##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937346_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1256179 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.793056562798775 33.0 31.0 34.0 30.0 34.0 2 31.91364367657794 33.0 31.0 34.0 30.0 34.0 3 31.95417054416608 33.0 31.0 34.0 30.0 34.0 4 35.56844605744882 37.0 35.0 37.0 33.0 37.0 5 35.56966801705808 37.0 35.0 37.0 33.0 37.0 6 35.61306390251708 37.0 35.0 37.0 33.0 37.0 7 35.559558788994245 37.0 35.0 37.0 33.0 37.0 8 35.55487872349403 37.0 35.0 37.0 33.0 37.0 9 37.19148942945233 39.0 37.0 39.0 34.0 39.0 10-11 37.07222338536148 39.0 37.0 39.0 33.0 39.0 12-13 36.943400980274305 39.0 37.0 39.0 33.0 39.0 14-15 38.137347464015875 40.0 38.0 41.0 33.0 41.0 16-17 38.11170103942193 40.0 38.0 41.0 33.0 41.0 18-19 38.08775381534001 40.0 38.0 41.0 33.5 41.0 20-21 38.029756109599035 40.0 38.0 41.0 33.0 41.0 22-23 37.88240250792283 40.0 38.0 41.0 33.0 41.0 24-25 37.72741942032147 40.0 37.5 41.0 32.5 41.0 26-27 37.5813056897146 40.0 37.0 41.0 32.0 41.0 28-29 37.63285526983017 40.0 37.0 41.0 33.0 41.0 30-31 37.48968379506424 40.0 37.0 41.0 32.0 41.0 32-33 37.298810519838334 40.0 37.0 41.0 32.0 41.0 34-35 37.19254461346671 39.5 36.5 41.0 31.5 41.0 36-37 37.213830194582144 40.0 37.0 41.0 31.5 41.0 38-39 37.14616189253283 40.0 37.0 41.0 31.0 41.0 40-41 36.980521884221915 40.0 36.5 41.0 31.0 41.0 42-43 36.754094360755914 39.5 36.0 41.0 31.0 41.0 44-45 36.538489339497 39.0 36.0 41.0 30.0 41.0 46-47 36.20128261975403 39.0 36.0 41.0 29.5 41.0 48-49 35.899132209661204 39.0 35.0 41.0 28.5 41.0 50-51 35.031374907556966 38.0 34.0 40.0 27.0 40.5 52-53 35.132189361548 38.5 35.0 40.0 27.5 40.5 54-55 35.46833373269256 39.0 35.0 40.0 27.5 41.0 56-57 35.2815991988403 39.0 35.0 40.0 26.5 41.0 58-59 35.054984202092214 38.5 35.0 40.0 26.0 41.0 60-61 34.78193593428962 38.0 34.0 40.0 26.0 41.0 62-63 34.46372849729219 38.0 34.0 40.0 25.5 41.0 64-65 34.11121703196758 37.5 34.0 40.0 24.5 41.0 66-67 33.74560114442289 37.0 33.5 39.5 23.0 41.0 68-69 33.27853673720067 36.0 33.0 39.0 22.0 41.0 70-71 32.83177437291978 36.0 32.0 39.0 21.5 40.0 72-73 32.43748900435368 35.0 32.0 38.5 20.5 40.0 74-75 31.977961341496712 35.0 31.5 37.5 18.5 39.5 76-77 31.54092290987192 35.0 31.0 37.0 18.0 39.0 78-79 31.05784685144394 35.0 31.0 36.5 14.0 39.0 80-81 30.55673116649777 34.5 30.5 36.0 8.5 38.5 82-83 30.059524160171442 34.0 30.0 35.5 7.0 37.0 84-85 29.61436347845331 34.0 29.5 35.0 2.0 37.0 86-87 29.20052237778215 34.0 29.0 35.0 2.0 36.0 88-89 28.790233716691652 34.0 29.0 35.0 2.0 36.0 90-91 28.362128327252726 34.0 29.0 35.0 2.0 35.5 92-93 27.901445972269876 34.0 27.5 35.0 2.0 35.0 94-95 27.367796707316394 33.0 26.5 35.0 2.0 35.0 96-97 26.743312457858316 33.0 25.5 35.0 2.0 35.0 98-99 25.975972771396435 32.5 24.5 34.5 2.0 35.0 100-101 24.07250678446304 30.5 11.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.11016580625092587 1101 2 -0.1684624827489074 1101 3 -0.07542399672157529 1101 4 -0.06501348255252282 1101 5 -0.024682130748828968 1101 6 -0.041370361398811895 1101 7 -0.13894491790649965 1101 8 -0.07014868317428125 1101 9 -0.095827884959931 1101 10-11 -0.13262880668004584 1101 12-13 -0.10669749669071393 1101 14-15 -0.09661830068522903 1101 16-17 0.10634565834360643 1101 18-19 -2.1307752490429266E-4 1101 20-21 -0.1368668558667423 1101 22-23 0.01764776374059096 1101 24-25 0.021755368483916016 1101 26-27 0.023177307662102464 1101 28-29 -0.16488154975584735 1101 30-31 -0.07387879012108556 1101 32-33 -0.06685874049018281 1101 34-35 -0.10628759135560273 