##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937345_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1408468 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.732465345325558 33.0 31.0 34.0 30.0 34.0 2 31.843596730632147 33.0 31.0 34.0 30.0 34.0 3 31.897658306755993 33.0 31.0 34.0 30.0 34.0 4 35.52176194276334 37.0 35.0 37.0 33.0 37.0 5 35.51758080410772 37.0 35.0 37.0 33.0 37.0 6 35.57040841538466 37.0 35.0 37.0 33.0 37.0 7 35.51487857729107 37.0 35.0 37.0 33.0 37.0 8 35.50427343752219 37.0 35.0 37.0 33.0 37.0 9 37.138948133716916 39.0 37.0 39.0 34.0 39.0 10-11 37.029586756674625 39.0 37.0 39.0 33.0 39.0 12-13 36.92793481996041 39.0 37.0 39.0 33.0 39.0 14-15 38.102556820602246 40.0 38.0 41.0 33.0 41.0 16-17 38.056792912583035 40.0 38.0 41.0 33.0 41.0 18-19 38.016781353924976 40.0 38.0 41.0 33.0 41.0 20-21 37.94742408063229 40.0 38.0 41.0 33.0 41.0 22-23 37.80970423183203 40.0 38.0 41.0 33.0 41.0 24-25 37.66607015565849 40.0 37.0 41.0 32.0 41.0 26-27 37.505813053615704 40.0 37.0 41.0 32.0 41.0 28-29 37.33301324559734 39.5 37.0 41.0 32.0 41.0 30-31 37.330757958292274 39.0 37.0 41.0 32.0 41.0 32-33 37.19292912583033 39.0 36.5 41.0 31.5 41.0 34-35 37.00034257079323 39.0 36.0 41.0 31.0 41.0 36-37 37.02742696319689 39.0 36.0 41.0 31.0 41.0 38-39 37.023927061175684 40.0 36.0 41.0 31.0 41.0 40-41 36.88672976595848 39.5 36.0 41.0 30.5 41.0 42-43 36.66397106643531 39.0 36.0 41.0 30.0 41.0 44-45 36.45176354734364 39.0 36.0 41.0 30.0 41.0 46-47 36.14035995137979 39.0 35.5 41.0 29.5 41.0 48-49 35.85087059130914 39.0 35.0 41.0 28.0 41.0 50-51 34.93449478440405 38.0 34.0 39.5 27.0 40.5 52-53 35.08439985856974 38.5 34.5 40.0 27.5 40.5 54-55 35.46489128613501 39.0 35.0 40.0 27.5 41.0 56-57 35.25054775827353 39.0 35.0 40.0 26.5 41.0 58-59 35.02439530042571 38.0 34.5 40.0 26.0 41.0 60-61 34.74962405961655 38.0 34.0 40.0 26.0 41.0 62-63 34.45109544554793 38.0 34.0 40.0 25.5 41.0 64-65 34.11663239775416 37.5 34.0 40.0 24.5 41.0 66-67 33.72578042241641 37.0 33.5 39.5 23.0 41.0 68-69 33.249073106382255 36.0 33.0 39.0 22.0 41.0 70-71 32.81291694237995 36.0 32.0 39.0 21.5 40.0 72-73 32.40685837377917 35.0 32.0 38.5 20.0 40.0 74-75 31.948620770936934 35.0 31.5 37.5 18.5 39.5 76-77 31.506036345873675 35.0 31.0 37.0 17.5 39.0 78-79 31.022854264349633 35.0 31.0 36.5 13.5 39.0 80-81 30.55687349659346 34.5 30.5 36.0 9.0 38.5 82-83 30.10073178801364 34.0 30.0 35.5 7.0 37.0 84-85 29.625385170270107 34.0 29.5 35.0 2.0 37.0 86-87 29.21058412402696 34.0 29.0 35.0 2.0 36.0 88-89 28.824557604432616 34.0 29.0 35.0 2.0 36.0 90-91 28.375208382441066 34.0 29.0 35.0 2.0 35.5 92-93 27.922271574505068 34.0 27.5 35.0 2.0 35.0 94-95 27.38954097643681 33.0 26.5 35.0 2.0 35.0 96-97 26.76588427994104 33.0 25.5 35.0 2.0 35.0 98-99 26.062629040915375 33.0 24.5 35.0 2.0 35.0 100-101 24.247550530079494 30.5 11.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.006535703724875219 1101 2 -0.05906273007923346 1101 3 -0.041686576595658664 1101 4 -0.08842400728713073 1101 5 0.008562215020532449 1101 6 -0.11898297601588581 1101 7 -0.0344407484964151 1101 8 0.027332292998316632 1101 9 -0.07177660843530731 1101 10-11 -0.012302464716022143 1101 12-13 -0.014708584803628355 1101 14-15 2.2137229955632165E-4 1101 16-17 -0.09648983908626008 1101 18-19 0.07660863466954737 1101 20-21 0.04897254826416031 1101 22-23 -0.12653009041476793 1101 24-25 0.1272844256961818 1101 26-27 0.1338359995944245 1101 28-29 0.1771461283786735 1101 30-31 0.02625928946616085 1101 32-33 -0.02511842541998277 1101 34-35 -0.10261674567514945 1101 36-37 -0.03665903853218566 1101 38-39 0.017137865911557526 1101 40-41 0.06803782719244822 1101 42-43 0.1289980254860339 1101 44-45 0.02901670301000081 1101 46-47 0.21723860211264423 1101 48-49 0.3529176735863686 1101 50-51 0.1912812738212466 1101 52-53 0.2639966634844271 1101 54-55 0.27844954696364965 1101 56-57 0.25654433387084197 1101 58-59 0.17112735955658565 1101 60-61 0.12396316987442191 1101 62-63 0.25327132930866014 1101 64-65 0.12982943268794145 1101 66-67 0.24457170681002793 1101 68-69 8.091993260137542E-4 1101 70-71 0.15013620371239966 1101 72-73 0.1713612932770303 1101 74-75 0.06860858903260691 1101 76-77 0.08307017460403543 1101 78-79 0.2541705999937385 1101 80-81 0.2810825880564529 1101 82-83 0.27202358096587176 1101 84-85 0.24265815625515685 1101 86-87 0.4335617263145437 1101 88-89 0.40122162110068516 1101 90-91 0.4057745384745317 1101 92-93 0.25076425841407257 1101 94-95 0.21362250673307415 1101 96-97 0.30785956033488304 1101 98-99 0.48851835513639585 1101 100-101 0.8615461519355172 1102 1 -0.05080603326510058 1102 2 -0.1306634492930172 1102 3 -0.14864097061035508 1102 4 -0.28901759293096774 1102 5 -0.16524471506780714 1102 6 -0.09531715272694186 1102 7 -0.0679319641484355 1102 8 0.01247487236200584 1102 9 0.04098395595842419 1102 10-11 -0.1105307648915641 1102 12-13 0.009730334735792212 1102 14-15 0.1272748046293728 1102 16-17 0.11335025800257625 1102 18-19 0.01870255415040134 1102 20-21 0.02530421492246404 1102 22-23 -0.017478128975874085 1102 24-25 0.005592884406652843 1102 26-27 0.006785495659499929 1102 28-29 -0.05053741086265262 1102 30-31 -0.005994051832402647 1102 32-33 0.08445625450645622 1102 34-35 0.07541732243430488 1102 36-37 0.2419386200906004 1102 38-39 0.3586812680705336 1102 40-41 0.24302040324285912 1102 42-43 0.117036578223086 1102 44-45 0.13071473278591839 1102 46-47 0.20661626799785182 1102 48-49 0.3082089275585318 1102 50-51 0.23217955058635908 1102 52-53 0.2631893677650723 1102 54-55 0.30824993920122523 1102 56-57 0.35380469495030553 1102 58-59 0.2816355699391764 1102 60-61 0.3089866890687958 1102 62-63 0.40511843530149605 1102 64-65 0.3142342238766531 1102 66-67 0.23085698769329355 1102 68-69 0.3645227470847914 1102 70-71 0.2929530811398209 1102 72-73 0.22074020163834973 1102 74-75 0.13275612491381494 1102 76-77 0.19107011017934994 1102 78-79 0.23089508990918617 1102 80-81 0.3401231078381777 1102 82-83 0.323483532237379 1102 84-85 0.30855425764120525 1102 86-87 0.4036022594832218 1102 88-89 0.506299646090774 1102 90-91 0.5980008632385427 1102 92-93 0.27164350479583277 1102 94-95 0.003301107027251504 1102 96-97 0.2630365023208725 1102 98-99 0.2656881074945936 1102 100-101 0.4394900882337254 1103 1 -0.13753149401912523 1103 2 -0.10045587695354286 1103 3 -0.056061538144927425 1103 4 -0.15468708677082077 1103 5 -0.22764332554270084 1103 6 -0.12322398768993992 1103 7 -0.056586290955166874 1103 8 -0.09140896725451597 1103 9 -0.059808870468927466 1103 10-11 -0.02663866377191937 1103 12-13 -0.0837563285563192 1103 14-15 -0.11663933545992933 1103 16-17 -0.17624839759539412 1103 18-19 -0.22819134670783114 1103 20-21 -0.25210685727506643 1103 22-23 -0.2561188213993262 1103 24-25 -0.2554911434162008 1103 26-27 -0.36041343382886026 1103 28-29 -0.5269470246477468 1103 30-31 -0.48581049881787663 1103 32-33 -0.32536168344807237 1103 34-35 -0.2166780323987112 1103 36-37 -0.2995591547573113 1103 38-39 -0.31920969294508694 1103 40-41 -0.21655587952449906 