FastQCFastQC Report
Thu 26 May 2016
SRR937345_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937345_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1408468
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57850.41072995623613745No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40170.28520349770104825No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30020.21313938264838106No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29220.20745945239792454No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT23630.1677709397728596No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21730.15428110542802534No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18740.1330523661169441No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC15690.11139763203707859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG58650.027.3931272
GTACATG60600.026.7574541
GTATCAA88000.026.2072931
ACATGGG59300.025.969753
CGTACAC1401.7708662E-523.7655283
CATGGGG26300.022.9530754
TATCAAC103350.022.3061282
TCAACGC102350.022.1989774
ATCAACG102500.022.1657033
CAACGCA102700.022.0762545
GAGTACT48200.022.0264812-13
AGTACTT50750.021.94933712-13
AACGCAG104450.021.8320126
AGAGTAC79950.021.0329110-11
ATGGGGA14600.020.835535
TACGCTC1150.003708845720.6664124
GTACTTT52000.020.14136714-15
CATGGGA33700.020.0286334
GTCCTAC3603.6379788E-1219.8130721
GGTATCA64950.019.5476781
Produced by FastQC (version 0.11.5)