1101 36-37 0.005633671505130167 1101 38-39 0.008540245014856396 1101 40-41 0.030932651866727667 1101 42-43 0.12779749110942618 1101 44-45 0.10660893956390538 1101 46-47 0.31622690018419064 1101 48-49 0.23600923019090203 1101 50-51 0.1464708780831927 1101 52-53 0.13976417317819312 1101 54-55 0.20497214338505643 1101 56-57 0.16774946727588258 1101 58-59 0.3471159444745666 1101 60-61 0.16104580918145928 1101 62-63 0.14567735517761804 1101 64-65 0.04431359625543507 1101 66-67 0.09188187770769218 1101 68-69 0.10879152443843765 1101 70-71 0.18366632268116945 1101 72-73 0.10331822132074109 1101 74-75 0.03279282387790161 1101 76-77 0.10396799893705477 1101 78-79 0.015076105640957849 1101 80-81 0.03625339697160612 1101 82-83 -0.018483597129712592 1101 84-85 0.037500598967561416 1101 86-87 0.1196645267546863 1101 88-89 0.16701177829019187 1101 90-91 0.3032496134453133 1101 92-93 0.09829621108274367 1101 94-95 0.18128930875306182 1101 96-97 0.11318479411599469 1101 98-99 0.12849624153407646 1101 100-101 0.616246772170971 1102 1 0.009484671606241335 1102 2 -0.0068283384399911995 1102 3 0.10385774821366667 1102 4 0.010238384796608102 1102 5 0.0869838122241049 1102 6 -0.11995670244716194 1102 7 -0.054199285288532906 1102 8 -0.04347969191562839 1102 9 -0.06403558067522397 1102 10-11 0.10040934733075346 1102 12-13 0.021950906577743012 1102 14-15 0.02439524335262888 1102 16-17 0.08156364366797675 1102 18-19 0.09182058958474926 1102 20-21 0.11130208933867891 1102 22-23 0.07498897875657917 1102 24-25 -0.01590653057615299 1102 26-27 0.16763216891028065 1102 28-29 0.0937813931776077 1102 30-31 0.09980518379084913 1102 32-33 0.09077370642797433 1102 34-35 0.06631764029217635 1102 36-37 0.14216002930808713 1102 38-39 0.29357085945276395 1102 40-41 0.414595156197926 1102 42-43 0.4382253695464229 1102 44-45 0.17864585233029828 1102 46-47 0.2608460179796026 1102 48-49 0.2344315778533712 1102 50-51 0.26717158905746174 1102 52-53 0.20160742111566776 1102 54-55 0.2841660582172665 1102 56-57 0.43005565990121397 1102 58-59 0.5113939832206924 1102 60-61 0.41506241904912855 1102 62-63 0.23013559366321346 1102 64-65 0.30580475622684133 1102 66-67 0.2525587725791141 1102 68-69 0.2595467975341421 1102 70-71 0.314883290448293 1102 72-73 0.3475888696187397 1102 74-75 0.5198744521449363 1102 76-77 0.48212790893587254 1102 78-79 0.4225823447581405 1102 80-81 0.3817277891785231 1102 82-83 0.48962846136228677 1102 84-85 0.522554130373674 1102 86-87 0.4187108957858534 1102 88-89 0.4558689781656291 1102 90-91 0.6722155428215757 1102 92-93 0.8116553780893234 1102 94-95 0.7805678080315595 1102 96-97 0.9177393496583939 1102 98-99 1.2042228958321601 1102 100-101 1.4338023759727747 1103 1 -0.042047476997343125 1103 2 -0.18896323403455995 1103 3 -0.06324734936024257 1103 4 -0.11560004970617399 1103 5 -0.07643001621480039 1103 6 -0.12208683395396491 1103 7 0.005128214284496835 1103 8 0.0020373464397778207 1103 9 -0.05097119357960622 1103 10-11 0.1302854585766937 1103 12-13 0.054838617927963185 1103 14-15 -0.07161865340150086 1103 16-17 -0.07098817702254223 1103 18-19 -0.03698551204312395 1103 20-21 -0.14309053441523645 1103 22-23 -0.3977933608599571 1103 24-25 -0.2457728664533292 1103 26-27 -0.014084520677940304 1103 28-29 0.0059631880701545015 1103 30-31 -0.1036969825207592 1103 32-33 -0.04942485715461942 1103 34-35 -0.14210063762492808 1103 36-37 -0.05902054310154625 1103 38-39 -0.10902714142813608 1103 40-41 0.06961857213874367 1103 42-43 0.014469313312211796 1103 44-45 0.19572050019230147 1103 