1103 42-43 -0.2411779666259477 1103 44-45 -0.3471319346286563 1103 46-47 -0.3132919015794542 1103 48-49 -0.43641337099320054 1103 50-51 -0.43940413435507963 1103 52-53 -0.3827646834301248 1103 54-55 -0.3545120405485491 1103 56-57 -0.38801711164384045 1103 58-59 -0.2976745489298409 1103 60-61 -0.18272650903495702 1103 62-63 -0.13656317043277966 1103 64-65 -0.2356649231604777 1103 66-67 -0.15564698431878554 1103 68-69 -0.3378916481696592 1103 70-71 -0.287276710578638 1103 72-73 -0.2168168113388873 1103 74-75 -0.2958525852901168 1103 76-77 -0.4974268457760971 1103 78-79 -0.3073865257372397 1103 80-81 -0.32481353012405023 1103 82-83 -0.1349876915413155 1103 84-85 -0.1682646986928944 1103 86-87 0.05802435137294992 1103 88-89 0.02067692181450198 1103 90-91 0.07135647885220564 1103 92-93 0.12792165616838247 1103 94-95 -0.08918977970948916 1103 96-97 0.17869607364756845 1103 98-99 0.33951855217041427 1103 100-101 0.638147718397498 1104 1 -0.110895961139029 1104 2 -0.19007063088028886 1104 3 -0.18063316258218975 1104 4 -0.1773400246981538 1104 5 -0.23446841103434224 1104 6 -0.0878049885254839 1104 7 -0.2320352367471088 1104 8 -0.0901803655271749 1104 9 -0.1446689443447582 1104 10-11 -0.14076069811363112 1104 12-13 -0.06085602984551031 1104 14-15 -0.13593811728362226 1104 16-17 -0.14428230458557323 1104 18-19 -0.20719838094916554 1104 20-21 -0.13984261897468286 1104 22-23 -0.05795132015982318 1104 24-25 -6.751088254048909E-4 1104 26-27 -0.06257885516564698 1104 28-29 -0.25349194491274574 1104 30-31 -0.2559748814983962 1104 32-33 -0.2985922174387028 1104 34-35 -0.08840301124030248 1104 36-37 -0.13755036071452054 1104 38-39 -0.03029143445997562 1104 40-41 -0.054737786399854826 1104 42-43 -0.16787574981142228 1104 44-45 0.02901898912141121 1104 46-47 -0.15851646782930118 1104 48-49 -0.17682430934748083 1104 50-51 -0.17982025303991378 1104 52-53 -0.1729847158115021 1104 54-55 -0.20732104240893534 1104 56-57 -0.1861696330941882 1104 58-59 -0.2769563565371058 1104 60-61 -0.1862921953024923 1104 62-63 -0.09536000844535408 1104 64-65 -0.1461695326833521 1104 66-67 -0.044420862674691364 1104 68-69 -0.11761071884426144 1104 70-71 -0.2790808880403617 1104 72-73 -0.2259116370736436 1104 74-75 -0.21279278420185932 1104 76-77 -0.2942822340429494 1104 78-79 -0.16147776733080477 1104 80-81 -0.11410671503227832 1104 82-83 -0.13290390358001147 1104 84-85 0.06710210864133614 1104 86-87 0.002423647797723305 1104 88-89 -0.2258139978215219 1104 90-91 -0.007868582550901948 1104 92-93 -0.1368798972400711 1104 94-95 -0.1799697842379615 1104 96-97 -0.1827223138061882 1104 98-99 -0.1157319178906242 1104 100-101 0.07099347470309425 1105 1 0.02044038142265947 1105 2 -0.008375390598789778 1105 3 0.09153412990090715 1105 4 0.04123895427238011 1105 5 0.07363315940182247 1105 6 0.041257645431635126 1105 7 0.08548051797657763 1105 8 0.1023922904721104 1105 9 0.22539508959846444 1105 10-11 0.19051794284575863 1105 12-13 0.2102661531021468 1105 14-15 0.225801347879532 1105 16-17 0.18195896306944093 1105 18-19 0.2568536769835532 1105 20-21 0.1397950399487229 1105 22-23 0.3059095963352618 1105 24-25 0.23605879309123878 1105 26-27 0.13674114043060825 1105 28-29 0.11327513347320206 1105 30-31 0.08916190409291858 1105 32-33 0.11898077040335409 1105 34-35 0.3606216442873844 1105 36-37 0.35003520592068327 1105 38-39 0.31632263707908237 1105 40-41 0.3497391239799512 1105 42-43 0.18756924354001825 1105 44-45 0.2982816813266993 1105 46-47 0.2245803982475465 1105 48-49 