##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937344_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1697618 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.77175135984656 33.0 31.0 34.0 30.0 34.0 2 31.8795995329927 33.0 31.0 34.0 30.0 34.0 3 31.92727987097215 33.0 31.0 34.0 30.0 34.0 4 35.544856970178216 37.0 35.0 37.0 33.0 37.0 5 35.55869459442584 37.0 35.0 37.0 33.0 37.0 6 35.575904001960396 37.0 35.0 37.0 33.0 37.0 7 35.46581975450307 37.0 35.0 37.0 33.0 37.0 8 35.475652944301956 37.0 35.0 37.0 33.0 37.0 9 37.10747058525534 39.0 37.0 39.0 33.0 39.0 10-11 36.99323611083294 39.0 37.0 39.0 33.0 39.0 12-13 36.85171840779256 39.0 37.0 39.0 33.0 39.0 14-15 38.03268403138986 40.0 38.0 41.0 33.0 41.0 16-17 37.97262075449247 40.0 38.0 41.0 33.0 41.0 18-19 37.93697168620973 40.0 38.0 41.0 33.0 41.0 20-21 37.81936631209142 40.0 38.0 41.0 33.0 41.0 22-23 37.67173033038057 40.0 37.0 41.0 32.5 41.0 24-25 37.61142318236493 40.0 37.0 41.0 32.0 41.0 26-27 37.63375299979147 40.0 37.0 41.0 32.0 41.0 28-29 37.553139457757865 40.0 37.0 41.0 32.0 41.0 30-31 37.358218397778536 40.0 37.0 41.0 32.0 41.0 32-33 37.14138987687454 39.0 36.0 41.0 31.0 41.0 34-35 37.18811269673154 39.5 37.0 41.0 31.0 41.0 36-37 37.07362492622015 40.0 36.5 41.0 31.0 41.0 38-39 36.967406978483965 40.0 36.0 41.0 31.0 41.0 40-41 36.77241316951164 39.5 36.0 41.0 30.5 41.0 42-43 36.51216852083331 39.0 36.0 41.0 30.0 41.0 44-45 36.291564710082014 39.0 35.5 41.0 30.0 41.0 46-47 35.97665611462649 39.0 35.0 41.0 29.0 41.0 48-49 35.67208759567818 39.0 35.0 40.0 27.5 41.0 50-51 34.71806613737601 38.0 34.0 39.5 26.0 40.5 52-53 34.85778219835086 38.0 34.5 39.5 26.5 40.5 54-55 35.24898711017437 39.0 35.0 40.0 27.0 41.0 56-57 35.03323480311825 38.5 34.5 40.0 26.0 41.0 58-59 34.77460594786342 38.0 34.0 40.0 26.0 41.0 60-61 34.46686091924096 38.0 34.0 40.0 25.0 41.0 62-63 34.167037578536515 38.0 34.0 40.0 24.0 41.0 64-65 33.75776882667361 37.0 33.0 40.0 23.0 41.0 66-67 33.40452386814937 36.5 33.0 39.5 22.0 41.0 68-69 32.92674146951788 36.0 32.0 39.0 20.5 40.5 70-71 32.446867021909526 35.5 32.0 39.0 19.5 40.0 72-73 31.980409314698598 35.0 31.0 38.0 18.0 40.0 74-75 31.49007904016098 35.0 31.0 37.0 15.0 39.5 76-77 30.997522705343606 35.0 30.5 37.0 10.5 39.0 78-79 30.473636884151794 34.0 30.0 36.0 7.5 39.0 80-81 29.958286257567956 34.0 29.5 36.0 4.5 37.5 82-83 29.461405922887245 34.0 29.0 35.0 2.0 37.0 84-85 28.91168949669478 34.0 29.0 35.0 2.0 36.5 86-87 28.440656555243876 34.0 28.5 35.0 2.0 36.0 88-89 27.967852308352057 33.5 27.0 35.0 2.0 36.0 90-91 27.442455841066717 33.0 26.0 35.0 2.0 35.0 92-93 26.903951006645784 33.0 25.5 35.0 2.0 35.0 94-95 26.32442516514316 32.5 25.0 35.0 2.0 35.0 96-97 25.571773213997496 32.0 22.0 34.0 2.0 35.0 98-99 24.616043185215993 32.0 10.0 34.0 2.0 35.0 100-101 22.062933769552394 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.030833016277082237 1101 2 0.03661318208272846 1101 3 0.04660090924523175 1101 4 0.0309782146536719 1101 5 -0.01675470434778248 1101 6 -0.029896457660171905 1101 7 -0.013497542158482645 1101 8 -0.09196751131928949 1101 9 -0.08626384804880871 1101 10-11 -0.12583040558526193 1101 12-13 -0.09934056030263605 1101 14-15 -0.1821098274472419 1101 16-17 -0.19553425674934743 1101 18-19 0.0012487457551699777 1101 20-21 0.07031071285966561 1101 22-23 -0.02389068166439756 1101 24-25 0.04483904484020229 1101 26-27 -0.10416768212869698 1101 28-29 -0.0071772345143656935 1101 30-31 0.020522636875526246 1101 32-33 -0.18881512081441088 1101 34-35 -0.05798445549574893 1101 36-37 -0.058999016337402566 1101 38-39 -0.038595884060150354 1101 40-41 -0.055444929123375175 1101 