839733 25.26225841942254 30.492637357993896 24.683591848743834 4 12.842485316189048 17.65547311371913 37.92116675456869 31.58087481552313 5 13.174304948561225 38.54116862700295 34.42434505257342 13.860181371862398 6 31.159727623718837 37.819988137139674 17.226657318273656 13.793626920867835 7 27.997915391826524 33.488322594679865 20.824797447137495 17.688964566356113 8 26.283278218505124 35.48865437916394 19.725057084146492 18.50301031818445 9 26.168327414945015 17.01506645862319 20.667825825666032 36.148780300765765 10-11 24.600215346910414 27.52664839765309 27.527235280584815 20.345900974851673 12-13 25.74074182762945 24.745090904110985 28.12727553737408 21.386891730885484 14-15 22.33586449420864 26.25111703384671 26.875756100843702 24.537262371100947 16-17 21.564739447453633 29.730745936031326 27.20092837054693 21.503586245968116 18-19 21.40033397551368 28.310880496549913 29.647134681025218 20.641650846911197 20-21 22.418154252457573 26.959417029393062 29.883503692473223 20.73892502567614 22-23 21.95222819412182 27.07508512793546 29.81302959200801 21.15965708593471 24-25 22.013987382012033 27.1589964297941 29.859871863843107 20.967144324350762 26-27 21.798585274221967 27.331501965505545 29.96962631723303 20.900286443039466 28-29 21.390403205895943 27.885065719380027 29.837729297896427 20.886801776827603 30-31 21.745564040153795 27.291770756997707 29.835167866322 21.127497336526503 32-33 21.55912224155025 27.406984851365912 29.80405137342451 21.22984153365933 34-35 22.011709488253732 27.294242756690856 29.74420511793217 20.94984263712324 36-37 22.013822266807935 27.14513536650448 29.69874125348804 21.142301113199544 38-39 21.690058516140844 27.549615083047847 29.812792169886176 20.94753423092513 40-41 21.900358233341525 27.574694546996803 29.51019533028989 21.014751889371787 42-43 21.803746637319637 27.629953533871358 29.129234531670335 21.437065297138666 44-45 21.989290560261924 27.444563038269465 29.181423511938764 21.384722889529854 46-47 22.147435947596833 27.90405324827965 28.736566249692864 21.211944554430655 48-49 22.213636342295164 27.961763403232393 28.50580388093945 21.31879637353299 50-51 21.95274731637998 28.133602725640838 28.59990077765901 21.313749180320173 52-53 22.387471066671466 28.394961257901404 28.01149195031371 21.206075725113426 54-55 22.062690052256055 28.096589975413515 28.221439537755355 21.61928043457507 56-57 22.184057442536336 28.259078296443175 28.21361443199905 21.34324982902144 58-59 22.644643167352406 27.904209750394777 28.12679488363285 21.324352198619962 60-61 22.2705248611435 27.995450483513284 28.65103784377646 21.08298681156676 62-63 21.90751820510854 28.10054165382045 28.672283005904823 21.31965713516618 64-65 21.90047560992787 28.246910256992123 28.40482089115434 21.447793241925662 66-67 22.033424156727477 28.07706633655154 28.160677591557647 21.728831915163333 68-69 21.9419095449075 28.130785687568565 28.254891864863552 21.67241290266038 70-71 22.162381899591374 28.074679679295865 28.196399199335183 21.566539221777585 72-73 22.17125029295274 27.733146365253674 28.42007408036315 21.67552926143044 74-75 21.900084354640054 28.44304653277389 27.910391583942257 21.746477528643798 76-77 21.996059276741125 27.92353776161285 28.08273953822486 21.99766342342117 78-79 22.0854611100069 28.077614093954477 27.986529862951098 21.85039493308752 80-81 22.10979718890901 28.077496717368135 28.005036238064758 21.807669855658098 82-83 22.01355626625183 28.00017684808202 28.14001203505974 21.84625485060641 84-85 21.926676325466875 27.98738434969187 28.16000638529379 21.925932939547465 86-87 21.870466329352443 28.025733642790186 28.08172227447654 22.022077753380838 88-89 21.784507542619437 