0.18501158542166252 1105 50-51 0.3333831853197111 1105 52-53 0.26234147001888886 1105 54-55 0.370271996544723 1105 56-57 0.4942185370622951 1105 58-59 0.46623598656177023 1105 60-61 0.6181739399472619 1105 62-63 0.6174296805359774 1105 64-65 0.4102492044870232 1105 66-67 0.6284450174074792 1105 68-69 0.6441242176410356 1105 70-71 0.506618562349928 1105 72-73 0.42945832077061397 1105 74-75 0.5724873730838027 1105 76-77 0.7077517669580438 1105 78-79 0.8482245345649169 1105 80-81 0.727982192283644 1105 82-83 0.5988913970578267 1105 84-85 0.42628989807655415 1105 86-87 0.2881336390761149 1105 88-89 0.45216421299155485 1105 90-91 0.6926729848557365 1105 92-93 0.5359785651801019 1105 94-95 0.5028392765532921 1105 96-97 0.4543849697913309 1105 98-99 0.5948480886213012 1105 100-101 0.8261239591857361 1106 1 -0.05951502855622337 1106 2 -0.08101757860476155 1106 3 -0.2083796130530935 1106 4 -0.16739993246724794 1106 5 -0.034431731114771935 1106 6 -0.16657885018243945 1106 7 -0.0669938556568539 1106 8 -0.11889858490795291 1106 9 -0.16337487346288526 1106 10-11 -0.27436025131569863 1106 12-13 -0.3664839908984021 1106 14-15 -0.43966029925751116 1106 16-17 -0.44443483330088895 1106 18-19 -0.401956720859161 1106 20-21 -0.36793943736510215 1106 22-23 -0.42436089544452926 1106 24-25 -0.5672479180508176 1106 26-27 -0.24396479181893227 1106 28-29 -0.07117739219615515 1106 30-31 -0.13544026752135352 1106 32-33 -0.005854094637321339 1106 34-35 0.12388617258169887 1106 36-37 -0.268232947462117 1106 38-39 -0.3545207619643591 1106 40-41 0.06022637599094338 1106 42-43 0.03800067205413171 1106 44-45 -0.04741134102275879 1106 46-47 0.04240609023824504 1106 48-49 0.14243040779805938 1106 50-51 -0.057088993200849814 1106 52-53 -0.021723615364123816 1106 54-55 0.25682392128043574 1106 56-57 0.16202861108288147 1106 58-59 0.02166258640071561 1106 60-61 0.04802776147827359 1106 62-63 0.09935725327416378 1106 64-65 0.062014321679114914 1106 66-67 0.0848866420202512 1106 68-69 0.016101280717577993 1106 70-71 0.007476834286009648 1106 72-73 0.13883655361878056 1106 74-75 0.08230090311759497 1106 76-77 0.10936285371085219 1106 78-79 0.33727619422498734 1106 80-81 0.33646833814115595 1106 82-83 0.20421526217587882 1106 84-85 0.1440946705843409 1106 86-87 0.23144327321441693 1106 88-89 0.37040772150406553 1106 90-91 0.15571141079360373 1106 92-93 0.2632515529694288 1106 94-95 0.3265367521734994 1106 96-97 0.36047368559299287 1106 98-99 0.6654312836854501 1106 100-101 0.678537007211709 1107 1 -0.01474992121928409 1107 2 -0.05996991043905098 1107 3 -0.008124019848189334 1107 4 0.04700765785241856 1107 5 0.05190637322571945 1107 6 0.047132837646728376 1107 7 -0.11062156333292705 1107 8 -0.024041696974649085 1107 9 -0.03429964505079397 1107 10-11 -0.027996581055347747 1107 12-13 0.0823815296574466 1107 14-15 0.16442958798253215 1107 16-17 0.058855772477443224 1107 18-19 0.16911557941698874 1107 20-21 0.0362104710522857 1107 22-23 0.06752469673417494 1107 24-25 0.15844177279227267 1107 26-27 0.2278014971333775 1107 28-29 0.10171692532610876 1107 30-31 0.1847595148977561 1107 32-33 0.09965432499088678 1107 34-35 0.06735737644021356 1107 36-37 0.026022111854580032 1107 38-39 0.10496411840740194 1107 40-41 0.21171138043066406 1107 42-43 0.015078455738340324 1107 44-45 0.13256161480079243 1107 46-47 0.07767125416469156 1107 48-49 0.16910535006191196 1107 50-51 -0.028637503279888676 1107 52-53 0.04913321581468466 1107 54-55 0.1588105595566489 1107 56-57 0.15789048252272408 