42-43 -0.08435326086602402 1101 44-45 0.009634002125189056 1101 46-47 0.06731054346606413 1101 48-49 -0.09688035958476604 1101 50-51 -0.028631957016301612 1101 52-53 0.004682643346946236 1101 54-55 0.0374997275907063 1101 56-57 -0.08701866094502009 1101 58-59 -0.2055249816225384 1101 60-61 -0.06798742577530703 1101 62-63 -0.21589250739906873 1101 64-65 -0.3127314616489656 1101 66-67 -0.410934107981646 1101 68-69 -0.2912362556314818 1101 70-71 -0.28027814607502677 1101 72-73 -0.2206810280544289 1101 74-75 -0.36153956794990805 1101 76-77 -0.3393309444956678 1101 78-79 -0.2846533078389193 1101 80-81 -0.1202747405531639 1101 82-83 -0.2980188710200018 1101 84-85 -0.25459037074810453 1101 86-87 -0.17622702058677575 1101 88-89 -0.2347657455989065 1101 90-91 -0.24250425672603626 1101 92-93 -0.12419373753029816 1101 94-95 -0.04673097328635478 1101 96-97 0.24970750076450443 1101 98-99 0.37479079368312185 1101 100-101 0.5369002968674828 1102 1 -0.13014369708482576 1102 2 -0.14286338475071858 1102 3 -0.1242151385292587 1102 4 -0.2469867468809568 1102 5 -0.11328355144453184 1102 6 -0.18612120691016543 1102 7 -0.10913931329137938 1102 8 -0.12316838028858967 1102 9 -0.09459103132758173 1102 10-11 -0.07944986561894751 1102 12-13 -0.02932781223240255 1102 14-15 -0.0048744778935727595 1102 16-17 -0.09621882793291547 1102 18-19 -0.10640214797434311 1102 20-21 -0.1225728787947773 1102 22-23 -0.08973964987628591 1102 24-25 -0.11935414234863373 1102 26-27 -0.17655245965671185 1102 28-29 -0.05975874226493971 1102 30-31 -0.13984584691345958 1102 32-33 -0.23440495292497587 1102 34-35 -0.011010367035474644 1102 36-37 -0.062032729553067156 1102 38-39 0.16449416309058762 1102 40-41 0.04688162663338602 1102 42-43 0.03566964161716868 1102 44-45 0.10927702604349321 1102 46-47 0.13052858703913017 1102 48-49 -0.019679058841163055 1102 50-51 -0.16994148290967814 1102 52-53 -0.13464917181224934 1102 54-55 -0.0012291469483685091 1102 56-57 -0.08917367394059283 1102 58-59 -0.21045067183558785 1102 60-61 -0.09164278481144805 1102 62-63 -0.16425143568783795 1102 64-65 -0.11650764220063792 1102 66-67 -0.26689446605692524 1102 68-69 -0.23402926691088055 1102 70-71 -0.29332906062154507 1102 72-73 -0.11286657204768957 1102 74-75 -0.30190869544773946 1102 76-77 -0.14079494921920954 1102 78-79 -0.19641710584157934 1102 80-81 -0.21558025851198082 1102 82-83 -0.20621319053411113 1102 84-85 -0.2726327580345931 1102 86-87 -0.16989693459517952 1102 88-89 -0.1844848382103521 1102 90-91 -0.11266931157963 1102 92-93 0.08500655586541583 1102 94-95 -0.009359816223469153 1102 96-97 0.024757515247955553 1102 98-99 -0.05315832922592989 1102 100-101 0.35229561032828727 1103 1 -0.05177330962695237 1103 2 -0.14349285820559743 1103 3 -0.12950271555023818 1103 4 -0.04711139770223127 1103 5 -0.14415733975524603 1103 6 -0.16731898558503389 1103 7 -0.161038457320565 1103 8 -0.04299457022899844 1103 9 0.025434667774305808 1103 10-11 -0.06926530550969545 1103 12-13 -0.1409931992209934 1103 14-15 -0.20969091099544102 1103 16-17 -0.2275784415945239 1103 18-19 -0.2844969617538524 1103 20-21 -0.22182585965312995 1103 22-23 -0.20278328218689978 1103 24-25 -0.21814764527795205 1103 26-27 -0.35379230148171104 1103 28-29 -0.2659402281313774 1103 30-31 -0.39277572545523753 1103 32-33 -0.1913939175391448 1103 34-35 -0.12204976717365668 1103 36-37 -0.1783579162937201 1103 38-39 -0.3003859297548743 1103 40-41 -0.2185618638006943 1103 42-43 -0.09399359762307569 1103 44-45 -0.24039114344393653 1103 46-47 0.08546469548345925 1103 48-49 0.023291833477031787 1103 50-51 -0.2008053768023217 1103 52-53 -0.0024739754033404893 1103 54-55 0.11895514554043274 