46-47 0.08054585797815861 1103 48-49 -0.021624431638848307 1103 50-51 -0.0073341368367039195 1103 52-53 0.003890812427052026 1103 54-55 -0.10999995675814489 1103 56-57 -0.10531420859197738 1103 58-59 0.06528109681272554 1103 60-61 0.11556040105346455 1103 62-63 -0.05820587457469628 1103 64-65 0.05006683948151647 1103 66-67 0.08996618747722351 1103 68-69 0.141598546313908 1103 70-71 0.16143505720574325 1103 72-73 0.22641575795032054 1103 74-75 0.2315343322337533 1103 76-77 0.34898940881452134 1103 78-79 0.40180215796994645 1103 80-81 0.173527041306631 1103 82-83 0.23859541836864295 1103 84-85 0.34323285557570316 1103 86-87 0.4490327413754791 1103 88-89 0.3949749476574915 1103 90-91 0.29787509931196965 1103 92-93 0.4800979034153947 1103 94-95 0.2477150408156774 1103 96-97 0.2920242870510883 1103 98-99 0.41941617846840984 1103 100-101 0.6429063903908805 1104 1 -0.11211042916552927 1104 2 -0.2040558499153704 1104 3 -0.08232718247412052 1104 4 -0.08876585580461693 1104 5 -0.024642426151032737 1104 6 -0.14544423360776193 1104 7 -0.09201859520197075 1104 8 -0.13057276437379528 1104 9 -0.148676509376088 1104 10-11 -0.1369579614052583 1104 12-13 -0.06785060844833168 1104 14-15 -0.04216299362430931 1104 16-17 0.04260128015371123 1104 18-19 0.07099576504997884 1104 20-21 0.08500288778594012 1104 22-23 -0.0075768379501042205 1104 24-25 0.0311094107729204 1104 26-27 -0.024094174330052454 1104 28-29 -0.02263621865559884 1104 30-31 -0.13991204813600433 1104 32-33 -0.20214674320453696 1104 34-35 -0.10657815682126426 1104 36-37 0.11270072896129335 1104 38-39 0.06729718429570397 1104 40-41 -0.007009603210050841 1104 42-43 0.15033706937440883 1104 44-45 0.08533043346744762 1104 46-47 0.03836013798585469 1104 48-49 0.23598937789200392 1104 50-51 0.1387208113072802 1104 52-53 0.12642839139466133 1104 54-55 0.167669222570467 1104 56-57 0.13837393036142487 1104 58-59 0.12236987342499361 1104 60-61 0.2190736276664822 1104 62-63 0.1379417264372691 1104 64-65 0.18026168790808583 1104 66-67 0.1752863484453968 1104 68-69 0.17270013528285233 1104 70-71 0.1920521142103766 1104 72-73 0.3429706617096926 1104 74-75 0.25758717210888094 1104 76-77 0.23041315605897594 1104 78-79 0.2748520092815099 1104 80-81 0.37515244261745906 1104 82-83 0.3643305384290052 1104 84-85 0.5221240910952254 1104 86-87 0.5779580050940218 1104 88-89 0.5296600388678563 1104 90-91 0.4809958180467113 1104 92-93 0.5736504843803196 1104 94-95 0.6241205533839178 1104 96-97 0.6321333622238186 1104 98-99 0.7516775002420175 1104 100-101 1.065206511708812 1105 1 -0.09178183679346219 1105 2 -0.044849926848986144 1105 3 0.0638780719776868 1105 4 -0.023821808825751134 1105 5 -0.0405378687350364 1105 6 -0.09244707164150157 1105 7 -0.09662452153986578 1105 8 0.08482413096450614 1105 9 0.04351439951035019 1105 10-11 0.029119024349370193 1105 12-13 0.026751836799903117 1105 14-15 0.048982898280954146 1105 16-17 -0.07075253248290636 1105 18-19 -0.0887413947897997 1105 20-21 -0.1454609661330153 1105 22-23 -0.06348219218292428 1105 24-25 -0.0411260110778926 1105 26-27 -0.0042431455428229015 1105 28-29 0.08307414855079998 1105 30-31 -0.2201314845777489 1105 32-33 -0.26200845040042253 1105 34-35 -0.13621214656917857 1105 36-37 -0.23560197146461093 1105 38-39 -0.12027489112893619 1105 40-41 -0.17961567676583456 1105 42-43 -0.013264687834670497 1105 44-45 -0.006081012927865004 1105 46-47 0.09022901285269569 1105 48-49 0.08799910654081344 1105 50-51 0.14914120527870978 1105 52-53 0.14972293860764552 1105 54-55 0.06465797778187721 1105 