27.979917648587023 27.80013584383593 22.435438964957612 90-91 21.963497603119094 27.8359047320545 28.029713419050537 22.170884245775866 92-93 21.9701804144787 27.801303447755082 28.030465166597086 22.19805097116913 94-95 21.76111047640809 27.79962721196177 27.966106336927144 22.473155974702998 96-97 21.56364393264775 27.716994095175195 28.213066674596103 22.506295297580948 98-99 21.721731434592307 27.852483832309087 27.66198111092548 22.763803622173125 100-101 21.641199702762595 27.555660599960945 28.105252407227766 22.69788729004869 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 212.0 1 149.0 2 103.5 3 148.5 4 149.0 5 138.5 6 270.0 7 664.5 8 1113.0 9 1364.5 10 1378.5 11 1356.0 12 1442.5 13 1569.5 14 1696.5 15 1833.5 16 1938.0 17 2000.5 18 2114.0 19 2302.0 20 2548.5 21 2853.5 22 3315.0 23 3956.5 24 4849.5 25 6034.5 26 7550.5 27 9555.5 28 11513.0 29 13796.0 30 16605.0 31 19079.0 32 21779.5 33 24685.5 34 28197.0 35 31355.5 36 34335.0 37 37252.0 38 39582.0 39 41210.0 40 41735.5 41 43021.0 42 44706.0 43 46261.5 44 48327.0 45 49762.0 46 49916.0 47 52486.0 48 57666.5 49 56250.0 50 50025.0 51 45327.5 52 41642.0 53 38907.5 54 36397.5 55 33085.0 56 28578.5 57 24414.5 58 21603.0 59 19363.0 60 16352.0 61 12879.0 62 9839.5 63 7384.5 64 5477.0 65 4220.5 66 3034.0 67 2239.5 68 1594.0 69 997.5 70 717.5 71 514.0 72 386.0 73 278.0 74 206.5 75 162.5 76 111.0 77 66.5 78 38.0 79 22.0 80 16.5 81 12.0 82 6.5 83 6.5 84 4.0 85 1.5 86 2.5 87 2.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04209906896891712 2 0.0 3 0.0 4 0.0 5 8.607616331934726E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0062992101338249585 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.912552878152148E-5 22-23 6.651339892858652E-4 24-25 3.912552878152148E-5 26-27 0.0017606487951684667 28-29 0.003325669946429326 30-31 1.1737658634456444E-4 32-33 7.825105756304296E-5 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.009820507724161891 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.1737658634456444E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.912552878152148E-4 84-85 1.1737658634456444E-4 86-87 0.0 88-89 0.0 90-91 0.01029001406954015 92-93 0.001525895622479338 94-95 0.0 96-97 0.0 98-99 2.738787014706504E-4 100-101 0.01342005637206187 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1277938.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.71887982025469 #Duplication Level Percentage of deduplicated Percentage of total 1 80.67720129275587 49.793064908220956 2 11.443902649722904 14.12609704625884 3 3.4606707493898146 6.4076616623718206 4 1.4312210608139215 3.533334425943674 5 0.753656434109773 2.3257415441291385 6 0.47226923705154844 1.748875697063274 7 0.32367907783233957 1.398397707354552 8 0.2289423852897569 1.1304054050768761 9 0.17110642064183101 0.9504446950860403 >10 0.9439355958291179 10.707995875576852 >50 0.06388668449294514 2.7007279528219716 >100 0.02737399272252199 2.8909014308225447 >500 8.871138490302511E-4 0.4127493980095049 >1k 0.0011405749487531796 1.443175884531623 >5k 1.267305498614644E-4 0.43042636673229207 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5503 0.43061556976942544 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4260 0.33334950521856305 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4172 0.3264634121530152 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3231 0.25282916698619184 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1432 0.11205551443027753 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 7.825105756304296E-5 0.0 0.0 0.0 0.0 6 3.912552878152148E-4 0.0 0.0 0.0 0.0 7 3.912552878152148E-4 0.0 0.0 0.0 0.0 8 3.912552878152148E-4 0.0 0.0 0.0 0.0 