##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937346_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1256179 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10806978941695 31.0 31.0 34.0 31.0 34.0 2 32.3454889788796 34.0 31.0 34.0 31.0 34.0 3 32.424703008090404 34.0 31.0 34.0 31.0 34.0 4 36.061758714323354 37.0 35.0 37.0 35.0 37.0 5 35.90446982476224 37.0 35.0 37.0 35.0 37.0 6 35.99424126657109 37.0 35.0 37.0 35.0 37.0 7 35.96024531535713 37.0 35.0 37.0 35.0 37.0 8 35.93538659697384 37.0 35.0 37.0 35.0 37.0 9 37.623496332927076 39.0 37.0 39.0 35.0 39.0 10-11 37.53934073089902 39.0 37.0 39.0 35.0 39.0 12-13 37.447442601731126 39.0 37.0 39.0 34.5 39.0 14-15 38.633037568690455 40.0 38.0 41.0 34.0 41.0 16-17 38.614727280108966 40.0 38.0 41.0 34.0 41.0 18-19 38.62113003003553 40.0 38.0 41.0 34.0 41.0 20-21 38.52389508183149 40.0 38.0 41.0 34.0 41.0 22-23 38.38740338757454 40.0 38.0 41.0 34.0 41.0 24-25 38.238597763535296 40.0 38.0 41.0 34.0 41.0 26-27 38.01133198373799 40.0 37.0 41.0 33.0 41.0 28-29 37.99789321426326 40.0 37.0 41.0 33.0 41.0 30-31 38.16042896752772 40.0 38.0 41.0 34.0 41.0 32-33 38.13016218230045 40.0 37.0 41.0 34.0 41.0 34-35 38.02558472956481 40.0 37.0 41.0 33.0 41.0 36-37 37.87721415498906 40.0 37.0 41.0 33.0 41.0 38-39 37.74042314033271 40.0 37.0 41.0 33.0 41.0 40-41 37.59283907787027 40.0 37.0 41.0 33.0 41.0 42-43 37.38690226472501 40.0 37.0 41.0 32.5 41.0 44-45 37.10840692289873 40.0 36.5 41.0 31.5 41.0 46-47 36.77839623174722 39.5 36.0 41.0 31.0 41.0 48-49 36.46603071695992 39.0 36.0 41.0 30.0 41.0 50-51 36.25046947927007 39.0 36.0 41.0 30.0 41.0 52-53 36.03324128169632 39.0 35.0 40.0 29.5 41.0 54-55 35.83004293177963 39.0 35.0 40.0 29.0 41.0 56-57 35.57888804063752 38.5 35.0 40.0 28.0 41.0 58-59 35.33671634376948 38.0 35.0 40.0 28.0 41.0 60-61 34.994253605576915 38.0 34.0 40.0 27.0 41.0 62-63 34.62958344312395 37.5 34.0 40.0 26.0 41.0 64-65 34.28725762809282 37.0 34.0 40.0 26.0 41.0 66-67 33.89347179024645 36.5 33.0 39.0 26.0 40.5 68-69 33.44122931524886 36.0 33.0 39.0 25.0 40.0 70-71 33.0451675278762 35.5 32.0 38.5 24.5 40.0 72-73 32.604913790152516 35.0 32.0 38.0 23.5 40.0 74-75 32.15425548429006 35.0 31.5 37.0 22.0 39.0 76-77 30.83993682429017 34.0 30.0 36.0 21.0 38.5 78-79 31.492249910243686 34.5 31.0 36.0 22.0 39.0 80-81 31.316144434829752 34.5 31.0 36.0 21.0 37.5 82-83 30.96902312488905 34.0 31.0 35.5 20.0 37.0 84-85 30.606910718934166 34.0 31.0 35.0 18.5 37.0 86-87 30.269258600884108 34.0 30.5 35.0 18.0 36.0 88-89 29.8908969183532 34.0 30.0 35.0 13.0 36.0 90-91 29.421625421217836 34.0 29.5 35.0 7.0 35.5 92-93 28.971450326744836 34.0 29.0 35.0 2.0 35.0 94-95 28.518606822753764 34.0 29.0 35.0 2.0 35.0 96-97 28.056613348893748 33.0 29.0 35.0 2.0 35.0 98-99 27.367051590577457 33.0 27.0 35.0 2.0 35.0 100-101 25.722785128552538 31.5 23.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.12481345345378614 1101 2 -0.0326722847951757 1101 3 -4.55887009820799E-4 1101 4 0.05019156459718488 1101 5 -0.14913776514178778 1101 6 -0.05519177898909078 1101 7 -0.011078383346053045 1101 8 -0.10522954941814788 1101 9 -0.1034200128564251 1101 10-11 0.004453560404790835 1101 12-13 -0.04603416134226279 1101 14-15 -0.014846480448376553 1101 16-17 -0.07339380651179539 1101 18-19 0.02451658107457888 1101 20-21 -0.14239369754980657 1101 22-23 -0.11196452663553202 1101 24-25 -0.026264349716811353 1101 26-27 0.0045662649334374805 1101 28-29 -0.044254333246151134 1101 30-31 -0.01310714473187602 1101 32-33 -0.09645904025953911 1101 34-35 -0.11879947740404617 1101 36-37 -0.007310325310832866 1101 38-39 -0.014490025806217943 1101 40-41 0.026703578715803644 1101 42-43 0.015947426409361753 1101 44-45 -0.021064078441249023 1101 46-47 -0.08837994186139753 1101 48-49 -0.1852941390175502 1101 50-51 -0.1346423506625669 1101 52-53 0.002949797660015463 1101 54-55 -0.14617554044880166 1101 56-57 -0.24070172075936824 1101 58-59 0.027758987547969127 1101 60-61 -0.06687310883372533 1101 62-63 -0.07300923303501605 1101 64-65 0.006297314209284366 1101 66-67 0.009986327873463097 1101 68-69 0.011790585712844859 1101 70-71 -0.1552512362952001 1101 72-73 -0.21475395202721614 1101 74-75 -0.08038755519653762 1101 76-77 0.07542068883356734 1101 78-79 -0.09294154892571527 1101 80-81 -0.07149154877187769 1101 82-83 -0.11804320155334835 1101 84-85 -0.2588842123311288 1101 86-87 -0.2291606162648243 1101 88-89 -0.27093725027649995 1101 90-91 -0.17878511234872718 1101 92-93 -0.08390474154308691 1101 94-95 -0.1967911546801311 1101 96-97 -0.43962585404681676 1101 98-99 -0.5996174592825518 1101 100-101 -0.09107662113862247 1102 1 0.07305779046608762 1102 2 0.09081793656738313 1102 3 -0.02987776536786413 1102 4 0.029893490499723896 1102 5 -0.022186094725071825 1102 6 -0.026037667312181156 1102 7 0.06650048830577049 1102 8 0.075269097493873 1102 9 0.12297397478376126 1102 10-11 0.06272570872253169 1102 12-13 0.09368522093031828 1102 14-15 0.05074111500244527 1102 16-17 0.052810595300513796 1102 18-19 0.011883520113251222 1102 20-21 0.0523192811297335 1102 22-23 0.09613865016065404 1102 24-25 0.08957697714275525 1102 26-27 0.13412531614901724 1102 28-29 -0.05104192255654283 1102 30-31 -0.06776214019234317 1102 32-33 -0.07248833011861677 1102 34-35 -0.0678000124988074 1102 36-37 -0.07237598406950241 1102 38-39 -0.09994575548687124 1102 40-41 -0.11352458218105 1102 42-43 -0.18456650579150136 1102 44-45 -0.05661651992073047 1102 46-47 -0.16905052086024597 1102 48-49 -0.06190687953606755 1102 50-51 -0.011150813493223666 1102 52-53 -0.02031278912841117 1102 54-55 -0.10252803631497187 1102 56-57 -0.03449462154314631 1102 58-59 -0.13535390428832983 1102 60-61 -0.2461639547659118 1102 62-63 -0.15738238268172466 1102 64-65 -0.05006614671344778 1102 66-67 -0.26785537618404476 1102 68-69 -0.10872722811804181 1102 70-71 -0.15729369738021148 1102 72-73 -0.085268147389435 1102 74-75 -0.26359665443907687 1102 76-77 -0.2407619802366412 1102 78-79 -0.27406969973472073 1102 80-81 -0.18580379849445805 1102 82-83 -0.15791181824567246 1102 84-85 -0.32001144207537635 1102 86-87 -0.09964235505963615 1102 88-89 -0.08004362009715393 1102 90-91 -0.13733654065699596 1102 92-93 -0.3708097227489766 1102 94-95 -0.23011122029502573 1102 96-97 0.11735932558273987 1102 98-99 0.1612329027836168 1102 100-101 0.3764423593742805 1103 1 0.02637220228103132 1103 2 -0.008821610754388587 1103 3 -0.009831967203389524 1103 4 0.03458386973908745 1103 5 -0.07656815658630478 1103 6 -0.050898340217109705 1103 7 -0.026494729628772973 1103 8 -0.07680238180032717 1103 9 -0.14505023217540014 1103 10-11 0.045128359532881746 1103 12-13 0.06729048929226877 1103 14-15 0.05940029396135316 1103 16-17 0.0013066491976232442 1103 18-19 -0.07608603466926667 1103 20-21 -0.012912878260458172 1103 22-23 -0.0921163004456389 1103 24-25 0.0045912694060632475 1103 26-27 0.06791719292993292 1103 28-29 -0.016965998278571703 1103 30-31 -0.054462245361833084 1103 32-33 0.15188672044363472 1103 34-35 0.06573197430557087 1103 36-37 0.08429890739019896 1103 38-39 0.1322522867427196 1103 40-41 0.10819999511851819 1103 42-43 0.1388315696968121 1103 44-45 0.19461618778900203 1103 46-47 0.1710428732376741 1103 48-49 0.31207592030932574 1103 50-51 0.3693349661542342 1103 52-53 0.40270033907948033 1103 54-55 0.4346560057887743 1103 56-57 0.36365996861047023 1103 58-59 0.3703624392293676 1103 60-61 0.26343377195522777 1103 62-63 0.3219659710113447 1103 64-65 0.3447078512550732 1103 66-67 0.19407203439942577 1103 68-69 0.26940617083386087 1103 70-71 0.3086024005622434 1103 72-73 0.2737968779141582 1103 74-75 0.12563886677909153 1103 76-77 0.05062738056660265 1103 78-79 0.3895253662883498 1103 80-81 0.280282525876995 1103 82-83 0.19471472221761132 1103 84-85 0.24971412849155072 1103 86-87 0.4726027887169373 1103 88-89 0.45706030892692695 1103 90-91 0.4415665281346328 1103 92-93 0.4548332043784562 1103 94-95 0.3045183044647466 1103 96-97 0.469991484074054 1103 98-99 0.4433010562052182 1103 100-101 0.7381306666849063 1104 1 -0.057043119285452804 1104 2 0.014152971660429614 1104 3 -0.042328897268042454 1104 4 0.03653679246548336 1104 5 -0.11684348910098663 1104 6 -0.007869312683858709 1104 7 -0.10139190528825281 1104 8 -0.11722936172368748 1104 9 -0.002841950007656635 1104 10-11 -0.01595595642635317 1104 12-13 -0.1670167779474454 1104 14-15 -0.12497891332956357 1104 16-17 -0.13796115272866416 1104 18-19 -0.0029152353024883837 1104 20-21 -0.17504892447963272 1104 22-23 -0.14460757147284653 1104 24-25 -0.2047847939751577 1104 26-27 -0.2656275837415123 1104 28-29 -0.2887033597616053 1104 30-31 -0.174876760607944 1104 32-33 -0.31463896344919107 1104 34-35 -0.26937735977737276 1104 36-37 -0.12959236511649408 1104 38-39 -0.15914560646790932 1104 40-41 -0.07054606574757116 1104 42-43 -0.22330706562446068 1104 44-45 -0.21298708039760328 1104 46-47 -0.28651896931533116 1104 48-49 -0.12561271427220078 1104 50-51 0.10473982774824009 1104 52-53 0.2011082262242283 1104 54-55 0.1020562513836012 1104 56-57 0.024261047156436177 1104 58-59 0.2019561425330636 1104 60-61 -0.020044242869232676 1104 62-63 -0.009416905407114484 1104 64-65 0.020753848503225925 1104 66-67 0.06707657642424181 1104 68-69 -0.017576378617988553 1104 70-71 0.0070580011599545855 1104 72-73 0.1066046851666087 1104 74-75 0.12834447847296104 1104 76-77 0.21932864996637136 1104 78-79 0.060053802026832415 1104 80-81 0.12026676934135949 1104 82-83 -0.004451956778112276 1104 84-85 -0.11193072807219195 1104 86-87 -0.051060679792183095 1104 88-89 0.011039388981963327 1104 90-91 0.08103591015295208 1104 92-93 0.09757595119386053 1104 94-95 -0.12853398800022475 1104 96-97 -0.1113793715132303 1104 98-99 -0.15304405630975992 1104 100-101 -0.051836747615812584 1105 1 -0.03772486079128612 1105 2 -0.09888353019717044 1105 3 -0.1184275107617907 1105 4 -0.07639159625517067 1105 5 -0.1926351430735238 1105 6 -0.09325466573075403 1105 7 -0.028341673317846983 1105 8 -0.04044194231014586 1105 9 -0.08214050788575378 1105 10-11 0.004814161818245566 1105 12-13 -0.12256745463856333 1105 14-15 -0.02065765936030317 1105 16-17 -0.04623073424137658 1105 18-19 -0.04712111236327843 