1107 58-59 0.18349934661660683 1107 60-61 0.15822981077747755 1107 62-63 0.295008638133325 1107 64-65 0.2764841109648515 1107 66-67 0.18371634455080965 1107 68-69 0.13238826971677042 1107 70-71 0.13185975289907503 1107 72-73 0.24219836648931903 1107 74-75 0.1736703593666391 1107 76-77 0.1428668134917146 1107 78-79 0.37016476370486373 1107 80-81 0.27916395223963164 1107 82-83 0.034372323639345836 1107 84-85 0.19248183654536888 1107 86-87 0.22417044875111714 1107 88-89 0.31374000883887376 1107 90-91 0.16291329213909123 1107 92-93 0.10945215304921518 1107 94-95 0.2576023145032984 1107 96-97 0.2532981026962844 1107 98-99 0.5307700271739471 1107 100-101 0.996462155293095 1108 1 -0.06310737249714649 1108 2 -0.07858487460137908 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-0.46057624800346275 2316 54-55 -0.5215845972592561 2316 56-57 -0.4998430644822278 2316 58-59 -0.4460380413451617 2316 60-61 -0.7070614996837961 2316 62-63 -0.7260999884011881 2316 64-65 -0.7304206916027454 2316 66-67 -0.6823532273301893 2316 68-69 -0.5733559101355041 2316 70-71 -0.6754928928800368 2316 72-73 -0.9096165886953926 2316 74-75 -0.7641368636426407 2316 76-77 -0.5422023362963984 2316 78-79 -0.4951300863194241 2316 80-81 -0.6691057766209916 2316 82-83 -0.71877855111941 2316 84-85 -0.5056885633279116 2316 86-87 -0.6170142252949447 2316 88-89 -0.4007672441682111 2316 90-91 -0.3366044423989898 2316 92-93 -0.4104857630153589 2316 94-95 -0.7970675637268876 2316 96-97 -0.974489590774013 2316 98-99 -1.164535981322775 2316 100-101 -1.4950977566779713 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5383.0 3 985.0 4 776.0 5 941.0 6 1073.0 7 1272.0 8 1517.0 9 1986.0 10 2419.0 11 3118.0 12 4194.0 13 5513.0 14 6325.0 15 7835.0 16 10082.0 17 13536.0 18 11351.0 19 8025.0 20 7889.0 21 8204.0 22 9016.0 23 10089.0 24 11475.0 25 12884.0 26 14985.0 27 17847.0 28 21709.0 29 26163.0 30 32024.0 31 40015.0 32 51420.0 33 68231.0 34 93195.0 35 132881.0 36 197412.0 37 285223.0 38 246411.0 39 35056.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.471843509187906 19.536201609593725 15.129411563540193 22.86254331767817 2 14.48815140562964 20.457635266259548 41.054070895721686 24.000142432389126 3 18.789695059331606 24.750132109145657 30.421851920959558 26.03832091056318 4 11.853546812974573 16.576292479072926 38.72922070245456 32.84094000549794 5 12.984551321853157 37.91242046380213 34.855368016906965 14.247660197437753 6 30.868514284779103 36.97292612587228 18.0030696389652 14.155489950383421 7 27.639473311543348 33.02221055155978 21.338169541990545 18.000146594906326 8 25.813879845581827 34.74999555247194 20.359146786216222 19.076977815730018 9 25.779994734332863 16.670390584417895 21.0406808365295 36.50893384471974 10-11 24.160743311139136 26.902151480388163 28.15441810682251 20.78268710165019 12-13 25.241133129113997 24.271085338372753 28.557734424954372 21.930047107558877 14-15 22.068203689637812 25.60777986690835 27.22220542221289 25.101811021240945 16-17 21.302685727664183 29.06415474049684 27.62031193181535 22.012847600023626 18-19 21.028380009584165 27.76209075640536 30.033651299394105 21.17587793461637 20-21 22.047557566699385 26.615679687830756 30.00665650816872 21.330106237301134 22-23 21.520245138167716 26.677560273272277 29.98121902375212 21.82097556480789 24-25 21.487390157015614 26.700374022876794 30.114391094029365 21.697844726078223 26-27 21.304869440475446 26.92516238109605 30.062205764315436 21.707762414113066 28-29 21.04635656973054 