1103 56-57 0.026278353962794654 1103 58-59 0.09951477874113124 1103 60-61 0.0382719611130895 1103 62-63 0.0355967745155894 1103 64-65 -0.03813188348723884 1103 66-67 0.06485896429069982 1103 68-69 0.053100620601838955 1103 70-71 0.1014121409791997 1103 72-73 0.014221856910950237 1103 74-75 0.1452095976916432 1103 76-77 0.20056548991956547 1103 78-79 0.0482321813268527 1103 80-81 -0.023668785095548373 1103 82-83 -0.04968189458396566 1103 84-85 0.07849856030520641 1103 86-87 0.34313260052548245 1103 88-89 0.2636159235279152 1103 90-91 0.22052634799359083 1103 92-93 0.12971208102155174 1103 94-95 -0.20978727393095298 1103 96-97 -0.23129108387206543 1103 98-99 0.3022793225591549 1103 100-101 0.6854528230198262 1104 1 -0.025471804756023175 1104 2 -0.055924532516655745 1104 3 -0.05601573628485568 1104 4 -0.07287501545743424 1104 5 0.0055329676629654045 1104 6 -0.022832423286409664 1104 7 -0.09503716851422439 1104 8 -0.032986609954562596 1104 9 0.06537250182153542 1104 10-11 0.05970857720893008 1104 12-13 -0.08975310218146149 1104 14-15 -0.040035477160479616 1104 16-17 -0.0729214915168157 1104 18-19 0.0016838122880713513 1104 20-21 -0.0746191578601696 1104 22-23 -0.19959519227369782 1104 24-25 -0.1848168390158662 1104 26-27 -0.07149860526094898 1104 28-29 -0.11430666609809492 1104 30-31 -0.31943108347805094 1104 32-33 -0.16341696442894715 1104 34-35 -0.08930366202222473 1104 36-37 -0.07321235698967143 1104 38-39 -0.04161920370202665 1104 40-41 0.13115952659391183 1104 42-43 -0.03930329020486312 1104 44-45 0.01487647340152165 1104 46-47 0.050725489236540966 1104 48-49 0.11695659220490029 1104 50-51 -0.020820728760170937 1104 52-53 0.10008146222222791 1104 54-55 0.12864810638345858 1104 56-57 0.12563205549040646 1104 58-59 0.09023101391038324 1104 60-61 0.08755212486730812 1104 62-63 0.2674047044651715 1104 64-65 0.25816791561059205 1104 66-67 0.26734010602149283 1104 68-69 0.11728165998624718 1104 70-71 0.20033162848914543 1104 72-73 0.1065506162453218 1104 74-75 0.10803322075369337 1104 76-77 0.13370278570062055 1104 78-79 0.21441655644530755 1104 80-81 0.21250175430780516 1104 82-83 0.27255285769987836 1104 84-85 0.33884179975784434 1104 86-87 0.3359856007908242 1104 88-89 0.18989184180242802 1104 90-91 0.408933914044912 1104 92-93 0.5388671547488535 1104 94-95 0.6058999630956912 1104 96-97 0.8337991325715883 1104 98-99 0.9040696261867645 1104 100-101 1.4304744294790233 1105 1 0.0556606167309468 1105 2 0.1494678415950581 1105 3 -0.0034307282616268253 1105 4 0.04725106385791378 1105 5 -0.05007259510924911 1105 6 0.07742737651491893 1105 7 0.018483118793788833 1105 8 -0.09266720902233772 1105 9 0.029138070706686392 1105 10-11 -0.08438823642291737 1105 12-13 0.051205100615206334 1105 14-15 0.008840506640233059 1105 16-17 -0.06946462592500069 1105 18-19 -0.10376205001803385 1105 20-21 -0.009930535563242415 1105 22-23 0.10828973169665801 1105 24-25 -0.04151432507072883 1105 26-27 -0.038470027282805574 1105 28-29 -0.24079593025336266 1105 30-31 -0.12940930614176693 1105 32-33 -0.0872818685322585 1105 34-35 0.10688339383473533 1105 36-37 -0.05577635179368201 1105 38-39 -0.019626004343884063 1105 40-41 0.019101759343968183 1105 42-43 0.05633016284014758 1105 44-45 0.059983831848839486 1105 46-47 -0.0671273418171836 1105 48-49 -0.054162839292004605 1105 50-51 -0.20822807956631095 1105 52-53 -0.09084502971634123 1105 54-55 -0.03543036843888103 1105 56-57 -0.09478515242584251 1105 58-59 -0.09751598693014074 1105 60-61 -0.07136473919626951 1105 62-63 -0.07137805705389155 1105 64-65 -0.004286810453436374 1105 66-67 -0.07710934763480282 1105 68-69 -0.07732520178497992 1105 70-71 -0.16310651623462036 1105 72-73 0.030448377382327863 1105 74-75 0.11621882478823053 1105 76-77 0.01882237002093845 1105 78-79 -0.0067643864751474325 1105 80-81 0.07032211038636049 1105 82-83 0.11533577204235357 1105 84-85 -0.03458068056614039 1105 86-87 0.17257649251543583 1105 88-89 0.26677768271325775 1105 90-91 0.5406000713002044 1105 92-93 0.6166760490714651 1105 94-95 0.807461436313389 1105 96-97 0.9074951668040327 1105 98-99 0.9543649574315118 1105 100-101 1.1928565644220974 1106 1 -0.3337330810402399 1106 2 -0.20457137841082584 1106 3 -0.3247886650683611 1106 4 -0.014527514745680037 1106 5 -0.15747837979706958 1106 6 -0.3451938337878957 1106 7 -0.622627358451993 1106 8 -0.5160890624701153 1106 9 -0.7080931507590975 1106 10-11 -0.9654945789304961 1106 12-13 -0.6268013390546017 1106 14-15 -0.7124481342556876 1106 16-17 -0.5329973446099103 1106 18-19 -0.7001238502978495 1106 20-21 -0.6797120931511813 1106 22-23 -0.7456494079161118 1106 24-25 -0.6611701964811942 1106 26-27 -0.805926554990485 1106 28-29 -0.5466638060501054 1106 30-31 -0.7515267031144504 1106 32-33 -0.5087567781516498 1106 34-35 -0.339644289182921 1106 36-37 -0.7832243552001117 1106 38-39 -0.6155169952178667 1106 40-41 -0.6310217073915325 1106 42-43 -0.5371556105367432 1106 44-45 -0.37603356035615576 1106 46-47 -0.48052944223131533 1106 48-49 -0.11078327032407742 1106 50-51 -0.23966503672779282 1106 52-53 -0.21029210917729557 1106 54-55 -0.3857871642880326 1106 56-57 -0.4362339712717045 1106 58-59 -0.3215941375924416 1106 60-61 -0.5713883721433106 1106 62-63 -0.5762433615859877 1106 64-65 -0.42691952897394003 1106 66-67 -0.36351908786145515 1106 68-69 -0.49906315638013155 1106 70-71 -0.30756136374251497 1106 72-73 -0.5375834790082692 1106 74-75 -0.1655259771964417 1106 76-77 0.07446151999110029 1106 78-79 -0.01482938653268917 1106 80-81 -0.593826996658148 1106 82-83 -0.4316454991553691 1106 84-85 -0.4907370427198856 1106 86-87 -0.24040781849578963 1106 88-89 -0.09269834813533961 1106 90-91 0.07563745656738519 1106 92-93 0.010454172278446805 1106 94-95 0.17627597480919732 1106 96-97 0.29167666948133686 1106 98-99 0.49729328096368164 1106 100-101 0.8197876844511605 1107 1 -0.024043990078546074 1107 2 0.008557762418352155 1107 3 0.07799428775654249 1107 4 0.11916565118206535 1107 5 0.10201314369491143 1107 6 0.03909659346107475 1107 7 0.1288339043841944 1107 8 0.1385948251947795 1107 9 0.05190116813505341 1107 10-11 0.0733160096688934 1107 12-13 0.07055352754022692 1107 14-15 0.16267712623791653 1107 16-17 0.12490846651260767 1107 18-19 0.05090409671006313 1107 20-21 0.12220208006819178 1107 22-23 0.2074621811014623 1107 24-25 0.22478138436541428 1107 26-27 0.16205585462251548 1107 28-29 0.19179557097455557 1107 30-31 0.126528535119391 1107 32-33 0.1371347653294137 1107 34-35 0.28457576424402475 1107 36-37 0.10049511214361218 1107 38-39 0.2139724592293888 1107 40-41 0.21821791867247242 1107 42-43 0.1650634837652305 1107 44-45 0.3557910653089422 1107 46-47 0.2793125476286207 1107 48-49 0.1102465966872046 1107 50-51 0.1681925551320944 1107 52-53 0.09129351552078191 1107 54-55 0.016017814219331683 1107 56-57 0.14365088112913327 1107 58-59 0.2526251706277165 1107 60-61 0.40971055361919184 1107 62-63 0.5104235301038997 1107 64-65 0.5297120662157013 1107 66-67 0.40084301951320356 1107 68-69 0.23182426216219199 1107 70-71 0.6102866017991246 1107 72-73 0.49704102005934914 1107 74-75 0.48158156841678945 1107 76-77 0.38741921942378 1107 78-79 0.4209399255011732 1107 80-81 0.5728043092159218 1107 82-83 0.8131430944465485 1107 84-85 0.8451877106824206 1107 86-87 0.7024028209530009 1107 88-89 0.8715089315705704 1107 90-91 