56-57 0.230554436872751 1105 58-59 0.2822934836983535 1105 60-61 0.27808844862294535 1105 62-63 0.15656108334045626 1105 64-65 0.3124026500587789 1105 66-67 0.4443087654359772 1105 68-69 0.3466302752829691 1105 70-71 0.36765749665995884 1105 72-73 0.41066993236773897 1105 74-75 0.4594419385947326 1105 76-77 0.3041714907384012 1105 78-79 0.3060935110266527 1105 80-81 0.4234548618980938 1105 82-83 0.6530648172964924 1105 84-85 0.4327009807492814 1105 86-87 0.48050321325398215 1105 88-89 0.38174388763419387 1105 90-91 0.48484124642009974 1105 92-93 0.5903039968560115 1105 94-95 0.762514442001315 1105 96-97 0.7675959279862532 1105 98-99 0.7798228257673507 1105 100-101 0.9820480848137372 1106 1 -0.12749294381339382 1106 2 -0.27079278420085373 1106 3 -0.3157450776956203 1106 4 -0.12859044254976482 1106 5 -0.07187896772027358 1106 6 -0.19295725643947037 1106 7 -0.18293804774650368 1106 8 -0.2401033556148704 1106 9 -0.12733441290331626 1106 10-11 -0.6228557242768673 1106 12-13 -0.6371644609495277 1106 14-15 -0.5747228941361868 1106 16-17 -0.5845084411531829 1106 18-19 -0.29750353979713395 1106 20-21 -0.4214786229528187 1106 22-23 -0.3297785090254237 1106 24-25 -0.5205869428341927 1106 26-27 -0.42497902721945735 1106 28-29 -0.26001400265263896 1106 30-31 -0.3936247965177415 1106 32-33 -0.3415090984186335 1106 34-35 -0.39179848417508367 1106 36-37 -0.35169751239217106 1106 38-39 -0.23856275142522776 1106 40-41 -0.41578432853437874 1106 42-43 -0.5856278389466567 1106 44-45 -0.3795592674362567 1106 46-47 -0.11810625092058302 1106 48-49 -0.10350140004727137 1106 50-51 -0.17368890909681056 1106 52-53 -0.35087813943130897 1106 54-55 -0.28774337775274006 1106 56-57 -0.36260503900670216 1106 58-59 -0.3442278045837952 1106 60-61 -0.34965115047141637 1106 62-63 -0.3635495796376418 1106 64-65 -0.2044450613945017 1106 66-67 -0.0643873585250816 1106 68-69 -0.30168923610032294 1106 70-71 -0.12570610800381843 1106 72-73 -0.02049047743824417 1106 74-75 -0.1408003214586948 1106 76-77 0.164591995424491 1106 78-79 0.07096287713926586 1106 80-81 0.1030017795138285 1106 82-83 0.06187865745362231 1106 84-85 -0.226926844652791 1106 86-87 -0.15391667328491465 1106 88-89 0.012274445685772406 1106 90-91 -0.014449012092228486 1106 92-93 0.0450239530126737 1106 94-95 0.2339643019732982 1106 96-97 0.35890385794907687 1106 98-99 0.6218176367447654 1106 100-101 0.47455564096210523 1107 1 0.029457859120249452 1107 2 -0.002804773901829094 1107 3 0.01343454230307728 1107 4 -0.07962235098198533 1107 5 -0.04313709770877949 1107 6 0.005102822117557082 1107 7 -0.04409596840638841 1107 8 -0.07349445147021783 1107 9 -0.00844323196066199 1107 10-11 -0.0686213855297666 1107 12-13 -0.008529053837889933 1107 14-15 -0.09629097020839339 1107 16-17 0.06419304351657473 1107 18-19 -0.008765986410040227 1107 20-21 -0.22292739001697726 1107 22-23 0.11534726800155681 1107 24-25 0.07802302109708847 1107 26-27 0.1555500567292043 1107 28-29 0.04054549827917242 1107 30-31 0.18896177462977448 1107 32-33 0.1793644438559241 1107 34-35 0.12146043581102361 1107 36-37 0.18209287122014928 1107 38-39 0.33444509874363604 1107 40-41 0.17166346037162583 1107 42-43 0.06937183592297202 1107 44-45 0.241700868675764 1107 46-47 0.44079543795870535 1107 48-49 0.2880214483160799 1107 50-51 0.20410980615401542 1107 52-53 0.4319084906547417 1107 54-55 0.49506867476065963 1107 56-57 0.6164451514366789 1107 58-59 0.44714382357221183 1107 60-61 0.5955967233835366 1107 62-63 0.5894267121329548 1107 64-65 0.6924915534034923 1107 66-67 0.660970872876419 