1105 20-21 -0.10953875315481554 1105 22-23 -0.22924443912600623 1105 24-25 -0.008364310037251244 1105 26-27 0.043072865681985206 1105 28-29 -0.049131165744789485 1105 30-31 -0.15581012713345643 1105 32-33 -0.0804880170630824 1105 34-35 -0.08675689158359745 1105 36-37 -0.07574282179865577 1105 38-39 -0.11188994215741133 1105 40-41 0.11376267981288635 1105 42-43 -0.25488300245162776 1105 44-45 -0.12161691124015306 1105 46-47 0.0026390721551337037 1105 48-49 0.052781884875983565 1105 50-51 0.0901209124987119 1105 52-53 0.33497120317439766 1105 54-55 0.30488454727520065 1105 56-57 0.300457780350591 1105 58-59 0.3114651657628187 1105 60-61 0.22230911686790478 1105 62-63 0.3203023480346161 1105 64-65 0.32193749566284424 1105 66-67 0.04565905729103292 1105 68-69 0.06860742700533251 1105 70-71 0.14120736290907132 1105 72-73 0.3846232397322993 1105 74-75 0.34748277843349484 1105 76-77 0.2984412685841207 1105 78-79 0.14274833922081953 1105 80-81 0.06159852383099462 1105 82-83 0.17564587155356293 1105 84-85 0.16656245083229848 1105 86-87 0.304638058223361 1105 88-89 0.16034874283856126 1105 90-91 0.18500407121750229 1105 92-93 0.14263203712003403 1105 94-95 0.35666230762965156 1105 96-97 0.30323182502060675 1105 98-99 0.2584208690282779 1105 100-101 0.20452940372008754 1106 1 -0.3654337049291918 1106 2 -0.12616521877534836 1106 3 -0.2755560768431877 1106 4 -0.10881109995735017 1106 5 -0.3501846466659657 1106 6 -0.19187718506461948 1106 7 -0.24613492441850582 1106 8 -0.15009113463439405 1106 9 -0.3038803910595931 1106 10-11 -0.3081193489529994 1106 12-13 -0.4558821439860665 1106 14-15 -0.2485050989263513 1106 16-17 -0.18289612884971973 1106 18-19 -0.2841186848858399 1106 20-21 -0.42203471447123064 1106 22-23 -0.3845126199078521 1106 24-25 -0.31374944499938806 1106 26-27 -0.2873595752191207 1106 28-29 -0.4256445290629287 1106 30-31 -0.39117256621791796 1106 32-33 -0.417733183749462 1106 34-35 -0.3456444339059459 1106 36-37 -0.3993748815699476 1106 38-39 -0.29576571895628945 1106 40-41 -0.3855490675441331 1106 42-43 -0.7245393821086097 1106 44-45 -0.5456956036942486 1106 46-47 -0.4626809805641656 1106 48-49 -0.6164741621763596 1106 50-51 -0.3436253216193563 1106 52-53 -0.4364079112535464 1106 54-55 -0.5019441503605933 1106 56-57 -0.5462828648390143 1106 58-59 -0.6092449597808027 1106 60-61 -0.46923375779696386 1106 62-63 -0.5425788579742132 1106 64-65 -0.5103141816862689 1106 66-67 -0.17675901991762544 1106 68-69 -0.0973248596637859 1106 70-71 -0.2278512001364561 1106 72-73 -0.2624791800640125 1106 74-75 -0.4051780282303028 1106 76-77 -0.39768894101288765 1106 78-79 -0.25011529165630364 1106 80-81 -0.1825112466742418 1106 82-83 -0.28190146791409987 1106 84-85 -0.41670956563211803 1106 86-87 -0.24135934726534813 1106 88-89 -0.3986437747761933 1106 90-91 -0.48084454612012806 1106 92-93 -0.4591501044003117 1106 94-95 -0.7215970167001124 1106 96-97 -0.4234663423619516 1106 98-99 -0.40577219284861954 1106 100-101 -0.2727904161271901 1107 1 -0.007074326578447199 1107 2 0.007659476466820081 1107 3 0.011443066749599495 1107 4 0.0719831727675313 1107 5 0.03414296298943498 1107 6 -0.03332355861589065 1107 7 0.024437872269871264 1107 8 -6.660619642957499E-4 1107 9 -0.11398834817558878 1107 10-11 -0.09539606394649525 1107 12-13 -0.08607938743400467 1107 14-15 -0.06258985003560014 1107 16-17 0.0022026623570994275 1107 18-19 -0.05357531595419118 1107 20-21 -0.1251256665774534 1107 22-23 -0.07859037033778549 1107 24-25 -0.09054710461417415 1107 26-27 -0.10730843290472336 1107 28-29 -0.0883793665738466 1107 30-31 -0.011029626458352482 1107 32-33 0.012662458396846432 1107 34-35 -0.013147792433422012 1107 36-37 -0.03678431201073096 1107 38-39 0.01663527193037595 1107 40-41 -0.09806257428954979 1107 42-43 -0.18108934081013217 1107 44-45 -0.1107443317427581 1107 46-47 -0.10992179978199346 1107 48-49 -0.058757469706790744 1107 50-51 0.22790871952970093 1107 52-53 0.1266538898026468 1107 54-55 0.06275782961413512 1107 56-57 0.16129108156566474 1107 58-59 0.0091903468863066 1107 60-61 0.11729916960765507 1107 62-63 0.2228926779001128 1107 64-65 0.2846911241645529 1107 66-67 0.12376001266044057 1107 68-69 -0.21025647022125327 1107 70-71 -0.10826237244586423 1107 72-73 0.07281133429221853 1107 74-75 0.09241099177143752 1107 76-77 0.11899560782376284 1107 78-79 -0.0021902459651101935 1107 80-81 0.006980090330991118 1107 82-83 -0.10060203253410549 1107 84-85 -0.18780896974425687 1107 86-87 -0.13991141032126464 1107 88-89 -0.03579794329333552 1107 90-91 -0.07142984303332511 1107 92-93 -0.08995696727969715 1107 94-95 -0.019079193330291844 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76-77 0.2655941216321196 2316 78-79 0.3009287918703798 2316 80-81 0.1373843449730927 2316 82-83 0.1250010842900764 2316 84-85 0.05863846221011215 2316 86-87 0.07128566433557992 2316 88-89 0.11701747812403696 2316 90-91 -0.2717378175818439 2316 92-93 -0.26309441994555627 2316 94-95 -0.05512369883468082 2316 96-97 0.02606962051508077 2316 98-99 -0.1876747450527958 2316 100-101 -0.16809204025712532 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 10.0 5 28.0 6 44.0 7 116.0 8 282.0 9 576.0 10 932.0 11 1574.0 12 2413.0 13 3368.0 14 4313.0 15 6066.0 16 8396.0 17 11906.0 18 10498.0 19 6477.0 20 5406.0 21 5602.0 22 6015.0 23 6518.0 24 7520.0 25 9176.0 26 11412.0 27 14235.0 28 17737.0 29 21866.0 30 28404.0 31 35662.0 32 45924.0 33 60980.0 34 83770.0 35 120159.0 36 185947.0 37 284093.0 38 228927.0 39 19819.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.30861431692257 20.5962955323231 15.376798216656487 21.718291934097845 2 15.682876405353058 21.697544697053527 39.41763076758965 23.201948130003768 3 19.601824262306565 25.3649360481269 30.45314401848781 24.580095671078723 4 12.899764205391273 17.643677886414185 37.961021447597396 31.495536460597144 5 13.219836721565207 38.50402941368488 34.44253009918679 13.833603765563124 6 31.27460338056917 37.74732741114125 17.20089254795694 13.777176660332643 7 27.970456439727144 33.48981315560919 20.872582649447253 17.667147755216416 8 26.332552924384185 35.432291098641194 19.673629315567286 18.56152666140733 9 26.194435665617714 17.03045505457423 20.696413488841955 36.078695790966094 10-11 24.59669362407746 27.484100593944017 27.528958850609662 20.390246931368857 12-13 25.766063560416708 24.744234147413323 28.139597740749895 21.35010455142007 14-15 22.400032161021638 26.148104688901817 26.880245570097895 24.57161757997865 16-17 21.521932781872646 29.659586730871958 27.268167991982033 21.550312495273367 18-19 21.392293614206256 28.17886622845948 29.768249588633466 20.660590568700798 20-21 22.39139485694316 26.84704966410042 30.032622739275215 20.72893273968121 22-23 21.950942828461503 27.021287774635063 