27.344939672274865 30.084201223347783 21.524502534646814 30-31 21.335254768004557 26.86973384571591 30.02134927412468 21.773662112154852 32-33 21.05581151072379 26.890486295842425 30.157828741084863 21.89587345234893 34-35 21.683025441131893 26.872008777809558 29.810290871675765 21.634674909382777 36-37 21.461235949859578 26.71382006570311 29.984709121091097 21.840234863346218 38-39 21.235507225754308 27.118385403919383 29.89285876908891 21.7532486012374 40-41 21.489892351327192 27.10351753359641 29.79485886074756 21.611731254328838 42-43 21.267690258658504 27.039953669068943 29.552688094010808 22.13966797826174 44-45 21.371891223002947 26.98728506161072 29.480106068708416 22.160717646677917 46-47 21.581870535742873 27.318638831592875 29.13169909830811 21.96779153435615 48-49 21.62511798918324 27.37210292748375 28.96334249969491 22.039436583638096 50-51 21.44373204648619 27.510485225679204 29.064081301374223 21.98170142646038 52-53 21.696207333525262 27.773149599602288 28.513484130884482 22.017158935987965 54-55 21.469775551779662 27.40965581047781 28.815201629762743 22.305367007979786 56-57 21.65923238359478 27.613168285134993 28.618183758403585 22.109415572866645 58-59 21.947532774233018 27.353165034179096 28.624086838380176 22.075215353207714 60-61 21.655756883336398 27.292219403135316 29.16997315563719 21.882050557891095 62-63 21.40375601717195 27.493793860483777 29.152297721113396 21.950152401230877 64-65 21.328192886962466 27.676174642859557 28.907434617769113 22.088197852408864 66-67 21.349232323519786 27.56417462327646 28.62319820140302 22.46339485180073 68-69 21.276700184288227 27.494018013365075 28.834011475597016 22.39527032674968 70-71 21.50208235215442 27.38399797385072 28.791595464498993 22.322324209495868 72-73 21.480202584820653 27.186474517516274 28.963894478297124 22.369428419365946 74-75 21.254886755336745 27.78494363556811 28.613637738023666 22.346531871071477 76-77 21.350830888392963 27.414354156861116 28.58614674907423 22.648668205671694 78-79 21.39608863799606 27.387360593737537 28.67767674787678 22.538874020389628 80-81 21.524377511997294 27.503264551576358 28.502069713249472 22.470288223176876 82-83 21.441167670901685 27.331106548760214 28.791762438681307 22.435963341656798 84-85 21.43011613224493 27.40052286186843 28.606377492041474 22.562983513845168 86-87 21.296353274995315 27.414517822074135 28.773951852459184 22.51517705047136 88-89 21.364404907543346 27.315667419989538 28.43135127482079 22.888576397646332 90-91 21.340509467588227 27.189711045578886 28.680656096927613 22.78912338990527 92-93 21.47647473159209 27.12581198283897 28.790403483433057 22.60730980213588 94-95 21.231349469201145 27.059144531964257 28.65680604301744 23.052699955817157 96-97 21.006678893756643 27.07875943888186 28.834423479427613 23.080138187933883 98-99 21.181327066618003 27.074906742766565 28.522163667422966 23.221602523192466 100-101 21.00115846334479 26.758618138541117 29.011272698429973 23.228950699684116 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1979.0 1 1150.5 2 308.5 3 302.0 4 260.5 5 243.5 6 387.5 7 797.5 8 1324.5 9 1599.5 10 1547.0 11 1463.5 12 1534.5 13 1659.0 14 1789.0 15 1909.5 16 1995.0 17 2059.0 18 2216.0 19 2393.5 20 2629.0 21 3055.5 22 3596.5 23 4340.5 24 5303.0 25 6652.5 26 8440.0 27 10558.5 28 12580.0 29 14869.0 30 18045.5 31 21064.5 32 24045.0 33 27181.5 34 30771.5 35 34278.5 36 37699.0 37 40853.0 38 42867.5 39 44455.0 40 45898.0 41 47449.0 42 48827.0 43 50598.0 44 52519.0 45 53933.0 46 54855.0 47 57723.0 48 62988.0 49 61780.5 50 54755.5 51 49354.5 52 45366.0 53 42692.5 54 40607.0 55 36812.0 56 31834.0 57 27769.0 58 24862.0 59 22171.0 60 18669.5 61 14839.5 62 11204.5 63 8231.0 64 6282.5 65 4881.5 66 3539.5 67 2568.5 68 1874.0 69 1199.5 70 846.5 71 612.5 72 431.0 73 335.0 74 270.5 75 209.0 76 150.0 77 101.0 78 60.5 79 38.0 80 25.5 81 19.0 82 14.5 83 11.0 84 7.5 85 5.0 86 5.0 87 6.0 88 5.5 89 5.0 90 4.0 91 2.5 92 2.0 93 1.