1107 68-69 0.6838734192877851 1107 70-71 0.7738453850363172 1107 72-73 0.5104975591479146 1107 74-75 0.5696703981308922 1107 76-77 0.4477021993988295 1107 78-79 0.5817668899397148 1107 80-81 0.4799380345931219 1107 82-83 0.508818419790245 1107 84-85 0.5969630080977453 1107 86-87 0.5012531762004748 1107 88-89 0.5831788061681813 1107 90-91 0.808650497168454 1107 92-93 0.747582417677922 1107 94-95 0.6566163073358773 1107 96-97 0.7584740286909444 1107 98-99 1.0153737604713946 1107 100-101 1.141165236873487 1108 1 -0.028415475976199644 1108 2 -0.11422256975219369 1108 3 -0.1562188359319805 1108 4 -0.07635481114922271 1108 5 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2316 60-61 -1.0703588070839416 2316 62-63 -0.7605913429787137 2316 64-65 -0.8920900729954298 2316 66-67 -0.7766843422837866 2316 68-69 -0.8309815794877906 2316 70-71 -0.7773882425745882 2316 72-73 -0.5779150714057621 2316 74-75 -0.8542573980248243 2316 76-77 -1.0027513920465054 2316 78-79 -1.3211484917702485 2316 80-81 -1.4048288469362937 2316 82-83 -0.9955459365138957 2316 84-85 -0.8037829931446687 2316 86-87 -1.1637774621480261 2316 88-89 -1.485985415103091 2316 90-91 -1.7067343949228508 2316 92-93 -1.4349644460592508 2316 94-95 -2.021546138861968 2316 96-97 -1.896026536482477 2316 98-99 -1.9970918760162526 2316 100-101 -2.6118712731910243 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4828.0 3 907.0 4 753.0 5 892.0 6 957.0 7 1113.0 8 1285.0 9 1734.0 10 2089.0 11 2827.0 12 3753.0 13 4958.0 14 5527.0 15 7084.0 16 9082.0 17 12077.0 18 10324.0 19 7065.0 20 6836.0 21 7262.0 22 7872.0 23 8688.0 24 9892.0 25 11279.0 26 12953.0 27 15465.0 28 18683.0 29 22543.0 30 28092.0 31 35349.0 32 45145.0 33 59519.0 34 82003.0 35 117329.0 36 176707.0 37 258869.0 38 224017.0 39 30417.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.576817511715895 19.467201489406754 15.08383905777444 22.87214194110291 2 14.50395884146585 20.46162790883404 41.021248524518946 24.01316472518117 3 18.795493221385236 24.738250520863694 30.4320154091006 26.034240848650462 4 11.831113695098693 16.60440072962155 38.73215477943643 32.832330795843326 5 12.922367245170067 37.810818128019335 34.95258912948163 14.31422549732897 6 30.838392873528917 36.96282365032161 17.995951303937066 14.202832172212412 7 27.591973110734724 32.959055780226684 21.349457531571193 18.0995135774674 8 25.72364583940045 34.75007998621285 20.38583274623586 19.14044142815084 9 25.717374394160004 16.712368111374833 21.04298222869338 36.527275265771785 10-11 24.170856837792698 26.903133032380495 28.165730478297956 20.76027965152885 12-13 25.237651080181404 24.261753695021497 28.536201795568207 21.964393429228892 14-15 22.097962020100155 25.605216641328383 27.23481867136971 25.062002667201753 16-17 21.286860917852813 29.09307780685517 27.594146933043977 22.025914342248036 18-19 21.048967953586633 27.74578118318345 30.01981758882811 21.185433274401802 20-21 22.075161554223225 26.55137326574269 30.04822793933754 21.325237240696545 22-23 21.513059225221774 26.615530665645544 30.037015763609677 21.834394345523005 24-25 21.476332063040047 26.66299737366365 30.169485441481758 21.691185121814545 26-27 21.303975166328247 26.8605610709142 30.109909657667533 21.725554105090016 28-29 21.050095243794193 27.23146735282091 30.205985897934557 21.51245150545034 30-31 21.29175091724744 26.747358375239187 30.12154426327356 21.839346444239812 32-33 21.026549180952443 26.86685834492592 30.124497782082805 21.982094692038835 34-35 21.596342471568693 26.870577307939065 