FastQCFastQC Report
Thu 26 May 2016
SRR937346_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937346_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1256179
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52250.41594390608344833No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36430.2900064401649765No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27100.2157335857389751No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24510.19511550503550848No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21440.1706763128503183No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18820.14981941267924395No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT16690.13286323047909573No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC13350.10627466308543608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA74350.027.7677671
GTACATG52400.025.0558991
AATAGCG1351.3329942E-524.6530635
TACATGG52000.024.414582
GGTATCA52600.024.0562571
ACATGGG53450.023.0396583
ATCAACG90950.022.5842153
TCAACGC91050.022.507194
AGTACTT45750.022.42696412-13
TATCAAC92000.022.3273542
CAACGCA91800.022.2706245
AGAGTAC73500.022.10276610-11
AACGCAG93250.021.9050776
GTATAGA3550.021.4399531
GAGTACT43900.021.09978112-13
CATGGGG24450.020.2245314
CGCGGAA958.282386E-619.99307482-83
GTACTTT46500.019.81706414-15
ACGCAGA102100.019.772937
CGCAGAG102350.019.678228
Produced by FastQC (version 0.11.5)