FastQCFastQC Report
Thu 26 May 2016
SRR937345_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937345_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1408468
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62620.4445965403544845No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48970.34768273045607No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT46720.33170792662666104No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35310.25069792142952485No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15820.11232062070277776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT1102.7352053E-630.2256036
GAGTACT55350.021.84012812-13
GTACATG59450.021.4262221
CGGTTAC1451.05501385E-1021.29192528-29
TACATGG59800.021.0519032
GTACTTT57500.020.89884414-15
GTATCAA114000.020.6794721
AGTACTT56450.020.65725112-13
AGAGTAC89400.020.0569310-11
ACATGGG58850.020.0194383
GGTATCA90150.019.6655331
TCGTCCA1300.007564817618.268879
TCTATAC3951.8189894E-1118.0401783
TCAACGC131150.017.7852464
CAACGCA130900.017.782925
ATCAACG131150.017.7490233
GGACCGA2956.345181E-817.7108866
ACTTTTT68950.017.70387816-17
TACTTTT66600.017.6510514-15
TATCAAC133050.017.6383822
Produced by FastQC (version 0.11.5)