29.876172601891483 21.656907618600762 36-37 21.422124012053793 26.678606115843234 30.03236813922089 21.86690173288208 38-39 21.1380677306171 27.116656569068965 30.021015500576937 21.724260199736996 40-41 21.48379153323636 26.999779438657423 29.889890832106552 21.626538195999665 42-43 21.192046904547936 27.038242970121445 29.581751294051294 22.187958831279325 44-45 21.367693882419207 26.958529757398164 29.529906586046707 22.143869774135926 46-47 21.56432608563833 27.252338506611007 29.231412422887527 21.95192298486314 48-49 21.599124519401286 27.34480506553138 29.01381961923935 22.042250795827982 50-51 21.412897156139806 27.4388740423447 29.141008832652005 22.007219968863485 52-53 21.727944052193262 27.717853716471303 28.525830844405142 22.028371386930292 54-55 21.45857869186798 27.402638541796325 28.84355141916972 22.29523134716597 56-57 21.596873255164713 27.62319534178831 28.59236659487916 22.187564808167824 58-59 21.92382609971891 27.35809023645512 28.701530848283934 22.016552815542035 60-61 21.605736133549595 27.32910783087196 29.21830065568004 21.846855379898408 62-63 21.440474148761783 27.44187808856786 29.15739680843002 21.960250954240337 64-65 21.338470600762776 27.559623821850987 28.99045197291728 22.111453604468963 66-67 21.346594829133284 27.538394662434047 28.637931653313448 22.477078855119224 68-69 21.27580750389615 27.415213885446025 28.930485036712334 22.378493573945487 70-71 21.436644951529804 27.37515125748111 28.82606645996473 22.362137331024357 72-73 21.478929553265292 27.153857474513693 29.00961860977846 22.357594362442555 74-75 21.293570794963593 27.70275336792194 28.644168118494694 22.359507718619774 76-77 21.287878667009164 27.346431152685753 28.65376668671652 22.711923493588568 78-79 21.39662852511535 27.38840724455478 28.65322884292616 22.561735387403715 80-81 21.480885254088154 27.43133361679248 28.55792418558012 22.529856943539244 82-83 21.418740390808676 27.280750907825546 28.8570670803812 22.44344162098458 84-85 21.4488983038681 27.333397671387804 28.66746849960998 22.550235525134113 86-87 21.262577581108474 27.31253815995537 28.82446001793792 22.600424240998237 88-89 21.301056117561572 27.27177738978403 28.494755304715557 22.932411187938836 90-91 21.271456734759482 27.127207492642526 28.74547374021512 22.855862032382873 92-93 21.438484601708645 27.117798464278565 28.797369157793902 22.64634777621889 94-95 21.209054067095938 27.012850177506486 28.669351397439858 23.108744357957722 96-97 20.99428719554142 27.010219240087334 28.837957747180987 23.157535817190258 98-99 21.163003571692094 27.04462920012843 28.507208197143047 23.28515903103643 100-101 21.07875997395178 26.672188421303233 29.037304543643728 23.21174706110126 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1714.0 1 975.0 2 232.0 3 237.0 4 217.0 5 197.5 6 328.0 7 689.5 8 1165.0 9 1434.0 10 1384.5 11 1319.5 12 1376.0 13 1496.5 14 1651.5 15 1726.5 16 1768.0 17 1843.0 18 1952.5 19 2127.0 20 2413.0 21 2794.0 22 3301.0 23 4008.0 24 4853.0 25 5980.0 26 7521.0 27 9472.5 28 11312.0 29 13302.5 30 15993.5 31 18667.0 32 21277.0 33 24287.0 34 27621.5 35 30548.0 36 33544.5 37 36276.0 38 38216.0 39 39699.5 40 40833.5 41 42191.0 42 43579.0 43 45457.0 44 46975.5 45 48021.5 46 48807.5 47 51262.5 48 56038.5 49 55068.5 50 48834.5 51 43847.0 52 40242.0 53 38166.0 54 36258.5 55 32722.0 56 28559.5 57 24835.5 58 22081.5 59 19587.5 60 16711.5 61 13464.0 62 10116.0 63 7508.5 64 5750.5 65 4381.5 66 3067.5 67 2216.0 68 1584.5 69 1033.5 70 750.5 71 530.0 72 380.5 73 325.0 74 244.5 75 185.0 76 135.0 77 86.5 78 58.0 79 42.5 80 29.5 81 20.0 82 16.0 83 13.5 84 11.5 85 8.0 86 5.0 87 5.5 88 7.0 89 7.0 90 5.5 91 4.5 92 5.5 93 5.5 94 5.5 95 5.0 96 3.5 97 4.0 98 5.5 99 4.