FastQCFastQC Report
Thu 26 May 2016
SRR937344_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937344_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1697618
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66400.39113628625521174No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47160.27780101294873166No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35780.2107659084670403No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33970.20010391030255334No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27070.1594587239296473No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT26500.15610107809884202No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT20390.12010947103529769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA103800.029.0252671
GTATAGG4350.027.310841
GTACATG71100.026.9351711
TACATGG71150.026.1761422
ATCAACG119150.024.8819853
TCAACGC119800.024.788844
CAACGCA120650.024.6134685
TATCAAC121550.024.4688662
AACGCAG121450.024.3325356
ACATGGG73050.024.3246083
GGTATCA73300.023.3390311
GAGTACT59450.022.3340112-13
CATGGGG34600.021.9723684
CGCAGAG135900.021.4637178
ACGCAGA136900.021.4116697
AGTACTT62750.021.04591612-13
GTACTTT63900.020.92580814-15
AGAGTAC96700.020.60860810-11
GCAGAGT142350.019.923839
GCACCGT3153.2014214E-1019.6077296
Produced by FastQC (version 0.11.5)