1.0776034874119347 1107 92-93 1.1457955602743048 1107 94-95 1.2379709870963431 1107 96-97 1.312585856828953 1107 98-99 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0.1391258411723939 2316 2 0.20152922679153917 2316 3 0.33884854428204036 2316 4 0.05105244070398385 2316 5 0.1966115167861986 2316 6 0.29746543883225485 2316 7 0.19102096264118984 2316 8 -0.10900390517435454 2316 9 0.19405374131228825 2316 10-11 -0.2724629337206821 2316 12-13 1.2318895474550118E-4 2316 14-15 -0.12354256339636294 2316 16-17 0.14276070881948755 2316 18-19 0.2638973602102439 2316 20-21 0.048109643485005904 2316 22-23 0.09252227109406164 2316 24-25 0.32523600748905324 2316 26-27 0.3458999054284604 2316 28-29 0.3809767833374096 2316 30-31 0.1830755223350593 2316 32-33 -0.06634273911066657 2316 34-35 0.13688538433857644 2316 36-37 0.051472966094642914 2316 38-39 0.0845938637973731 2316 40-41 0.05309082613751315 2316 42-43 0.38627802134930533 2316 44-45 -0.3459078167738241 2316 46-47 -0.4577503695191609 2316 48-49 -0.20079743133581474 2316 50-51 -0.6113864265742421 2316 52-53 -1.06340520604585 2316 54-55 -1.1050967891245875 2316 56-57 -0.7688911249083361 2316 58-59 -0.7041972829908829 2316 60-61 -0.5484387471000218 2316 62-63 -0.5264278681987733 2316 64-65 -0.5619750101640832 2316 66-67 -0.6286039247255673 2316 68-69 -0.5089926614165847 2316 70-71 -0.4835946266153428 2316 72-73 -0.2721846708072846 2316 74-75 -0.05140708671688188 2316 76-77 -0.40435510524340756 2316 78-79 -0.475657909084358 2316 80-81 -0.3880375202538069 2316 82-83 -0.3081281415794912 2316 84-85 -0.21349906019891662 2316 86-87 -0.058613246194521906 2316 88-89 0.1422206911898911 2316 90-91 0.5612903877417672 2316 92-93 0.34864135604599866 2316 94-95 0.5251084665787573 2316 96-97 0.5537522913513158 2316 98-99 0.4531111827565475 2316 100-101 0.2614148570552892 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6867.0 3 1502.0 4 1002.0 5 1206.0 6 1380.0 7 1581.0 8 1950.0 9 2588.0 10 3152.0 11 4281.0 12 5522.0 13 7003.0 14 8048.0 15 9941.0 16 12689.0 17 16603.0 18 14232.0 19 10222.0 20 10410.0 21 11083.0 22 12339.0 23 13717.0 24 15295.0 25 17517.0 26 20406.0 27 23912.0 28 28554.0 29 35126.0 30 42743.0 31 53380.0 32 67966.0 33 88746.0 34 120183.0 35 166782.0 36 241485.0 37 330350.0 38 258414.0 39 29434.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.70032334320572 19.501659245642784 14.97024699073445 22.82777042041705 2 14.581740259540876 20.531151223274 40.87287551615591 24.01423300102921 3 18.918377962528126 24.81034092874936 30.27767068135564 25.99361042736688 4 11.89448512045347 16.650566839867736 38.659364666981574 32.79558337269721 5 13.002640597653695 37.942825628440985 34.820696806010545 14.233836967894772 6 30.964414835588354 36.994111764834436 17.94638864950973 14.095084750067482 7 27.809712820876303 33.05211988045032 21.179253641421838 17.95891365725154 8 25.927614556935485 34.79953532899333 20.23887916723018 19.033970946841 9 25.867288244895835 16.78816339932582 20.971414803073717 36.37313355270462 10-11 24.296314102185505 26.937004032705786 28.063096371509772 20.703585493598936 12-13 25.35616094499815 24.276471022517534 28.455164267257288 21.91220376522702 14-15 22.15550209284088 25.664110849702844 27.126678288311272 25.053708769145004 16-17 21.404014219809337 29.126334583679586 27.50091398716343 21.96873720934765 18-19 21.19961755672131 27.78707901563966 29.879503201692764 21.13380022594626 20-21 22.20882092248314 26.681994341731762 29.81618600124834 21.292998734536752 22-23 21.692590403385193 26.693910160833262 29.804109022003633 21.809390413777912 24-25 21.63839496069241 26.805332653111837 29.915220310280198 