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3467597417903708 2 0.30479925706512323 3 0.30714222829343657 4 0.3057222457308224 5 0.28939244626075994 6 0.21945830505201397 7 0.2296821795028357 8 0.22670021612134603 9 0.22279526407415717 10-11 0.23401312631880883 12-13 0.23284164070465213 14-15 0.21622784472206683 16-17 0.22897218822152862 18-19 0.21473686303132197 20-21 0.21842881769411873 22-23 0.21700883513150457 24-25 0.20866643757614656 26-27 0.22236926930537293 28-29 0.22322125884294142 30-31 0.23202515073114904 32-33 0.23245114549993326 34-35 0.22052329197397455 36-37 0.2169023364393085 38-39 0.20873743670427727 40-41 0.2075659510901206 42-43 0.20784994760264347 44-45 0.2238247514320524 46-47 0.21292638526398894 48-49 0.2235407549195296 50-51 0.23262864332026004 52-53 0.24334951166799673 54-55 0.23823757444258586 56-57 0.20007554307233105 58-59 0.18729570000880388 60-61 0.1968450827423839 62-63 0.19904605571443584 64-65 0.1954960993079005 66-67 0.2078854471667088 68-69 0.19805206792060595 70-71 0.20206351865999084 72-73 0.21526935649230228 74-75 0.204690486400827 76-77 0.2140268717500149 78-79 0.2128908856999236 80-81 0.20153102519901056 82-83 0.21324588134057715 84-85 0.1947506084625281 86-87 0.19947205048322006 88-89 0.2071399563213364 90-91 0.204690486400827 92-93 0.21001542101062998 94-95 0.20909243234493083 96-97 0.20206351865999084 98-99 0.20923443060119223 100-101 0.19357912284837142 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1408468.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.67293094905107 #Duplication Level Percentage of deduplicated Percentage of total 1 80.51297823635811 49.65471347273366 2 11.62645727549448 14.340753934673273 3 3.457284169189015 6.396625435129246 4 1.441669348229067 3.5564789665877887 5 0.7651699301855703 2.3595136134312455 6 0.4587198885985266 1.6974360008696 7 0.3118724567408862 1.3463861942644115 8 0.22007125517213533 1.085795145928168 9 0.1566338167540323 0.8694059908461976 >10 0.9549211804808697 10.870078247657712 >50 0.06708218617742498 2.8529069700194722 >100 0.024955277984519953 2.67972571590584 >500 0.0011499887554447244 0.5239095092873973 >1k 9.199910043557796E-4 1.3559802231035265 >5k 1.1499887554447245E-4 0.41029057956241965 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5785 0.41072995623613745 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4017 0.28520349770104825 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3002 0.21313938264838106 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2922 0.20745945239792454 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2363 0.1677709397728596 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2173 0.15428110542802534 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1874 0.1330523661169441 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1569 0.11139763203707859 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 7.099912813070655E-5 0.0 0.0 0.0 0.0 3 7.099912813070655E-5 0.0 0.0 0.0 0.0 4 7.099912813070655E-5 0.0 0.0 0.0 0.0 5 