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.37208073053283014 2 0.3220082488244112 3 0.3130127155445203 4 0.32025690606195456 5 0.3216102163783983 6 0.2337246522987568 7 0.21828099339345747 8 0.22592321635690454 9 0.21923627126388834 10-11 0.24264057908944506 12-13 0.22855023050058948 14-15 0.2233360054578209 16-17 0.22441069306205563 18-19 0.21867902583947033 20-21 0.21115621260982712 22-23 0.21167365478964384 24-25 0.20152382741631564 26-27 0.20367320262478514 28-29 0.2242116768390492 30-31 0.23667009239925202 32-33 0.22795318183157018 34-35 0.23746615729127776 36-37 0.20948447633657308 38-39 0.2054245453872418 40-41 0.20375280911398772 42-43 0.21131542558823227 44-45 0.2170072895662163 46-47 0.22409226710524535 48-49 0.2328091776729272 50-51 0.23786418973729065 52-53 0.26246259490088597 54-55 0.23173449006869246 56-57 0.19734448673318053 58-59 0.1986181905604217 60-61 0.1944388498772866 62-63 0.20411103831539928 64-65 0.19619019263974322 66-67 0.19296612982703898 68-69 0.18922462483451802 70-71 0.2007675657688912 72-73 0.20025012358907446 74-75 0.20594198756705853 76-77 0.20992231202718722 78-79 0.2072156913942997 80-81 0.2084097887323383 82-83 0.19873760029422557 84-85 0.1898614767481386 86-87 0.19189144222280424 88-89 0.19392140769746985 90-91 0.2008073690134925 92-93 0.19392140769746985 94-95 0.19280691684863382 96-97 0.19236908115801968 98-99 0.20470808698441864 100-101 0.1871150528706498 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1256179.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.38373040634715 #Duplication Level Percentage of deduplicated Percentage of total 1 81.66160937972383 51.76017433472845 2 11.021525707960498 13.971708282799852 3 3.1584039356535323 6.005742707154279 4 1.3767587602325506 3.4905642437262694 5 0.7182372997673045 2.276227968811679 6 0.44203970318510455 1.6810875225351831 7 0.2918664899961796 1.2949710841595268 8 0.20112372755228575 1.0198377700394956 9 0.16401112760604197 0.9356073386240128 >10 0.8773793192762955 10.230610562024141 >50 0.06108514540293916 2.6126891998288255 >100 0.023952893258773898 2.5654735033708613 >500 8.778482932787759E-4 0.3940198884474839 >1k 0.001003255192318601 1.3459630363286756 >5k 1.2540689903982512E-4 0.41532255742144514 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5225 0.41594390608344833 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3643 0.2900064401649765 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2710 0.2157335857389751 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2451 0.19511550503550848 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2144 0.1706763128503183 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1882 0.14981941267924395 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1669 0.13286323047909573 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1335 0.10627466308543608 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.5921297840514766E-4 0.0 0.0 0.0 0.0 3 1.5921297840514766E-4 0.0 0.0 0.0 0.0 4 1.5921297840514766E-4 0.0 0.0 0.0 0.0 5 2.388194676077215E-4 0.0 0.0 0.0 0.0 6 2.388194676077215E-4 0.0 0.0 0.0 0.0 7 2.388194676077215E-4 0.0 0.0 0.0 0.0 8 3.1842595681029533E-4 0.0 0.0 0.0 0.0 9 3.1842595681029533E-4 0.0 0.0 0.0 0.0 10-11 3.5822920141158224E-4 0.0 0.0 0.0 0.0 12-13 4.77638935215443E-4 0.0 0.0 0.0 0.0 14-15 6.368519136205907E-4 