29.894074593585092 21.13369480331835 24-25 22.048546637864913 27.116158715336695 29.91402063488841 20.92127401190998 26-27 21.788116875285343 27.33995961500112 29.994956850622874 20.876966659090666 28-29 21.36268432268999 27.837293705464223 29.91805138386805 20.88197058797774 30-31 21.716637988234876 27.201482601287008 29.910589366650463 21.171290043827646 32-33 21.550360876260818 27.367249160847763 29.859968149430994 21.222421813460425 34-35 22.08562633191607 27.201975196210093 29.757303696368115 20.95509477550572 36-37 21.968714550122396 27.10947917288594 29.774076064242642 21.147730212749018 38-39 21.666700499491913 27.542626374558033 29.86666284554758 20.924010280402474 40-41 21.958782131679534 27.509784636498715 29.587156602345928 20.944276629475826 42-43 21.74674766325377 27.555214088947626 29.300886928552096 21.39715131924651 44-45 22.003751057771225 27.38837379067792 29.22951267295505 21.378362478595804 46-47 22.147002935091255 27.863743940951093 28.831121997740766 21.158131126216883 48-49 22.261844156702253 27.93253506567737 28.54781386562499 21.257806911995388 50-51 21.967251482471845 28.114623791673004 28.6155874282248 21.30253729763035 52-53 22.37356772714662 28.348962108429827 28.008166049914163 21.269304114509385 54-55 22.056291340644922 28.049465880260694 28.269339003438205 21.624903775656175 56-57 22.235684564062925 28.246491941037068 28.170905579539223 21.346917915360788 58-59 22.610591324962446 27.986656360279866 28.10801645306919 21.2947358616885 60-61 22.26955712521862 27.947927803282813 28.698696602952285 21.08381846854628 62-63 21.976485835219346 28.05949629790022 28.664943451530394 21.29907441535004 64-65 21.928005483294978 28.19928529293994 28.417606089577994 21.455103134187087 66-67 22.017562783647872 28.034022221355396 28.23705857206656 21.711356422930173 68-69 21.982933960844754 28.018697972183897 28.279528634056135 21.718839432915214 70-71 22.15966036687447 27.95509238731104 28.318336797542386 21.5669104482721 72-73 22.150426014126968 27.740115063219495 28.42755690072832 21.68190202192522 74-75 21.90002380234027 28.420591332923095 27.94561921509594 21.733765649640695 76-77 21.97700327739916 27.951470292052328 28.07155668101441 21.999969749534102 78-79 22.072013622262432 28.009423816191802 28.052570533339594 21.865992028206172 80-81 22.09390540679314 28.098463674364883 28.052888959296407 21.754741959545573 82-83 22.03350971562038 27.925811174097838 28.22628730278393 21.814391807497852 84-85 21.93947755036406 27.96959190115099 28.23543787765831 21.85549267082664 86-87 21.854090858070386 28.005921130666888 28.093687285012724 22.046300726250003 88-89 21.78666416171581 27.999393398552275 27.84985260858524 22.364089831146675 90-91 21.93319411792336 27.800064804428352 28.033766769340023 22.23297430830826 92-93 21.986767514415067 27.8045394224102 28.028875509393377 22.17981755378135 94-95 21.759398939163923 27.798068587358966 27.931608472996285 22.510924000480824 96-97 21.566432809336884 27.722402619371923 28.127122010477805 22.584042560813387 98-99 21.676037826421656 27.86558071072213 27.721928513826672 22.73645294902954 100-101 21.456189747891063 27.798263489287034 27.981978058549018 22.763568704272885 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 176.0 1 131.0 2 119.5 3 169.5 4 166.5 5 155.0 6 292.0 7 663.0 8 1141.5 9 1429.5 10 1434.5 11 1359.5 12 1446.5 13 1622.5 14 1760.0 15 