FastQCFastQC Report
Thu 26 May 2016
SRR937346_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937346_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1256179
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57670.4590906232312433No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43930.3497113070669069No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40980.32622739275214757No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32690.2602336132032139No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT13210.10516017223660004No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCCG908.944313E-426.3907599
GTACCGT908.950552E-426.3876066
TACATGG53100.023.0807322
GTACATG54150.022.9923231
ACATGGG53250.022.3020483
GTATCAA98550.022.0845641
GGTATCA75850.021.3011651
AGTACTT51200.021.10745212-13
GAGTACT50450.021.04460312-13
GTACTTT53050.020.01080914-15
AGAGTAC82250.019.80991610-11
CGATCGT1101.3456483E-619.4324224-25
ATTCGCG1101.3466743E-619.43087480-81
CATGGGG24550.019.349644
TCAACGC112850.019.1528854
TATCAAC114100.019.0679592
ATCAACG113550.019.0348133
CGTAGCC1250.006028981419.0013473
CAACGCA113750.019.0013455
AACGCAG115750.018.67086
Produced by FastQC (version 0.11.5)