0.0 0.0 0.0 0.0 16-17 6.766551582218776E-4 0.0 0.0 0.0 0.0 18-19 9.55277870430886E-4 0.0 0.0 7.960648920257383E-5 0.0 20-21 0.0011940973380386076 3.9803244601286916E-5 0.0 7.960648920257383E-5 0.0 22-23 0.0012737038272411813 1.1940973380386076E-4 0.0 1.990162230064346E-4 0.0 24-25 0.0015921297840514768 1.5921297840514766E-4 0.0 5.1744217981673E-4 0.0 26-27 0.0015921297840514768 1.5921297840514766E-4 0.0 7.164584028231645E-4 0.0 28-29 0.0018309492516591984 1.5921297840514766E-4 0.0 0.00155232653945019 0.0 30-31 0.00206976871926692 1.5921297840514766E-4 0.0 0.0034628822803119616 0.0 32-33 0.00206976871926692 1.5921297840514766E-4 0.0 0.006368519136205907 0.0 34-35 0.002308588186874641 1.5921297840514766E-4 0.0 0.008876123546086983 0.0 36-37 0.0025474076544823626 1.5921297840514766E-4 0.0 0.013174873963025972 0.0 38-39 0.0025474076544823626 1.5921297840514766E-4 0.0 0.01962299958843445 0.0 40-41 0.00258721089908365 1.5921297840514766E-4 0.0 0.03263866057305527 0.0 42-43 0.002786227122090084 1.5921297840514766E-4 0.0 0.0468484188957147 0.0 44-45 0.0031842595681029536 1.5921297840514766E-4 0.0 0.06376479785126164 0.0 46-47 0.0036618985033183967 1.5921297840514766E-4 0.0 0.09393565725903713 0.0 48-49 0.0037415049925209705 1.5921297840514766E-4 0.0 0.1201659954512852 0.0 50-51 0.003821111481723544 1.5921297840514766E-4 0.0 0.15845671675772321 0.0 52-53 0.003860914726324831 1.5921297840514766E-4 0.0 0.20279753124355684 0.0 54-55 0.003900717970926118 1.5921297840514766E-4 0.0 0.25665132118909806 0.0 56-57 0.004298750416938987 2.388194676077215E-4 0.0 0.3558012034909038 0.0 58-59 0.004298750416938987 2.388194676077215E-4 0.0 0.4404229015132397 0.0 60-61 0.004298750416938987 2.388194676077215E-4 0.0 0.5205468328956303 0.0 62-63 0.004298750416938987 2.388194676077215E-4 0.0 0.6206519930678669 0.0 64-65 0.004378356906141561 2.388194676077215E-4 0.0 0.7270858691317081 0.0 66-67 0.004537569884546708 2.388194676077215E-4 0.0 0.8794128862208332 0.0 68-69 0.004696782862951857 2.388194676077215E-4 0.0 1.0624282048975504 0.0 70-71 0.004736586107553144 2.388194676077215E-4 0.0 1.2151532544326882 0.0 72-73 0.004816192596755717 2.388194676077215E-4 0.0 1.3993626704474442 0.0 74-75 0.004895799085958291 2.388194676077215E-4 0.0 1.6410877749110595 0.0 76-77 0.004975405575160865 2.388194676077215E-4 0.0 1.906814235869251 0.0 78-79 0.005055012064363438 2.388194676077215E-4 0.0 2.2181552151405173 0.0 80-81 0.005851076956389177 2.388194676077215E-4 0.0 2.555766335848633 0.0 82-83 0.005890880200990464 2.388194676077215E-4 0.0 2.9312303421725723 7.960648920257383E-5 84-85 0.005890880200990464 2.388194676077215E-4 0.0 3.313819129280142 7.960648920257383E-5 86-87 0.005890880200990464 2.388194676077215E-4 0.0 3.667669973785583 7.960648920257383E-5 88-89 0.005970486690193038 2.388194676077215E-4 0.0 4.15657322722319 7.960648920257383E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7435 0.0 27.767767 1 GTACATG 5240 0.0 25.055899 1 AATAGCG 135 1.3329942E-5 24.653063 5 TACATGG 5200 0.0 24.41458 2 GGTATCA 5260 0.0 24.056257 1 ACATGGG 5345 0.0 23.039658 3 ATCAACG 9095 0.0 22.584215 3 TCAACGC 9105 0.0 22.50719 4 AGTACTT 4575 0.0 22.426964 12-13 TATCAAC 9200 0.0 22.327354 2 CAACGCA 9180 0.0 22.270624 5 AGAGTAC 7350 0.0 22.102766 10-11 AACGCAG 9325 0.0 21.905077 6 GTATAGA 355 0.0 21.439953 1 GAGTACT 4390 0.0 21.099781 12-13 CATGGGG 2445 0.0 20.224531 4 CGCGGAA 95 8.282386E-6 19.993074 82-83 GTACTTT 4650 0.0 19.817064 14-15 ACGCAGA 10210 0.0 19.77293 7 CGCAGAG 10235 0.0 19.67822 8 >>END_MODULE