9 3.912552878152148E-4 0.0 0.0 0.0 0.0 10-11 4.303808165967363E-4 0.0 0.0 0.0 0.0 12-13 6.260084605043437E-4 0.0 0.0 0.0 0.0 14-15 6.260084605043437E-4 0.0 0.0 0.0 0.0 16-17 7.433850468489082E-4 0.0 0.0 1.1737658634456445E-4 0.0 18-19 0.0010172637483195585 0.0 0.0 2.7387870147065036E-4 0.0 20-21 0.0011737658634456444 7.825105756304296E-5 0.0 3.1300423025217185E-4 0.0 22-23 0.0012520169210086874 2.3475317268912887E-4 0.0 3.912552878152148E-4 0.0 24-25 0.0014867700936978163 3.1300423025217185E-4 0.0 5.086318741597792E-4 0.0 26-27 0.0015650211512608593 3.1300423025217185E-4 0.0 7.042595180673867E-4 0.0 28-29 0.0017215232663869452 3.1300423025217185E-4 0.0 0.0016041466800423808 0.0 30-31 0.0017997743239499882 3.1300423025217185E-4 0.0 0.0034430465327738905 0.0 32-33 0.0017997743239499882 3.1300423025217185E-4 0.0 0.006260084605043437 0.0 34-35 0.002034527496639117 3.1300423025217185E-4 0.0 0.008646741860716248 0.0 36-37 0.002269280669328246 3.1300423025217185E-4 0.0 0.012481043681305353 0.0 38-39 0.0025822848995804178 3.1300423025217185E-4 0.0 0.020227898380046606 0.0 40-41 0.0026605359571434607 3.1300423025217185E-4 0.0 0.036269365180470414 0.0 42-43 0.0028170380722695467 3.1300423025217185E-4 0.0 0.051371819290137705 0.0 44-45 0.0032865444176478045 3.1300423025217185E-4 0.0 0.07058245392186475 0.0 46-47 0.003951678406933669 3.1300423025217185E-4 0.0 0.10696919568867973 0.0 48-49 0.004382059223530406 3.1300423025217185E-4 0.0 0.1367828486201991 0.0 50-51 0.004382059223530406 3.1300423025217185E-4 0.0 0.17806028148470426 0.0 52-53 0.004421184752311928 3.1300423025217185E-4 0.0 0.22806270726748873 0.0 54-55 0.004460310281093449 3.1300423025217185E-4 0.0 0.28769001313052744 0.0 56-57 0.004616812396219535 3.1300423025217185E-4 0.0 0.400723665780343 0.0 58-59 0.004695063453782578 3.1300423025217185E-4 0.0 0.4896951182295229 0.0 60-61 0.004695063453782578 3.1300423025217185E-4 0.0 0.5808967258192494 0.0 62-63 0.004695063453782578 3.1300423025217185E-4 0.0 0.6852836366083488 0.0 64-65 0.004695063453782578 3.1300423025217185E-4 0.0 0.7917442004228687 0.0 66-67 0.004929816626471707 3.1300423025217185E-4 0.0 0.9475420560308873 0.0 68-69 0.00500806768403475 3.1300423025217185E-4 0.0 1.1430523233521501 0.0 70-71 0.00500806768403475 3.1300423025217185E-4 0.0 1.3001413214099589 0.0 72-73 0.005086318741597793 3.1300423025217185E-4 0.0 1.4919346634969772 0.0 74-75 0.0051645697991608355 3.1300423025217185E-4 0.0 1.7491067641779179 0.0 76-77 0.0051645697991608355 3.1300423025217185E-4 0.0 2.028071784390166 0.0 78-79 0.0051645697991608355 3.1300423025217185E-4 0.0 2.349253250157676 0.0 80-81 0.005242820856723879 3.1300423025217185E-4 0.0 2.7022046452957813 0.0 82-83 0.0053210719142869215 3.1300423025217185E-4 0.0 3.0792573661633034 0.0 84-85 0.005594950615757572 3.1300423025217185E-4 0.0 3.4859672378472197 0.0 86-87 0.005907954846009744 3.1300423025217185E-4 0.0 3.8588726526639006 0.0 88-89 0.006025331432354309 3.1300423025217185E-4 0.0 4.3610096890459475 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 5475 0.0 24.040468 1 TACATGG 5530 0.0 22.847244 2 ACATGGG 5460 0.0 21.661276 3 GTATTAG 360 0.0 21.118584 1 GAGTACT 5040 0.0 21.018545 12-13 AGTACTT 5190 0.0 20.86872 12-13 AGAGTAC 7970 0.0 20.235306 10-11 TGAACCG 215 1.0793738E-6 19.882977 5 GTACTTT 5300 0.0 19.850672 14-15 ATACCGT 145 6.148107E-4 19.654436 6 GTATCAA 10525 0.0 19.638777 1 GGTATCA 8505 0.0 18.995552 1 GAGTACA 4580 0.0 18.570982 1 CATGGGG 2655 0.0 17.890102 4 AGTACAT 4250 0.0 17.658163 2 TACTTTT 6085 0.0 17.64108 14-15 GGACCGT 135 0.0094029885 17.591934 6 CAACGCA 12225 0.0 16.862354 5 ATCAACG 12150 0.0 16.849163 3 CCGCTCC 170 0.0017867556 16.766047 9 >>END_MODULE