FastQCFastQC Report
Thu 26 May 2016
SRR937344_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937344_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1697618
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74200.43708301867675764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56220.3311699098383735No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49640.292409717616095No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40920.24104362701149493No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT17320.10202530840271486No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT16980.10002250211767312No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG601.7121753E-423.74918280-81
GTACATG72150.023.719371
TACATGG71700.022.9211082
GAGTACT64950.022.48966612-13
TCGCGGA1507.2759576E-1222.16590382-83
GTACTTT67500.021.60232414-15
ACATGGG72450.021.5037463
ATTCGCG1451.05501385E-1021.29237280-81
GTATCAA129800.021.131881
GGTATCA101250.020.6583021
CGCGGAA1501.6734703E-1020.58262482-83
AGTACTT67350.020.20710412-13
TATCGTT1200.0047621219.7933187
AATTCGC1803.6379788E-1219.79098578-79
AGAGTAC105100.019.39029510-11
AGGATCG2051.5901898E-518.5364935
CATGGGG34150.018.498634
TCAACGC147800.018.3823184
ATCAACG147850.018.37613
CAACGCA148050.018.3518165
Produced by FastQC (version 0.11.5)