1862.5 16 1927.0 17 2014.0 18 2165.5 19 2307.5 20 2512.0 21 2882.0 22 3338.0 23 3975.5 24 4843.0 25 6006.0 26 7513.5 27 9439.0 28 11188.0 29 13191.0 30 16045.0 31 18625.0 32 21344.0 33 24444.0 34 27631.0 35 30659.5 36 33738.5 37 36501.5 38 38666.0 39 40137.5 40 41079.0 41 42475.5 42 43741.5 43 45443.5 44 47565.0 45 48761.5 46 48810.0 47 51500.5 48 56862.0 49 55189.5 50 49019.5 51 44434.5 52 40400.5 53 38197.0 54 36259.5 55 32865.0 56 28341.0 57 24052.0 58 21368.5 59 19216.0 60 16137.5 61 12774.0 62 9800.5 63 7296.0 64 5411.0 65 4097.0 66 2889.5 67 2168.5 68 1602.0 69 1031.0 70 714.0 71 481.0 72 334.0 73 257.0 74 219.0 75 175.5 76 113.0 77 60.0 78 31.5 79 19.0 80 15.5 81 11.5 82 10.5 83 6.0 84 3.5 85 2.0 86 1.0 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04457963395344135 2 0.0 3 0.0 4 7.960648920257383E-5 5 0.013135070718424683 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.007841239186453523 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.55277870430886E-4 24-25 4.3783569061415606E-4 26-27 0.0015523265394501897 28-29 0.00206976871926692 30-31 6.766551582218776E-4 32-33 3.9803244601286916E-5 34-35 0.0 36-37 3.1842595681029533E-4 38-39 1.9901622300643457E-4 40-41 3.9803244601286916E-5 42-43 0.012657431783209239 44-45 0.0 46-47 0.0 48-49 3.9803244601286916E-5 50-51 0.0 52-53 1.9901622300643457E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.77638935215443E-4 84-85 3.9803244601286916E-5 86-87 0.0 88-89 0.0 90-91 0.0073237970066367935 92-93 0.0022289816976720674 94-95 0.0 96-97 0.0 98-99 0.0015921297840514766 100-101 0.012299202581797657 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1256179.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.84667909128058 #Duplication Level Percentage of deduplicated Percentage of total 1 80.71236991588341 49.9179204088437 2 11.42810569641559 14.13580771254901 3 3.4583999831002106 6.416716617722668 4 1.46875515949156 3.633505160509485 5 0.7340621329466781 2.269965258470707 6 0.44968551981096944 1.6686933621446833 7 0.3178037813762623 1.375857593654123 8 0.2231236192880735 1.1039563903835634 9 0.17565995497168266 0.9777586377890208 >10 0.94167113365496 10.749818225184793 >50 0.0622917714301626 2.650268655224279 >100 0.026020469231515343 2.811318077552201 >500 0.0010254311994655804 0.5236866395265876 >1k 8.972522995323828E-4 1.307551836565476 >5k 1.2817889993319755E-4 0.4571754238797805 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5767 0.4590906232312433 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4393 0.3497113070669069 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4098 0.32622739275214757 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3269 0.2602336132032139 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1321 0.10516017223660004 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 2.388194676077215E-4 0.0 0.0 0.0 0.0 7 4.77638935215443E-4 0.0 0.0 0.0 0.0 8 5.572454244180168E-4 0.0 0.0 0.0 0.0 9 5.572454244180168E-4 0.0 0.0 0.0 0.0 10-11 6.368519136205907E-4 7.960648920257383E-5 0.0 0.0 0.0 12-13 6.368519136205907E-4 1.5921297840514766E-4 0.0 7.960648920257383E-5 0.0 14-15 6.766551582218776E-4 1.5921297840514766E-4 0.0 7.960648920257383E-5 0.0 16-17 8.358681366270253E-4 1.5921297840514766E-4 0.0 1.1940973380386076E-4 0.0 18-19 0.0011144908488360337 1.5921297840514766E-4 0.0 1.5921297840514766E-4 0.0 