7.099912813070655E-5 0.0 0.0 0.0 0.0 6 1.419982562614131E-4 0.0 0.0 0.0 0.0 7 2.1299738439211968E-4 0.0 0.0 0.0 0.0 8 2.839965125228262E-4 0.0 0.0 0.0 0.0 9 2.839965125228262E-4 0.0 0.0 0.0 0.0 10-11 2.839965125228262E-4 0.0 0.0 3.549956406535328E-5 0.0 12-13 2.839965125228262E-4 0.0 0.0 1.774978203267664E-4 0.0 14-15 4.2599476878423935E-4 0.0 0.0 2.4849694845747295E-4 0.0 16-17 4.614943328495926E-4 0.0 0.0 3.549956406535328E-4 0.0 18-19 7.099912813070656E-4 0.0 0.0 4.969938969149459E-4 0.0 20-21 8.519895375684787E-4 0.0 0.0 4.969938969149459E-4 0.0 22-23 9.229886656991852E-4 0.0 0.0 4.969938969149459E-4 0.0 24-25 9.939877938298918E-4 0.0 0.0 5.679930250456524E-4 0.0 26-27 9.939877938298918E-4 0.0 0.0 8.164899735031254E-4 0.0 28-29 0.001277984306352718 0.0 0.0 0.001810477767333017 0.0 30-31 0.001419982562614131 0.0 0.0 0.003833952919058154 0.0 32-33 0.001419982562614131 0.0 0.0 0.00681591630054783 0.0 34-35 0.001774978203267664 0.0 0.0 0.009655881425776091 0.0 36-37 0.00205897471579049 0.0 0.0 0.01363183260109566 0.0 38-39 0.0022719721001826097 0.0 0.0 0.022400224925237917 0.0 40-41 0.0023429712283133163 0.0 0.0 0.03876552395936578 0.0 42-43 0.0025914681767707894 0.0 0.0 0.054101335635598394 0.0 44-45 0.0033369590221432083 0.0 0.0 0.07174461897607898 0.0 46-47 0.003798453354992801 0.0 0.0 0.10525620745377247 0.0 48-49 0.0039049520471888606 0.0 0.0 0.1322713757075063 0.0 50-51 0.0039049520471888606 0.0 0.0 0.17128539661532957 0.0 52-53 0.003940451611254214 0.0 0.0 0.21732833120809275 0.0 54-55 0.003975951175319567 0.0 0.0 0.27395013589233125 0.0 56-57 0.00411794943158098 0.0 0.0 0.37636637822087543 0.0 58-59 0.00411794943158098 3.549956406535328E-5 0.0 0.46504428925612795 0.0 60-61 0.00411794943158098 7.099912813070655E-5 0.0 0.5509887338583482 0.0 62-63 0.00411794943158098 7.099912813070655E-5 0.0 0.6481865402692855 0.0 64-65 0.00411794943158098 7.099912813070655E-5 0.0 0.7555017224388485 0.0 66-67 0.00411794943158098 7.099912813070655E-5 0.0 0.9063748697165999 0.0 68-69 0.00411794943158098 7.099912813070655E-5 0.0 1.091966590650267 0.0 70-71 0.004153448995646333 7.099912813070655E-5 0.0 1.2461766969501613 0.0 72-73 0.004188948559711686 7.099912813070655E-5 0.0 1.4267274797865483 0.0 74-75 0.004188948559711686 7.099912813070655E-5 0.0 1.6666335337402058 0.0 76-77 0.004188948559711686 7.099912813070655E-5 0.0 1.9258158509813499 0.0 78-79 0.004188948559711686 7.099912813070655E-5 0.0 2.221420720953547 0.0 80-81 0.0048634402769534 7.099912813070655E-5 0.0 2.5517441645816588 0.0 82-83 0.005111937225410872 7.099912813070655E-5 0.0 2.904148337058421 0.0 84-85 0.005253935481672285 7.099912813070655E-5 0.0 3.2792367309729435 0.0 86-87 0.005395933737933698 7.099912813070655E-5 0.0 3.6344808685749337 0.0 88-89 0.005395933737933698 7.099912813070655E-5 0.0 4.105382585901845 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 5865 0.0 27.393127 2 GTACATG 6060 0.0 26.757454 1 GTATCAA 8800 0.0 26.207293 1 ACATGGG 5930 0.0 25.96975 3 CGTACAC 140 1.7708662E-5 23.765528 3 CATGGGG 2630 0.0 22.953075 4 TATCAAC 10335 0.0 22.306128 2 TCAACGC 10235 0.0 22.198977 4 ATCAACG 10250 0.0 22.165703 3 CAACGCA 10270 0.0 22.076254 5 GAGTACT 4820 0.0 22.02648 12-13 AGTACTT 5075 0.0 21.949337 12-13 AACGCAG 10445 0.0 21.832012 6 AGAGTAC 7995 0.0 21.03291 10-11 ATGGGGA 1460 0.0 20.83553 5 TACGCTC 115 0.0037088457 20.666412 4 GTACTTT 5200 0.0 20.141367 14-15 CATGGGA 3370 0.0 20.028633 4 GTCCTAC 360 3.6379788E-12 19.813072 1 GGTATCA 6495 0.0 19.547678 1 >>END_MODULE