21.641052075915553 26-27 21.440111772618778 26.968775945738788 29.88996349438243 21.70114878726 28-29 21.175752836647384 27.384927675236653 29.94301900075361 21.49630048736236 30-31 21.46870732871243 26.84753386535127 29.87010801003558 21.81365079590072 32-33 21.215076911264866 26.99820161411777 29.915041770391305 21.87167970422606 34-35 21.72911879883995 26.975954354027987 29.701895418273743 21.59303142885832 36-37 21.64844813801362 26.726616963308246 29.776281743168347 21.848653155509787 38-39 21.413682620551683 27.134742953376012 29.782740657547123 21.66883376852518 40-41 21.639512013372926 27.106156163514843 29.640357563987575 21.613974259124653 42-43 21.377165790700538 27.105839600322064 29.348787263498743 22.16820734547866 44-45 21.55905081174047 27.06130341516887 29.29480046682165 22.084845306269017 46-47 21.711467845745577 27.388689087830237 28.948574574161896 21.951268492262294 48-49 21.763348029278482 27.412308681003484 28.800094980224266 22.024248309493768 50-51 21.564695948490264 27.563237557380955 28.889138943797597 21.98292755033118 52-53 21.8632512515505 27.82930762456941 28.382066530170587 21.925374593709503 54-55 21.623878160259615 27.420468547140008 28.626184155966587 22.32946913663379 56-57 21.7726868511273 27.686957415173417 28.4657818004457 22.07457393325358 58-59 22.111322631122647 27.39728938859959 28.468090997621065 22.023296982656703 60-61 21.831671478077087 27.39687937233232 28.992083311194943 21.779365838395645 62-63 21.57829279143246 27.53489833011708 28.985621477657602 21.90118740079286 64-65 21.522155846287266 27.622710369699988 28.79011409852015 22.065019685492597 66-67 21.51523962316783 27.587031376606358 28.466267085631465 22.43146191459435 68-69 21.463085746605064 27.5080653945645 28.712952245838597 22.315896612991832 70-71 21.666988919900636 27.43655178215878 28.61933430845686 22.27712498948372 72-73 21.658884146625866 27.217988691385344 28.805410233959734 22.317716928029057 74-75 21.418934916133665 27.86172578846282 28.407776551670388 22.311562743733123 76-77 21.513271723404884 27.453128229354277 28.450146151347838 22.583453895892998 78-79 21.633965721209446 27.42515815109582 28.454590625810976 22.48628550188376 80-81 21.70540713285124 27.56948411485895 28.318018708198455 22.407090044091362 82-83 21.620997705088186 27.408845595003267 28.616729653248097 22.35342704666045 84-85 21.60154522584138 27.454710984553053 28.448049367875573 22.49569442172999 86-87 21.52540466397988 27.446885961325503 28.521876373740735 22.50583300095389 88-89 21.539842263517052 27.367028853406627 28.27536426558655 22.817764617489765 90-91 21.513781187480365 27.272703115736462 28.460945231914625 22.75257046486854 92-93 21.64854045628454 27.228283973359783 28.543910301827975 22.579265268527703 94-95 21.46173166348236 27.131929405263193 28.473204607126075 22.93313432412837 96-97 21.291756690606785 27.128201373343625 28.621619562913224 22.958422373136365 98-99 21.422088478392965 27.10828112737702 28.274182985887414 23.1954474083426 100-101 21.4457004104799 26.608873993356045 28.8661001929899 23.07932540317415 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2591.0 1 1520.0 2 420.5 3 370.5 4 302.0 5 283.0 6 438.0 7 895.0 8 1496.0 9 1760.5 10 1662.0 11 1628.0 12 1748.0 13 1856.5 14 1993.5 15 2139.5 16 2228.5 17 2356.0 18 2496.5 19 2728.5 20 3030.0 21 3499.0 22 4172.0 23 5048.0 24 6227.5 25 7843.5 26 10054.0 27 12639.0 28 15040.5 29 17886.5 30 21507.5 31 25058.5 32 28801.5 33 32525.5 34 36913.5 35 41006.5 36 45056.0 37 48945.5 38 51761.5 39 53586.0 40 54586.5 41 56915.5 42 59227.0 43 61384.0 44 63596.5 45 64952.0 46 66115.0 47 69507.5 48 75206.0 49 74087.5 50 66226.0 51 59869.5 52 55360.0 53 52182.0 54 49319.5 55 44830.5 56 39073.0 57 33784.0 58 29921.5 59 26715.0 60 22839.5 61 18188.5 62 13867.0 63 10373.0 64 7865.0 65 6047.0 66 4322.5 67 3147.0 68 2285.0 69 1528.0 70 1120.5 71 806.0 72 542.0 73 429.5 74 345.0 75 255.0 76 187.0 77 113.5 78 73.5 79 53.0 80 40.5 81 30.5 82 20.0 83 15.5 84 9.5 85 8.0 86 6.5 87 3.5 88 3.0 89 2.0 90 3.0 91 2.5 92 1.5 93 3.0 94 2.