20-21 0.0012737038272411813 2.388194676077215E-4 0.0 2.388194676077215E-4 0.0 22-23 0.0013135070718424684 3.1842595681029533E-4 0.0 3.5822920141158224E-4 0.0 24-25 0.0014329168056463292 3.1842595681029533E-4 0.0 6.766551582218776E-4 0.0 26-27 0.0014329168056463292 3.1842595681029533E-4 0.0 8.756713812283122E-4 0.0 28-29 0.0017513427624566244 3.1842595681029533E-4 0.0 0.0017115395178553376 0.0 30-31 0.001990162230064346 3.1842595681029533E-4 0.0 0.0037415049925209705 0.0 32-33 0.002029965474665633 3.1842595681029533E-4 0.0 0.006647141848414915 0.0 34-35 0.0021493752084694936 3.1842595681029533E-4 0.0 0.009234352747498564 0.0 36-37 0.002308588186874641 3.1842595681029533E-4 0.0 0.013493299919836266 0.0 38-39 0.0026668173882862236 3.1842595681029533E-4 0.0 0.02002103203444732 0.0 40-41 0.0027066206328875105 3.1842595681029533E-4 0.0 0.03323570924207458 0.0 42-43 0.0030648498342990926 3.1842595681029533E-4 0.0 0.048201729212158456 0.0 44-45 0.0035822920141158226 3.1842595681029533E-4 0.0 0.06551614061371827 0.0 46-47 0.0037813082371222573 3.1842595681029533E-4 0.0 0.09600542597830405 0.0 48-49 0.003980324460128692 3.1842595681029533E-4 0.0 0.12215615768134955 0.0 50-51 0.003980324460128692 3.1842595681029533E-4 0.0 0.16084491143380042 0.0 52-53 0.003980324460128692 3.5822920141158224E-4 0.0 0.20526533240883665 0.0 54-55 0.00413953743853384 3.980324460128692E-4 0.0 0.2597957775125997 0.0 56-57 0.004378356906141561 3.980324460128692E-4 0.0 0.35990093768483633 0.0 58-59 0.004378356906141561 3.980324460128692E-4 0.0 0.4450002746423878 0.0 60-61 0.004418160150742848 3.980324460128692E-4 0.0 0.5256018449599937 0.0 62-63 0.004497766639945422 3.980324460128692E-4 0.0 0.6268612992256677 0.0 64-65 0.004537569884546708 4.77638935215443E-4 0.0 0.7339718304477307 0.0 66-67 0.004855995841357004 4.77638935215443E-4 0.0 0.8871347156734828 0.0 68-69 0.004855995841357004 4.77638935215443E-4 0.0 1.0706276732854154 0.0 70-71 0.0052540282873698736 4.77638935215443E-4 0.0 1.224387607180187 0.0 72-73 0.005333634776572447 4.77638935215443E-4 0.0 1.4091542686193608 0.0 74-75 0.005333634776572447 4.77638935215443E-4 0.0 1.6533073710036548 0.0 76-77 0.005333634776572447 4.77638935215443E-4 0.0 1.9225365174867592 0.0 78-79 0.005333634776572447 4.77638935215443E-4 0.0 2.235987068721894 0.0 80-81 0.005413241265775021 4.77638935215443E-4 0.0 2.5771804814441257 0.0 82-83 0.005492847754977595 4.77638935215443E-4 0.0 2.95558992786856 0.0 84-85 0.005652060733382742 5.1744217981673E-4 0.0 3.340686319386011 0.0 86-87 0.006010289934794325 5.572454244180168E-4 0.0 3.698079652660966 0.0 88-89 0.006129699668598185 5.572454244180168E-4 0.0 4.193152409011773 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTCCG 90 8.944313E-4 26.390759 9 GTACCGT 90 8.950552E-4 26.387606 6 TACATGG 5310 0.0 23.080732 2 GTACATG 5415 0.0 22.992323 1 ACATGGG 5325 0.0 22.302048 3 GTATCAA 9855 0.0 22.084564 1 GGTATCA 7585 0.0 21.301165 1 AGTACTT 5120 0.0 21.107452 12-13 GAGTACT 5045 0.0 21.044603 12-13 GTACTTT 5305 0.0 20.010809 14-15 AGAGTAC 8225 0.0 19.809916 10-11 CGATCGT 110 1.3456483E-6 19.43242 24-25 ATTCGCG 110 1.3466743E-6 19.430874 80-81 CATGGGG 2455 0.0 19.34964 4 TCAACGC 11285 0.0 19.152885 4 TATCAAC 11410 0.0 19.067959 2 ATCAACG 11355 0.0 19.034813 3 CGTAGCC 125 0.0060289814 19.001347 3 CAACGCA 11375 0.0 19.001345 5 AACGCAG 11575 0.0 18.6708 6 >>END_MODULE