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27568039452927573 2 0.24086690880987363 3 0.23097068951907906 4 0.23668457803816878 5 0.2392764449952816 6 0.2698486938757718 7 0.2708500970182927 8 0.25783185616552134 9 0.2529426525873312 10-11 0.2628388718781257 12-13 0.2646649599615461 14-15 0.2489370400172477 16-17 0.26431152355830345 18-19 0.2530015586545383 20-21 0.24546158205202817 22-23 0.24298752722932956 24-25 0.23868738432321052 26-27 0.24622736092572065 28-29 0.2604826291898413 30-31 0.2616607505339835 32-33 0.2611305959291195 34-35 0.27026103634622156 36-37 0.24175049981798027 38-39 0.24222174835563715 40-41 0.23839285398717497 42-43 0.2447252562119393 44-45 0.26307449614695416 46-47 0.2677280754563159 48-49 0.27297071543774865 50-51 0.2742961019499086 52-53 0.2955611922116754 54-55 0.2733241518409913 56-57 0.24175049981798027 58-59 0.22346016595017254 60-61 0.21960181854810681 62-63 0.22834936952836268 64-65 0.21977853674972817 66-67 0.2215162657323379 68-69 0.22428485089107209 70-71 0.22475609942872896 72-73 0.22767194975548088 74-75 0.230381628847008 76-77 0.2455204881192353 78-79 0.2393648040960923 80-81 0.2341810701818666 82-83 0.2280253861587236 84-85 0.22885007109962313 86-87 0.23689074927339365 88-89 0.23388653984583105 90-91 0.24446017890950733 92-93 0.23197209266159996 94-95 0.22938022570448713 96-97 0.24074909667545938 98-99 0.24534376991761397 100-101 0.22072103382504193 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1697618.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.621443270925724 #Duplication Level Percentage of deduplicated Percentage of total 1 79.36808809503196 47.320399618797836 2 11.91226899437252 14.204533401519774 3 3.8158245961865687 6.825149090800216 4 1.6402533040981204 3.911770772809383 5 0.8624130260154539 2.570915465334389 6 0.5180610693896587 1.8532529195694394 7 0.3350399692313723 1.3982896563314675 8 0.23327370915136636 1.1126492173413292 9 0.18488109756425583 0.9920590083264387 >10 1.0200662763722004 11.162763725825561 >50 0.07642997717517601 3.1467187180331093 >100 0.031134119571565824 3.1790380704806624 >500 9.851155825032142E-4 0.38135994522415956 >1k 0.001182138699003857 1.5511065444978946 >5k 9.851155825032144E-5 0.38999384510833507 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6640 0.39113628625521174 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4716 0.27780101294873166 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3578 0.2107659084670403 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3397 0.20010391030255334 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2707 0.1594587239296473 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2650 0.15610107809884202 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2039 0.12010947103529769 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 5.89060672071102E-5 0.0 0.0 0.0 0.0 3 5.89060672071102E-5 0.0 0.0 5.89060672071102E-5 0.0 4 5.89060672071102E-5 0.0 0.0 5.89060672071102E-5 0.0 5 1.767182016213306E-4 0.0 0.0 5.89060672071102E-5 0.0 6 2.356242688284408E-4 0.0 0.0 5.89060672071102E-5 0.0 7 3.534364032426612E-4 0.0 0.0 5.89060672071102E-5 0.0 8 3.534364032426612E-4 0.0 0.0 5.89060672071102E-5 0.0 9 3.534364032426612E-4 0.0 0.0 5.89060672071102E-5 0.0 10-11 4.123424704497714E-4 0.0 0.0 5.89060672071102E-5 0.0 12-13 5.301546048639918E-4 0.0 0.0 5.89060672071102E-5 0.0 14-15 6.479667392782121E-4 0.0 0.0 5.89060672071102E-5 0.0 16-17 6.774197728817673E-4 0.0 0.0 5.89060672071102E-5 0.0 18-19 7.952319072959877E-4 0.0 0.0 5.89060672071102E-5 0.0 20-21 8.83591008106653E-4 2.94530336035551E-5 0.0 5.89060672071102E-5 0.0 22-23 9.424970753137632E-4 5.89060672071102E-5 0.0 5.89060672071102E-5 0.0 24-25 0.0015315577473848652 5.89060672071102E-5 0.0 1.767182016213306E-4 0.0 26-27 0.0015315577473848652 5.89060672071102E-5 0.0 3.2398336963910607E-4 0.0 28-29 0.0016788229154026408 5.89060672071102E-5 0.0 0.0012370274113493141 0.0 30-31 0.001767182016213306 5.89060672071102E-5 0.0 0.0033281927972017263 0.0 32-33 0.001767182016213306 5.89060672071102E-5 0.0 0.005978965821521685 0.0 34-35 0.002032259318645302 5.89060672071102E-5 0.0 0.009395517719534077 0.0 36-37 0.0024740548226986282 8.83591008106653E-5 0.0 0.015227218373037987 0.0 38-39 0.002739132125130624 1.178121344142204E-4 0.0 0.025035078563021833 0.0 40-41 0.0027685851587341795 1.178121344142204E-4 0.0 0.04170549558263402 0.0 42-43 0.0028863972931484 1.178121344142204E-4 0.0 0.05796357013179644 0.0 44-45 0.003239833696391061 1.178121344142204E-4 0.0 0.07831561635185301 0.0 46-47 0.0037110822340479426 1.178121344142204E-4 0.0 0.11218660499594138 0.0 48-49 0.003828894368462163 1.178121344142204E-4 0.0 0.14134510826346092 0.0 50-51 0.003828894368462163 1.178121344142204E-4 0.0 0.18084162632582831 0.0 52-53 0.003828894368462163 1.178121344142204E-4 0.0 0.22513898886557518 0.0 54-55 0.003828894368462163 1.178121344142204E-4 0.0 0.279038040360081 0.0 56-57 0.003887800435669273 1.178121344142204E-4 0.0 0.3721685326145222 0.0 58-59 0.003887800435669273 1.178121344142204E-4 0.0 0.4542835903012339 0.0 60-61 0.003917253469272828 1.178121344142204E-4 0.0 0.5360746646183063 0.0 62-63 0.0039761595364799385 1.178121344142204E-4 0.0 0.627261256654913 0.0 64-65 0.004005612570083494 1.178121344142204E-4 0.0 0.7256638419243906 0.0 66-67 0.004064518637290604 1.178121344142204E-4 0.0 0.8589682720140809 0.0 68-69 0.004064518637290604 1.178121344142204E-4 0.0 1.0289417289401974 0.0 70-71 0.004182330771704824 1.178121344142204E-4 0.0 1.179181653351932 0.0 72-73 0.0045063141413439305 1.178121344142204E-4 0.0 1.3483598783707524 0.0 74-75 0.004800844477379482 1.178121344142204E-4 0.0 1.5546194726964488 0.0 76-77 0.004830297510983036 1.178121344142204E-4 0.0 1.7882114822062443 0.0 78-79 0.0048597505445865915 1.178121344142204E-4 0.0 2.0571765850739094 0.0 80-81 0.005360452115847028 1.178121344142204E-4 0.0 2.351147313470993 0.0 82-83 0.005537170317468359 1.178121344142204E-4 0.0 2.6697996840278555 0.0 84-85 0.005566623351071914 1.178121344142204E-4 0.0 3.007832150695857 0.0 86-87 0.005625529418279024 1.178121344142204E-4 0.0 3.3326991113430697 0.0 88-89 0.005654982451882579 1.767182016213306E-4 0.0 3.7443641620199597 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10380 0.0 29.025267 1 GTATAGG 435 0.0 27.31084 1 GTACATG 7110 0.0 26.935171 1 TACATGG 7115 0.0 26.176142 2 ATCAACG 11915 0.0 24.881985 3 TCAACGC 11980 0.0 24.78884 4 CAACGCA 12065 0.0 24.613468 5 TATCAAC 12155 0.0 24.468866 2 AACGCAG 12145 0.0 24.332535 6 ACATGGG 7305 0.0 24.324608 3 GGTATCA 7330 0.0 23.339031 1 GAGTACT 5945 0.0 22.33401 12-13 CATGGGG 3460 0.0 21.972368 4 CGCAGAG 13590 0.0 21.463717 8 ACGCAGA 13690 0.0 21.411669 7 AGTACTT 6275 0.0 21.045916 12-13 GTACTTT 6390 0.0 20.925808 14-15 AGAGTAC 9670 0.0 20.608608 10-11 GCAGAGT 14235 0.0 19.92383 9 GCACCGT 315 3.2014214E-10 19.607729 6 >>END_MODULE